RE: [Histonet] type of paraffin and polymer
Ester Wax Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: 11 September 2009 17:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] type of paraffin and polymer Dear Histonetters, I quite need answer as soon as it possible, PLEASE! I am working with mouse tissue and tumors. What type of paraffin is the best for processing and embedding to cut 4µ nice sections? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re:[Histonet] formalin storage
I think HCl and formalin when combined form a carcinogen, at least that is what I was taught many years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ti fei Sent: Friday, September 11, 2009 10:23 AM To: Riesen, Rebecca; histonet Subject: Re:[Histonet] formalin storage never store everything in one cabinet. As far as i know, HCl and formalin should not be stored together. If any of you know why... -- Original -- From: Riesen, Rebeccarebecca.rie...@nchmd.org; Date: Fri, Sep 11, 2009 10:09 PM To: histonethistonet@lists.utsouthwestern.edu; Subject: [Histonet] formalin storage Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AAVLD Histo sample block slide retention policy
I disagree, the slide is what was used to make the diagnosis. The block may not be a good representation of the actual slide, it may could have been cut so that what is on the original slide is no longer in the block. In Surgical path we keep our slides indefinitely and our blocks for 10 years. Cheryl A. Miller HT ASCP Histology Supervisor Physcians Laboratory, P.C. 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn [lynn.bur...@illinois.gov] Sent: Friday, September 11, 2009 10:35 AM To: Jan Shivers; histonet Subject: RE: [Histonet] AAVLD Histo sample block slide retention policy I read our SOP's on storage and referred to Dr. webb, our pathologist. His feeling was that they, AAVLD, leave that up to each lab depending on the state statutes regarding law suits. Our SOP says seven years on blocks and nine years on slides. I would agree with you that as long as you have blocks, the slides would be less important. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/11/2009 9:21 AM To: histonet Subject: [Histonet] AAVLD Histo sample block slide retention policy For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Permount question
I do recall having to do that in the past; if the Permount bottle had been left open in the hood and became too viscous we would add just enough xylene to get it flowing again. Didn't happen often, if you're careful to keep the bottle closed when not in use. Regards, Merced --On Monday, September 14, 2009 9:15 AM +1000 Tony Henwood antho...@chw.edu.au wrote: It is possible that the mountant was too thick and so xylene was added to thin it? It would save the lab money in mountant costs but there is a possibility that with less mountant on the slide you may have shrinkage of the mountant causing eventual cracking of the mountant and possible detachment of the coverslip. I would review a sample of slides one and two years old and record the quality (another audit!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Saturday, 12 September 2009 12:30 AM To: Leslie Chen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Permount question Just so a stereotype doesn't get started here...I was 1st taught in the (straight) use of Permount by such an Asian technician (who couldn't speak English, but was such a wonderful teacher I learned more from watching her than anything!) :-) --On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen lc...@mednet.ucla.edu wrote: Thanks for all the replies, I guess these weird Asian technicians who are highly looked up to taught me something wrong and it's been propagated in the department. Thanks again! On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen lc...@mednet.ucla.edu wrote: Hello, Did anyone ever use Permount straight without cutting it with some solvent? Journal articles mention using Permount to coverslip, and the post-doc I work with thinks that it isn't diluted. I think maybe people just universally know that it should be diluted so it isn't mentioned in the Materials and Methods. Any thoughts? Leslie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DNA Extraction
Hello Histo land, Does anyone know how to extract DNA from formalin fixed paraffin embedded tissue? Thanks, Sharon WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Company email
I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Where were you going to have the product delivered?.. Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Mike Pence Sent: Mon 9/14/2009 12:38 PM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Company email Why would you think your email address is your business address? Just have to ask? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AAVLD Histo sample block slide retention policy
CLIA (feds) require blocks to be retained 2 years and slides to be retained 10 years. Space usually determines how long beyond that they are kept. We keep a Cancer file of slides and blocks that are retained indefinitely. K Black Digestive Health Specialists Tacoma, WA - Original Message - From: Cheri Miller cmil...@physlab.com To: Burton, Lynn lynn.bur...@illinois.gov; Jan Shivers shive...@umn.edu; histonet histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 6:19 AM Subject: RE: [Histonet] AAVLD Histo sample block slide retention policy I disagree, the slide is what was used to make the diagnosis. The block may not be a good representation of the actual slide, it may could have been cut so that what is on the original slide is no longer in the block. In Surgical path we keep our slides indefinitely and our blocks for 10 years. Cheryl A. Miller HT ASCP Histology Supervisor Physcians Laboratory, P.C. 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn [lynn.bur...@illinois.gov] Sent: Friday, September 11, 2009 10:35 AM To: Jan Shivers; histonet Subject: RE: [Histonet] AAVLD Histo sample block slide retention policy I read our SOP's on storage and referred to Dr. webb, our pathologist. His feeling was that they, AAVLD, leave that up to each lab depending on the state statutes regarding law suits. Our SOP says seven years on blocks and nine years on slides. I would agree with you that as long as you have blocks, the slides would be less important. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/11/2009 9:21 AM To: histonet Subject: [Histonet] AAVLD Histo sample block slide retention policy For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Why would you think your email address is your business address? Just have to ask? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AAVLD Histo sample block slide retention policy
CAP requires 10 years for each. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Konni Black Sent: Monday, September 14, 2009 10:41 AM To: Cheri Miller; Burton, Lynn; Jan Shivers; histonet Subject: Re: [Histonet] AAVLD Histo sample block slide retention policy CLIA (feds) require blocks to be retained 2 years and slides to be retained 10 years. Space usually determines how long beyond that they are kept. We keep a Cancer file of slides and blocks that are retained indefinitely. K Black Digestive Health Specialists Tacoma, WA - Original Message - From: Cheri Miller cmil...@physlab.com To: Burton, Lynn lynn.bur...@illinois.gov; Jan Shivers shive...@umn.edu; histonet histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 6:19 AM Subject: RE: [Histonet] AAVLD Histo sample block slide retention policy I disagree, the slide is what was used to make the diagnosis. The block may not be a good representation of the actual slide, it may could have been cut so that what is on the original slide is no longer in the block. In Surgical path we keep our slides indefinitely and our blocks for 10 years. Cheryl A. Miller HT ASCP Histology Supervisor Physcians Laboratory, P.C. 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn [lynn.bur...@illinois.gov] Sent: Friday, September 11, 2009 10:35 AM To: Jan Shivers; histonet Subject: RE: [Histonet] AAVLD Histo sample block slide retention policy I read our SOP's on storage and referred to Dr. webb, our pathologist. His feeling was that they, AAVLD, leave that up to each lab depending on the state statutes regarding law suits. Our SOP says seven years on blocks and nine years on slides. I would agree with you that as long as you have blocks, the slides would be less important. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/11/2009 9:21 AM To: histonet Subject: [Histonet] AAVLD Histo sample block slide retention policy For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. --
RE: [Histonet] DNA Extraction
Sharon: Here is just one of many such protocols published recently. Commercial kits are available from molecular diagnostic suppliers (we use a Qiagen kit for example - there are certainly numerous others). http://www.protocol-online.org/prot/Protocols/DNA-Extraction-from-Archiv al-Formalin-fixed--Paraffin-embedded-Tissue-Sections-3159.html Joe 380 MRC 4-4737 (voice) 3-3482 (fax) pager 131-1170 joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Monday, September 14, 2009 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DNA Extraction Hello Histo land, Does anyone know how to extract DNA from formalin fixed paraffin embedded tissue? Thanks, Sharon WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Company email
I've jumped through hoops already and made a dozen calls to the company (they are refusing) to no evail so I suppose hurt is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Galbraith, Joe joseph-galbra...@uiowa.edu To: Sheila Haas micropathl...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Company email
Sheila, Joe is correct about the generic emails such as yahoo, gmail, etc. Look into creating your own website which will then come with email in the y...@yourcompany.com format. Websites can be hosted for as little as $10-12/month and the name searched for conflicts. Some even has easy, free software to design the website yourself. Paula From: Galbraith, Joe joseph-galbra...@uiowa.edu To: Sheila Haas micropathl...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 12:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Sometimes the best way to complain is with the feet. Joe joseph-galbra...@uiowa.edu From: Sheila Haas [mailto:micropathl...@yahoo.com] Sent: Monday, September 14, 2009 12:33 PM To: Galbraith, Joe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email I've jumped through hoops already and made a dozen calls to the company (they are refusing) to no evail so I suppose hurt is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Galbraith, Joe joseph-galbra...@uiowa.edu To: Sheila Haas micropathl...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathl...@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Talk about being ridiculous. Almost sounds like Navy logic Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: Sheila Haas [micropathl...@yahoo.com] Sent: Monday, September 14, 2009 1:49 PM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email Yes, it was a company card. They indicated payment wasn't the issue they needed the email address for their records. Go figure! Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Podawiltz, Thomas tpodawi...@lrgh.org To: Sheila Haas micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:41:06 PM Subject: RE: [Histonet] Company email Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathl...@yahoo.commailto:micropathl...@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Company email
Thanks to all for listening and the input. I knew it wasn't just me! Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Podawiltz, Thomas tpodawi...@lrgh.org To: Sheila Haas micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 2:04:57 PM Subject: RE: [Histonet] Company email Talk about being ridiculous. Almost sounds like Navy logic Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: Sheila Haas [micropathl...@yahoo.com] Sent: Monday, September 14, 2009 1:49 PM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email Yes, it was a company card. They indicated payment wasn't the issue they needed the email address for their records. Go figure! Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Podawiltz, Thomas tpodawi...@lrgh.org To: Sheila Haas micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:41:06 PM Subject: RE: [Histonet] Company email Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathl...@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
I imagine there are some regulations pertaining to lab supplies, chemicals etc post-9/11 also. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Galbraith, Joe Sent: Mon 9/14/2009 1:36 PM To: Sheila Haas Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Company email Sometimes the best way to complain is with the feet. Joe joseph-galbra...@uiowa.edu From: Sheila Haas [mailto:micropathl...@yahoo.com] Sent: Monday, September 14, 2009 12:33 PM To: Galbraith, Joe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email I've jumped through hoops already and made a dozen calls to the company (they are refusing) to no evail so I suppose hurt is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Galbraith, Joe joseph-galbra...@uiowa.edu To: Sheila Haas micropathl...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet :: ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
