[Histonet] BLTS - OT
Hey, I thought you meant that famous sandwich ...Bacon Lettuce and Tomato - guess it must be lunchtime On Tue, Sep 29, 2009 at 3:20 PM, Kathleen Jones kjo...@upei.ca wrote: Hello Histonet I am wondering if anyone out there is familiar with the Barbeito-Lopez Trichrome stain. I am using it to identify early myocardial infarction. My damaged tissue is showing up, but it is very subtle. The papers I've read show the necrotic myofibers 'popping' out as yellow, whereas mine are more pale, almost pink. I'm not sure if I am under decolorizing or if I should leave it in the molybdic acid aniline blue methyl orange longer. Any suggestions would be greatly appreciated! Kathy Jones Research Technician AVC-UPEI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] digest
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Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staining of plastic sections
Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried HE and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staining of plastic sections
Hi Lesley: There is a fairly large literature on this subject. I my opinion, most of the fancier stains are not worth the effort and the results are disappointing. In a separate mailing, I will send you some references. Geoff Lesley Bechtold wrote: Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried HE and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pasturella
Hello all, We're looking for a place that will do IHC for Pasturella multocida or where we can purchase some antibody to do the test ourselves. Anyone have any ideas? Thanks Denise Long Woodward, MS, HTL (ASCP) QIHC University of Connecticut ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staining of plastic sections
I use McNeal as a substitute for HE. I use it on MMA embedded bone. It is to intense (for us) to be used on JB-4. I think you might need to try a few different stains to select the best. */ /* */-- You are what you do - not what you say --/* */Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 3525 John Hopkins Ct. 0863 San Diego, CA 92121-08630 858-822-1251 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* Walters, Katherine S wrote: Perhaps you could try the Paragon stain. It looks more like an HE. Kathy Walters -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Wednesday, September 30, 2009 10:12 AM To: histonet@lists.utsouthwestern.edu Cc: Pete Finger Subject: [Histonet] staining of plastic sections Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried HE and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] discarding old dry chemicals with no expiration date
Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
Our dilemma has been that we aren't sure how they should be disposed of. We have chemicals that are probably more than 30 years old. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kelly Boyd Sent: Wed 9/30/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
My laboratory had many chemicals that were over 10 years old when the facility started to prepare for ISO 17025 accreditation status four years ago. The facility's current policy for stock chemicals without expiration dates is to set a 5-year expiration period from the date the substance is received by the laboratory. Prepared reagents and working solutions have one-year expiration periods from the dates they are made. Histochemical dyes have been shown worldwide to last for decades in their dried forms. In an emergency, dyes older than five years would probably be acceptable. If a dye can no longer be obtained, the main reason an expired dye is being kept, the method using the dye needs to be updated to use dyes that are currently being manufactured. A good method is one that can reproduced reliably based on readily-available reagents. :o) Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.o...@fda.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
Hi Kelly and Lynn, Can you not ask the surveyor for their reference research literature to indicate that 10 years and older, the chemicals in your list would not be good at all? And if you need to dispose of them, could the surveyor please let you know how to dispose of it? If they want it disposed, they should have a knowledge of how to do it. Just thinking ... Agripina C. Suarez University of British Columbia Shaw Laboratory VGH Research Pavilion, Room 386 828 W. 10th Ave, Vancouver B.C., Canada V5Z 1L8 Phone: 604-875-4111 x68375 Fax: 604-875-4376 email: sua...@interchange.ubc.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Wednesday, September 30, 2009 10:32 AM To: Kelly Boyd; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date Our dilemma has been that we aren't sure how they should be disposed of. We have chemicals that are probably more than 30 years old. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kelly Boyd Sent: Wed 9/30/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.409 / Virus Database: 270.13.115/2405 - Release Date: 09/30/09 10:35:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
When we renovated our lab, we also had many chemicals to discard. We used the same company that hauls our waste xylene away. The company we used was Safety-Kleen, based in Saginaw, Michigan. They came in, made a list of all the chemicals and then gave us a lovely price for disposal/hauling away. Wasn't cheap, but, most importantly, it was disposed of properly, safely, and not down our drains for our fish to grow extra fins! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Burton, Lynn lynn.bur...@illinois.gov 9/30/2009 1:31 PM Our dilemma has been that we aren't sure how they should be disposed of. We have chemicals that are probably more than 30 years old. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kelly Boyd Sent: Wed 9/30/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] staining of plastic sections
Under separate cover I sent an article for Stains for Plastic Embedded Tissue Sections which originally appeared in Stain Technology 42:9-14 (1974) by Humphrey and Pittman. I received it as an LKB applicaton note 303. The article describes an HE stain for plastic sections, using methylene blue-azure II and basic fuchsin. I used it a lot, with great success, when I worked at the Mass. Eye and Ear Infirmary. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Wednesday, September 30, 2009 11:12 AM To: histonet@lists.utsouthwestern.edu Cc: Pete Finger Subject: [Histonet] staining of plastic sections Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried HE and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part Time/Per Diem histotech needed Napa CA
HISTOTECH Part Time Per Diem Position Available.. Mon-Fri 20-40 hours a week depending on amount of work and needs of the lab ( 330am- 730am 30-80 blocks... Embed and cut Path Asst. will stain, coverslip, QC and hand out the slides. Call me if you would like more info. Please, serious applicants only! Requirements: * work the shift as stated above..YES 330am * have 5+ years experience * HT or HTL license (or can sit for the exam) Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center Napa CA 94558 707-294-9229 cell 707-252-4411 x3689 Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN-4
In response to the use of Pin 4 staining on various platforms, I have used the PIN 4 from Diagnostic Biosystems on the Ventana Benchmark XT and the results were fantastic! Martha Brereton 860-442-0711 This message (and any included attachments) is from Lawrence Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor Position In TX
Allied Search Partners, is now accepting resumes for a qualified Histology Supervisor in Corpus Christi, TX area. To apply for this position please send resume to aly...@alliedsearchpartners.com for intial prescreening of resume. Upon receipt of resume one of our recruiters will hold a confidential phone interview with you. *All inquiries and resume submissions are confidential* POSITION: Histology Supervisor REQUIREMENTS: ASCP Certification At least 3 years experience as a supervisor SHIFT: Monday-Friday TIME: Split Shift, 1-2 days per week histology supervisor will come in from 3am-11am, the remaining days histology supervisor will come in 5am-1pm. OFFER PACKAGE: Benefits package includes medical/dental insurance, PTO, 401K. Relocation expenses ARE offered To apply for this position please send resume to aly...@alliedsearchpartners.com for intial prescreening of resume. Upon receipt of resume one of our recruiters will hold a confidential phone interview with you. *All inquiries and resume submissions are confidential* *If you know someone interested send a referral and receive a cash bonus* -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
What CLIA regulation did they use for reference for that statement? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhi...@yahoo.com] Sent: Wednesday, September 30, 2009 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] discarding old dry chemicals with no expiration date
I was going to comment about how important some people seem to feel to find something to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may hurt some feelings! René J. --- On Wed, 9/30/09, Kelly Boyd kdboydhi...@yahoo.com wrote: From: Kelly Boyd kdboydhi...@yahoo.com Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
I always have a problem determining the expiration date of sodium chloride! Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about how important some people seem to feel to find something to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may hurt some feelings! René J. --- On Wed, 9/30/09, Kelly Boyd kdboydhi...@yahoo.com wrote: From: Kelly Boyd kdboydhi...@yahoo.com Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
The problem is letting people with little or no knowledge of chemistry make chemical decisions. Some chemicals are unstable on the shelf, some are not. Methyl green decomposes in a year or two, tetracycline decomposes in 5 years, indigo lasts for millennia. Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, September 30, 2009 4:40 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date I always have a problem determining the expiration date of sodium chloride! Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about how important some people seem to feel to find something to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may hurt some feelings! René J. --- On Wed, 9/30/09, Kelly Boyd kdboydhi...@yahoo.com wrote: From: Kelly Boyd kdboydhi...@yahoo.com Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Non specific Esterase protocol
Can somebody share a Non specific esterase Enzyme hsitochemistry protocol that works well with frozen muscle tissue. I have a protocol that I have used for ages but it does not give me a very strong reddish brown staining on my angular fibers. I will be happy to have it and compare. Thank you. Reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare
We have some Biocare Intellipath reagents that we are looking to get rid of/donate. No charge but I can not pay to ship them. We are located in metro Detroit. If anyone is interested please send me an email or call. Thanks Tony bor...@trinity-health.org 248-858-6231 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
