[Histonet] Sticky paraffin
We have been having some issues with sticky paraffin. We have tried all different kinds or paraffin and have come to the conclusion that it isn't the paraffin, but i'm thinking it might be another factor causing this. This does not happen everyday, but sporadic. Does anyone have any suggestions on something we can do to reduce the stickiness? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open. -John Moe Maturity is accepting imperfections. CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Diff-Quik
Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of HE and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 25, 2010 7:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik Thanks, John Kiernan, for your explanation of Romanovsky stains. Diff-Quik (please note the spelling) is the trademarked name of a staining sequence consisting of a fixative, eosin (Diff-Quik I), and an azure (Diff-Quik II), done in that order in three separate containers. I'm not sure who the trademark presently belongs to - it seems to change with the phases of the Moon. There are a number of generic equivalents, which in my personal experience all work as well as trademark Diff-Quik. For most ordinary pathology services, it isn't worthwhile to try to brew your own. I don't think I've seen bone marrow stained with such a sequence. Proper staining of bone marrows requires that the histotechnologist examine the slides under a microscope, a practice too many find abhorrent. Bob Richmond Samurai Pathoogist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
We have the Leica ST5020 and CV5030. Size was also a key reason for our decision. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Linke, Noelle Sent: Monday, January 25, 2010 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainer/coverslippers Hi all, Is anyone using a slide stainer and coverslipper combo which is NOT Sakura that they are pleased with? The new Sakura coverslipper is enormous and too big for the needs of the smaller lab we are setting up. Thank you! Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu Pager: 97471 IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Simple dye for cornea and sclera.
Hello everyone, I am trying to reconstruct the 3D geometry of the cornea and sclera of a chicken eye. I embedded and froze an eye using OCT and CO2 liquid withdrawal and sliced it using a microtome. I took pictures from the top of the eye (not the sections but what is left after slicing) using a digital camera. The problem is that the sclera and cornea are difficult to see. Is there a dye I could use before taking a picture that can be applied in one step and do not require a lengthy protocol? I cannot afford to wash the eye with tap water because it is still frozen on the microtome. Also, is anyone aware of a dye that would permeate through the sclera and cornea so that I could just dip the eye in before slicing it? Thank you. Reno Genest MSc. candidate Mechanical engineering University of Waterloo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone marrow processing on Peloris
Good morning, I am currently looking at the possibility of running bone marrow cores and clots on our Peloris. Has anyone had success with this, and if so would you be willing to share your protocol? We use xylene on our runs. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] removing trigeminal nerve and ganglion
Does anyone have experience/a good method for removing trigeminal nerve and ganglion from rat intact? Any advice would be helpful. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sticky paraffin
If you carry-on an excess of xylene (or whatever clearing agent you are using) into the paraffin this problem may occur. René J. --- On Tue, 1/26/10, Evans, Andria B aev...@wellspan.org wrote: From: Evans, Andria B aev...@wellspan.org Subject: [Histonet] Sticky paraffin To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 7:48 AM We have been having some issues with sticky paraffin. We have tried all different kinds or paraffin and have come to the conclusion that it isn't the paraffin, but i'm thinking it might be another factor causing this. This does not happen everyday, but sporadic. Does anyone have any suggestions on something we can do to reduce the stickiness? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open. -John Moe Maturity is accepting imperfections. CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Simple dye for cornea and sclera.
IF you can get hold of it, a phase microscope will be the best solution. You could also try to use oblique illumination by off-centering the condenser diaphragm. René J. --- On Tue, 1/26/10, Reno Genest rgen...@uwaterloo.ca wrote: From: Reno Genest rgen...@uwaterloo.ca Subject: [Histonet] Simple dye for cornea and sclera. To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 10:36 AM Hello everyone, I am trying to reconstruct the 3D geometry of the cornea and sclera of a chicken eye. I embedded and froze an eye using OCT and CO2 liquid withdrawal and sliced it using a microtome. I took pictures from the top of the eye (not the sections but what is left after slicing) using a digital camera. The problem is that the sclera and cornea are difficult to see. Is there a dye I could use before taking a picture that can be applied in one step and do not require a lengthy protocol? I cannot afford to wash the eye with tap water because it is still frozen on the microtome. Also, is anyone aware of a dye that would permeate through the sclera and cornea so that I could just dip the eye in before slicing it? Thank you. Reno Genest MSc. candidate Mechanical engineering University of Waterloo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
As I mentioned in a previous email, we purchased refurbished Leica 5030 and Leica XL stainer, not only for price, but size as well. Happy with both. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 26, 2010 10:01 AM To: Linke, Noelle; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE stainer/coverslippers We have the Leica ST5020 and CV5030. Size was also a key reason for our decision. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Linke, Noelle Sent: Monday, January 25, 2010 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainer/coverslippers Hi all, Is anyone using a slide stainer and coverslipper combo which is NOT Sakura that they are pleased with? The new Sakura coverslipper is enormous and too big for the needs of the smaller lab we are setting up. Thank you! Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu Pager: 97471 IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bone marrow processing on Peloris
A good tissue processor can handle those specimens without the need of any protocol modification. René J. --- On Tue, 1/26/10, debra.or...@uchospitals.edu debra.or...@uchospitals.edu wrote: From: debra.or...@uchospitals.edu debra.or...@uchospitals.edu Subject: [Histonet] Bone marrow processing on Peloris To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 10:36 AM Good morning, I am currently looking at the possibility of running bone marrow cores and clots on our Peloris. Has anyone had success with this, and if so would you be willing to share your protocol? We use xylene on our runs. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How Have You Been Lately?
Hello Dear, How are you doing these days? You know, I bought one new pair of Nike shoes on one great online shop www.HOYOY.com. They are so amazing! They provide a lot of other brand new casual shoes, sport shoes, high heels, ugg boots, such as Prada, Christian Louboutin, Gucci, Lascote, Puma, and so on. The styles are the most popular ones for the 2010 year. Have to say it is a great and specialize online shoes store. Hope you can find some great things there too www.HOYOY.com.! Give my regards to your family then ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Special Stains for Macrophages
Hi, We are needing to do a special stain for macrophages. What is the most common stain for that? Does anyone do a Sudan Black, Alcian Blue or Van Gieson for macrophages? Any information would be appreciated. Thanks in advance. Sharon This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] How Have You Been Lately?
spam is getting through - Forwarded Message From: Kelly Larson kfine...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Tue, January 26, 2010 11:55:20 AM Subject: [Histonet] How Have You Been Lately? Hello Dear, How are you doing these days? You know, I bought one new pair of Nike shoes on one great online shop www.HOYOY.com. They are so amazing! They provide a lot of other brand new casual shoes, sport shoes, high heels, ugg boots, such as Prada, Christian Louboutin, Gucci, Lascote, Puma, and so on. The styles are the most popular ones for the 2010 year. Have to say it is a great and specialize online shoes store. Hope you can find some great things there too www.HOYOY.com.! Give my regards to your family then ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histology Special Stains for Macrophages
Giemsa will stain them very well. René J. --- On Tue, 1/26/10, Willman, Sharon sharon.will...@bms.com wrote: From: Willman, Sharon sharon.will...@bms.com Subject: [Histonet] Histology Special Stains for Macrophages To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 12:11 PM Hi, We are needing to do a special stain for macrophages. What is the most common stain for that? Does anyone do a Sudan Black, Alcian Blue or Van Gieson for macrophages? Any information would be appreciated. Thanks in advance. Sharon This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslipping
Do any of my Esteemed Colleagues have a photo somewhere of someone hand-coverslipping? I need a picture of a normal hand coverslipping (putting coverslip ON slide instead of slide ON coverslip). Just one photo is all I need. I'm trying to describe in words how to do this and it's not easy! Thanks in advance for your Digital Donation (and I mean electronic digital, so don't give me any flak). If you can email it, I can pass it along. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris and Bond service
We're looking into service agreements for these instruments. Can anyone who currently uses them help me decide which Leica service contract would be best? How often do you experience problems with these instruments that you need to have service for them? What is your volume? Could those service calls have been avoided if you had a/another pm done? Thank you in advance! Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 20% NaOH Nail pre-Treatment... Questions
Hello Joe, first thanks so much for sharing your expertise here on Histonet. I work in the main histo lab at Mass General Hosp. in Boston and I have interested the powers that be in your NaOH protocol. I initially used your NaOH idea to attach an impossible nail to slides by floating 4um section on 20% NaOH for 10-15min. (The transfers between regular water bath and NaOH bath (and back to rinse) were surprisingly easy because the concentrated NaOH prevents adhesion to even the stickiest adhesive slides.) Anyhow, I have some questions for you about your lab's implementation. I found your most complete post at http://lists.utsouthwestern.edu/pipermail/histonet/2008-October/040078.html But I am concerned about the safety of 20% NaOH in routine use. Would you mind posting or emailing me any SOPs you have that address the safety issues? Specifically both the histology lab and the grossing labs here use RDO decal solutions at every bench. So there is/will be concern about the proper handling of 20% NaOH around RDO... by overworked PAs and residents, etc. etc. Feel free to tell me your safety almost-horror stories so we can (try to) prevent them from occuring here. I've seen that some people use 10% NaOH instead. Have you experimented with this? Any reasons you stick with 20% beyond the usual it ain't broke, so...? Have you (or anyone on Histonet) ever tried to find the minimum effective concentration? Increasing the pre-soak time isn't an issue here since I've been assured that nail turnaround times are non-critical. Along these lines, do you or anyone have any histochemical references for the reactions that are taking place? With this info it might be possible to predict the minimum effective pH. It would also be nice to have references for the SOP. (I know about the coverslip crushes some derm clinicians do with fresh scrapings. Alternately, do you know of any histochemical references for this?) Thank you so much for sharing your time and info! And if I happen to answer any of my own questions I will be sure to post what I learn. Sincerely, -brice Massachusetts General Hospital Surgical Pathology, Histology The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Diff-Quik
I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN * On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller cmil...@physlab.com wrote: Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of HE and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 25, 2010 7:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik Thanks, John Kiernan, for your explanation of Romanovsky stains. Diff-Quik (please note the spelling) is the trademarked name of a staining sequence consisting of a fixative, eosin (Diff-Quik I), and an azure (Diff-Quik II), done in that order in three separate containers. I'm not sure who the trademark presently belongs to - it seems to change with the phases of the Moon. There are a number of generic equivalents, which in my personal experience all work as well as trademark Diff-Quik. For most ordinary pathology services, it isn't worthwhile to try to brew your own. I don't think I've seen bone marrow stained with such a sequence. Proper staining of bone marrows requires that the histotechnologist examine the slides under a microscope, a practice too many find abhorrent. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Diff-Quik
Robert, I agree with your comments. There are some labs that never look at their slides (the factories) but there are others (and this number in my experience is increasing) where the technologists often solve the problems before the slides leave the lab. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, 27 January 2010 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN * On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller cmil...@physlab.com wrote: Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of HE and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 25, 2010 7:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik Thanks, John Kiernan, for your explanation of Romanovsky stains. Diff-Quik (please note the spelling) is the trademarked name of a staining sequence consisting of a fixative, eosin (Diff-Quik I), and an azure (Diff-Quik II), done in that order in three separate containers. I'm not sure who the trademark presently belongs to - it seems to change with the phases of the Moon. There are a number of generic equivalents, which in my personal experience all work as well as trademark Diff-Quik. For most ordinary pathology services, it isn't worthwhile to try to brew your own. I don't think I've seen bone marrow stained with such a sequence. Proper staining of bone marrows requires that the histotechnologist examine the slides under a microscope, a practice too many find abhorrent. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] removing trigeminal nerve and ganglion
Why don't you say who and where you are? Someone must have shown you how to take out a rat's brain. This involves cutting the roots of all the cranial nerves and the pituitary stalk. You are then looking at the base of the skull, with the overlying dura mater. The pituitary gland is in the midline, with a white centre (posterior lobe) and dark pink parts laterally (anterior lobe). There is a broad white band on each side, extending caudally from the pituitary. This comprises the trigeminal ganglion, the extradural parts of its roots and the the nerve about to branch into its three divisions. With an acutely pointed scalpel, cut down on either side and below this white object lateral and posterior to the pituitary gland. Remove and fix the excavated object. This is the biggest and easiest sensory ganglion to dissect out of a rat. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Michele Wich mw...@7thwavelabs.com Date: Tuesday, January 26, 2010 11:15 Subject: [Histonet] removing trigeminal nerve and ganglion To: histonet@lists.utsouthwestern.edu Does anyone have experience/a good method for removing trigeminal nerve and ganglion from rat intact? Any advice would be helpful. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping
Is it normal to put the mounting medium on the slide and then apply a coverslip from above? What's wrong with putting the mounting medium on the supine coverslip and then bringing the slide down from above? I prefer the latter method for preparations thinner than 50um. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Breeden, Sara sbree...@nmda.nmsu.edu Date: Tuesday, January 26, 2010 14:13 Subject: [Histonet] Coverslipping To: histonet histonet@lists.utsouthwestern.edu Do any of my Esteemed Colleagues have a photo somewhere of someone hand-coverslipping? I need a picture of a normal hand coverslipping(putting coverslip ON slide instead of slide ON coverslip). Just one photo is all I need. I'm trying to describe in words how to do this and it's not easy! Thanks in advance for your Digital Donation (and I mean electronic digital, so don't give me any flak). If you can email it, I can pass it along. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
