RE: [Histonet] Microwave processing and IHCs
Your control tissue needs to be processed the same as your patient samples. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] inquiry about Arthur Bolles Lee
If you plug his name into google several links appear.. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, March 01, 2010 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inquiry about Arthur Bolles Lee Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator /pre span style=color: rgb(0, 160, 0); font-weight: bold;Please consider the environment before printing this e-mail/spanbr / br / span style=font-size: 8pt;This e-mail, facsimile, or letter and any files or attachments transmitted with it containsbr / information that is confidential and privileged. This information is intended only for the use of the br / individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further br / disclosures are prohibited without proper authorization. If you are not the intended recipient, any br / disclosure, copying, printing, or use of this information is strictly prohibited and possibly a br / violation of federal or state law and regulations. If you have received this information in error, br / please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at br / priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all br / applicable privileges related to this information./spanbr / br / /html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IL-17A
Is anyone using IL17A in mice? where do you buy it?Dilution? where do you buy the secondary? dilution? The protocol ? Thank you so much. Chakib ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 76, Issue 2
Hi Martha, We offer this antibody. Visit our website: www.phenopath.com or call (206) 374-9000 and ask for Client Services for information on specimen submission. Thanks, Stephanie Rodriguez, HTL(ASCP), QIHC IHC Tech III/Clinical Molecular Tech Phenopath Laboratories Seattle, WA On 3/2/10 7:03 AM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Mon, 1 Mar 2010 13:56:17 -0500 From: Martha Ward mw...@wfubmc.edu Subject: [Histonet] IgG4 To: histonet@lists.utsouthwestern.edu Message-ID: 61135f0455d33347b5aae209b903a30433deb...@exchvs2.medctr.ad.wfubmc.edu Content-Type: text/plain; charset=us-ascii Hello all, I am looking for a lab that offers this antibody on ffpe tissues. One of my Pathologists is interested in it and has a surgical case she has been asked to stain for IgG4. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing for HE stains
Good Morning Anyone out there doing HE on cytology slides? Currently I am trying to do HE stains on our FNA's, is there a separate charge for billing the HE per slide? Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 awe...@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing for HE stains
I've never done a HE on cytology just a Pap stain and Diff Quik. The stain charge, however is determined by the preparation technique (a smear or cytospin concentration preparation). The cytospin prep (Non Gyn) is 88108: the smear (NonGyn) is 88104. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net awe...@shorememorial.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/02/2010 10:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Billing for HE stains Good Morning Anyone out there doing HE on cytology slides? Currently I am trying to do HE stains on our FNA's, is there a separate charge for billing the HE per slide? Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 awe...@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sanderson's Bone Stain
Hello anyone out there, A couple of years ago, I ordered Sanderson's Rapid Bone Stain from Surgipath. Since they have gotten bought out, they no longer carry this stain. Does anyone know where I can order it? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ultram fixative
We have a clinic that is fixing tissue in Ultram. I have never heard of it. Can someone educate me about Ultram? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sanderson's Bone Stain
This stain is now available via Dorn and Hart Microedge - www.dornandhart.com Jack From: bustaman...@uthscsa.edu To: histonet@lists.utsouthwestern.edu Date: Tue, 2 Mar 2010 10:01:19 -0600 Subject: [Histonet] Sanderson's Bone Stain Hello anyone out there, A couple of years ago, I ordered Sanderson's Rapid Bone Stain from Surgipath. Since they have gotten bought out, they no longer carry this stain. Does anyone know where I can order it? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcohol followup
As I pressed the SEND button it came to me like a bolt of lightning that alcohol is GRAIN based. Oops... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcohol Source?
I hate to even ADMIT this and I ought to use a disguise when asking - but is alcohol a wood-based solution? I should know this - I know... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Workflow suggestions for MW processing
Thanks to all who have taken the time to reply to my question on microwave tissue processing and the resulting effect (or not) on IHC staining. As you have probably guessed, I am in the process of getting our MWs validated and set up for use. We are still using our conventional processors, but I am very excited about bringing the MWs online. That brings up another issue for me: workflow. We are a M-F lab, pathologists are here from 8-5. They will not be changing their schedules to accommodate the new MWs, so I am left with the task of figuring out the most efficient way to manage my staff, the specimens and the processing. Our basic situation: I currently have 3 histotechs (and myself, if needed on the bench). One comes in at 1am, one at 5am and one at 7am. These shifts can change if needed to make us more efficient. Paths expect slides beginning at 8:30am. The MWs are semi-automatic. They require a human to swap out the cassettes into the next reagent and to press the button to continue the processing. Grossing is done between 10am and 5:30pm. We have two MWs, so we can run smalls and large specimens concurrently. Does anyone have a situation similar to ours? Do you have any suggestions on pitfalls to watch out for? Any suggestions on the best way to achieve both efficiency and good utilization of my staff? THANKS for any thoughts you might like to share! Michelle _ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC
Joe, I wanted to ask you a question about which type of IHC stainer you are using and which stainer do you think is the user friendly and efficient. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, February 26, 2010 4:51 PM To: CHRISTIE GOWAN; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fire in the lab Once upon a time in a far away land, we used to boil our embedding molds in boiling soapy water, over an open Bunsen burner, followed by an alcohol rinse then air dry. One time the fire alarm was activated and we had to evacuate the hospital. We were out there quit awhile. When we received the all clear to go back into the hospital, I was the first one back in the lab and the fire department was there, looking into our pot that had boiled out and was smoking up the lab. This wasn't the cause of the first alarm, but it did set off the second. Joe - Original Message - From: CHRISTIE GOWAN christiego...@msn.com To: histonet@lists.utsouthwestern.edu Sent: Friday, February 26, 2010 8:20 AM Subject: [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
: [Histonet] frozen sections of cartilage
Hi Kim, Try probe on plus slides by fisherbrand for frozen samples. We use it for sectioning frozen tendon. Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguz...@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, March 02, 2010 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: CD31 in rats (Hernandez, Anna) 2. CD 31 on Pig (Reuel Cornelia) 3. Re: CD 31 on Pig (Alexandra Meinl) 4. IgG4 (Martha Ward) 5. RE: Histonet Digest, Vol 76, Issue 1 (Madary, Joseph) 6. Herpes Simplex Virus Types 1 2 for IHC (Sebree Linda A) 7. Job opening (kim.dona...@bhcpns.org) 8. Ab for Cartilage (Tamasi, Joseph) 9. HSV I II (Sebree Linda A) 10. inquiry about Arthur Bolles Lee (LINDA MARGRAF) 11. Microwave processing and IHCs (Green JumpyOne) 12. RE: Microwave processing and IHCs (WILLIAM DESALVO) 13. Re: RE: [Histonet] CD31 in rats (TF) 14. RE: HSV I II (Hoekert, W.E.J.) 15. RE: Microwave processing and IHCs (Mike Pence) 16. Re: Microwave processing and IHCs (Rene J Buesa) 17. RE: Microwave processing and IHCs (McMahon, Loralee A) 18. FW: [Histonet] Microwave processing and IHCs (Vickroy, Jim) 19. RE: inquiry about Arthur Bolles Lee (Horn, Hazel V) -- Message: 1 Date: Mon, 1 Mar 2010 13:01:03 -0500 From: Hernandez, Anna anna.hernan...@crl.com Subject: RE: [Histonet] CD31 in rats To: Annette Featherstone a...@buffalo.edu, histonet@lists.utsouthwestern.edu Message-ID: 63ec5e7c7346eb4ba09d6dc5651e061bea5...@ent-pr-xch-02.na01.crl.com Content-Type: text/plain; charset=us-ascii Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each) Covance blocking reagent (25 min) Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0 ug/mL Covance Labeling Reagent (25 min) Covance Dab (2 x 5 min) (Each step has PBS washes in between and is performed at RT) Thanks, Anna -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Monday, March 01, 2010 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rats Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Mon, 01 Mar 2010 12:10:13 -0600 From: Reuel Cornelia reuel.corne...@tsrh.org Subject: [Histonet] CD 31 on Pig To: histonet@lists.utsouthwestern.edu Message-ID: 4b8baea502c500071...@mail.tsrh.org Content-Type: text/plain; charset=US-ASCII Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you. Reuel -- Message: 3 Date: Mon, 1 Mar 2010 19:37:31 +0100 From: Alexandra Meinl alexandra.me...@gmail.com Subject: Re: [Histonet] CD 31 on Pig To: Reuel Cornelia reuel.corne...@tsrh.org, a...@buffalo.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: b7eff7a1003011037o3f785045l5040524c802f4...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 We use the Rabbit anti-PECAM-1 (sc-1506-R, Santa Cruz) 1:100 on paraffin sections (HIER with citrate buffer pH 6.0 required). It works on pig and rat tissue. Alexandra 2010/3/1 Reuel Cornelia reuel.corne...@tsrh.org Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you. Reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re Billing for HE stains
It is my understanding that CPT 88173 for Dx of FNA covers your stains Diff Quick, HE, PAP you can add 88305 for a cell block but not charge for each stain. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Deleting extraneous parts of posts when replying
Just a friendly reminder that if you are responding to a question to please delete out everything but the pertinent information, especially if you are receiving/responding in Digest mode. If you don't cut out the irrelevant posts it makes it extremely hard to find the new stuff. Some of the Digest versions that have come out in the last few days have Digests within Digests within Digests so it makes the email really long and you can't find the new stuff in amongst the old. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow fixative
Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stainer/coverslippers
Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Deleting extraneous parts of posts when replying
given how many people cant even figure out how to properly unsubscribe from this list, i fear your request might be in vain ;-) Mark Elliott wrote: Just a friendly reminder that if you are responding to a question to please delete out everything but the pertinent information, especially if you are receiving/responding in Digest mode. If you don't cut out the irrelevant posts it makes it extremely hard to find the new stuff. Some of the Digest versions that have come out in the last few days have Digests within Digests within Digests so it makes the email really long and you can't find the new stuff in amongst the old. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoTech Position at DCH Regional in Tuscaloosa, AL
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL. Lab Automation in the lab includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. DCH is a 500 plus bed regional medical center. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
We have the Sakura Prisma and attached film coverslipper. We love both!! Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainer/coverslippers Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
Vicki, We just ordered the Leica combo.We have the separate units of the Autostainer XL and CV5030. They now sell as a combo (a transfer station moves the racks) As we've relied on Leica for years, I expect we will be happy with the new unit. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainer/coverslippers Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stainer/coverslippers
Sakura René J. --- On Tue, 3/2/10, Gauch, Vicki gau...@mail.amc.edu wrote: From: Gauch, Vicki gau...@mail.amc.edu Subject: [Histonet] HE stainer/coverslippers To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] bone marrow fixative
Buffered 10% NBF, but with the pH exactly at 7.0, checked for each case. IHC is perfect. René J. --- On Tue, 3/2/10, Amy Farnan farn...@nehealth.com wrote: From: Amy Farnan farn...@nehealth.com Subject: [Histonet] bone marrow fixative To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:12 PM Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoTech Position At DCH In Tuscaloosa, Alabama (Phone contact correction)
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL. Lab Automation in the lab includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. DCH is a 500 plus bed regional medical center. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5736 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
We have Leica for both and love them.. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainer/coverslippers Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Deleting extraneous parts of posts when replying
Hi, Just wondering if anyone has use hydroethinide to detect the free radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin fixation and parrafin embedding. I wanted to used the hydroethidine on paraffin sections. I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and paraffin embedding plus deparaffination. All the papers I reviewed used hydroethidine on frozen section but our facility does not have vibratome or cryostat. Please help Please Help. Fabrice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bone marrow fixative
We use zinc formalin for the bone marrow specimens, and the pathologists are satisfied with the results. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Amy Farnan Sent: Tuesday, March 02, 2010 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow fixative Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
We have the Leica CV5030 and ST5020 with the transfer station and are very pleased with them. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Bernice Frederick Sent: Tuesday, March 02, 2010 3:35 PM To: 'Gauch, Vicki'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE stainer/coverslippers Vicki, We just ordered the Leica combo.We have the separate units of the Autostainer XL and CV5030. They now sell as a combo (a transfer station moves the racks) As we've relied on Leica for years, I expect we will be happy with the new unit. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainer/coverslippers Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] National Histotechnology Professionals Day
Yesterday Georgia Governor Sonny Perdue signed a proclamation declaring March 10, 2010 the first National Histotechnology Professionals Day in Georgia. Go to www.histosearch.com/gshhttp://www.histosearch.com/gsh to see a great photo of this memorable occasion for the histology professionals of Georgia on our home page. I hope everyone will seize the opportunity to inform our communities of our contribution to their tissue diagnoses and treatment. While you at the website go to the symposium page and register to attend our meeting March 26-27 to be held at the Evergreen Marriott Convention Resort, Stone Mountain, GA. The program, registration form, and a direct link to the hotel with meeting code already entered for your convenience. Just finish the reservation information. Our speakers include James Burchette, Joe Myers, Damien Laudier, Lualhati Harkins, Marvin Hanna, Gina Rodriguez, Kris Fidler, and Jack Ratliff. Make your plans now to attend. Deadline for registration without a late fee is March 15th. Shirley A. Powell, HT(ASCP)HTL, QIHC GSH Secretary Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IF staining on peritoneal macrophages
Hello Histoneters, I am very happy to say that my cytospins are finally working and that cells are staying on the slides throughout the procedure. Thank you very much to all who chimed in or helped with trial samples. I was almost ready to declare victory when I noticed one of my negative control slides looked a bit much like it has specific staining. The antibodies I have been using have not been giving the most even results. Does anyone have a recommendation for an anti-mouse F4/80 antibody? And a matching FITC-conjugated secondary antibody? Is anyone here doing IF staining of mouse peritoneal macrophages? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alcohol Source?
depends. A fine Scotch whiskey may be aged in old oak casks, while a delicate Tequila may not be - Original Message - From: Breeden, Sara sbree...@nmda.nmsu.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Tuesday, March 02, 2010 10:44 AM Subject: [Histonet] Alcohol Source? I hate to even ADMIT this and I ought to use a disguise when asking - but is alcohol a wood-based solution? I should know this - I know... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Ultram fixative
Richard Cartun asks We have a clinic that is fixing tissue in Ultram. I have never heard of it. Can someone educate me about Ultram? I can't Google any evidence for an Ultram fixative. Ultram is a trade name for tramadol, an opioid analgesic. Either somebody is confused, or you've got somebody involved in dubious drug transactions. Can anybody in the clinic shed any light on the matter? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Lab Aid position
Hi everybody; I'm an experienced Lab Aid looking for a job in the Central Florida area (Orlando). I have worked in Histology for over 3 years, and I'm very familiar with all the equipment and the everyday routine of the Lab. I can furnish a full resume on request. I'd appreciate any pointers anyone could offer. Andres Garcia andrespepo82 at yahoo dot com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
