[Histonet] IL17A
Hello Chakib I can recommend this paper: Cutting Edge: Mast Cells Express IL-17A in Rheumatoid Arthritis Synovium. Axel J Hueber, Darren L. Asquith, Ashley M. Miller, Jim Reilly, Shauna Kerr, Jan Leipe, Alirio J. Melendez, Iain B. McInnes. J Immunol. 2010, 184:000-000 ahead of print. Jim Reilly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need help with FISH staining protocol on
Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept of somebody in German, that worked with special plasmid for rat. There are not repeated works in pubmed. I doubt that this method works good for rats or mices, but Cumbio has a kit, maybe it will work. Regards, Julie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Where did Dezna C. Sheehan practice?
Hi All, Dezna Sheehan worked at the Hospital of the University of Penn. for many years. She ran a school for Histotechnology which I and many of my friends and colleagues were lucky to have gone to. We are proud to continue her push for the advancement of our field. Happy Histotechnology Professionals Day!! Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Veronica Coker [veronica...@hotmail.com] Sent: Wednesday, March 10, 2010 6:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where did Dezna C. Sheehan practice? Happy Histotechnology Professionals Day! One of my Professors told me that Sheehan(of Theory and Practice of Histotechnology-by Dezna C. Sheehan, Barbara B. Hrapchak) practiced her work in Philadelphia. I have interest in this for I am originally from Philadelphia. Does anyone know where she did most of her work? Thank You, -Veronica Coker _ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Stories
Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhi...@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: SC Governor Signs Proclamation
The mayor of Kansas City also signed a proclamation stating March 10th is Histotechnology Professional's Day :-) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Wednesday, March 10, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SC Governor Signs Proclamation Good Afternoon Everyone, Vinnie, Chad McMahan and I have just returned from the SC State capital for Governor Sanford to sign a proclamation for Histotechnology Professional's Day. It was a rewarding experience having our Governor acknowledge our profession with the signing of the proclamation. I hope everyone had a GREAT 1st annual HPD!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Excelsior Vs. VIP 6
Hello to the World Wide Histo's, We are looking at getting a new processor and was wondering what everyones feelings were on the Excelsior from Thermo vs the VIP 6 from Sakura. We have a VIP5 right now and it is a delight. The demo of the Excelsior was very impressive. Thanks for your thoughts. Matt HT (ASCP) Longmont United Hospital Longmont, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin vs alcoholic formalin
Help! I think I know the answer, but need some rapid clarification. Is an alcohol-formalin fixative acceptable for use in breast tissue, or does it need to be an aqueous 10% NBF? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histo Stories
Sally, I didn't get a chance to answer yesterday - I went to a high school and spoke about my favorite topic - histotechnology! Anyway, I started out in microbiology and then worked in a doctor's office lab doing routine chemistry and hematology and when we moved to a small town in Iowa the only position in the lab that became available was in histology. I was playing bridge with the histotech and he mentioned the opening so I went in and interviewed, got the job and never have looked back. So I sort of fell into this profession as did many others. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 6:09 AM, Breeden, Sara wrote: Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhi...@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Jobs Alert - Hot New Histology Jobs Histotechs, Managers, PA's
Hi Histonetters!! I hope everyone is having a great day! I wanted to take a moment and tell you about some new opportunities I am working with. Here is a list of my current openings. All of these positions are fulltime permanent positions and my clients offer excellent salaries, benefits and relocation assistance. HISTOLOGY/PATHOLOGY MANAGEMENT AZ - Phoenix Histology Lab Manager VA - Richmond Histology Manager NY - Long Island Histology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor NV - Las Vegas Histology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab TX - Austin Night Shift Grossing Histotech excellent shift diff TX - Corpus Christi - Histotechnician day shift private lab MA - Cape Cod Immunohistochemistry Specialist MA - Cape Cod Histotechnologist/Histotechnician GA - Atlanta area Histotechs needed all shifts GA - Atlanta area Immunohistochemistry Specialist 2p-10p GA- Atlanta Grossing Histotechnologist Night Shift Great Shift diff NY-Orange/Rockland County Brand New Lab NYS license/Elig brand new lab 2nd shift. CA-Los Angeles Histotechnologist afternoon shift NV - Las Vegas Histotechnician/Histotechnologist PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histo Stories
One more story!! From 2 yrs of age I had wanted to be a nurse. Love/marriage got in the way and I didn't do the nursing program but started out for a degree in Biology. Then dropped out of school and obtained PhT (putting hubby through). Applied at the Youth Opportunity Center in Knoxville, TN in the fall of 1966. Received a call asking if I would be interested in training in Histology. I had not a clue, but said yes. Trained at UT Hospital and was registered in 1968. Took enough classes here and there from TN to Alaska to NM to have enough requirements to sit for HTL and obtained my degree in Business Management from Tusculum College Adult Studies. May Momma was so proud...only took me 30 years to finish my degree! :) And here I am...still at it. Love this site and feel so connected to you all through it. Very grateful to Linda and Herb for their foresight to originate it and their patience with us. Thanks for your vast wisdom. I am amazed at what some of you do and wish I had time to read in detail and learn it all. I would like to sit at your feet and soak it all up!! Blessings to everyone, Joyce Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Stories
I am really enjoying reading these start-up stories. :o) If you don't mind indulging me, I'll share mine too! I had just graduated from college with a BS in Biology when I landed a job as a cancer research assistant. Well, it turns out that portion of lab just wasn't for me! I left that department and worked in another area of the lab. Then the histotech quit. They looked at me, said you have a degree, you can do this. I had no idea what histology was other than looking at the cells under the microscope! They had no problem with that and they set about teaching me how to use the equipment. I had no theory, no understanding of *why* I was doing any of what I was doing, I just learned the practical side histology: process, embed, cut, stain. I learned how to cut with my knees in a cupboard (they didn't have a proper desk for me) and not with forceps or brushes, but with chop sticks! You see, the first person to introduce me to Histology was a graduate student, from Japan, who was doing an internship at our facility. :o) I stayed at that position for about 1.5 years, but absolutely had to leave it because I developed a very severe allergy and asthma to the rats we were doing our research on. I was offered a position in (what was then) the largest private lab in MI. My true mentor, Glenda, taught me anything and everything I know about Histology. She helped me study for the HT exam, spending countless hours of her own time helping me learn. Thanks to her, I passed the HT the first time around! Later, she assisted me in studying for the HTL exam which I also passed! Had it not been for her kindness and guidance, I'm not so sure I would have succeeded. :o) THANKS GLENDA!! Glenda had no formal education after high school - everything she learned was via on the job training. I will say, she learned very, very well! :o) So much so that she now has a QIHC after her name. And now where are we? I am trying to figure out just how to have our very own Histotech school here at my hospital. We are affiliated with another school (with me as the mentor), but I am thinking it would be nice to run our own. See? What goes around, comes around! It's amazing just how far we have come! From stropping our own knifes, to disposible ones; from maintaining our (and in some cases, making new parts!) microtomes to having maintenance free ones; from all the manual staining to the automated; and now microwave technology for the processing. Yes, indeedy, we sure have evolved! Gone are the days of grabbing someone from the lab and saying you can be a histotech!! We have to be formally educated now! :o) I love my job! Michelle _ Hotmail: Trusted email with Microsoft’s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laura Miller is Out of the Office.
I will be out of the office starting 03/11/2010 and will not return until 04/05/2010. I am on medical leave until April 5. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology Stories
Makes my story kind of dull! I was in college in nursing then went to Allied Health (MT, more or less) but had no idea histo existed until I got to this point. I thought it was more interesting than running blood and urine samples and went for it. Histo school and then the HTL. That was 26 years ago (eek). I worked most of my years in a hospital setting, now I work in a core lab and we do animal translational studies for researchers here at NU as well as being the reference lab for ECOG, who are one of the biggest cooperative groups in the country running cancer clinical trials. We get all the blocks and or slides for these trials here at NU from the US as well as other countries. Makes for a varied and interesting job. And yes, I do remember the days of regular knives and making up my own Schiff's! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Thursday, March 11, 2010 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I am really enjoying reading these start-up stories. :o) If you don't mind indulging me, I'll share mine too! I had just graduated from college with a BS in Biology when I landed a job as a cancer research assistant. Well, it turns out that portion of lab just wasn't for me! I left that department and worked in another area of the lab. Then the histotech quit. They looked at me, said you have a degree, you can do this. I had no idea what histology was other than looking at the cells under the microscope! They had no problem with that and they set about teaching me how to use the equipment. I had no theory, no understanding of *why* I was doing any of what I was doing, I just learned the practical side histology: process, embed, cut, stain. I learned how to cut with my knees in a cupboard (they didn't have a proper desk for me) and not with forceps or brushes, but with chop sticks! You see, the first person to introduce me to Histology was a graduate student, from Japan, who was doing an internship at our facility. :o) I stayed at that position for about 1.5 years, but absolutely had to leave it because I developed a very severe allergy and asthma to the rats we were doing our research on. I was offered a position in (what was then) the largest private lab in MI. My true mentor, Glenda, taught me anything and everything I know about Histology. She helped me study for the HT exam, spending countless hours of her own time helping me learn. Thanks to her, I passed the HT the first time around! Later, she assisted me in studying for the HTL exam which I also passed! Had it not been for her kindness and guidance, I'm not so sure I would have succeeded. :o) THANKS GLENDA!! Glenda had no formal education after high school - everything she learned was via on the job training. I will say, she learned very, very well! :o) So much so that she now has a QIHC after her name. And now where are we? I am trying to figure out just how to have our very own Histotech school here at my hospital. We are affiliated with another school (with me as the mentor), but I am thinking it would be nice to run our own. See? What goes around, comes around! It's amazing just how far we have come! From stropping our own knifes, to disposible ones; from maintaining our (and in some cases, making new parts!) microtomes to having maintenance free ones; from all the manual staining to the automated; and now microwave technology for the processing. Yes, indeedy, we sure have evolved! Gone are the days of grabbing someone from the lab and saying you can be a histotech!! We have to be formally educated now! :o) I love my job! Michelle _ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori on Rhesus macaque tissue
Dear List servers, Did anyone try this antibody on Rhesus macaque tissue? Do you have a protocol? How were the results? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Stories
I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a family of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say Happy Histologist Professional Day! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histo Stories
Sally, I started in Electron Microscopy after taking a two-year EM course at Delta College in Stockton, CA. I had never seen a histology lab before starting work at Valley Medical Center in Fresno, CA. I gradually started helping out in histology when I didn't have enough EM work to keep me busy. I started by coverslipping, worked into special stains, then cutting (though I never really cut very much). I started the immunohistochemistry service there in 1983. The guys in our lab (literally - we had four men, no women!) started a study group to work on the HT test and we took that in 1989, all passing. Later I took the HTL and passed that. After I left Valley Medical center ion 1993 I worked primarily in immunohhistochemisty and managed histology labs. I went to Saudi Arabia for 5 years, then Centers for Disease Control in Atlanta, into industry with Lab Vision (IHC Autostainer and reagents - a very eye-opening education on the vendor side of things!), and now back to histology at University of California, San Francisco Medical Center. Histology is so varied and hands-on that it is always interesting. There is always something new coming along. I started in histology just before the IHC revolution, when virtually everything done in histology was based on 50 to 100-year-old technology. Now we are at the forefront right along with every other lab discipline. It has been a good ride all the way. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, March 11, 2010 5:09 AM To: histonet Subject: [Histonet] Histo Stories Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhi...@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histology Stories
I was introduced the color world of Histology, when I was about 7 or 8. That reminds me of when I first brought my daughter to the lab. She was about 6 and one of our pathologists was there. He asked if she wanted to see anything and she piped right up I want to see some brains! So he took a whole brain out of a bucket and she was kind of speechless as she looked at it. Finally she says It doesn't look like spaghetti at all! Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Thursday, March 11, 2010 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a family of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say Happy Histologist Professional Day! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histology stories
Another funny story: When my son was in kindergarten, the teacher went around the class asking the kids what their parents did. When she got to Michael he stood up and announced quite boldly she cuts up brains!!. The teacher asked me at the next conference what I really did and after explaining histology to her, she told me the story and said that she was afraid to ask him for more details on that day! I got a chuckle out of that and so did she! LuAnn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histology Stories
Your introduction into histology brought back a very funny memory for me. My kids are in their 30's now, but when my daughter was 7 or 8 she also saw her first grossing specimen which was also a leg. She tried to talk about it in show and tell and her teacher made her stop. She didn't want to hear about it and my daughter was so excited about being able to share the information with her classmates. When my son was 14 or 15 he wanted to see a brain and we happened to have one from an autopsy case. I took it out and he got one whiff of the formalin and said Mom, no wonder you are so weird, having to smell that stuff everyday. No one can appreciate what we do quite like our children!! Jessica, that was a really cool story about your Mom introducing you to histology. It brought a tear to my eye! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Thursday, March 11, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a family of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say Happy Histologist Professional Day! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used equipment
We're doing some renovating in our Histology department (YEAH!) and have some equipment that we no longer need. If no vendors are interested, we would consider donating them. Labconco Protector Laboratory Hood (47Wx59Hx31D) Tissue Tek II cryostat Leica TP1050 processor MT920 microtome All equipment has had regular pm's. Anyone interested may contact me off-line. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
http://www.globo.tur.br/bLGiY6ZPO0.html _ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Excelsior Vs VIP 6
Hey Histo gang, We are looking for opinions not sales calls. So please do not pass this onto vendors and give us your opinon so we have good feed back from our peers. Thanks, Matt HT ASCP Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Story
Hello All, I had never heard the before starting as a Histotech trainee in Tulsa, OK. The funny story I wanted to share was similar to some others submitted. My oldest son, at five, was asked by his teacher, What does your father do? He replied, He cuts up dead people. Much to my dismay at the next parent teacher conference I was asked by the teacher what I did for a living and my reply was I am a Histotechnician and a Diener. She then asked, as most people do, What is that?. I began to explain the histology part and that I did autopsies. Then she explained what my son had told her earlier. Knowing that I had not ever told my children that I performed autopsies it came as quite a shock. So I asked my wife if she had even mentioned this to the kids and the answer was of course no. It turns out the babysitter had told them. Needless to say she no longer watches our children. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Excelsior Vs. VIP 6
We just purchased a VIP 6 in January. It is wonderful - user friendly, many programming options and Tissue-Tek's reliability with its tissue processors make it the best processor (in my opinion) being sold today. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Histo story
- Forwarded Message From: Rena Fail renaf...@bellsouth.net To: nmhi...@comcast.net Sent: Thu, March 11, 2010 1:48:12 PM Subject: Histo story A single parent at 29 with 4 children, unpredictable child support, and only a high school education, I could only get minimum wage jobs. I started looking into federally funded educational programs. the obvious, becoming a nurse, but that program had met its quota. I wanted to work in the health field so the adviser started going through the college catalog to see what other 1-2 year programs were offered in the health field. When we read the very short but descriptive paragraph for histology I knew that was it, that was what I wanted to do, I couldn't wait. So it was back to school at 31 and for 30 years I practiced the Art of Histology. From that short paragraph to a career in a profession I loved and for which I never loss enthusiasm or wonder. For nearly 20 years I worked for the department head Gordon R. Hennigar who taught me to trust my instincts and always always add to my knowledge, read the old books and publications as well as the new. To do the very best work every day so that the pathologist could do his best for the patient. I was also privileged to have worked in the same department with Drs. Joseph McManus and Sam Spicer. Both of these researchers would not hesitate to answer a techs questions. Though Sam Spicer usually responded with copies of articles he had written and a mini lecture. The last years before my retirement my immediate boss was Vinnie who taught me to put pen to paper to share with others. i learned to write manuals, develop stain procedures, play with dyes, put out fires, help pathologists, work with residents and help patients, all because of one little paragraph in a college catalog. Rena Fail Retired from Medical University o fsouth Carolina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] operator manual
I am in need of an operators manual for the Leica 2025 microtome , Leica 2040 microtome and the Hacker 3660 Glass coverslipper . If anyone has any of these could you please sent it to me as pdf file, I would greatly appreciate it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histo Story
That's a funny one. My son told his teacher and classroom that his mom cut up dead people and made slides out of them. It was a long time living that one down. He understood exactly what he meant but the rest of his class could only relate to the equipment on the playground! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matt Brooks Sent: Thursday, March 11, 2010 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histo Story Hello All, I had never heard the before starting as a Histotech trainee in Tulsa, OK. The funny story I wanted to share was similar to some others submitted. My oldest son, at five, was asked by his teacher, What does your father do? He replied, He cuts up dead people. Much to my dismay at the next parent teacher conference I was asked by the teacher what I did for a living and my reply was I am a Histotechnician and a Diener. She then asked, as most people do, What is that?. I began to explain the histology part and that I did autopsies. Then she explained what my son had told her earlier. Knowing that I had not ever told my children that I performed autopsies it came as quite a shock. So I asked my wife if she had even mentioned this to the kids and the answer was of course no. It turns out the babysitter had told them. Needless to say she no longer watches our children. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] One more story to add
Back in 1974, I wanted to take some summer classes through my high school. There was an interesting class through the Ingham Intermediate School District called Exploratory Health. It highlighted three vocational programs at the Career Center in Mason, Michigan, one of which was called Histology. I spent a week being exposed to Dan Spencer, then instructor showing us what the program and Histology was all about. I was hooked and at the tender age of 14, started the process of being admitted to the program. I started in the fall of 1976 in this ASCP accredited program under the new teacher Betsy Krummery. Betsy was not a whole lot older than we were, but she was a great teacher. I went half day to the program for two years, concluding with three internships at two area hospitals and one in the Department of Human Medicine's Pathology Department at MSU. There I met one of the most influential Histotechnologists that I have ever worked with - Nina Miller. By graduation in 1978 I was board eligible, and applied at the next available time which was August. As us old timers recall, you could only apply in February and August, and then took the practical, followed by the written which I passed the 1st time and have been a registered tech for the last 31 years. Mary (Quandt) Abosso Aurora, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histology Stories, Part 3
You have book in the making! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, March 11, 2010 2:05 PM To: histonet Subject: [Histonet] Histology Stories, Part 3 I am stunned and amazed at the amount of response this little query has gotten! I have forwarded those that were posted here to my home email (and I've gotten many there, too) and expect that I'll get many, many more. I know not everyone has time to respond to email every single day, so I'm giving it more time. Unless you specifically request to remain anonymous, I'm going to give each contributor credit for their story. And because we have so many members from around the world, I would very much like to know how our fellow techs got started in histology in their countries. But this whole endeavor has been very interesting and I thank each of you for sharing your story. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Stories, Part 3
I am stunned and amazed at the amount of response this little query has gotten! I have forwarded those that were posted here to my home email (and I've gotten many there, too) and expect that I'll get many, many more. I know not everyone has time to respond to email every single day, so I'm giving it more time. Unless you specifically request to remain anonymous, I'm going to give each contributor credit for their story. And because we have so many members from around the world, I would very much like to know how our fellow techs got started in histology in their countries. But this whole endeavor has been very interesting and I thank each of you for sharing your story. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] television coverage of Histotechnology Professionals Day
I thought some of you might be interested to learn that our histo lab staff were interviewed yesterday by the Charleston CBS affiliate station Live 5 News. The interview was telecast last evening at about 5:30 pm so we succeeded in getting histology on the air waves. The news reporter showed up with almost no warning while I was enroute to the state capital with Wanda Smith to meet with our governor. I'm thrilled that our techs were in the spotlight. They and all of you, deserve to be recognized for all that you do to enhance our quality of life. If you'd like to see the video clip, go to http://www.live5news.com/Global/story.asp?S=12118805 on that page, off to the right, you will see the phrase Lab techs recognized for work behind the scenes Click on that phrase and the video will open. Turn up your speakers as the volume is a bit inconsistent. The telecast was perfect in every way. The video clip does not include two teasers that preceded the actual segment, you know the ones where they say when we return, histotechs at MUSC... Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histo Stories
I began working in a lab in Northern Kentucky right out of high school as a Diener, washing glassware and assisting with autopsies. During the slow times I would hang out in Histology, watching the one tech in the building work her magic. After a year or so the lab director decided to add another tech and they agreed to train me. After a few years of OJT I sat for the exam and passed it with flying colors. I continued my education, eventually getting a PhD, but I am so glad I stumbled into the Histology field. I, too, had no idea what Histotechnology was when I was in high school, but in the edited words of Garret Morris Histology been berry, berry good to me! Mark Turner, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, March 11, 2010 7:59 AM To: Breeden, Sara Cc: histonet Subject: Re: [Histonet] Histo Stories Sally, I didn't get a chance to answer yesterday - I went to a high school and spoke about my favorite topic - histotechnology! Anyway, I started out in microbiology and then worked in a doctor's office lab doing routine chemistry and hematology and when we moved to a small town in Iowa the only position in the lab that became available was in histology. I was playing bridge with the histotech and he mentioned the opening so I went in and interviewed, got the job and never have looked back. So I sort of fell into this profession as did many others. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 6:09 AM, Breeden, Sara wrote: Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhi...@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC worksheet reports
I was wondering if anyone could give me some information on the IHC worksheet reports. These are the reports that are printed off after you have completed your IHC stain. We are using a Dako Autostainer + and an IntelliPath, we give our Pathologist the reports with the slides to sign off on the stain. Does anyone know how long you need to keep these worksheet reports? Thank you, Andrea O'Brien HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histo stories
I started out as a Med. Tech student. I changed my mind when I was working as a Phlebotomist in a Wisconsin Hospital. In the lab was a small room with 2 techs in it. Through the window I could see tissue on the counter. Curiosity won out. I soon started Histology training also at Marshfield, WI. And the rest is history. My mom often tells me that people around my home town ask her how my job in the history department is going! Sharon Campbell Sharon Campbell HT, HTL (ASCP) Histology Supervisor Celligent Diagnostics, LLC 106 Venture Blvd. Spartanburg, SC 29306 (864) 583-3850 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC worksheet reports
We keep ours for two years. Why I do not know. CAP says to keep the requisitions two years so we followed this rule. BUT why is a mystery - some of the pathologist mark OK on them or repeat. But most of the time they are ignored or shredded by the pathologist. So using them as QA is almost impossible, since sometimes you get them other times you don't. One of the pathologist had about 6 months of these forms in his office that he dropped off one day. How do I file these and find them ever? I am investigating this. And I will ask our NYS inspector when they come in. The CAP inspector said that we did even need them. Just the requisition was needed to track what was ordered versus what was done. The files are stored on the computer indefinitely on both the Dako and the Ventana. Basically my techs just use them to make sure that the correct doctor gets the slides. If you have any ideas please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of aobrie...@comcast.net [aobrie...@comcast.net] Sent: Thursday, March 11, 2010 2:25 PM To: histonet Subject: [Histonet] IHC worksheet reports I was wondering if anyone could give me some information on the IHC worksheet reports. These are the reports that are printed off after you have completed your IHC stain. We are using a Dako Autostainer + and an IntelliPath, we give our Pathologist the reports with the slides to sign off on the stain. Does anyone know how long you need to keep these worksheet reports? Thank you, Andrea O'Brien HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC worksheet reports
We keep them two years like we do other QC data. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of aobrie...@comcast.net Sent: Thursday, March 11, 2010 14:25 To: histonet Subject: [Histonet] IHC worksheet reports I was wondering if anyone could give me some information on the IHC worksheet reports. These are the reports that are printed off after you have completed your IHC stain. We are using a Dako Autostainer + and an IntelliPath, we give our Pathologist the reports with the slides to sign off on the stain. Does anyone know how long you need to keep these worksheet reports? Thank you, Andrea O'Brien HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC worksheet reports
We use them as QC and our pathologists make notes or confirm ok is why we keep them 2 yrs as CAP dictates. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, March 11, 2010 14:37 To: aobrie...@comcast.net; histonet Subject: RE: [Histonet] IHC worksheet reports We keep ours for two years. Why I do not know. CAP says to keep the requisitions two years so we followed this rule. BUT why is a mystery - some of the pathologist mark OK on them or repeat. But most of the time they are ignored or shredded by the pathologist. So using them as QA is almost impossible, since sometimes you get them other times you don't. One of the pathologist had about 6 months of these forms in his office that he dropped off one day. How do I file these and find them ever? I am investigating this. And I will ask our NYS inspector when they come in. The CAP inspector said that we did even need them. Just the requisition was needed to track what was ordered versus what was done. The files are stored on the computer indefinitely on both the Dako and the Ventana. Basically my techs just use them to make sure that the correct doctor gets the slides. If you have any ideas please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of aobrie...@comcast.net [aobrie...@comcast.net] Sent: Thursday, March 11, 2010 2:25 PM To: histonet Subject: [Histonet] IHC worksheet reports I was wondering if anyone could give me some information on the IHC worksheet reports. These are the reports that are printed off after you have completed your IHC stain. We are using a Dako Autostainer + and an IntelliPath, we give our Pathologist the reports with the slides to sign off on the stain. Does anyone know how long you need to keep these worksheet reports? Thank you, Andrea O'Brien HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: television coverage of Histotechnology Professionals Day
Vinnie, Thanks for everything you have done to make the first Histotechnology Professionals Day happen. What a success on many fronts! Jan Mahoney Omaha NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] television coverage of Histotechnology Professionals Day
That is really exciting, Vinnie! Thanks for all you do, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, March 11, 2010 14:15 To: Histonet Subject: [Histonet] television coverage of Histotechnology Professionals Day I thought some of you might be interested to learn that our histo lab staff were interviewed yesterday by the Charleston CBS affiliate station Live 5 News. The interview was telecast last evening at about 5:30 pm so we succeeded in getting histology on the air waves. The news reporter showed up with almost no warning while I was enroute to the state capital with Wanda Smith to meet with our governor. I'm thrilled that our techs were in the spotlight. They and all of you, deserve to be recognized for all that you do to enhance our quality of life. If you'd like to see the video clip, go to http://www.live5news.com/Global/story.asp?S=12118805 on that page, off to the right, you will see the phrase Lab techs recognized for work behind the scenes Click on that phrase and the video will open. Turn up your speakers as the volume is a bit inconsistent. The telecast was perfect in every way. The video clip does not include two teasers that preceded the actual segment, you know the ones where they say when we return, histotechs at MUSC... Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Excelsior Vs. VIP 6
Matt We have both the VIP 6(new)and the Excelsior(older). The Excelsior is a little less user friendly but is a workhorse if maintained properly. There is less solution exposure since you do not have to fill the containers as you do in the VIP6. Both machines produce excellent tissue blocks. The VIP6 has many safe guards so there is little chance for mistakes. With every step in the VIP6 it asks for verification of the command. If you have any more question I can answer feel free to contact me. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com 716-250-9235 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Thursday, March 11, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Excelsior Vs. VIP 6 Hello to the World Wide Histo's, We are looking at getting a new processor and was wondering what everyones feelings were on the Excelsior from Thermo vs the VIP 6 from Sakura. We have a VIP5 right now and it is a delight. The demo of the Excelsior was very impressive. Thanks for your thoughts. Matt HT (ASCP) Longmont United Hospital Longmont, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Formalin vs alcoholic formalin
William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska asks: Help! I think I know the answer, but need some rapid clarification. Is an alcohol-formalin fixative acceptable for use in breast tissue, or does it need to be an aqueous 10% NBF? According to the FDA, the required fixation for the HER2 immunostain on breast tissue is neutral buffered formalin - accept no substitutes! I agree with them. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Darlene G Jones
I would like Darlene Jones to contact me. I have an article she published in the Lab Leader(Shandon-Lipshaw newsletter)in 1996. WOuld love to republish it in our state newsletter. If you know Darlene, please tell her to contact me. Thank you, Marilyn McDonald, HT(ASCP) Little Rock, AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Histology Stories
This story reminds me of the time I had to run into the lab over a weekend and I had my son with me. He was about 6 or 7 at the time. He tripped over a bucket containing a brain suspended for fixing in formalin from an autopsy that was just done and as I was cautioning him to be more careful he asked, what kind of surgery did that person have? Needless to say our dinnertime conversations were very interesting. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 10:05 AM, Beckham, Sharon wrote: Your introduction into histology brought back a very funny memory for me. My kids are in their 30's now, but when my daughter was 7 or 8 she also saw her first grossing specimen which was also a leg. She tried to talk about it in show and tell and her teacher made her stop. She didn't want to hear about it and my daughter was so excited about being able to share the information with her classmates. When my son was 14 or 15 he wanted to see a brain and we happened to have one from an autopsy case. I took it out and he got one whiff of the formalin and said Mom, no wonder you are so weird, having to smell that stuff everyday. No one can appreciate what we do quite like our children!! Jessica, that was a really cool story about your Mom introducing you to histology. It brought a tear to my eye! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu ] On Behalf Of jessica.va...@hcahealthcare.com Sent: Thursday, March 11, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a family of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say Happy Histologist Professional Day! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori on Rhesus macaque tissue
Hi, I am sure this will work. Granted I haven't tried it... The antibody is directed against the bug, the H. pylori bacteria, not against a product of the human it exists in. So it shouldn't matter where the bug is, it should have the epitope the antibody binds with. I do Hepatitis B on non human tissue all the time and it works fine. Good luck, (and let me know for sure how it goes) Amos Brooks Message: 9 Date: Thu, 11 Mar 2010 11:25:16 -0500 From: Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov Subject: [Histonet] H. pylori on Rhesus macaque tissue To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: a7dde3764cfed64984b33e0373eca56b025336b...@nihmlbx09.nih.gov Content-Type: text/plain; charset=us-ascii Dear List servers, Did anyone try this antibody on Rhesus macaque tissue? Do you have a protocol? How were the results? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Openings in Plano, TX
We currently have 2 openings for our private lab in Plano, TX: Benefits include fully paid medical, dental, life insurance, PTO time, generous retirement package. One Full Time Histotech. Must be ASCP registered HT or HTL, or eligible (must become registered within 10 months of hire date). Duties include embedding, microtomy, IHC, special stains. One Full Time Flow Cytometry Tech needed for start up operation. The individual will be responsible for the clinical flow cytometry testing, to include assay validation, implementation, and testing. The scope includes sample preparation, flow cytometric acquisition and analysis, as well as documenting quality control and coordinating quality management for the flow cytometry section. Current experience with assay optimization, validation and antibody cocktailing; current experience with flow cytometry, specifically hematological malignancies; knowledge of CAP requirements for flow cytometry testing; writing and updating laboratory policies and procedures. BS degree in a relevant science with certification in medical technology, histotechnology or cytotechnology required. Interested candidates please contact Genie Jacobs at 972-981-3108 or fax resume to 972-981-3236. The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy Histo Day
No celebration here in Bay Shore. So sad. My story is a long one, some of you might find it interesting. It was 10 years ago that Vinnie encouraged me to put my story to pen and paper and suggested also that we republish my Movat pentachrome modification from the 1972 Histo Logic. The link is here, just scroll down to Memoirs of a Self Made Histotech. (Parts one and two :-) A lot of water under the bridge since that was finished. http://www.sakura-americas.com/histologic/topics/history.html Best wished and Happy Belated Holiday to all my colleagues out there. And, if anyone reading in Europe has any idea how I might find a situation over there, please let me know. I'm itching for something different. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant, Histology Supervisor, Laboratory Safety Officer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recyclers
Does anyone know of any companies out there that buy used recyclers and refurbish them for re-sale? I have a xylene recycler (10 yrs old), alcohol recycler (5 yrs old) and a formalin recycler (3 yrs old) all of which work fine. They are all CBG products. Thanks ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Red Chromogen for MITF antibody
Hi, Does anyone do MITF immunostains with a Red Chromogen. We are using a Dako autostainer, and in the past have used AEC chromogen on MITF. Our pathologist doesn't like it, because it doesn't get red enough. We would like something we could use with current protocols on the Dako, but can't find anything. Any suggestions? Gareth Blaeuer Davis, HT, BS. Pathology Associates of St. Thomas Nashville, Tn 37205 615-298-4100 _ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Robert D Kobus is out of the Laboratory.
I will be out of the office starting Thu 03/11/2010 and will not return until Mon 03/15/2010. I will be out of the Laboratory from Friday 03/12/2010 until 3/15/2010. If you have a matter that can not wait, please page me at 202-801-4851. Otherwise I will return your e-mail on Monday! CONFIDENTIAL: The information contained in this communication, including its attachments may contain confidential information and is intended only for the individual (s) or entity (ies) to whom it is addressed . The information contained in this communication may also be protected by legal privilege , federal law or other applicable law. If you are not the intended recipient of this communication , you are hereby notified that any distribution, dissemination or duplication of this communication is strictly prohibited. If you have received this communication in error please immediately delete and destroy all copies of this message and please immediately notify us of the error by separate communication . Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need help with FISH staining protocol on
Dear Julie, Thanks for the information! I saw some papers about that staining on mice tissues, such as http://www.pnas.org/content/104/10/4030.abstract . I have been talking with Ms Leiker at Univ. of Bufflo, sorry I forgot to click reply to all but reply. She gave me some good suggestions and I am going to try the staining again next week, will let you know if it works out or if I need more help. Thanks! Regards, Jinhui From: J C [jcb...@gmail.com] Sent: Thursday, March 11, 2010 5:36 AM To: Shen, Jinhui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need help with FISH staining protocol on Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept of somebody in German, that worked with special plasmid for rat. There are not repeated works in pubmed. I doubt that this method works good for rats or mices, but Cumbio has a kit, maybe it will work. Regards, Julie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
