[Histonet] Mysterious artifact on GI biopsies HE stain
Hi, We have been getting a similar staining artifact on some GI biopsies randomly while the rest GI biopsies look fine. Also on the affected GI biopsies there is often only 1 or 2 spots of the biopsy that are affected while the rest of the biopsy looks fine. The artifact is being reproduced on each cut; also the artifact doesn't disappear after the affected biopsies get reprocessed. The artifact can be described as a foggy, smudgy stain, with deformed nucleus and not enough cell details. All our specimens are fixed in 10%NBF. Please let me know if you have any ideas. Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraformaldayhde
In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mysterious artifact on GI biopsies HE stain
I would check to see what medication the patient was on or if something was done during the procedure to affect the biopsies.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 07:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies HE stain Hi, We have been getting a similar staining artifact on some GI biopsies randomly while the rest GI biopsies look fine. Also on the affected GI biopsies there is often only 1 or 2 spots of the biopsy that are affected while the rest of the biopsy looks fine. The artifact is being reproduced on each cut; also the artifact doesn't disappear after the affected biopsies get reprocessed. The artifact can be described as a foggy, smudgy stain, with deformed nucleus and not enough cell details. All our specimens are fixed in 10%NBF. Please let me know if you have any ideas. Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti 8 hydroxyguanosine
Hey guys was wondering if any of you used the anti 8 hydroxyguanosine to detect free radical induced DNA and RNa damage. which antibody is the best. we tried the one from abdserotec and it is a disaster. the background is just horrible. any idea ? 2010/4/26 Perry, Margaret margaret.pe...@sdstate.edu In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mysterious artifact on GI biopsies HE stain
It could be a hot biopsy? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 6:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies HE stain Hi, We have been getting a similar staining artifact on some GI biopsies randomly while the rest GI biopsies look fine. Also on the affected GI biopsies there is often only 1 or 2 spots of the biopsy that are affected while the rest of the biopsy looks fine. The artifact is being reproduced on each cut; also the artifact doesn't disappear after the affected biopsies get reprocessed. The artifact can be described as a foggy, smudgy stain, with deformed nucleus and not enough cell details. All our specimens are fixed in 10%NBF. Please let me know if you have any ideas. Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any Histology openings in San Diego area?
Hi Netters, I have a friend looking to relocate to San Diego area and is looking for a Histology position, she is certified HT. Any help would be greatly appreciated, thanks in advance!! Jill Jill Cox HT (ASCP) Arizona Dermatology 4232 E Cactus Rd Phoenix AZ 85032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Two antibodies from the same host with tyramide
Adam, Thanks for the detailed protocol. I can't explain it either but I'll throw some ideas out there and maybe it'll stimulate discussion. Hopefully Chris vanderLoos will chime in. Have you tried doing the labeling in reverse, using the VE-Cadherin first and then blocking with the goat IgG, and then doing the other antibody/tyramide protocol? I'm not sure what effect, if any, the hydrogen peroxide might have on the anti-goat fluorescent 649 label, so you could certainly do that block prior to doing the first primary antibody incubation. Also, you may have to double the concentration of the Cadherin to get it to label at the same intensity as you do in single staining. But still I might have expected to see a signal. Do the two antibodies co-express? Could there be something with the covalent binding of the HRP-tyramide complex that shelters the antigenic sites for the second antibody? That's why I wondered what might happen if you reversed your protocol. Let us know if you get it figured out and what fixed it for you. Good luck, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I highly recommend the Milestone microwave tissue processors. I have used their microwave tissues processors in two different locations and have found them to be very reliable, user friendly, reproducible and cost effective. They also have wonderful tech support. Kaye Ryan Histology Supervisor North Florida Dermatology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 11:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
It really depends on your volume to be processed and what you are planning to process. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 10:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I would look at this very carefully, especially in light of the new regulation that were just released by the CAP on ER and PR. But that is up to each individual lab Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: jel...@yumaregional.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 8:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?
This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out --Hadi or Rich I know they only list two PR clones one is Dako 1294-what is the other 312? Deb From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of ancillaryp...@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Taylor, Jean jtay...@meriter.com 4/23/2010 11:17 AM I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?
Did I understand correctly from Dr. Hammond in Florida that the ER ASCO/CAP
guidelines extended the fixation time to 72 hours? Are they changing the
Her2 guidelines to match the ER? If so, has that happened yet? I have
people very anxious to stop having techs work on the weekends to comply with
the 48 hour fixation limits.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
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From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of
Van Eyck, Deb
Sent: Monday, April 26, 2010 10:30 AM
To: ancillaryp...@mac.com; ihcrg Group (E-mail); histonet netserver
Subject: RE: [IHCRG] ER clone 1D5 or SP1 ?
This is a great discussion lets also talk about PR clones since the ASCO/CAP
guidelines just came out --Hadi or Rich I know they only list two PR
clones one is Dako 1294-what is the other 312? Deb
_
From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of
ancillaryp...@mac.com
Sent: Sunday, April 25, 2010 7:36 PM
To: ihcrg Group (E-mail); histonet netserver
Subject: Re: [IHCRG] ER clone 1D5 or SP1 ?
When we started our lab 3 years ago, we began with SP1 from day 1, so I
don't have any experience with either 1D5 or 6F11 except in my previous
labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in
the work-up of metastatic carcinoma of unknown primary site, based on the
published literature. The advantage of 6F11 is that, for those of us who use
the Allred scoring system, it's the only clone that was clinically validated
by Harvey et al. (JCO 1999) for this purpose. I agree with Rich.
For those who use SP1, it's a very good clone as a predictive marker in
breast cancer. But again, in the setting of metastatic workup, it is NOT
recommended, as it will pick up too many primary lung cancers and some colon
cancers (personal experience).
Hadi
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
President,
Ancillary Pathways
7000 62nd Avenue, PH-C
Miami, FL 33143
T 305-740-4440
F. 786-513-0175
www.vitromolecular.com
www.ancillarypath.com
On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote:
I have looked at several clones over the years and I prefer clone 6F11.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
Taylor, Jean jtay...@meriter.com 4/23/2010 11:17 AM
I'm wondering which clone of ER most labs are using?
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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[Histonet] histotech positions in San Diego area
Life is uncertain, but We may have an opening in the future. You may send resumes directly to this email address and I will forward them on to the appropriate people. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, April 26, 2010 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 77, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: [IHCRG] ER clone 1D5 or SP1 ? (Richard Cartun) 2. Region 1 conference (Amos Brooks) 3. Re: [IHCRG] ER clone 1D5 or SP1 ? (ancillaryp...@mac.com) 4. Mysterious artifact on GI biopsies HE stain (Urim, Lyudmila) 5. paraformaldayhde (Perry, Margaret) 6. RE: Mysterious artifact on GI biopsies HE stain (Weems, Joyce) 7. anti 8 hydroxyguanosine (Fabrice gankam) 8. RE: Mysterious artifact on GI biopsies HE stain (Mike Pence) 9. Any Histology openings in San Diego area? (Jill Cox) 10. (no subject) (Urim, Lyudmila) 11. Re: Two antibodies from the same host with tyramide (Johnson, Teri) 12. RE: (no subject) (Kaye Ryan) 13. RE: (no subject) (Nails, Felton) 14. RE: Best books for the HTL? (Morken, Tim) 15. RE: (no subject) (Jesus Ellin) 16. RE: [IHCRG] ER clone 1D5 or SP1 ? (Van Eyck, Deb) 17. RE: [IHCRG] ER clone 1D5 or SP1 ? (Patsy Ruegg) -- Message: 1 Date: Sun, 25 Apr 2010 15:04:28 -0400 From: Richard Cartun rcar...@harthosp.org Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? To: 'ih...@googlegroups.com' ih...@googlegroups.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu,jtay...@meriter.com Message-ID: 4bd459fb.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Taylor, Jean jtay...@meriter.com 4/23/2010 11:17 AM I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en -- Message: 2 Date: Sun, 25 Apr 2010 19:14:49 -0400 From: Amos Brooks amosbro...@gmail.com Subject: [Histonet] Region 1 conference To: histonet@lists.utsouthwestern.edu Message-ID: y2z582736991004251614m979ddea5yea99650b77ced...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi, I would like to ask if anyone has any photos of the Region 1 conference to consider emailing them to me. I was going to post some on the Region 1 Conference web page and possibly put some in the Paraffin Press. If you are interested, please drop me a line. Thanks, Amos amosbro...@gmail.com -- Message: 3 Date: Sun, 25 Apr 2010 20:36:18 -0400 From: ancillaryp...@mac.com Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? To: ihcrg Group (E-mail) ih...@googlegroups.com,histonet netserver histonet@lists.utsouthwestern.edu Message-ID: 0d48ea46-525d-45a6-bfcd-24662d59f...@mac.com Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon
[Histonet] Fibrinogen Ab
Does anyone know a fibrinogen antibody that reacts in pig? Thank you in advance. Joel R. Israel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Two antibodies from the same host with tyramide
Adam, I've been doing some searching and the tyramide shouldn't shelter subsequent IHC reactions, so I don't think that's it. I found evidence of co-localization of mRNA ISH signal with tyramide enhancement and IHC staining without amplification in a publication from J Histochem Cytochem. I'm actually quite surprised you are getting single staining using the goat anti-mouse antibody from RD systems. The one I found online (AF1002) has only been tested in Elisa and western blot, and I'm not seeing any sort of antigen retrieval in your protocol. I would suspect that most endothelial markers (with the exception perhaps of Factor VIII) would require it. The fact this is actually working for you in formalin fixed paraffin embedded mouse bone should be cause for celebration. I still would be interested in knowing if reversing the protocol would have any affect on the staining, especially if you can do the Cadherin first, verify staining briefly, then continue on with the other antibody and its detection and see what happens. Best wishes, Teri Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana EMA antibody
Histofriends, Has anyone had success staining an ependymoma with Ventanas EMA antibody? My meningiomas stain nicely. I have a case that I've stained with Dakos EMA on an Autostainer, and it shows the expected spotty pattern. Anyone out there have a successful protocol? Happy Monday, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: paraformaldehyde
Margaret, good luck getting 37 grams of powdered PFA to go into solution. Even making a 10% solution is difficult, usually requiring heat (60 degrees C) and/or sodium hydroxide to get it into solution. Once you do, it's going to want to repolymerize and you'll end up with a real mess. You'd do much better to use stock 37% formaldehyde you can buy commercially. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: paraformaldehyde
I ditto Teri's suggestion. In a recent email to you, I mentioned the dangers of using powdered paraformaldehyde. I have since stopped using it. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Monday, April 26, 2010 4:24 PM To: 'margaret.pe...@sdstate.edu' Cc: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: paraformaldehyde Margaret, good luck getting 37 grams of powdered PFA to go into solution. Even making a 10% solution is difficult, usually requiring heat (60 degrees C) and/or sodium hydroxide to get it into solution. Once you do, it's going to want to repolymerize and you'll end up with a real mess. You'd do much better to use stock 37% formaldehyde you can buy commercially. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gina Rodriguez is out of the office.
I will be out of the office starting 04/26/2010 and will not return until 05/03/2010. I will respond to your message when I return. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?
Thanks Michael I saw table 10 -but on page 16 they specifically only list clone 1294 and 312 (which is whose?) -they really don't clearly say all clones for PR listed in table 10. They also only refer to these two again on the shortened ASCO color plate handout Additional information in re: clinical question 2.1. Deb From: Michael Ho [mailto:michael...@sickkids.ca] Sent: Monday, April 26, 2010 4:13 PM To: Van Eyck, Deb; ancillaryp...@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? Hi Deb If you look at table 10 of the new guidelines you will see the ER/PR antibody clone list Michael From: ih...@googlegroups.com [ih...@googlegroups.com] On Behalf Of Van Eyck, Deb [deb.vane...@phci.org] Sent: April 26, 2010 12:29 PM To: ancillaryp...@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out --Hadi or Rich I know they only list two PR clones one is Dako 1294-what is the other 312? Deb From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of ancillaryp...@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Taylor, Jean jtay...@meriter.com 4/23/2010 11:17 AM I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ER clone 1D5 or SP1 ?
Hi A large number of studies have been performed which show that 6F11 will stain a number of tumours that 1D5 does not and I know a number of labs who have moved away from 1D5 because of this. Google 1D5 and 6F11 and you will find the articles. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Marrow Clots - falling off slides
Hi Histonetters! We have started to have an issue with our bone marrow clots falling off the slides. We are using plus slides, making sure they drain well (just like our other slides), but when we stain them routinely, we are getting a fair amount of tissue coming off the slides. It has been suggested that it's related to our using recycled xylene. Does anyone have any experience with recycled xylene and this type of tissue fall-off? Another suggestion was that the tissues sat in xylene too long. I don't see how that could happen, under routine conditions, but is that a possibility for causing this? All thoughts will be appreciated! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone Marrow Clots - falling off slides
recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols From: histot...@imagesbyhopper.com To: histonet@lists.utsouthwestern.edu Date: Mon, 26 Apr 2010 23:26:43 -0400 Subject: [Histonet] Bone Marrow Clots - falling off slides Hi Histonetters! We have started to have an issue with our bone marrow clots falling off the slides. We are using plus slides, making sure they drain well (just like our other slides), but when we stain them routinely, we are getting a fair amount of tissue coming off the slides. It has been suggested that it's related to our using recycled xylene. Does anyone have any experience with recycled xylene and this type of tissue fall-off? Another suggestion was that the tissues sat in xylene too long. I don't see how that could happen, under routine conditions, but is that a possibility for causing this? All thoughts will be appreciated! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
