[Histonet] Cryostat vs decal sections of bone
Hi all, last year sometime I was asked to budget for some equipment for our unit. Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IgG4 source
I have used invitrogen - mouse anti-human IgG4 Clone HP6025 (successfully) AR in 1mm EDTA Dilution 1:400 Control - tonsil (or Sjogren's Syndrome) Joao Pessoa jphistol...@gmail.com 2010/05/07 11:50 PM I need to find a source for an IgG4 antibody for use with IHC studies on human FFPE tissue. Any ideas? My preference would be for a concentrated IVD. Thanks, Joao Histo Tech ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 4500. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. _ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Number of Techs
Hello, I am looking to compile information on the average number of techs that different hospitals have in their histology labs. I would appreciate any feedback that I could get as far as how many techs are used to run hospital histology labs with a block count range of 200 to 400 blocks per day. Thanks for any input you can provide, Jason CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information (PHI) that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat vs decal sections of bone
Most definitely there are differences. Just consider that the bone will not be subjected to any chemicals because neither fixation nor demineralization will be required., René J. --- On Mon, 5/10/10, louise renton louise.ren...@gmail.com wrote: From: louise renton louise.ren...@gmail.com Subject: [Histonet] Cryostat vs decal sections of bone To: Histonet@lists.utsouthwestern.edu Date: Monday, May 10, 2010, 4:19 AM Hi all, last year sometime I was asked to budget for some equipment for our unit. Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Bone Frozen sections versus decalcified bone in paraffin
Louise, You wrote: last year sometime I was asked to budget for some equipment for our unit.Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. Yes there are distinct advantages for using Cryojane. Undecalcifed bone frozen sections are done in our lab in order to do immunostaining, either enzyme immunohistochemistry or immunofluorescence work on fresh undecalcifed bone frozen sections, especially when murine CD markers and GFP are compromised by aldehyde fixatives (PFA or NBF), acid decalcification, or aldehyde induced autofluorescence when working with GFP . There are ways to get around the autofluorescence problem. We use Cryojane for all undecalcifed bone frozen sections, but also section other very difficult frozen tissues. There are times using FFPE/decalcified bone is not going to stain successfully for either immuno or ISH methods IF the antigens are compromised by either mentioned methods, plus heat from paraffin processing. Cryojane is our backup method when things go awry, and our method of choice with most GFP work with murine nasal turbinates.Trying to do free hand frozen sections on undecalcified bone was an unsuccessful nightmare, very time consuming with terrible results (crunched up bone sections).Our problem has been too many murine CD markers we work with only stain on fresh frozen sections after solvent fixation. If most of your antigens (or ISH) stain successfully with decalcified, FFPE sections, then you may not need Cryojane but that is something you have to work out. We find Cryojane a necessity for our work. One can decalcify bone prior to fixation Mori et al then cut frozen sections, fix and stain - a long drawn out procedure for IHC. There are others who have success on undecalcifed bone frozen sections ( Kusser et al with excellent references) Both of these publications are now freely accessible in J Histochem Cytochem. ISH is more difficult on acid decalcified bone, where EDTA may have to be used for FFPE. There are some excellent publications on using decalcified bone (marrow) with ISH, the most recent from Gudrun Lang in Journal of Histotechnology, March 2010 describing kappa and lambda ISH on acid bone marrow biopsies. She listed several excellent references on ISH/IHC for FFPE bone samples. Gudrun participates on Histonet and should be able to drop a pdf of this publication to you. I have also done PLP fixed, EDTA decalcified bone, sucrose cryoprotected bone frozen sections mounted on Plus Charge slides, but prefixed bone frozen sections often release from the slide surface during staining. That is the joy of Cryojane, no release. Working with the instrument takes some practice, and there is an excellent publication by John Tarpley in J of Histotechnology for doing this. Cryojane is unique but be sure it fits in your cryostat before purchase. There is the Kawamoto tape method for bone frozen sections, but it requires purchasing a knife holder, and I believe, special knives. The section is NOT transferred to a slide, but remains on the tape where all staining takes place, then mount the tape onto a slide in a special way. Those who use it acquire beautiful staining results for both IHC and ISH. This method has been around for awhile and you have to purchase all the accessories and tapes from a company in Japan. I know that Jamie Erickson is using this and has also worked with Cryojane. Hopefully Jamie is looking in and can comment. Good luck, Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protocol Needed for Processing Previously Snap Frozen Tissue
Hello Histo-Netters: Does anyone have a protocol for post processing (FFPE) frozen tissue samples? Tanisha Neely HT(ASCP) mailto:tanisha.ne...@covance.com - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] Loss of tissue sections after antigenretrieval
Patsy is correct, there is a very good chance the retrieval is the issue. Another possible issue is the drying time. We find often times with tissue loss we can remedy that with extended drying times. If we get precut slides from an outside source, we dry them for 2 hours in a 58 degree oven to be cautious. Kelsey Jones Technical Consultant 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8969 (916) 746-8989 (fax) www.cellmarque.com **Check out Cell Marque on Facebook! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, May 08, 2010 4:11 PM To: 'Joseph N. Pastore'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] Loss of tissue sections after antigenretrieval Joe, Did you buy an array that was not cut with the tape transfer system and put onto polymer coated slides? If they had done that your tissues would not fall off, not sure of what to do after the fact, but you could certainly try a more gentle antigen retrieval technique such as waterbath (not sure of what you are using as your heat source?) sounds like your retrieval method is too harsh. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph N. Pastore Sent: Saturday, May 08, 2010 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigen retrieval Hi All, We have a problem. We are using a commercial tissue array and we find that the two most important tissues are lost during antigen retrieval. The tissues are samples of glioblastomas and are critical to our study. The antigen retrieval system we use is a proprietary 2 step system using a hi and lo pH. Both Ca chelators. Does anyone have suggestions about how we can prevent tissue loss ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Cryostat vs decal sections of bone
Hi Louise, Remember me? It's been a while. You asked if there were distinct advantages to using cryosections of bone over decalcified wax samples for IHC and ISH and the answer is yes, of course. Some antibodies and probes just won't work well in paraffin processed samples, either due to the decalcification process or the alcohol/solvent exposure. The ISH purists know that just paraffin processing can diminish signal greatly. Having said that, obtaining excellent sections of undecalcified, fresh frozen bone is tricky. Very tricky indeed, and what works one day doesn't work the next. You'll need to have at least one tungsten carbide knife for it, so make sure you also get the regular blade holder. I don't know if you still use steel knives on the cryostat, and if so you'll already have the correct blade holder, but we use disposables so we needed to order a separate one. We've had pretty good luck getting beautiful sections of fixed and decalcified cryosections and the cryojane, and this would work well for samples that just flat don't like paraffin processing but the antibody staining does ok with formalin and formic acid treatment. Having the system opens up this possibility for your studies as well. Best wishes always, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] per deim work
Hello Histoland I have question pertaining to temporary histology tech work/per Diem positions.I find myself currently out of work and am looking for my next adventure in the great world of histology and one of the ideas i had was to check out the possibility's of doing temp work.The only thing is I'm not sure if there is a temp agency for histo techs,or is there someone in histo land that currently working as a temp . I would greatly appreciate any help in my endeavors into this new world. Any information as to how much the pay rate or what to charge for daily work travel lodging or any other expenses that would be incurred in this type of work. Thanks for any help Richard Shook HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone marrow smear staining
Need Histonet help. Hematology recently purchased a new blood smear stainer that is producing variable staining. It is especially bad with the bone marrow smears, even when it is run through twice as was the norm with the old stainer (now gone). Until this is resolved, does anyone have a GOOD MANUAL wright-giemsa staining protocol you would like to share? Our pathologists are quite frustrated. They do not care for the May-Grunwald Giemsa that we do manually for our hematology oncologists. thank you for your quick responses!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Curling floating mouse brains
Hello everyone. I am slicing mouse brains that were perfused with 4% paraformaldehdye in PBS, then sunk in 30% sucrose in PBS. I want to cut the brains in 40 um sections, collect in wells of PBS, and do IHC. BUT my sections keep curling into little cannolis (or even tighter, like those pirouette cookies). Just a few weeks ago I sectioned 24 rat brains in this manner without any problem at all. I did the perfusions for all of these animals, so it is not an issue of inadequate perfusion. I asked someone if it could be related to size of tissue (mouse vs. rat), and it was suggested that 30% scucrose was too high for mouse brains, so I melted them out of frozen OCT (kept in a frig overnight in PBS), resunk brains in 20% sucrose/PBS and refroze in OCT again. They did indeed slice better at this conc of sucrose (no streaks, shredding, etc. when slicing) but the brains sure did curl still in the PBS!!! I thought the sections were too thick, so dropped to 30 um, and still there is curling. Even 20 um sections curl up in the PBS. It is not a humidity problem, or a blade problem, for I have tried to cut on another machine, and tissue still curled. Cutting temp is 13-14 degrees, same as used for rat brains. Tried different PBS--- same curling. I am at a loss. Any advice would be very helpful. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Delivery
Tissue specimens are brought directly to pathology. Cytology fluids are dropped off in Processing, because often fluids need to be shared between several departments, and our Cytology dept doesn't necessarily know that it is a shared specimen. Processing can look it up in the LIS. Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org Sent: Monday, May 10, 2010 11:35 AM To: histonet@lists.utsouthwestern.edu Cc: tbai...@mcleodhealth.org Subject: [Histonet] Specimen Delivery For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more spread out and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone marrow wright stain
Hello all, Need Histonet help. Hematology recently purchased a new blood smear stainer that is producing variable staining. It is especially bad with the bone marrow smears, even when it is run through twice as was the norm with the old stainer (now gone). Until this is resolved, does anyone have a GOOD MANUAL wright-giemsa staining protocol you would like to share? Our pathologists are quite frustrated. They do not care for the May-Grunwald Giemsa that we do manually for our hematology oncologists. thank you for your quick responses!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 78, Issue 12
We use this clone also. It is distributed by Invitrogen, but the company that makes it is CalTag. (Maybe Invitrogen owns them.) We use AR of ph 9.0 Tris (0.5mM)/EDTA (0.1mM) for 20 min, dilution of 1:2000, and detection of Dako Flex+. It is not an IVD, by the way; it¹s still RUO. Stephanie Rodriguez, HTL(ASCP), QIHC Senior Molecular Tech/IHC Tech III Phenopath Laboratories Seattle, WA On 5/10/10 10:43 AM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Message: 2 Date: Mon, 10 May 2010 13:26:20 +0200 From: Heather McCleod heather.mcl...@uct.ac.za Subject: Re: [Histonet] IgG4 source To: Joao Pessoa jphistol...@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: 4be8097c02cb00035...@gwiasmtp.uct.ac.za Content-Type: text/plain; charset=US-ASCII I have used invitrogen - mouse anti-human IgG4 Clone HP6025 (successfully) AR in 1mm EDTA Dilution 1:400 Control - tonsil (or Sjogren's Syndrome) Joao Pessoa jphistol...@gmail.com 2010/05/07 11:50 PM I need to find a source for an IgG4 antibody for use with IHC studies on human FFPE tissue. Any ideas? My preference would be for a concentrated IVD. Thanks, Joao Histo Tech ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Region II Managers Forum
Region II Managers on the Histonet: Opportunity to attend the Managers Forum - Region II Meeting/Atlantic City West, NJ. Date: June 10 Time: 1:30-5:00PM Session Title: Manager's Forum: Roundtable Discussion for Anatomic Pathology Managers and Core Labs Panel of Experts: Carol Barone Director Histotech Core Lab, Nemours - A.I. duPont Hospital for Children, Wilmington, DE Janice Alvarez Anatomic Pathology Project Manager, Johns Hopkins Hospital, Baltimore, MD Melissa Gannon Histology Manager, DIANON/Laboratory Corporation of America, Raleigh, NC Moderator: Diana Goodwin, Previous Anatomic Pathology Supervisor Pennsylvania Hospital in Phila,PA This informal discussion among peers, will be conducted by and for individuals who are in a supervisory or managerial roles encompassing technical and/or administrative oversight in histotechnology. Registrants are encouraged to submit topics of interest, or concern via e-mail to :dgoodwin...@comcast.net at least two weeks prior to the event. Participants will earn 3 CEU for attending this half day workshop. Cost of this session is $40 for state society or Region II members or $60 for non-members. Deadline is May 20th To download a meeting brochure and registration information, go to the Region II meeting announcement under the state meeting information on the NSH website at www.nsh.org/content/region-ii-meeting. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Delivery
Here, all the surgical specimens are delivered directly to the Histology lab. All other specimens are delivered to the Specimen processing area (SPA). This way all specimens that need to be shared can be done there. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org [mwh...@mcleodhealth.org] Sent: Monday, May 10, 2010 2:35 PM To: histonet@lists.utsouthwestern.edu Cc: tbai...@mcleodhealth.org Subject: [Histonet] Specimen Delivery For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more spread out and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Labs work schedules
I have an important question to pose to all of you Histonetters. We currently have 5 Histotechs who work from 4:00 am to 4:30 pm Monday thru Friday. One comes in at 4 am to embed, another comes in at 5 am to help finish embedding and start cutting blocks. The others come in from around 7 am to 8 am. We average around 200-250 blocks per day with an average of 350-400 slides cut per day. IHC and Special staining is averaging around 145-200 slides per day with that number increasing. So the burden of the work rests on these 5 techs in the morning. We have a tech from Cytology who performs all of the IHC staining using two Ventana Benchmark stainers. The problem is our IHC volume keeps increasing so that two runs a day are not keeping up with the volume. We are playing around with the idea of adding an additional shift so that we have personnel here later in the day to run the IHC and special stainers and to embed and cut small biopsies and breast specimens. Below is a list of questions that I would like for you to answer to assist us in making these crucial decisions. 1. What are your staff schedules? HT's, PA's and Lab assistants. 2. On average, how many blocks and slides do you cut per day? 3. If you have more than one shift, what hours do they work and what job functions do they perform? 4. Does any of your staff work on the weekend and if they do what is their job function? 5. What kind of QC/QA program do you utilize in your laboratory? Thank you so much for you help. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P/T Certified Techs Needed (Multiple Openings)
I have job openings in Santa Rosa, Folsom, and Torrance, California for part-time certified histotechs. Great opportunity to work in brand new, well furnished, high quality, in-office path labs with great physicians. Competitive pay and flexible hours. Send resumes to Timothy Garcia-Jay at tja...@yahoo.com Thank you. Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Are Histotechs considered exempt employees?
Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
