Re: [Histonet] need tips for cross-sectioning of cortical bone
Why not embed in resin (MMA) and take thicker sections and then grind/ polish them down? If you went this route, you could then use flourescent labels and quantify mineral apposition rate and bone formation rate. Let me know if you are interested. I can help you get started and direct you to low cost equipment options. Jack On Apr 22, 2010, at 9:58 AM, Connolly, Brett M brett_conno...@merck.com wrote: A colleague is having trouble getting wrinkle-free sections of decalcified, paraffin embedded femur. Any tips?? Thanks, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html ) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Are Histotechs considered exempt employees?
-Original Message- From: Thomas Jasper Sent: Tuesday, May 11, 2010 3:45 AM To: 'Anthony Sandoval' Cc: 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Are Histotechs considered exempt employees? Anthony, Don't know where you live in Cali or where you work. But if you are an HTL and have any decent skill/experience, I would think you are being totally ripped off. The cost of living in most parts of Cali alone makes me wonder about this salary. And when you ask about being exempt...I'm assuming you are exempt? If this is the case again a total rip off. I think you need to look for work elsewhere and check into wages. New students that are registry eligible start out much higher than that to my knowledge. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor CORPS Bend, Oregon -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered exempt employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Idea for a new recycler
If formalin, xylene, hemo-de, Formula 83 and alcohol were all collected as one waste stream, no recycler could separate this waste into recycled formalin, recycled solvent (xylene/hemo-de/F83), and recycled alcohol all in one run due in part to the small range of boiling points and in part to the azeotropes formed between the various chemicals. In this particular scenario, the alcohol and the formalin would distill over together along with any water in the formalin, and there would be a considerable amount solvent in the alcohol making it contaminated and not of any use in the lab. If you have any questions, please contact us off site and we'll be happy to assist you. Best Regards, Elizabeth Sell CBG Biotech 1-800-941-9484 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. clo test (Tench, Bill) 2. IDEA FOR A NEW RECYCLER (Madary, Joseph) 3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz) 4. CD 133 (Ingles Claire ) 5. Disposable blade holder - amateur microscopist lookingfor a cheap one! (Gordon Brown) 6. Disposable blade holder - amateur microscopist lookingfor a cheap one! (Gordon Brown) 7. NSH Region II Meeting-discount hotel rate deadline is coming up! (Goodwin, Diana) 8. dehydration of hydrated slide (Eva Permaul) 9. RE: dehydration of hydrated slide (Sebree Linda A) 10. RE: dehydration of hydrated slide (Mauger, Joanne) 11. B5 fixative (histot...@imagesbyhopper.com) 12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED)) 13. RE: Responses to IHC CAP Validation question (tonia.richm...@gracepathology.com) 14. Starting up new lab (Shaw, Sharon) 15. Formalin fixation time for breast specimens (Richard Cartun) 16. RE: Responses to IHC CAP Validation question (bsulli...@shorememorial.org) 17. Re: myocyte damage (Merced M Leiker) -- Message: 1 Date: Wed, 5 May 2010 13:57:20 -0700 From: Tench, Bill bill.te...@pph.org Subject: [Histonet] clo test To: histonet@lists.utsouthwestern.edu Message-ID: 2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local Content-Type: text/plain; charset=us-ascii The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *** mail2.pph.org made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - -- Message: 2 Date: Wed, 5 May 2010 17:22:22 -0400 From: Madary, Joseph mada...@medimmune.com Subject: [Histonet] IDEA FOR A
RE: [Histonet] Are Histotechs considered exempt employees?
$16.15 per hour. They are getting a great deal. I believe you should be making at least double that. What I believe is happening is that a lot of offices are now starting to open their own labs and they are trying to get us cheap. We all need to stick to our guns and not let them get us cheaper than we are worth. Yes, I realize that San Francisco is more but the starting wage for a HT's should be $25.00 to $30.00 an hour to start for a newly certified tech. No matter of location in California. You can always negotiate higher if you have more experience and more certifications. Just my two cents worth, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered exempt employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 78, Issue 12
Hi Jason We cut 200+ a day - we have histo tech in at 400, 430, 500 and 2 at 6am. One is student in training, and one is on the brink of retirement, so there will be only 1 coming in at 6. I am the coordinator the dept. so after cutting is finished, and if we are at full staff, I then switch to desk work. Nancy Schmitt HT, MLT (ASCP) Histology Coordinator Dubuque, IA Message: 3 Date: Mon, 10 May 2010 08:43:30 -0400 From: Jason Keller jke...@capefearvalley.com Subject: [Histonet] Number of Techs To: histonet@lists.utsouthwestern.edu Message-ID: 7d41e97c787df44996e0055c81703663f17...@ntexchange3.capefear.local Content-Type: text/plain; charset=us-ascii Hello, I am looking to compile information on the average number of techs that different hospitals have in their histology labs. I would appreciate any feedback that I could get as far as how many techs are used to run hospital histology labs with a block count range of 200 to 400 blocks per day. Thanks for any input you can provide, Jason NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytotech Position
Hi everyone! If anyone knows a cytotechnologist interested in a position, we have one here in Lakeland, Florida. It is a full-time position, M-F and candidate must have FL state license or be eligible to obtain one. Please contact me for further information. Thank you in advance. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Delivery
We have a central drop off area. Occasionally we will have shared specimens with microbiology and shared fluid specimens with the clinical lab. This helps ensure all tests ordered are performed. We pick up our specimens from the central area. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org Sent: Monday, May 10, 2010 1:35 PM To: histonet@lists.utsouthwestern.edu Cc: tbai...@mcleodhealth.org Subject: [Histonet] Specimen Delivery For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more spread out and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Are Histotechs considered exempt employees?
Starting out at $16 an hour is not acceptable. I've hired recently graduated students at a better pay than that. And this is Florida where the cost of living is a lot cheaper than Cali. Hope this helps. :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Heckford, Karen - SMMC-SF karen.heckf...@chw.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 05/11/2010 07:15 AM To Anthony Sandoval sandoval.1...@hotmail.com cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Are Histotechs considered exempt employees? $16.15 per hour. They are getting a great deal. I believe you should be making at least double that. What I believe is happening is that a lot of offices are now starting to open their own labs and they are trying to get us cheap. We all need to stick to our guns and not let them get us cheaper than we are worth. Yes, I realize that San Francisco is more but the starting wage for a HT's should be $25.00 to $30.00 an hour to start for a newly certified tech. No matter of location in California. You can always negotiate higher if you have more experience and more certifications. Just my two cents worth, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered exempt employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] per deim work
Have you tried Aerotech scientific(sp). I know they are in several states you should look them up in your area. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of richard shook Sent: Monday, May 10, 2010 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] per deim work Hello Histoland I have question pertaining to temporary histology tech work/per Diem positions.I find myself currently out of work and am looking for my next adventure in the great world of histology and one of the ideas i had was to check out the possibility's of doing temp work.The only thing is I'm not sure if there is a temp agency for histo techs,or is there someone in histo land that currently working as a temp . I would greatly appreciate any help in my endeavors into this new world. Any information as to how much the pay rate or what to charge for daily work travel lodging or any other expenses that would be incurred in this type of work. Thanks for any help Richard Shook HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Are Histotechs considered exempt employees?
Anthony, You live in CA, work 50 hour weeks and you are a HTL with an unprecedented and valuable resource at your disposal, Histonet and you know how to use it. For this you get paid $16.15/hour by what appears to be an employer who isn't grateful to have you. I'd start looking for another job. Just my humble opinion. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello P Please consider the environment before printing this email. On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote: Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Are Histotechs considered exempt employees?
I am so with you on this one. HT's need to start demanding and higher wages everywhere. We are a rare breed with special talents. Without a good tech you do not get good slides!! We are not a dime a dozen. AUGH! I know just a hour north of where I am the wages can be $10-$15.00 less. Not much difference in living expenses either. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, May 11, 2010 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Are Histotechs considered exempt employees? Anthony, You live in CA, work 50 hour weeks and you are a HTL with an unprecedented and valuable resource at your disposal, Histonet and you know how to use it. For this you get paid $16.15/hour by what appears to be an employer who isn't grateful to have you. I'd start looking for another job. Just my humble opinion. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello P Please consider the environment before printing this email. On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote: Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] need tips for cross-sectioning of cortical bone
You did not say whether these are cross sections or mid sagittal? Cross sections are always tougher. Key is to make sure the cortical bone is well processed and infiltrated with a hard paraffin e.g. Tissue Prep 2 (Fisher Scientific ala Thermo Scientific). Try this old bonehead trick is cut tiny V-shaped notches with razor blade or used microtome blade on the sides of block, parallel to the blade. You may have to do this top and bottom too. Take care to NOT make these notches huge. This permits the paraffin of each section in ribbon to expand, relax onto the water bath along with the bone section. Another trick is try laying section on RT 5 to 10% alcohol, pick up on slide, then go to warm water bath, lower section slowly to flattening. The key here is to NOT let upper part of paraffin of a section totally release from slide while going into warm water (at an angle) in other words, the section flattens while paraffin portion is still attached to slide during flattening. Also, change the blade frequently. Sharpest possible edge helps, and hopefully high profile which is more stable than low profile for decalcified bone microtomy. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, May 11, 2010 5:03 AM To: Connolly, Brett M Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone Why not embed in resin (MMA) and take thicker sections and then grind/ polish them down? If you went this route, you could then use flourescent labels and quantify mineral apposition rate and bone formation rate. Let me know if you are interested. I can help you get started and direct you to low cost equipment options. Jack On Apr 22, 2010, at 9:58 AM, Connolly, Brett M brett_conno...@merck.com wrote: A colleague is having trouble getting wrinkle-free sections of decalcified, paraffin embedded femur. Any tips?? Thanks, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html ) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Information from ESET Smart Security, version of virus signature database 5103 (20100510) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5103 (20100510) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5105 (20100511) __ The message was checked by ESET Smart Security. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Article for Er/PR vaidation
Hello everyone I have been trying to locate this article on line and I'm either challenged or its not there. Its from the Arch Pathol Lab Med (in press) it says in press so I don't know what that that means but its not available off the Journals web site Fitzgibbons, P. L. , D. A. Murphy , M. E. H. Hammond , et al. Recommendations for validating estrogen and progesterone receptor immunohistochemistry assays. Arch Pathol Lab Med (in press). Does anyone have a copy of this that they are willing to forward to me, the new ER/PR guidelines that just came out reference this article for validation, even through at the end of the article there is a statement --- there is no universal acceptable procedure for validating tests any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processing/cutting cell blocks
We typically use a media called histogel for our cytology cell blocks with very good results. Our pathologists also send us clots from other locations that they are collecting like a bone marrow aspirate clot. The FNAs are expressed into a lid and allowed to clot. We are processing these on an overnight run used for all our other cases. The histotechs are having a great deal of trouble cutting these as they are very dried out and break apart on the water bath. Is there any techniques you can recommend to get better results when cutting specimens collected this way? I would think we need to process them on a shorter program to begin with. Thank you in advance for any suggestions. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3
Thanks to all for your suggestions for cutting cross sections of cortical bone. I have passed them along. Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray Koelling's suggestion in the archives using an Ab from BD Biosciences. Is it cat # 550295? What others if any do you suggest? HEIR ? protocol tips? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Forwarding Request for Control Mehtod
I wonder if you could help me find someone who would kindly donate a paraffin tissue block containing candida or other fungi to be used as a control block. We have had difficulty in obtaining such tissue and have tried all the hospitals in northern Ireland for a tissue block suitable enough to act as a control. Many of the pathology departments are in the same position as ourselves in that the control tissue being used is getting in short supply. I tried to make one using a culture from microbiology with fresh lambs lung from a local abattoir but unfortunately the tissue seems to stain with PAS as well and the candida where obscured although visible probably due to post mortem changes in the tissue. Alternatively have any of your members successfully produced fungal control material by other means. Thanking you in advance Ian Clarke Biomedical Scientist Cellular Pathology Department Craigavon Area Hospital Sothern Health and Social Services Trust. 66 Lurgan Road Craigavon BT63 5QQ Northern Ireland William DeSalvo, B.S., HTL(ASCP) _ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccountocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3
Dako has a rabbit polyclonal that works in rat. The other thing with the bone, is place the slide with a section on a flat hotplate (60 C) until it turns clear - not too long or the articular cartilage will flip. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, May 11, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3 Thanks to all for your suggestions for cutting cross sections of cortical bone. I have passed them along. Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray Koelling's suggestion in the archives using an Ab from BD Biosciences. Is it cat # 550295? What others if any do you suggest? HEIR ? protocol tips? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CYP.07680 - cytology cross contamination
Hello all, For CAP policy CYP.07680 for procedures to prevent cross-contamination of specimens during processing and staining - what are your labs doing? I work in a small lab so the histotechs process the cytology specimens and the pathologists read the slides (we have no PA's or cytoprep techs or cytotechs to screen slides). We also process only non-GYN, so we don't have to worry about GYN/non-GYN cross contamination. The notes under this policy say procedures must prevent cross-contamination between highly cellular specimens and suggest the screening method of toluidine blue stain to determing if specimens are highly cellular. Does anyone use the toluidine blue for this purpose? If so could you tell me the procedure for toluidine blue you use? And how do you determing which specimens you stain with toluidine blue and what qualifies as highly cellular. If so do you retain these toluidine blue slides for any period of time? CAP policy also suggests inserting a clean blank slide in each stain run and examine for contamination. Is anyone doing this? We have been inspected before with no problems with this CAP question, but I just want to make sure we are doing everything we can to prevent cross-contamination. Thanks in advance for your input! Brandi Higgins, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] L-DNAse II vs neutrophil (leukocyte) elastase inhibitor
Hi guys Would like to know if any of you have used the L DNAse II or the serpin B1 antibody in the rat. very few references mentioning this antibody. Sigma has one prestige antibody for serpin B1 but do no know if it will work on rats. I was wondering if the DNAse II is the equivalent of the L DNAse II (product of the degradation of serpin) thanks for your help ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ICC
Hello all, I am starting ICC and flourescence for the first time. I have done tons of IHC in the past with DAB and AEC= staining, but I am slightly confused with the way the flourescence works. Do you have to buy a secondary that is already tagged, or is= there some system where it attaches to the secondary like DAB does? = Does anyone have any advice or some site where I can go to get some info on this. Also, what do you use for positive and negative controls fo r ICC? Thanks fellow histo hotties!! [DEL: Sarah= Goebel, B.A., HT (ASCP) :DEL] Histotechni= cian XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Genemed antibodies
Hi, Does anybody have experience with Genemed Biotechnologies antibodies and/or probes of Histosonda? Kind regards, A.Van der Aa Confidentiality Note: This information and the attached file(s) are considered trade secret, confidential and/or property by Sakura Finetek Europe B.V. Any use, disclosure of reproduction of these documents by anyone other than the addressee is prohibited. If this E-mail has been received by anyone other than the addressee, please call +31 (0)88 592 00 00 immediately to arrange for the document(s) to be returned. Sakura can never be legally bound by the acceptance of any supposed offer in an electronic message. Please reply or send your e-mail to: sak...@sakura.eu Sakura Finetek Europe B.V. P.O. Box 362, 2400 AJ Flemingweg 10A, 2408 AV Alphen aan den Rijn The Netherlands Tel. +31 (0)88 592 00 00 Fax +31 (0)88 592 00 01 KvK/Chamber of Commerce Leiden 28065449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CYP.07680 - cytology cross contamination
Hi Brandi, The clean, blank slide is some times inserted between cases in the staining rack to determine if floaters are present. It's sort of a retroactive test - if you see cells on the blank slide, then you already have cross-contamination and you need to re-prep and restain the cases separately. Some people feel that you've caught the floaters on your clean slide and the problem is solved, but there's no guarantee others won't be present on your specimen slides, too. Toluidine blue is an example of a stain you could use to determine cellularity; another option often used is a modified Wright Giemsa stain such as Diff-Quik. You're not looking to make a final diagnosis, but to make a quick preparation and use a simple stain just to see if there are a lot of cells present. This is a more proactive test. The idea is that highly cellular specimens have an increased likelihood of shedding cells into the stains and contaminating other cases; it also can be used to identify obviously malignant cases, which should definitely be stained separately. If you're using a rapid stain to look for high cellularity, you need to centrifuge and concentrate the specimen(s) first, just as you would in regular prep. The Diff-Quik stain is a Romanovsky stain and is used on air-dried slides, so there's no need for fixation. Hope that helps - Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.je...@gwu-hospital.com From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins [brandihigg...@gmail.com] Sent: Tuesday, May 11, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CYP.07680 - cytology cross contamination Hello all, For CAP policy CYP.07680 for procedures to prevent cross-contamination of specimens during processing and staining - what are your labs doing? I work in a small lab so the histotechs process the cytology specimens and the pathologists read the slides (we have no PA's or cytoprep techs or cytotechs to screen slides). We also process only non-GYN, so we don't have to worry about GYN/non-GYN cross contamination. The notes under this policy say procedures must prevent cross-contamination between highly cellular specimens and suggest the screening method of toluidine blue stain to determing if specimens are highly cellular. Does anyone use the toluidine blue for this purpose? If so could you tell me the procedure for toluidine blue you use? And how do you determing which specimens you stain with toluidine blue and what qualifies as highly cellular. If so do you retain these toluidine blue slides for any period of time? CAP policy also suggests inserting a clean blank slide in each stain run and examine for contamination. Is anyone doing this? We have been inspected before with no problems with this CAP question, but I just want to make sure we are doing everything we can to prevent cross-contamination. Thanks in advance for your input! Brandi Higgins, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Labomed LX400 compound microscope
Hello, Does anyone have any experience with that Labomed Lx 400 compound microscope? It is new and it is cheap (scope with Moticam 2000 camera - ~$2,000), but I cannot find any reviews on it. I'm looking for a simple microscope to take digital pictures of cross sections of rat spinal cords for some volumetric measurements. Steve ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Forwarding Request for Control Mehtod
Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod I wonder if you could help me find someone who would kindly donate a paraffin tissue block containing candida or other fungi to be used as a control block. We have had difficulty in obtaining such tissue and have tried all the hospitals in northern Ireland for a tissue block suitable enough to act as a control. Many of the pathology departments are in the same position as ourselves in that the control tissue being used is getting in short supply. I tried to make one using a culture from microbiology with fresh lambs lung from a local abattoir but unfortunately the tissue seems to stain with PAS as well and the candida where obscured although visible probably due to post mortem changes in the tissue. Alternatively have any of your members successfully produced fungal control material by other means. Thanking you in advance Ian Clarke Biomedical Scientist Cellular Pathology Department Craigavon Area Hospital Sothern Health and Social Services Trust. 66 Lurgan Road Craigavon BT63 5QQ Northern Ireland William DeSalvo, B.S., HTL(ASCP) _ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccountocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: L-DNAse II vs neutrophil (leukocyte) elastase inhibitor
Hi guys Would like to know if any of you have used the L DNAse II or the serpin B1 antibody in the rat. very few references mentioning this antibody. Sigma has one prestige antibody for serpin B1 but do no know if it will work on rats. I was wondering if the DNAse II is the equivalent of the L DNAse II (product of the degradation of serpin) thanks for your help ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
