RE: [Histonet] Refrigerate formalin?
I had a retic kit that was put in the refrigerator by one of my collegues. The formalin was stated to be stored at room temp, but was left in the box. The cold temperature broke the formalin down and it no longer worked to reduce the silver. My suggestion would be to keep them at room temperature to be on the safe side. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 28, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerate formalin? We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NEW Opportunity with a World Leader in IHC in New England!
Good Morning All, Our team at Personify is currently partnered with a World Leader in IHC that is currently looking for a Field Support Specialist in the New England area. This is the perfect opportunity for a histotech to move off the bench and get into the field on the manufacturers side! The position offers the following: Base Salary! Bonus! Car Allowance and Paid Expenses! Outstanding Benefits! Opportunity for advancement! Please contact me immediately to learn more! Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 http://www.personifysearch.com/ www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Refrigerate formalin?
I agree with Rene and Mark. Geoff Breeden, Sara wrote: We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FAST interpretation
Hi, I'm using FAST profile (Fast green, Alcian Blue, Safranin-O, and Tartrazine) to stain my human Intervertebral Discs. how can I figure out which color resembles which type of tissue? Regards -Sarah ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cool Formalin
Thanks to everyone that replied to my question about refrigerating 10% NBF for up to 6 weeks. I'll fold your information into the decision. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] plastics
Hi histoland, Thinking of getting into plastics and need to know information, pros/cons about them. I've never worked with anything else except paraffin. I will be embedding mostly osteosarcomas. Can someone please give me a call or email me. All suggestions are very appreciated. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dix...@ufl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p53 IHC staining in mouse
Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antibodies (ab32049 and ab4060) and both are not working correctly on mouse tissue. Does anyone know of a p53 antibody that is known to work in mouse tissue? Thanks, Ross ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] knife holder
Greetings histonetters!!! Does anyone out there have a paraffin knife holder (knife holder B) for a Leica SM2500 polycut microtome that you might like to part with. I am in need of finding one. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dix...@ufl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Using GEWF solution and IHC staining
GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution, Davidson's fixative (3 parts water, 3 parts alcohol, 2 parts 37% formaldehyde, 1 part glacial acetic acid), and various proprietary products (Dissect-Aid, O-Fix, and others) are all used to fix fatty tissue in order to recover as many lymph nodes as possible. The unfixed fatty tissue is separated from the rest of the specimen and fixed overnight in the disclosing fixative. A good many pathologists, including me, like these fixatives for colon cancers. I've also used it for radical neck dissection specimens. It's not necessary for axillary dissection specimens for breast cancer, since the lymph nodes are big and easy to find. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] : Gulf oil ? Histonet Digest, Vol 80, Issue 34
Yes, ORO stains lipids but I don't think it would work for crude. Would you look for artifact and tissue reaction? Pam Vlies, HT-ASCP Waukegan IL Sent on the Sprint® Now Network from my BlackBerry® -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Gordon and Sweets retic stain (Johnson, Nacaela) 2. RE: Histonet Digest, Vol 80, Issue 33 (Joanne Clark) 3. Sakura VIP6 update (Cynthia Pyse) 4. dissection aide (Tench, Bill) 5. Refrigerate formalin? (Breeden, Sara) 6. Re: Refrigerate formalin? (Rene J Buesa) 7. REFRIG FORMALIN (Tench, Bill) 8. Re: Refrigerate formalin? (Mark Ray) 9. Re: ? on Gulf oil spill (Lesley Weston) 10. PECAM-1 (sc-1506R) staining (Victor Wong) 11. RE: Refrigerate formalin? (Johnson, Nacaela) 12. NEW Opportunity with a World Leader in IHC in New England! (Matthew Ward) 13. Re: Refrigerate formalin? (Geoff McAuliffe) 14. FAST interpretation (sarah Tabatabaei) 15. Cool Formalin (Breeden, Sara) 16. plastics (Dixon,Maryann) 17. knife holder (Dixon,Maryann) 18. p53 IHC staining in mouse (Ross Benik) -- Message: 1 Date: Wed, 28 Jul 2010 12:11:42 -0500 From: Johnson, Nacaela nacaela.john...@usoncology.com Subject: [Histonet] Gordon and Sweets retic stain To: histonet@lists.utsouthwestern.edu Message-ID: 6dbd71c31d7e82e5d3dfbc202d260245d...@txhous1eb012.uson.usoncology.int Content-Type: text/plain; charset=us-ascii Hello! I am having a problem with my tissue washing off of the slides during the retic stain. The majority of the tissue is falling off during the Working silver solution step because of the alkalinity of the solution. The tissue is bone marrow and I use Halt in my water bath. My thoughts are to add a mild acid to the working solution to bring the pH down, but I am not sure of what the effects are on the solution. I do not want the silver to not impregnate. Has anyone had this problem before and found a solution? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre -- Message: 2 Date: Wed, 28 Jul 2010 12:34:49 -0600 From: Joanne Clark jcl...@pcnm.com Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 33 To: histonet@lists.utsouthwestern.edu Message-ID: 0cda5e1e01301f4880a8a7a8bcbda39c012f7...@mail.pcnm.com Content-Type: text/plain; charset=iso-8859-1 We use a similar product called Dissect Aid, from Decal Corp. IHC staining has never been an issue; we never run IHC on the lymph nodes from colon cancer cases. We usually do the IHC on the tumor itself which is fixed in formalin. This kind of product is very useful in helping to find all those pesky little nodes that like to hide in the fat by turning them white. Cancer protocols require that at least 13 nodes be submitted. As histo supervisor, I also do the grossing in my facility, so I understand why your PA is interested in this kind of product (it can be difficult to come up with this magic number of 13 without it). However, if you do run IHC on the nodes from these cases, you would indeed have to revalidate your markers with this as your primary fixative or as a post fixative. Joanne Clark, HT, MLT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM - Message: 2 Date: Tue, 27 Jul 2010 15:03:33 -0300 From: Hayes, Randi (HorizonNB) randi.ha...@horizonnb.ca Subject: [Histonet] Using GEWF solution and IHC staining To: histonet@lists.utsouthwestern.edu Message-ID: c2889f00e87e8d4ea5798737d8f7f362a73...@rhaex1.rha-rrs.ca Content-Type: text/plain; charset=iso-8859-1 At a recent conference, our PA learned of using GEWF (glacial acetic acid,
[Histonet] labeling of recycled chemicals
Dear Histonetters, If you use a recycler for xylene and/or alcohol, how are you labeling the recycled containers? Do you assign a lot # to each carboy? Do you keep a log or just label any container filled from a carboy with the lot # and the %, in the case of alcohols? Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: labeling of recycled chemicals
Sandy, We keep a log that says the alcohol was tested for %, date and tech. The recycled alcohol goes into already labeled containers that notes that it is recycled and the %. The recycled Formula 83 is tested for purity and entered into the log the same way the alcohol is. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Thursday, July 29, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Cc: Dachel, Susan K. Subject: [Histonet] labeling of recycled chemicals Dear Histonetters, If you use a recycler for xylene and/or alcohol, how are you labeling the recycled containers? Do you assign a lot # to each carboy? Do you keep a log or just label any container filled from a carboy with the lot # and the %, in the case of alcohols? Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: p53 IHC staining in mouse
I get my mouse p53 Ab from LabVision... http://www.labvision.com/ab.cfm?First=AntiBodySecond=9006 -- Message: 18 Date: Thu, 29 Jul 2010 10:56:56 -0600 From: Ross Benik r...@premierlab.com Subject: [Histonet] p53 IHC staining in mouse To: histonet@lists.utsouthwestern.edu Message-ID: ee33be5c905a3046a7ff8f58a64c8e4b0a8...@server.premierlab.local Content-Type: text/plain; charset=us-ascii Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antibodies (ab32049 and ab4060) and both are not working correctly on mouse tissue. Does anyone know of a p53 antibody that is known to work in mouse tissue? Thanks, Ross -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 34 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Technician position available
Northwest Pathology has an opportunity for an Immunohistochemistry Technician, ASCP certified or registry eligible preferred, to join our progressive laboratory. This full-time position requires two to three years of hands on immunohistochemistry experience with QIHC certification or eligibility for certification. Molecular experience is desirable. Responsibilities include a variety of routine and specialized laboratory procedures with an opportunity to learn new testing as we expand our services to the community. NW Pathology, a private laboratory located in Bellingham, WA, which diagnoses over 30,000 specimens a year is located between Seattle, WA and Vancouver, BC, and borders the North Puget Sound including the San Juan Islands. Wilderness areas with glacial rivers and the North Cascades National Park are nearby. We offer a generous comprehensive benefits package, including health and dental insurance, paid time off, 401(k) savings plan and profit sharing. For more information please contact: Jennifer Bull Histology Supervisor - NW Pathology 3614 Meridian St, Suite 101 Bellingham, WA 98225 360-734-2800 x503 jennifer.b...@northwestpathology.commailto:jennifer.b...@northwestpathology.com mailgate.hinet.org made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Film Removal
Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Film Removal
We soak the slides in acetone for 5-10 minutes and the film will peel off very easily. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Paula Lucas Sent: Thursday, July 29, 2010 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Film Removal Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Film Removal
The slips will not come off with xylene Soak in acetone for 5mins The slip swells and falls off Without letting the slide dry or turn milky white, dip into xylene a few times If milkiness appears, dip in clean acetone a few times and back to xylene Recoverslip as and when needed Simple really AnnieinArabia Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Paula Lucas plu...@biopath.org Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 29 Jul 2010 12:17:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Film Removal Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Film Removal
We use a 50-50 solution of xylene and acetone. Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off. If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 sscal...@beaumonthospitals.com Paula Lucas plu...@biopath.org 7/29/2010 3:17 PM Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Film Removal
We 1st use straight acetone and leave the slide in anywhere from 1 to 3 minutes, then 20 seconds in 50/50 acetone/xylene, then dip in straight xylene. Depending on how long the slide has been coverslipped, the times will need to be adjusted. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlas...@pathgroup.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Thursday, July 29, 2010 3:37 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Film Removal We use a 50-50 solution of xylene and acetone. Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off. If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 sscal...@beaumonthospitals.com Paula Lucas plu...@biopath.org 7/29/2010 3:17 PM Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Refrigerate formalin?
Hey, Sara! Congratulations. Enjoy all them Hassles and Advantages in your NEW lab. Best wishes, Carl NB: re Formalin fixation...one could argue that one should store such-fixed specimens in 90% alcohol, after the appropriate Formalin fixation time? OR...30% Sucrose: now, THAT is a PRESERVATIVE and NOT a Fixative ;-) Howeverloadsa variables. For eg: ER/PR Ab immunolocalisation ..I understand that , for Clinical Labs ,there is a window of acceptible Formalin fixation? So, if we store specimens in Formalin after diagnosisall them friggin defined/optimised conditions are OUT of the window? NB: why refrigerate Formalin? It will do the same thing but, slower. Stickthem tissues into 30% Sucrose? Well, nobody knows anything rigorous re actuality of Formalin fixation. Still...;-) Empirically, Carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thomas Crowell is out of the office.
I will be out of the office starting 07/29/2010 and will not return until 08/02/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL exam
Does anyone have any advice on good study aids, areas of prep to concentrate on, or any test taking strategies that helped them? I have been pouring over the 3rd ed of histotechnology, a self instructional text for months but since I have started to look at the ASCP/ NSH discussion boards im getting the feeling that it is just not enough. Thanks for any help. -Brian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslipping Problem - Please Help!
Dear Histonet members, I have been cryosectioning Xenopus laevis embryos this summer that have undergone a whole mount chromogenic in situ. The embryos are then fixed overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS until they are cryoprotected in a sucrose solution at least overnight at 4 C. Then they are put in TBS tissue freezing medium for 2-3 hours at room temperature before being frozen and sectioned. The sections come out looking very nice with good morphology, but when they are coverslipped (2 1 minute washes in 1 x PBS to dissolve the tissue freezing medium) with VectaMount AQ aqueous mounting medium, the sections turn very dark under the microscope, especially the gut region. They are so dark that it becomes difficult to see the signal from the in situ. Any help or advice would be GREATLY appreciated! Thank you for your time, Brian Rabe The College of William and Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HTL exam
Brian, What helped me a lot with stains, fixatives, etc, was to make a chart of each of the stain or fixative families (silver, trichromes, etc) and list the method steps of each, components used, and purpose of the components. That put in perspective the reasons for the differences, which are mysteries otherwise! I also used the NSH study guides, and any other book or study guide I could find to refer to. I was also lucky that I had a group of four people who were studying for the test and we spent a YEAR in a once-weekly study group going through each chapter in detail (Sheehan at that time). That was great for motivation and staying on track. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of brian1...@email.com Sent: Thursday, July 29, 2010 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Does anyone have any advice on good study aids, areas of prep to concentrate on, or any test taking strategies that helped them? I have been pouring over the 3rd ed of histotechnology, a self instructional text for months but since I have started to look at the ASCP/ NSH discussion boards im getting the feeling that it is just not enough. Thanks for any help. -Brian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trying to locate metal slide racks for 19 slides
Histonetters, It has been so long since we had to order these slide racks, I am can only hope they are still made/available. Lipshaw(that's digging into the past!) / Shandon / ThermoShandon used to carry them but not seeing them on Thermo Scientific website. What has been found are metal racks with slanted sides, but those are unacceptable. The racks are stainless steel with straight sides, detachable handle, and hold 19(20?) slides in horizontal position. Handles are detachable. I think the last supplier who had these was RA Lamb, now part of Thermo Scientific but not finding the racks on Thermo's website. So far a search has not located straight sided racks for 19(20) slides - only slanted side racks. A supplier would be greatly appreciated. Also, who still carries the 20 slide capacity staining dishes? Thanks, Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reticulum stain wash-offs
Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder, BA, HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
