RE: [Histonet] friable or crumbly O.C.T.
I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to warm up to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen Sent: Friday, August 06, 2010 2:26 PM To: histo...@pathology.swmed.edu Subject: [Histonet] friable or crumbly O.C.T. Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? Here's how it was used. Thanks. We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping. Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Job Alert 8-09-10 Histotech needed in Charlotte, NC
Hi Histonetters!! I hope everyone had a great weekend. I have an exciting new opportunity to tell you about. I am working with a premier client located Charlotte, NC. This is a part time (32 hours per week) permanent position in a full service histology lab. My client is looking for an ASCP certified histotech with at least 2 years of histology experience must be able to meet CLIA requirements to do grossing. (My client does mainly simple grossing, mainly biopsies and excisions). My client offers excellent pay and benefits. If you or anyone you know would like more information please contact me at 866-607-3542 or rel...@earthlink.net Have a Great Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] friable or crumbly O.C.T.
I will 2nd this. When I did neuropathology at a major institution, we froze all frozen sections in an isopentane slurry cooled with LN2. We waited for the OCT to warm to cryostate temps before cutting. If there was time pressure from the surgeons, I used my thumb to warm more quickly, until sections stopped falling apart. We got minimum freeze artifact with this method. For research we used homogenized brain (brain paste) instead of OCT which gave better sections as there was not a change in physical properties you get with OCT vs brain. Bill Blank, MD At 10:40 AM -0400 8/9/10, Della Speranza, Vinnie wrote: I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to warm up to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Precipitate in Processor
Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Precipitate in Processor
Phosphate buffered formalin followed by concentrated alcohol will produce these phosphate salts. To prevent this, formalin should be followed by alcohol of 70% or less. Also, when you change your processing solutions, you can do a water flush (we do first 4 solutions - 2 formalin 2 alcohol) to dissolve any salts that may be built up in the lines. Brandi Higgins HT(ASCP), BS On Mon, Aug 9, 2010 at 12:00 PM, Adrienne Aperghis Kavanagh aaperg...@uspath.com wrote: Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Solutions Hot Histology Job Alert!! Histotech needed in Indianapolis area.
Hi Histonetters! I want to put the word out on another position I am very excited about. I am working with a hospital in the Indianapolis area who is in need of a histotech with at least 5 years of experience for a permanent day shift position. My client offers excellent pay, benefits and relocation assistance. If you or anyone you know might be interested please contact me at rel...@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Re: [Special Stains] Masson's Trichrome
We don't perfom Masson Trichrome but the one-step Gomori version and another rather similar trichrome called SFOG. I use Bouins for one year, better said, when the reagens in the coplin jar goes low, I fill it up again. Our selfmade SFOG solution is used even longer than one year. And the commercial Gomori-solution is used for a half year. Weigert's is made once a week, but the results show that the older solution doesn't work as well. Since nuclei aren't really important in the trichromes, it doesn't matter that much. It's important, that the pH is correct and low enough, to render the solution stable. Our numbers of stained trichromes are about 150 to 200 per year. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sherwood, Margaret Gesendet: Montag, 09. August 2010 16:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (AB) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a set rule when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Precipitate in Processor
That is a phosphate precipitate most likely from your 10%NBF. Try going into a 70% ETOH first followed by higher concentrations. To see for yourself pour 10%NBF into graded alcohols- Precipitate is easy to see with 100% ETOH Jon Henry St. Onge Dako North America Quality Control Supervisor -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh Sent: Monday, August 09, 2010 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate in Processor Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Precipitate in Processor
My guess is that either your 70% wasn't made up properly and was a higher concentration or it's been so long since you've changed the solution that the water is fully saturated with the formalin salts. If it becomes a regular problem, you might consider reducing your first alcohol's concentration to 60% or even 50%. Good luck! Drew On Mon, Aug 9, 2010 at 12:00, Adrienne Aperghis Kavanagh aaperg...@uspath.com wrote: Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Stability of Special Stains
I want to thank everyone who answered my inquiry into the stability of special stains. And a special thanks to Rena Fail, for the link to the University of Rochester's Special Stains manual. It was very helpful. You have answered my questions. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent Grade Alcohol Brands
Hello All, What brands of reagent grade ETOH is everyone using?? Leica is having some manufacturing issues...need some ASAP!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Precipitate in Processor
Thank you all for your quick and helpful responses! I find it strange that we haven't encountered this problem until today, since we have been using the same NBF and alcohols... We usually do the processor hot water flush weekly, on the 1st four stations. I will flush the 1st seven stations and will do it twice to be sure we get out any leftover precipitate. In addition to the flush, I will change the 70% alcohol daily until we receive in another formalin. Thanks again all! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, August 9, 2010 1:03:43 PM Subject: Histonet Digest, Vol 81, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. ca4df32ed505d94bb55e95487d8e984104f...@doaisd5205.state.mt.ads (Andrew Burgeson) 2. Re tunel staining (Steven Weston) 3. Re: [Special Stains] Masson's Trichrome (Sherwood, Margaret ) 4. RE: friable or crumbly O.C.T. (Della Speranza, Vinnie) 5. Re: friable or crumbly O.C.T. (jsjurc...@comcast.net) 6. Anti-IDO (indolamine 2,3-dioxygenase) (Mark Tarango) 7. Re: Re: [Special Stains] Masson's Trichrome (Rena Fail) 8. RELIA Histology Job Alert 8-09-10 Histotech needed in Charlotte, NC (Pam Barker) 9. RE: friable or crumbly O.C.T. (Bill B.) 10. Precipitate in Processor (Adrienne Aperghis Kavanagh) 11. Re: Precipitate in Processor (Brandi Higgins) 12. RELIA Solutions Hot Histology Job Alert!! Histotech needed in Indianapolis area. (Pam Barker) 13. AW: [Histonet] Re: [Special Stains] Masson's Trichrome (Gudrun Lang) 14. RE: Precipitate in Processor (Jon St.Onge) 15. Re: Precipitate in Processor (Drew Meyer) 16. Re: Stability of Special Stains (Sherwood, Margaret ) -- Message: 1 Date: Sun, 08 Aug 2010 13:10:54 -0400 From: Andrew Burgeson nap...@siscom.net Subject: [Histonet] ca4df32ed505d94bb55e95487d8e984104f...@doaisd5205.state.mt.ads To: Histonet@lists.utsouthwestern.edu Message-ID: 4c5ee51e.73.527e.1030580...@siscom.net Content-Type: text/plain; charset=iso-8859-1 I am wondering how much agitation is required in order to achieve the clearing of paraffin from the tissue and slide. IHC systems obviously use adhesive or + slides, aiding in tissue adherence. Too much agitation might take take tissue off. And what about nail fragments or hard tissues in general? Will they survive? I have an article and info regarding this type of deparaffinization and will try to get it into an e-mail. My sense is that it could very well be less efficient and time consuming and that depending on how it is done, could yield very different results. I still think that there's nothing like xylene!!! But obviously soap and water doesnt hurt your liver Interesting method I would like to hear more about people's experiences. -- Message: 2 Date: Mon, 9 Aug 2010 14:32:52 +1000 From: Steven Weston steven.wes...@utas.edu.au Subject: [Histonet] Re tunel staining To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: c885c214.2618%steven.wes...@utas.edu.au Content-Type: text/plain; charset=big5 I recently had an enquiry from one of our post docs who was looking at TUNEL staining and came across this reference in biochemica no 4 (1997)titled ��Fixation of Tissue Sections for TUNEL Combined with Staining for Thymic Epithelial Cell Marker�� You should be able to google it and find the article. Basically it said that after fixation with PFA to improve staining you need to treat with triton and sodium citrate in the cold to expose the antigen again. Below is the suggested protocol for thin cryostat sections. I��m sure it could be modified for thick sections. 1. Cut 4 �gm thin cryosections and mount on polylysine-coated glass slides. 2. Air dry overnight at room temperature and freeze foil- wrapped slides at �V20�XC until use. 3. Apply frozen glass slides directly into a container with 1% buffered paraformaldehyde for 30 min at RT. 4. Rinse swiftly in PBS and immerse in a solution of 1% Triton X100 (v/v) and 1% sodium citrate (w/v) for 2 min at 4�XC. 5. Wash in PBS and incubate with 50 �gl TUNEL reaction mixture (TdT solution with dUTP-FITC solution, 1+9). Convert to enzyme label if desired. 6. Wash with PBS and block with dilution of adequate normal serum. 10. Proceed with desired immunohistochemical labeling. Regards Steve Weston Senior
[Histonet] Leica 2125
Have little Fiscal year money left . Looking for usd Leica 2125 cheep. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reagent Grade Alcohol Brands
What kind of issues? I'm considering switching over to all Surgipath (Leica) products. I'd like to hear more of this. From: kristen arvidson arvidsonkris...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu Date: 08/09/2010 12:05 PM Subject: [Histonet] Reagent Grade Alcohol Brands Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All, What brands of reagent grade ETOH is everyone using?? Leica is having some manufacturing issues...need some ASAP!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reagent Grade Alcohol Brands
We use Protocol from Fisher. Ask them for pricing. We get 70,80,95 and 100% from them. No problems. Bernice Hello All, What brands of reagent grade ETOH is everyone using?? Leica is having some manufacturing issues...need some ASAP!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Bernice Frederick HTL (ASCP) Pathology Core Facility Robert H Lurie Cancer Center Northwestern University Olson Room 8421 Chicago Il 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p63 from Ventana
Hi Everyone, We have been using the p63 (BC4A4) antibody from BioCare for the past 5 years with great results. We use it as a component on our triple stain for prostate. I have been seeing ads on the Ventana website for their p63 and was wondering if anyone else had used their p63 and in what immuno? Any feedback would be greatly appreciated!!! Thanks again! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Anti-IDO (indolamine 2,3-dioxygenase)
Here I am answering my own question. I just remembered where I used to get this antibody. It's from Millipore/Chemicon. Mark On Mon, Aug 9, 2010 at 7:53 AM, Mark Tarango marktara...@gmail.com wrote: Hi Histonet, Does anyone use an Anti-IDO on FFPE tissues? Would you please let me know where you purchase it? Thank you, Mark Tarango ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] correct CPT code
How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] correct CPT code
You need to be more specific about what it is you are doing. If you are looking at imprints or smears intraoperatively then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidec hildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] correct CPT code
This is how I view this also. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Monday, August 09, 2010 2:17 PM To: Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code You need to be more specific about what it is you are doing. If you are looking at imprints or smears intraoperatively then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidec hildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] correct CPT code
Bill, I'm specifically referring to visualization of a renal biopsy under a dissecting scope to see if the specimen is adequate for analysis (i.e. are there glomeruli present?) and examining a ciliary biopsy, again under dissecting microscope, to check for the presence of motile cilia Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: Tench, Bill [mailto:bill.te...@pph.org] Sent: Monday, August 09, 2010 3:17 PM To: Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code You need to be more specific about what it is you are doing. If you are looking at imprints or smears intraoperatively then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidec hildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flat mount eye sections
I have done flat retina sections- which may be what you are being asked to do which is a very specialized procedure even for most research labs. Do you have access to a sliding microtome with a freezing stage? Otherwise I do not know any way to do this. If you are doing flat retina (I have done birds and rats- the rats are very difficult and require a dissecting scope to even prepare them). I did this in a lab 15 years ago but now that I am older and do not see so well, I would use a scope for any species. For birds or rodents, perfuse the animal usually 4%PFA, remove the eye, excise the cornea, iris and lens and being carefully not disturbing the retina remove the vitreous humor and post fixed in 4% at 4oC overnight Transfer to 30% sucrose in PBS at least another 24 hours Dissect the retina from the eyecup and trimmed it with a fresh scalpel blade to flatten it out. I cannot remember the correct term, but pigeon and chicken eyes have a comb structure inside their eyes that must be removed before flattening. It usually took about 4 cuts of varying sizes to get a flat sample. Looks kind of like an uneven flower with cuts from the center out. On a few eyes they had to be cut into 2 pieces to get them flat. On a sliding microtome with freezing stage (or with dry ice on either end of the platform, build a 30% sucrose platform bigger than the eye to be sectioned Then with the blade in place, shave down the platform to make a flat place big enough to mount the retina. Remove the blade for safety reasons for mounting of the retina. Use a wooden block that is super flat covered with saran wrap. Very carefully on one side so there were no wrinkles in the wrap, place the inside of the eye against the saran wrap (with no liquid or it will slide off) and very carefully but quickly wipe the retina onto the prepared platform and let it freeze. Replace the blade from and drop the stage or raise the blade (depending on the microtome ) and carefully section the retina. On a pigeon we usually got at best 3 30u sections but usually did not get the retina quite flat and got 4 sections. The sections are collected in PBS and stained in micro centrifuge tubes for various antibodies then mounted on slides after staining.. Good luck Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2009 San Diego, CA 92093 858 534 7438 Date: Wed, 04 Aug 2010 10:57:02 -0700 From: sgoe...@xbiotech.com Subject: [Histonet] Flat Mount To: histonet@lists.utsouthwestern.edu Hello all, I am being asked to do immunostaining on fla eyes. First, what is a flat mount and does an on how to make one? Second, what is the methodol stains on these? Do you have to fix them, cut them,done this and need some help. Thanks guys!! Sarah Goebel, B.A., HT (ASCP) Histotechnician [DEL: XBiot 8201 East Riverside Dr. Austin, Texas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] p63 from Ventana
I've been told by a Biocare Salesperson that the BC4A4 clone is the same exact clone as 4A4. The BC in front of 4A4 just means Biocare. I don't think that Ventana sells a concentrate of this antibody. You'd want a concentrate for your PIN4 so you're probably better off sticking with your current antibody. Mark On Mon, Aug 9, 2010 at 11:46 AM, Adrienne Aperghis Kavanagh aaperg...@uspath.com wrote: Hi Everyone, We have been using the p63 (BC4A4) antibody from BioCare for the past 5 years with great results. We use it as a component on our triple stain for prostate. I have been seeing ads on the Ventana website for their p63 and was wondering if anyone else had used their p63 and in what immuno? Any feedback would be greatly appreciated!!! Thanks again! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] correct CPT code
OK, so that's a different story (and we look at renal cores for glomeruli as well). I believe the correct code for that is 88329--intraoperative consultation. It is not a frozen section so that code, 88331, does not apply. Again, I believe that to be paid, this activity must be performed by a pathologist. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: Houston, Ronald [mailto:ronald.hous...@nationwidechildrens.org] Sent: Monday, August 09, 2010 12:57 PM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code Bill, I'm specifically referring to visualization of a renal biopsy under a dissecting scope to see if the specimen is adequate for analysis (i.e. are there glomeruli present?) and examining a ciliary biopsy, again under dissecting microscope, to check for the presence of motile cilia Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: Tench, Bill [mailto:bill.te...@pph.org] Sent: Monday, August 09, 2010 3:17 PM To: Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code You need to be more specific about what it is you are doing. If you are looking at imprints or smears intraoperatively then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidec hildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content
[Histonet] closure of EM lab
We are closing our EM service and have the following equipment to surplus: Philips CM 100 Electron Microscope w/ Haskris Water chiller and Jun Air Compressor. Ted Pella Nitrogen Burst Negative Developing Tank Durst Laborator S-45 EM Enlarger w/ lens for 35 mm, 3 x 4 and 2.25 x 2.25 sheet film Dessicator w/ Vacuum Pump BEEM Negative Dryer Oven Penetron Rotary Shaker Sorvall GKM Glass Knife Maker We have a Leica UC6 ultramicrotome that may be surplused; may be able to trade for current model, good rotary microtome. Most of the equipment was purchased in the late 78 / 79 and has been carefully cared for and maintained. The Philips CM 100 was installed in 11/95 and has been under continuous service with FEI / Philips. The Leica microtome is only 2 / 3 years old. Our hospital follows the surplus guidelines established by the Shands Healthcare System. I am trying to get the word out to other EM users and will gladly put you in contact with our surplus co-ordinator. Or work with you to see the equipment which is still set up within our department. Please pass on to others that may have an interest. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
