[Histonet] Paraffin
Hello, Just wanted to know if anyone has tried Henry's Wonder Wax Xtreme Paraffin w/DMSO made by Mercedes Medical. What were your expirences with it? Is it comprable to McCormick's Paraplast Xtra? Thanks in advance, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide label printers
Hi gang... I am hoping that someone out there can lend me some assistance. We currently are using paper slide labels( 0.875 in by 0.875 in) on our Pathology slides, an old label printer (Genicom Model 1025) and Meditech Magic software. Our dilemma is this... we no longer can find the ink ribbons for our printer. Is there anyone out there who is using the same system? In about 1 1/2 to 2 years, we are going to be changing to a new software (possibly McKesson), so we are not looking to really buy anything expensive at this time. We just really need to continue using the paper labels until we have the change over. Thanks for your help. Valerie Hannen, MLT (ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana ES labels
Hi, I am posting this for a colleague of mineShe is in the middle of a project and found out that she can no longer order Ventana ES labels. She wants to know if anyone got rid of their ES stainer but still has some labels in their lab that they are looking to get rid ofor if anyone knows where she might find some labels... Any help would be greatly appreciated. Please respond to my e-mail and I will get the information to her since she is not on the Histonet... Thanks, Vicki Gauch AMCH Albany, NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Teaching Opportunity
Keiser University in Orlando Florida is seeking an adjunct faculty member to teach evening classes for their Histotechnology program. Candidates must have an Associate's degree and HT certification. If interested please contact: Schaundra Walton BS, HTL(ASCP) Histotechnology Program Director Keiser University 5600 Lake Underhill Rd. Orlando, FL 32807 407-273-5800 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] inverted meniscus
Today while perfroming an xylene purity check after recyling xylene on a CBG recycler, I noticed that the meniscus between the DH2O and Xylene is inverted...that is convex instead of concave. I have done this check many times( you add exactly 15 mls of water to exactly 85 mls xylene, invert and allow to settle out and observe the meniscus at the separation site for changes in volume of water) and have never seen it invert. The glass cyclinder was clean , we repeated three times and still same results. Anyone have a clue as to why? CBG did not have an answer . thanks Mary F Benoit MT(ASCP) The Pathology Laboratory Lake Charles, LA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Tape coverslipper
Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] inverted meniscus
Maybe it twisted it's knee while out playing touch football. From: Mary Benoit mbp...@yahoo.com To: histonet@lists.utsouthwestern.edu Date: 08/11/2010 01:21 PM Subject: [Histonet] inverted meniscus Sent by: histonet-boun...@lists.utsouthwestern.edu Today while perfroming an xylene purity check after recyling xylene on a CBG recycler, I noticed that the meniscus between the DH2O and Xylene is inverted...that is convex instead of concave. I have done this check many times( you add exactly 15 mls of water to exactly 85 mls xylene, invert and allow to settle out and observe the meniscus at the separation site for changes in volume of water) and have never seen it invert. The glass cyclinder was clean , we repeated three times and still same results. Anyone have a clue as to why? CBG did not have an answer . thanks Mary F Benoit MT(ASCP) The Pathology Laboratory Lake Charles, LA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Tape coverslipper
OMG. This problem was ever present in our lab for years, until I figured out that ANY minute trace of moisture, water, eosin in the last absolute alcohol would interfere with the activation of the adhesive in the tape, and create this brown artefact. We religiously change our absolute alcohols on the autostainer so the last alcohol is perfectly clear of any hint of eosin, and the artefact has disappeared from our tape-coverslipped slides.The Sakura rep couldn't help me either, and told me they had NEVER seen this problem before. Love it. Keep your last 100% PRISTINE, and the artefact will be eliminated. I promise. Jackie O' From: Nails, Felton flna...@texaschildrens.org To: 'Gauch, Vicki' gau...@mail.amc.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 08/11/2010 01:45 PM Subject: [Histonet] Sakura Tape coverslipper Sent by: histonet-boun...@lists.utsouthwestern.edu Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] purple spots
Hi All, I recently did an Oil-Red-O stain using my usual procedure with formaldehyde vapor fixation, Harris hematoxylin, and finally Oil-Red-O. I was staining some frozen diseased aortic mouse tissue. This time the stain resulted in some dense purple spots in the tissue. I had not seen this before. I have posted pictures on the www.histonet.org website. The files are named low mag 2c, 2b, 2d, and 2e. The last three are a higher magnification of the first picture. I was hoping someone can tell me what these spots are. I suspect that they are simply necrotic tissue, but I would like the opinion of experts. Thanks for taking a look! Kathy Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fragmentation on core bone marrow biopsies
We are looking at the cause of fragmentation on our bone marrow core biopsies. We are reviewing fixative times and decalcification times. Has anybody else experienced fragmentation problems? If so how did you eliminate the problem? What other variables do I need to look at that might be the cause? Thanks for the suggestions in advance. We do use an AZF fixative and a gentle decal solution called Rapid-cal. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica ST5010/ST5020 vs Ventana Symphony
For those of you who currently use or have used any of these instruments: Why did you chose it and how do you like? If you've used both, which do you like better and why or why not? We're currently manually testing. We're considering moving our lab into this century and need a little advice. Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAP
Dear friends, does anyone have rapid PAP(cytostain) preparation,for rapid PAP staining. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals chennai India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 80, Issue 31
Dear Friends, Does anyone have rehydration technique of air dried smear which can be stained for pap. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals -chennai India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
