[Histonet] xylene in an unventilated area
Hello all. Cytology was told that the xylene substitute the= y were using for their Surepath paps was causing staining/artifact issues. nbsp; They want to switch to using xylene in their area, but it is unventil= ated. Because of various reasons, their process can not be moved.nbs= p; They are asking me for advice about what to do for fume control. I= was wondering if a good downdraft unit would be enough to handle the xylen= e fumes. They will have maybe 400 mL of xylene uncovered while covers= lipping their paps. Does anyone else have a similar set up? The= y share their area with micro and core lab and others are complaining about= fumes even though they have borrowed a mini-hood from us. Badge resu= lts are still pending. Cathy Crumpton HT(ASC= P), Histology Lead Tuality Community Hospital Hillsboro, OR 97123 = (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] xylene in an unventilated area
Try to get a fume hood; it could be one movible over the counter, but needs to have a good charcoal filter. René J. --- On Mon, 8/16/10, cathy.crump...@tuality.org cathy.crump...@tuality.org wrote: From: cathy.crump...@tuality.org cathy.crump...@tuality.org Subject: [Histonet] xylene in an unventilated area To: histonet@lists.utsouthwestern.edu Date: Monday, August 16, 2010, 11:41 AM Hello all. Cytology was told that the xylene substitute the= y were using for their Surepath paps was causing staining/artifact issues. nbsp; They want to switch to using xylene in their area, but it is unventil= ated. Because of various reasons, their process can not be moved.nbs= p; They are asking me for advice about what to do for fume control. I= was wondering if a good downdraft unit would be enough to handle the xylen= e fumes. They will have maybe 400 mL of xylene uncovered while covers= lipping their paps. Does anyone else have a similar set up? The= y share their area with micro and core lab and others are complaining about= fumes even though they have borrowed a mini-hood from us. Badge resu= lts are still pending. Cathy Crumpton HT(ASC= P), Histology Lead Tuality Community Hospital Hillsboro, OR 97123 = (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nail Problems /Equipment
I am replying to both posts, getting the section of the nail used to be the toughest thing to do,now that I use the Sodium Hydroxide soak it seems that I have less problems keeping the sections on. To help that issue, after cutting and drying in the oven for 10 minutes, I put the slide in a Coplin jar with about 1cc of formaldehyde.Put the lid on loosely . Leave this in the oven 1-2- ours and then stain. About the instruments , we have a Ventana Benchmark XT. It works fine , we just upgraded to the Ultra for continuous load and do not have room for both in the Lab. We do still use it for routine IHC, and was last serviced in February this year. (Embedded image moved to file: pic17752.jpg)___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p63
Histonet, I had read about a week ago about someone asking about the rights to sell p63. I did some research and hope this helps: http://www.histobob.com/?q=node/121 Thanks, Ian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal base molds
Thanks to all who answered my request. I checked out EMS and Ted Pella, but found the best size/best price right in my own backyard--Brain Research Lab in Newton, MA. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, August 12, 2010 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] metal base molds The following link has larger molds, maybe one of them will work. http://www.brainresearchlab.com/productinform.html Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 Message: 20 Date: Thu, 12 Aug 2010 11:51:37 -0400 From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] metal base molds for paraffin embedding To: histonet@lists.utsouthwestern.edu Message-ID: 073ae2bea1c2ba4a8837ab6c4b943d9703e24...@phsxmb30.partners.org Content-Type: text/plain; charset=us-ascii To all: We need to order metal base molds for paraffin embedding. We need a size @45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we ordered them). I have looked in several catalogs but can only find a depth of 5mm. The depth is the important part. If someone knows where I can order these, I would appreciate it. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] attention equipment resellers/refurbishers
I have a 6 foot external exhaust fume hood with blower that I need to find a home for. It has a fairly large sink in the left side of the countertop and 1/2 of the base cabinet is for flammable storage. If interested please contact me personally at camay...@gmail.com. I can send pictures to interested parties. -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] oh my god-j
hey my god : I bought 5 products Apple iPhone 4G HD 16GB Black website: dhsellso.com ,to my surprise! it's genuine. much cheaper.You can check it! Hope all is going well for you. 8 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QC on stained slides
You have included all the criteria; fixation, processing, embedding, microtomy, staining, coverslipping and labeling. If you were able to submit slides to CAP under their HQIP program, you would get graded evaluations. Short of that, could you perhaps send out duplicate slides to another local lab, or sister hospital, for peer review? You could offer to exchange slides on a twice yearly basis, since they, too, may be looking for additional quality control. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, August 02, 2010 2:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QC on stained slides Hi all As part of a self assessment programme conducted by my employer, and related to my performance review and salary adjustment, I need to determine the criteria of what makes a stained slide acceptable or unacceptable. I was wondering if anyone out there had a checklist that they would be willing to share, that i could perhaps adapt. I realise that the easiest would be to send slides out for external control, but in this case it is not feasible. What I put together is this: - Quality of decalcification, processing, infiltration - Quality of sections (no wrinkles, missing bits, scores etc) - Entire representation of tissue area - staining pattern as expected according to protocol - coverslipped without bubbles or other inclusions - labelled neatly and correctly but, the question inmy mind is what would be the criteria that would make a slide merely adequate or truely outstanding? PLease help thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Janella Seaton/WLGORE is out of the office. (returning 08/23/2010)
I am out of the office until 08/23/2010. I will respond to your message when I return. = Any histology-related requests or questions can be sent to: histol...@= wlgore.com. Note: This is an automated re= sponse to your message Histonet Diges t, Vol 81, Issue 19= sent on 8/16/2010 10:07:34 AM. This is the only notification= you will receive while this person is away.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] xylene in an unventilated area
I just recently did a lot of research into the different hoods. I decided the down draft was not going to work for us since we like to place absorbant towels down to catch the xylene while coverslipping. I decided to purchase one that a high CFM (Air flow rate), low decibles and a monitor on the filter to let one know when the filter was saturated and needed to be changed. And I wanted the sash that comes down in front of your face to be glass, so that we could clean it periodically with xylene to get splashes of mounting media off. For the money PurAir was very good... body was plastic and the clear part that is lowered while in use was plastic. It had good CFM, good decible level and a monitor for filter saturation.The LabConco is stainless steel and glass, with good CFM, decible level and a monitor for filter saturation. The problem with the little Shandon is that the CFM is about 30 at max when the sash is lowered. Newer ones, such as the PurAir and Labconco have about 120+ CFM. And the Shandon has no monitor for filter saturation. The PurAir had the footprint needed for cytology, which is very small in my area. There is another company that was good, but my brain cells cannot recall the info. If anyone wants more info, please e-mail me. In a message dated 8/16/2010 12:01:08 P.M. Central Daylight Time, rjbu...@yahoo.com writes: Try to get a fume hood; it could be one movible over the counter, but needs to have a good charcoal filter. René J. --- On Mon, 8/16/10, cathy.crump...@tuality.org cathy.crump...@tuality.org wrote: From: cathy.crump...@tuality.org cathy.crump...@tuality.org Subject: [Histonet] xylene in an unventilated area To: histonet@lists.utsouthwestern.edu Date: Monday, August 16, 2010, 11:41 AM Hello all. Cytology was told that the xylene substitute the= y were using for their Surepath paps was causing staining/artifact issues. nbsp; They want to switch to using xylene in their area, but it is unventil= ated. Because of various reasons, their process can not be moved.nbs= p; They are asking me for advice about what to do for fume control. I= was wondering if a good downdraft unit would be enough to handle the xylen= e fumes. They will have maybe 400 mL of xylene uncovered while covers= lipping their paps. Does anyone else have a similar set up? The= y share their area with micro and core lab and others are complaining about= fumes even though they have borrowed a mini-hood from us. Badge resu= lts are still pending. Cathy Crumpton HT(ASC= P), Histology Lead Tuality Community Hospital Hillsboro, OR 97123 = (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
