[Histonet] Thank you!
Hi Histonet, I just would like to thank everyone who responded to my question about Warthin-Starry stain. Your help is very appreciated! Chakib ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] melted paraffin storage and dispensing
I do histology in a small research lab. We use the tissue processor and embedding station of a nearby lab to process specimens. I would like to switch to manual in-lab processing of some our more delicate samples like mouse embryos. I am wondering what histonetters use to store their melted paraffin when you don't have access to an embedding station. I have an appropriate incubator, but what kind of container should be used? I have seen metal paraffin pails used elsewhere - anyone know where to purchase these? Is a metal container necessary or will a plastic or glass beaker work? Are there any problems with storing paraffin (Paraplast Plus) between uses in the liquid state, or should I remelt it each time I want to embed samples? Anything else I should be concerned about that I am overlooking? Thanks, Andrea Marion Graduate Student University of Illinois at Chicago amario3 |at| uic |dot| edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tonsils
We receive and process all tonsils. We charge 88304. Brandi Higgins, BS, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Attention new equipment distributors
Can you give me a ball park figure for a new paraffin embedding center? I have a friend setting up a new lab and are working on their budget. They need a cost of a unit equivalent to the Shandon Histocentre that has the electric forceps. About 7 years ago I paid about $7000 for a unit such as this but of course time and costs have changed things. Please respond off line to camay...@gmail.com Thanks in advance, -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] accu-edge low profile microtome blades
We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: accu-edge low profile microtome blades
The oil serves a valuable purpose and shopuld be found on all blades of high quality. It protects against oxidation and allows the blades to be easily separated from one another, so the oil has always been there. I have personally found no advantage to wiping or not wiping the oil from the slides. My 2cents - Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, September 01, 2010 1:27 PM To: Laurie Colbert; Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen sections missing centers
Hello, I have been asked to to prepare frozen sections of fish livers that have been stored in RNAlater Solution at -80C. Tissues (about 10 um square x 5 um thick) were embedded in OCT, frozen on dry ice, and sectioned at 6 um. My problem is that when I cut a section, the center of the tissue is missing! Approximately 1 mm of tissue around the edges seems to section just fine, but then there is just a gaping hole in the center as if the tissue is receding while it is being cut. I've tried sectioning at various temperatures (-20C, -16C, and -12C) and thickness (4um-8um). Any help will be greatly appreciated, Carla Conway Western Fisheries Research Center Seattle, WA ph: 206-526-6282 ext. 242 cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome Blades
The best blades we've used so far are from Mercedes Medical - MER DMBHPGP - and are priced well below some competitors blades. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] accu-edge low profile microtome blades
About 1/2 of my tech.'s wipe the oil off; otherwise it causes the first 2-3 ribbons to curl off the knife, rather than forming a nice ribbon. Sandy Harrison Histology Supervisor Minneapolis VA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, September 01, 2010 1:27 PM To: Laurie Colbert; Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
