Re: [Histonet] filtering cytology stains
Hi - why not look at something like a millipore (millivex) syringe filter? This website has the application choice on it - or perhaps speak to your Millipore rep? http://www.millipore.com/techpublications/tech1/pb1951en00 On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins brandihigg...@gmail.comwrote: Hello All, I was wondering how you are filtering your cytology stains. We are a relatively small lab, so we have been gravity filtering our cytology stains, but as we are getting busier with a larger volume of slides, especially fna's this is becoming a very time consuming process. I think vacuum filtration would be almost as lengthy a process. It was suggested that we look into getting some 200ml syringes that can come with an attached filter to suck in and pump out the fluid for faster filtration...is anyone using such a process? If so, do you have product names/numbers for the filters or the syringes. If anyone has another method they are using I would like to hear any suggestions. Thanks in advance for you input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PMS2
We used the same antibody but then at 1:1200, retrieved with PH9 (PT module buffer 4, thermo) on a labvision stainer. We had beautiful results. Now, we switched to Ventana Benchmark XT (1:50, 30' cc1 + amplification) and our results are quite poor for PMS2: many cytoplasmatic staining. So if anybody has a good protocol for the Benchmark XT, I am very interested. Regards, Willem Hoekert OLVG, Amsterdam Van: histonet-boun...@lists.utsouthwestern.edu namens Dana Settembre Verzonden: wo 25-8-2010 12:12 Aan: dianar...@aol.com; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] PMS2 Diane, When I was doing PMS2 I was using BD's mouse antibody @ 1:10 Retreived with Dako's TRS in a steamer for 40 min, incubated the antibody for 60 minutes and I used a labelled polymer for the detection (Dako's Envision + Mouse) It was difficult to work up. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJUSA dianar...@aol.com 08/24/10 8:47 PM Can anyone share their protocol for PMS2? I just keep getting background staining. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great HT Position in Colorado
Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrin...@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great Position in Irving , Tx
Nueterra, a leading developer and manager of physician-owned surgery centers and specialty hospitals has an immediate opportunity available for a Histotechnician. Great opportunity for a Histotechnician in a brand new laboratory! Nueterra Pathology Services in Irving, TX is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers a competitive salary, medical/dental insurance, 401K plan, and vacation/sick leave. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email at mh...@carisdx.com mailto:mh...@carisdx.com . Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
How do you handle this if you have no one that comes in on the weekend? Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] ANP. 23075
I guess there's nothing you can do. -Original Message- From: Kaye Ryan [mailto:kr...@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health
RE: [Histonet] ANP. 23075
By using a thermometer that records high and low temperatures, you can see if your fridge or freezer went out-of-range over the weekend. This satisfies CAP Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:27 AM To: Kaye Ryan; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I guess there's nothing you can do. -Original Message- From: Kaye Ryan [mailto:kr...@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential,
RE: [Histonet] ANP. 23075
By using a thermometer that records high and low temperatures, you can see if your fridge or freezer went out-of-range over the weekend. This satisfies CAP and, should have mentioned it earlier, JCAHO Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:27 AM To: Kaye Ryan; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I guess there's nothing you can do. -Original Message- From: Kaye Ryan [mailto:kr...@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is
RE: [Histonet] ANP. 23075
We use a thermometer that will indicate to us if the temperature went high over the weekend. There is a little group of pellets at one end that will drop to the other end if the temperature is out of range. We call them Visual Indicator. You can either buy them through one of the major distributers. They come for freezers or refrigerators. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan [kr...@nfderm.com] Sent: Thursday, September 16, 2010 10:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled
RE: [Histonet] filtering cytology stains
Hi Brandi, Depending on your stain line set up and the number of FNA's that you are doing, you may want to consider having multiple stain set up's that you can switch to in a hurry. This works well if you only filter stains and change alcohols. You can keep several boats or the smaller manual staining dishes filled with Hematoxlyn, OG and EA. Even better and quicker if you use the EA/OG combo stain. The stain dishes or boats that were used for positive FNA's can be tagged with a colored sticker and set aside to be filtered all together. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Thursday, September 16, 2010 3:16 AM To: Brandi Higgins; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] filtering cytology stains Hi - why not look at something like a millipore (millivex) syringe filter? This website has the application choice on it - or perhaps speak to your Millipore rep? http://www.millipore.com/techpublications/tech1/pb1951en00 On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins brandihigg...@gmail.comwrote: Hello All, I was wondering how you are filtering your cytology stains. We are a relatively small lab, so we have been gravity filtering our cytology stains, but as we are getting busier with a larger volume of slides, especially fna's this is becoming a very time consuming process. I think vacuum filtration would be almost as lengthy a process. It was suggested that we look into getting some 200ml syringes that can come with an attached filter to suck in and pump out the fluid for faster filtration...is anyone using such a process? If so, do you have product names/numbers for the filters or the syringes. If anyone has another method they are using I would like to hear any suggestions. Thanks in advance for you input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
We use a continuous-read thermometer to monitor the temp in our block/slide storage area. They probably make these for refrigerators. -Original Message- From: McMahon, Loralee A [mailto:loralee_mcma...@urmc.rochester.edu] Sent: Thursday, September 16, 2010 7:54 AM To: Kaye Ryan; Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 We use a thermometer that will indicate to us if the temperature went high over the weekend. There is a little group of pellets at one end that will drop to the other end if the temperature is out of range. We call them Visual Indicator. You can either buy them through one of the major distributers. They come for freezers or refrigerators. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan [kr...@nfderm.com] Sent: Thursday, September 16, 2010 10:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 82, Issue 20
Hello my friends in histonet world, I'd like to offer my insight into the weekend/holiday temp.(refrigerator) recording issue. I use a traceable thermometer (Fisher Scientific) that is very simple to use and very reliable. It records the daily temp. and the weekend min./max. temp. on Friday we press and hold the memory clear button for a few seconds and on Monday you can clearly see the daily temp. and the w/e min./max. temp. I hope this helps, any questions please feels free to contact mealbert.santi...@uphs.upenn.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, September 16, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 82, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Ventana Symphony (Clare Thornton) 2. NBF fixation and long term storage (Lewis, Patrick) 3. Re: NBF fixation and long term storage (Rene J Buesa) 4. Re: Problem with liver fixation (Matthew Lunetta) 5. drying ovens (Perry, Margaret) 6. ANP 11605 11610 (Sara Baldwin/mhhcc.org) 7. RE: ANP. 23075 (Laurie Colbert) 8. Lab Supervisor/Manager Position in Orange County CA (J Puma) 9. Re: transporting sectioned tissues (Teresa Iglesias) 10. Help! Curled cryosections (Teresa Iglesias) 11. Leica-Surgipath EM Paraffin Issue (Sandra Cheasty) 12. Re: Help! Curled cryosections (Teresa Iglesias) 13. Picrosirius Red Staining of mixed archaeological residues. [SEC=UNCLASSIFIED] (Birgitta Stephenson) 14. Re: filtering cytology stains (louise renton) 15. RE: PMS2 (Hoekert, W.E.J.) 16. RE: ANP. 23075 (Rathborne, Toni) 17. Great Position in Irving , Tx (Hale, Meredith) 18. Great HT Position in Colorado (Hale, Meredith) 19. RE: ANP. 23075 (Laurie Colbert) 20. RE: ANP. 23075 (Kaye Ryan) 21. RE: ANP. 23075 (Laurie Colbert) -- Message: 1 Date: Wed, 15 Sep 2010 13:15:41 -0400 From: Clare Thornton cthorn...@dahlchase.com Subject: [Histonet] Ventana Symphony To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: c9d78ffc9d668b4cbea4405f84697504f801d1d...@iris.dahlchase.net Content-Type: text/plain; charset=us-ascii Hello all, I have some questions for other Symphony users out there. Can someone please contact me off-list if you don't mind answering some questions for me? Thanks much! Clare Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -- Message: 2 Date: Wed, 15 Sep 2010 10:53:41 -0700 From: Lewis, Patrick patrick.le...@seattlechildrens.org Subject: [Histonet] NBF fixation and long term storage To: Histonet@lists.utsouthwestern.edu Message-ID: 7ea5752b2903b143a5b845dea87d5d1c05ac2...@s107.childrens.sea.kids Content-Type: text/plain; charset=us-ascii I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period. (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh. And using 70% Etoh as long term storage of tissue parts. The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh. I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it. Anyone storing research tissues has a suggestion about the best way to store them when space
[Histonet] Cutting, Processing, etc
Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is freezy spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Losing frozen sections
Hello again, Besides my C3 and C5b-9 question I was wondering if anyone has ideas as to why I might be losing my frozen sections during IHC. I'm placing 5u sections on charged slides, air drying overnight, fixing in chilled acetone, air drying again, and proceeding with the protocol, which recently has been an ABC method. Slides I don't use get stored in a -70 freezer. I do these by hand. Could I just be rinsing too vigorously? Sometimes I look at these during the procedure and they look fine, but inevitably many of the sections look horrible- torn and missing tissue, by the time they are coverslipped. Hints and suggestions anyone? Thank you in advance, Dolores The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
A cheap solution for the weekend is to get some electronic thermometers that record the high and low (Fisher has certified electronic thermometers pretty cheap). Then for the weekend you can document what the high and low was for that time period. Also, for critical reagents like antibodies, a chart recorder gives more information. And an alarm is helpful if it actually breaks down. There are temperature-monitoring software/hardware packages that you can put on your equipment that will record all instruments at once and, when connected to the internet, will call or email you if something goes out of range. They start as low a $1,500. You can also get temperature data loggers. They look like small batteries. You just put them in the place you want to log a temperature and they log the temp at intervals you program them for - like every minute, hour, whatever. Then you put them in a reader and download the info to a software program that allows you to make graphs. They are also great for logging temps when shipping items that are temperature sensitive. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under
[Histonet] RE: Cutting, Processing, etc
what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is freezy spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Region II Special Invitation
YOU ARE INVITED!!! For those of you from Region II, who will be attending the NSH S/C in Seattle Sept 24 -30th. I would like to invite you to a special meeting for Virginia, W. Virginia, Washington, D.C.members and any others interested, from Region II...on re-establishing the By-laws for the Virgina Society to complete making it a fully active Society and electing officers for the BOD in VA. The meeting will be held on Saturday 5:00- 5:30 pm, directly following the Region II meeting at the convention Center. Please check your program for the room #. I look forward to seeing all of our Region members, and talking with you about establishing regional goals for the next two years. What is it that you need and what is it that we should be accomplishing? This is your region and you are in the driver seat...but, only if you attend and let me hear your voice. Lets set goals together! I would also like to invite members from our region to attend the NSH House of Delagate, to be held on Wednesday Sept 29th at 6pm (check program). Come and sit in the gallary to learn about how NSH works and how your ideas are incorporated in future plans for the Society. Always wonder what they Board of Drectors, Regional Directors and Delegates actually do? Come and sit in the gallary at the 36th NSH House of Delegate to see and learnand truly hear NSH in Action! C. Barone, Region II Director ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Path Course
Dear colleagues,
We just finalized the schedule for our fifth annual course. Many of you have
already attended our previous courses. Feel free to forward this email to
any interested colleagues.
Also, exhibitors are welcome for sponsorship opportunities. We have a
limited room this year, based on first come-first serve basis.
All information about schedule, location, time, faculty, exhibitors
prospectus, contact info, registration form, are available and downloadable
online from the course's website at
www.pathlearning.com
Thanks,
Hadi
Hadi Yaziji, MD I Medical Director
Vitro Molecular Laboratories I www.vitromolecular.com
7480 SW 40th Street, Suite 700 Miami, FL 33155
Tel 305 267 7979 I Fax 786 513 0175
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
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[Histonet] HSV mAb
Is anyone using a commercially-available monoclonal antibody to Herpes Simplex Virus (for immunohistochemistry) that works well on formalin-fixed, paraffin-embedded tissue? I have not been happy with my polyclonal cocktail recently; too much nonspecific staining. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Surgipath print labeler
Does anyone have a used Surgipath print labeler for sale Thanks, Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 53706 USA tel: 608-262-8533 fax: 608-262-2824 email: plamb...@wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] what is happening to our field?
From: Nails, Felton flna...@texaschildrens.org Subject: [Histonet] RE: Cutting, Processing, etc To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: c1fe5960057c084ca389ce9777906290875ea...@tcdmsg01.ad.texaschildrenshosp ital.org Content-Type: text/plain; charset=us-ascii what is happening to our field?? -I agree! I was told it's a waste of time to change your blade and/or put your blocks in/on water before cutting. My mouth dropped to the ground. This is NOT what I was taught at school - and some of these procedures are killing me! I just want other professionals to confirm what I've already told them...thank you so much for all your private emails! Amy Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA in Ohio
Once again, would the recruiter for the PA in Ohio please contact me again. I forwarded your last email then deleted everything. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alabama Histology Position
FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma HE Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfa...@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Outstanding Histotechnologist Position in FL!!
Good Afternoon Everyone, Our team at Personify has uncovered an extremely strong lab based Histotechnologist position in Leesburg FL. The position offers a very competitive salary and package. Please contact me directly to learn more. Have a great day!!! Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 http://www.personifysearch.com/ www.personifysearch.com lab based in Leesburg, FL TITLE: Histotechnologist II LOCATION: SUMMARY OF PURPOSE: Reports to the Histology Manager and is responsible for processing surgical specimens by performing various Histology procedures, including grossing, chemical fixation, embedding, microtomy, and staining of tissues and fluids. ESSENTIAL FUNCTIONS: 1. Receives and logs specimens into the Anatomic Pathology computer system. 2. Assess quality and appropriateness of specimens received and takes appropriate action if acceptable standard is not met. 3. Performs embedding, microtomy and staining of Histology and Cytology Specimens to meet established productivity and quality standards. 4. Perform gross description on small biopsies. 5. Maintains neat, organized and legible written records 6. Maintains a clean organized work area 7. Maintains appropriate supply inventory at assigned area. Performs supply inventory. 8. Performs routine and special Histology staining procedures accurately and assists in recognition and resolution of potential problems. 9. Files a variety of Histology materials, including but not limited to slides and blocks. 10. Retains a variety of materials including slides, blocks, specimens, requisitions and reports for the appropriate amount of time to comply with inspecting agencies. 11. Follows specialized staining procedures. 12. Participates in in-service training as required. 13. Participates in continuing education as required. 14. Performs Cytology processing as necessary. 15. Prioritizes and reorganizes workload in response to emergency procedures. 16. Adheres to departmental policies and procedures. 17. Demonstrates flexibility in work schedule to include weekends and holidays. 18. Performs required maintenance on all Histology equipment. 19. Documents special stain Q.C. and notifies a level II or III Histotech when Q.C. parameters are not met. 20. Demonstrate professionalism as evidenced by the cooperative attitude of peers and associated staff. 21. Performs minor Histology procedures in conjunction with other Histology personnel such as specimen registration, specimen disposal, filing slides and completing appropriate paperwork for consults. 22. Demonstrates commitment to our mission and Customer Service by adhering to all facets of the Laboratory policies and procedures. 23. Performs other duties as assigned. POSITION REQUIREMENTS: Education and formal training: AS Degree required License: Certification by the Board of Registry of American Society of Clinical Pathologists (ASCP) required. Experience: At least 1 year work experience performing the essential functions of a Histotechnologist. Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 http://www.personifysearch.com/ www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] clarification; slide printer
I am looking for a used Surgipath slide printer to buy. Thanks, Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 53706 USA tel: 608-262-8533 fax: 608-262-2824 email: plamb...@wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin Incidental Spill vs a Release
I trying to see if there is an industry standard for pathology labs regarding formalin spills. Can you tell me what volume of a formalin spill is considered an incidental spill that can be cleaned up by staff versus the volume of formalin that would constitute a Release requiring a Haz Mat team or a responder (such as a fire department) from outside of the hospital or lab to clean it up. I will appreciate seeing how this is handled in your institution. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting, Processing, etc
Well, to answer you questions in order base on my experience in most of the labs I have worked in, except one: 1) after embedding we face in all our blocks. as we face them in, we place the blocks on a pan of melting ice to a) chill them and b) allow the tissue to absorb a bit of moisture. all tissues are treated the same, keeping in mind that some tissue may need a little longer to soak on ice pan than others, but not much. 2) a new blade is always used for taking the keeper sections. 3) except for one lab, freezy spray has never been used. too easy to over chill the block and cause freezing artifacts that way. which, oddly enough, does not happen on melting ice. 4) even though you did not ask, I will also say, that the slides when cut are placed standing up for a few minutes to properly drain before applying any heat. if the slides are not allowed to drain before placing in the oven and stained (this goes for routine HE as well as immuno) the sections will not adhere to the slides properly and will be prone to floating off/fragmenting during staining. It should also be noted that if all slides are allowed to dry thourouly before heat and staining there is little to no need for adding adhesive to the water bath. Hope this helps. Catherine On Thu, Sep 16, 2010 at 11:38 AM, Senn, Amy R ars...@hsh.org wrote: Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is freezy spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cutting, Processing, etc
DON'T get me started... And I have only been practicing 9 years! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Thu 9/16/2010 11:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cutting, Processing, etc what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette Labeler
I work in a small lab and process approx 150 cassettes a day. We currently use a chemical resistant pen that works great. We write the number on top and the patient initials on the side. We are considering getting a cassette labeler. Is it really worth the expense for 150 blocks a day? Is it possible to enter information on the side of the cassette? Can you share some of your experience with different ones with me. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
