Re: [Histonet] Blade sharpening service
Delaware do ours and they're cheaper than leica On Mon, Sep 27, 2010 at 9:47 PM, Collette, Nicole M. collet...@llnl.govwrote: Hello, esteemed colleagues, I am in need of a service to have my 16cm tungsten carbide Profile D (a.k.a. Sweeney Todd) blades resharpened. Can anyone recommend a service that is relatively accessible to the West coast? I looked in the archives and couldn't find any recent posts, and my Googling has been unproductive. I'd rather not send them back to Leica in Germany to have them sharpened if I don't have to. Thanks for all your help! Sincerely, Nicole Collette UC Berkeley/Lawrence Livermore National Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gliotic plaques etc
Hello, I'm interested in quantifying gliotic plaques, tangles and amyloid plaques in 5 um thick wax sections (fixed in Bouins). I'm planning on using Toluidine Blue for the amyloid and then Gallays Silver or Thioflavine S for the tangles. Any comments or suggestions? Any one have any experience with these stains for this purpose? The gliotic plaques are more confounding. I have seen a slide where HE was used and the eosin stained the plaques bright pink-red. Is this typical? Otherwise, I was thinking about PTAH. Anyone have a favourite method for this purpose? I have about 400 slides to go through so an IHC method is out, being to expensive. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histomorphometry
Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 82, Issue 35
1. We do not count folds. 2. Slide turnaround - but that is usually when we are short staffed - our pathology group does very little complaining - I know we are lucky! 3. First run of slide goes up at 730 and every 45 minutes after until all is out - we shoot for 1030. 4. Ordered by noon comes out the same day; after noon goes on the overnight run. Nancy Schmitt HT, MLT (ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext.142 Date: Mon, 27 Sep 2010 09:57:00 -0400 From: Clare Thornton cthorn...@dahlchase.com Subject: [Histonet] QA Questions To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: c9d78ffc9d668b4cbea4405f84697504f801d1d...@iris.dahlchase.net Content-Type: text/plain; charset=us-ascii I would love to hear some other labs' responses to these questions. For clarification, we are a private clinical lab, and we average about 50,000 cases/year. 1. What percentage of slides to you have that have greater than 1 fold? 2. On average what do your pathologists complain about as far as quality? 3. What is your Turn Around Time? What time do the pathologists start getting slides and what time do they have all of their work for the day? 4. What is your IHC turn around time? If the pathologists order IHC tests before what time do they have same day turn around time? Clare J.Thornton, HTL (ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -- Message: 4 Date: Mon, 27 Sep 2010 11:02:01 -0400 From: Weems, Joyce jwe...@sjha.org Subject: [Histonet] RE: QA Questions To: Clare Thornton cthorn...@dahlchase.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e16403986f3...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii 1. We don't count folds. 2. Quality of special stains and immunos. 3. First slides are ready by 8:00 and all out by 11:00. 4. IHCs ordered by noon are out same day. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tyramide
Hello world...So trying to amplify my signal using tyramide. = ; I bought a kit from Invitrogen to use with fluorescence and the fluoresce= nce didn't work at all! The PHD that is helping with this said that h= e thought we should try it using DAB instead of the fluor. I called t= he Invitrogen technical help...WOW!!! I have never had my own questio= n repeated back to me so many times, that guy was a moron on IHC, rude, and= should be fired!!! So I come to ya'll!! Any idea where I can b= uy tyramide to be used with a DAB developer? My primary antibody is a= lready conjugated with HRP, I just want to amplify with with the tyramide a= nd stain with DAB/hematoxylin. Any help would be awesome!!! Sarah Goebel, B.A., HT (ASCP) Histotechnician = iX= Biotech USA Inc. 8201 East Riverside= Dr. Bldg 4 Suite 100 Austin, Texas= nbsp; 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tyramide
I've had good luck with the biotinyl tyramide system from Perkin-Elmer Here is what I do using an unlabeled primary antibody 1) Block endogenous peroxide since you use peroxidase chemistry for this to work 2) Block avidin 3) Block biotin 4) Block with TNB buffer (the kit's equivalent of blocking serum) 5) Add primary antibody 6) Add biotinylated secondary antibody 7) Add SA-HRP (comes with kit) 8) Add biotinyl tyramide reagent. The HRP will deposit biotinylated tyramide near your antigen. 9) Add SA-HRP 10) Add DAB It works well. It also works if you replace step 9 with SA-fluorophore and don't use DAB at all. If your primary antibody is already labeled with HRP, you could do try this: 1) Block endogenous peroxide since you use peroxidase chemistry for this to work 2) Block avidin 3) Block biotin 4) Block with TNB buffer (the kit's equivalent of blocking serum) 5) Add primary HRP conjugated antibody 6) Add biotinyl tyramide reagent 7) Add SA-HRP 8) Add DAB I've never tried it, but I'd expect it won't amplify as well as my method. However, I see no reason why it shouldn't work. Good luck, Adam On Tue, Sep 28, 2010 at 11:13 AM, sgoe...@xbiotech.com wrote: Hello world...So trying to amplify my signal using tyramide. ; I bought a kit from Invitrogen to use with fluorescence and the fluoresce nce didn't work at all! The PHD that is helping with this said that h e thought we should try it using DAB instead of the fluor. I called t he Invitrogen technical help...WOW!!! I have never had my own questio n repeated back to me so many times, that guy was a moron on IHC, rude, and should be fired!!! So I come to ya'll!! Any idea where I can b uy tyramide to be used with a DAB developer? My primary antibody is a lready conjugated with HRP, I just want to amplify with with the tyramide a nd stain with DAB/hematoxylin. Any help would be awesome!!! Sarah Goebel, B.A., HT (ASCP) Histotechnician iX Biotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas nbsp; 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoTech Position In Tuscaloosa
FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma HE Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfa...@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] histomorphometry
Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
Hi Louise, If your lab is like other labs and are a bit strapped for money but need basic point counting, you may also be able do this using ImageJ. ImageJ is a free piece of software developed at and provided by NIH. It has grid and point overlays and counting tools. The learning curve isn't very steep, and it's free! http://rsbweb.nih.gov/ij/ There is also a pc version. Good luck Yak-Nam Wang On 9/28/10 1:47 PM, Wagner, Richard [BUL/LAK] richard.wag...@buehler.com wrote: Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histomorphometry
We use MetaMorph software. It comes with a digital camera for taking the pictures. You can get the software only if you have already the micrioscope and the camera. You can easily measure anything you want, but it is costly. Michelle USC School of Medicine LA CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vectastain elite ABC kit- multiple dips ok?
Hi all, I have three 24-well plates with three brain sections in each well. For IHC, do I have to prepare three different/fresh ABC dips for each 24-well screen plate or can I incubate (30min) each successive screen plate into the already used dip of ABC? Do I have to leave it in longer to allow adequate binding? I know you can do this with DAB but you have a visual indicator that you've left it in long enough in that case. Any experience with this? Thanks, -Teresa -- __ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
