Re: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
thanks Amos which one did you used ? you have catalog number ? On Fri, Oct 1, 2010 at 9:44 PM, Amos Brooks amosbro...@gmail.com wrote: Hi, I am currently working on HMGB1. It is a weird one! If you go by the data sheets you will get great nuclear staining on all the nuclei. This is not what you are after if you have necrotic tissue. What you want to see is the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I am cutting the dilution to 1: 50 and 1:100 since we were just starting to see blushes of cytoplasmic staining and no nuclear label at higher dilutions. Usually you get staining that fades out at higher dilutions. This one was really odd. Good luck, Amos Message: 13 Date: Thu, 30 Sep 2010 04:55:02 +0200 From: Fabrice GANKAM gan...@googlemail.com Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies To: histonet@lists.utsouthwestern.edu Message-ID: c4a46c631cd14a86aad3ce3abb29a...@pcdegankam Content-Type: text/plain; charset=us-ascii Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 3-6 month temp position in Gaithesburg Maryland
`HT or HTL ASCP-tech will perform duties in a veterinary histology lab at Medimmune in Gaithersburg Md. Tech should be able to perform full-tissue necropsies on rodents, subsequent trimming, perform manual and automated tissue processing, decals, embedding in OCT and paraffin, microtomy and cryotomy, manual and automated HE staining and coverslipping. Should be proficient in manually performed special stains and immunohistochemistry. Should possess excellent troubleshooting skills, be familiar with GLP procedures and documentation. Tech should be clean and organized in the performance of his or her duties. The tech will be an Aerotek employee/contractor for Medimmune, not a Medimmune employee. This would be a straight up temp position, no benefits such as 401K, medical or dental. 20-40 hours per week. Perfect position for a person in between jobs, semi-retired etc. If you bring an exceptional set of skills with you but do not possess one of the skills listed above, we may work around that say you are excellent at necropsy but are not good at specials or IHC. Please submit resumes to Aerotek on your won to get the ball rolling, and then send a resume for the hiring manager Nick Madary at mada...@medimmune.com. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PI for Pathologists
Our pathologists do not like the CAP survey for their performance improvement program in Surgical Pathology. Does anyone know of any other company/organization that might offer something like this for our pathologists? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Direct immunofluoresent question
Hi all - The pathologist that reads our direct IF slides likes to have the sections of tissue circled on the slide so that they are easier to find. We currently use black marker on the back of the slide, but find that many times it smears and are looking for a better solution. What kind of slides do others use, and if you circle your sections what marker/pen/etcher do you use? Any thoughts are greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DIF Transport Media
Is Michel Medium the same as Zeus? Do these need to be refrigerated? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Direct immunofluoresent question
I used to mark on the slide, around the section, with a wax marker. Once dried and the procedure completed, cover and observe. René J. --- On Mon, 10/4/10, ba...@gundluth.org ba...@gundluth.org wrote: From: ba...@gundluth.org ba...@gundluth.org Subject: [Histonet] Direct immunofluoresent question To: histonet@lists.utsouthwestern.edu Date: Monday, October 4, 2010, 1:49 PM Hi all - The pathologist that reads our direct IF slides likes to have the sections of tissue circled on the slide so that they are easier to find. We currently use black marker on the back of the slide, but find that many times it smears and are looking for a better solution. What kind of slides do others use, and if you circle your sections what marker/pen/etcher do you use? Any thoughts are greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC unfixed human brain tissue
I have limited experience working with human brain tissue and would like to stain some human brain sections that I received. They were not fixed, but were flash frozen and sectioned on a cryostat, and mounted onto slides. Could someone give me a brief run down of their standard methodological staining procedure with unfixed human brain sections? Much appreciated Neil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: PI for Pathologists
From: Laurie Colbert laurie.colb...@huntingtonhospital.com Subject: [Histonet] PI for Pathologists Laurie Colbert at Huntington Hospital in Pasadena CA asks: Our pathologists do not like the CAP survey for their performance improvement program in Surgical Pathology. Does anyone know of any other company/organization that might offer something like this for our pathologists? Do your pathologists have any input into this decision? I think there are some local programs in California. You're probably referring to the PIP - the Performance Improvement Program in Surgical Pathology of the College of American Pathologists. I've subscribed since 1993 and have never thought very much of it. The CAP need to avail themselves of the new virtual slide technology (such as Aperio's) - this will become more attractive with the new DICOM image standards for pathology - and re-do the PIP program. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Marking inks
The following might be of use Marking surgical margins are often required for determining the adequacy of excision of lesions. Sometimes up to 6 margins (medial, lateral, superior, inferior, superial and deep margins) are required to be individually marked. The properties of a good marker include: 1. It is quick to dry. 2. It is easy to apply. 3. It has a long shelf life. 4. It is cheap. 5. It is non-toxic. 6. It does not spread beyond the edge of the marked area. 7. It is preserved during processing (no contamination of solutions). 8. It is visible macroscopically and microscopically. 9. Sectioning is not affected. 10. It is radiolucent. 11. There are multiple colours available. India Ink, a colloidal suspension of particulate carbon in aqueous gum has been used for many years, but is messy, slow to dry and spreads readily over the tissue surface. 10% Silver Nitrate in methanol has been suggested but it also spreads over the surface and is macroscopically invisible. 5% alcian blue in 80% ethanol is an excellent tissue marker. It is easy to apply, quick to dry, has a long shelf life and is preserved during processing. Birch et al (1990) have suggested that specimens can be dunked into 1% aqueous alcian blue. This method is quick, cheap and the coating is radiolucent. Harris (1990) has suggested the use of Tippex fluid. This is a solution of titanium dioxide and polyacrylate in trichloethane. It has a long shelf life, is easy to use, quick drying, non-toxic and the tissue processor is not affected. The marker does however have a tendency to lift and is radiodense. Hunter-Craig et al (1991) rolled blotted dry specimens in starch powder. The surface coating of starch is visible on light microscopy but strikingly apparent if crossed polarisers are used. Artist's pigments in acetone (a 50% solution) were used by Patterson and Davies (1988). The pigments are quick drying, have a good range of different colours that are visible both macroscopically and microscopically (especially using polarised light) and are resistant to processing. They are however, radiodense and have a short storage life. It must be remembered that the dry powders (which contain cobalt, manganese and cadmium) are toxic. The pigment granules are also of similar size and density to microcalcification and there may be confusion on subsequent specimen mammography after tissue slicing. Suggested pigments are as follows: PIGMENT COLOURMACROSCOPIC MICROSCOPIC Cobalt Blue BlueBlue Alizarin CrimsonRed Red ViridianGreen Green Armstrong et al (1990) have described the use of organically coloured gelatines, where 8% solution of dye is prepared in 24% aqueous gelatine. They found it easier to discriminate between colours of particulate dyes (i.e. plant substances), with the gelatine staining with both haematoxylin and eosin giving a pink to purple colour. They found clear demarcation between two adjacent colours and only a modest knowledge of botany was required for the identification of the three plant materials. DYE COLOUR MACROSCOPIC MICROSCOPIC Janus Green BluePurple India Ink Black Black Paprika Red/brown Red-pigmented cellulose Tumeric Yellow Cerebriform starch granules Henna Brown Brown-pigmented cellulose Bismark Brown Brown Brown particles Unfortunately tumeric causes knife scratching. Armstrong et al (1990) J.Clin.Pathol., 43:604-607 Birch et al (1990) J.Clin.Pathol 43:608-609 Harris (1990) J.Clin.Pathol 43:346 Hunter-Craig et al (1991) J.Clin.Pathol. 44:874-875 Patterson Davies (1988) J.Clin.Pathol 41:1013-1016 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, 2 October 2010 3:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Marking inks Richard Cartun asks about marking inks used for surgical pathology specimens. if I just need one color (great majority of specimens) I use india ink bought from a stationer or craft supply store - cheap, easily replaced, and it gets the job done. If I need colors, I prefer the Davidson marking inks, available through some though not all vendors. They offer seven colors, including orange (they have to sell their product in Tennessee and Texas, after all!) and purple. (I have no commercial connection with Davidson inks.) I blot my specimens thoroughly dry, and don't use a fixative. If I did, I'd use white vinegar (5% acetic acid), possibly diluted 1:1 with water. Bouin's fixative and acetone both have obvious
[Histonet] CAP programs
There are now online CAP PIP programs. I have done one on prostate. Also there are online programs for the cytology part. Review the catalogue. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
