RE: [Histonet] carnoys fluid

[IRENA SREBOTNIK KIRBIS]

I would recommend methanol or ethanol for fixation, smears itself can be 
culprit of inconsistent staining
Irena
> To: histonet@lists.utsouthwestern.edu
> From: heather.mcl...@webmail.co.za
> Date: Wed, 24 Nov 2010 19:42:51 +0200
> Subject: [Histonet] carnoys fluid
> 
> Dear Histonetters,
> 
> We do IHC on pap stained cyto smears. The results do not always "behave"
> accordingly on the smears that have been exposed to Carnoys. Does anybody know
> whether Carnoys Fluid affects IHC on cytology smears? I have seen papers that
> suggest improved IHC after Carnoys but maybe Histonetters feel difefrently?
> Thanks 
> Heather
> 
> 
> South Africas premier free email service - www.webmail.co.za 
> 
> Free SMSs everyday! Gratis, Forniet! http://clients.wm.co.za/20030688/
> 
> 
> 
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[Histonet] Crystalization of Schiff's Reagent

[histopathy]

Hello Histonetters,

Has anyone had issues with your Schiff's reagent crystallizing?  I cannot 
remember the cause when it happened one time several years ago.  Any ideas?  
Our refrigerator is the appropriate temperature and the Schiff's reagent is 
brand new...happened to previously ordered bottles, also.

Pam
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Re: [Histonet] Histology Consultant

[Jay Lundgren]

 If you have to ask, you can't afford it.

 Jay A. Lundgren M.S., HTL
(ASCP)
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RE: [Histonet] Cryostat Question Please

[O'Donnell, Bill]

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to "clunk" unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
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[Histonet] Cryostat Question Please

[Paula Lucas]

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula 
Lab Manager
BP Medical Group
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[Histonet] Microtomy SOP

[Breeden, Sara]

I am sorry to say that as I received the SOPs on Microtomy, I printed
and deleted them.  I apologize.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] Help cutting horse tendon.

[Pablo Sanchez-Quinteiro]

Dear listers,

I am trying to cut whole sections of the tendon of the digital flexor 
muscle of the horse. The piece is around 1 mm long and very elastic 
and fibrous. I have tried formalin and Bouin fixation, the latest 
better. With paraffin embedding the fibrous elements of the tendon 
spoil the sections. In freezing microtome the sections seem nice but 
the interfascicullar spaces are too wide at the microscope.


I would very grateful for any hint, suggestion, etc.

Best regards,

Pablo


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[Histonet] evans blue

[Liz Chlipala]

Hello everyone and Happy Thanksgiving

 

Does anyone out there know if I inject evens blue dye in tissue will it
survive 10% NBF fixation and then processing to paraffin block.  Its
supposed to be taken up by cells that are undergoing lysis  Basically I
need to be able to detect cell lysis on paraffin sections.

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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[Histonet] Histology Consultant

[sgoebel]

What is the going rate for a working histology consultant in Texas?  For
instance go in and process, embed, cut, and stain slides when needed?

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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[Histonet] carnoys fluid

Dear Histonetters,

We do IHC on pap stained cyto smears.  The results do not always "behave"
accordingly on the smears that have been exposed to Carnoys. Does anybody know
whether Carnoys Fluid affects IHC on cytology smears?  I have seen papers that
suggest improved IHC after Carnoys but maybe Histonetters feel difefrently?
Thanks 
Heather


South Africas premier free email service - www.webmail.co.za 

Free SMSs  everyday!  Gratis, Forniet! http://clients.wm.co.za/20030688/



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[Histonet] Microtomy SOP

[Breeden, Sara]

Thanks to everyone (seven of you!) that sent me examples of your
microtomy SOP.  I'll pick and choose from all to build my own - and it
will save a lot of time, I'm positive.  I tend to make my SOPs rather
"generic" as far as specific steps, equipment names, etc., at the behest
of my QA manager.  We'd rather not be too specific in case momentary
deviation is required - that's a fine line, let me tell you!

 

Thanks to my Faithful Contributors and have a wonderful Thanksgiving
holiday.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] HT Position

[Hale, Meredith]

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mh...@carisls.com

 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mh...@carisls.com   

 

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[Histonet] HT Position

[Hale, Meredith]

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
ma...@carisls.com 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mh...@carisls.com   

 

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[Histonet] Recall: HT Position

[Hale, Meredith]

Hale, Meredith would like to recall the message, "HT Position ".
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[Histonet] Looking for...

[NYSHisto]

Tim Morkin
Could you please contact me off line?
Thanks

 ---
Luis Chiriboga Ph.D.
Assistant Professor of Pathology
President, New York State Histotechnological Society
NYSHS Website: www.nyhisto.org
NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/


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RE: [Histonet] 70% alcohol

[Molinari, Betsy]

That is how I handle ours as well. Start in 95% and proceed with the normal 
processing protocol.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 23, 2010 3:54 PM
To: histonet@lists.utsouthwestern.edu; sgoe...@mirnarx.com
Subject: Re: [Histonet] 70% alcohol

It is always better to be safe than sorry. You should start in EtOL95% → 100% 
and clear as usual before infiltration.
René J.

--- On Tue, 11/23/10, sgoe...@mirnarx.com  wrote:


From: sgoe...@mirnarx.com 
Subject: [Histonet] 70% alcohol
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, November 23, 2010, 4:48 PM


Hello all!!

I moved jobs, but am still here in 80 degree Austin weather...

Question.  If tissue has been stored in 70% alcohol for up to a year,
does there need to be anything done to it before processing?  I am
assuming that some rehydration might be necessary?  If not can you just
throw it on  the processor on a normal cycle?  Thanks for you help!!



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912



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