[Histonet] hydrometer
CBG Biotech sells hydrometers. There item number is MIS1003.$45. 1-800-941-9484 Jana Ver Beek, HT(ASCP) Spectrum Health-Blodgett Campus Grand Rapids, MI 49506 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hydrometers
E.C. Kraus has hydrometers for $15.00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cheap metal detector
Here is a website for cheap metal detectors for those who need to find staples in specimens for their pathologists. Shirley http://www.woodcraft.com/Product/2003820/9451/Little-Wizard-Metal-Detector.aspx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] embyro embeding
I am cutting whole embryos and neonates. I would prefer to avoid paraffin ( I seem to have issues with varying consistency of tissue between different organs. Ie some are very friable while others cut perfectly all in the same block) and cut frozen on a sliding microtome. However I need to keep the pieces from separating and keep them in the proper orientation. I was going to use gelatin but the pieces fall out even when I vacuum embed and fix the gelatin. I have just joined the list so I don't know all the passed posts. I did see some about HistoGel. Would this allow me to embed with histogel and cut frozen on a sliding microtome and retain a complete section that I could then mount without losing pieces? Also If I use Histogel first would this improve the issues I have with paraffin. Lastly has anyone cut paraffin sections on a sliding microtome. How do you set it up? Blade angle , Perpendicular to block or angled? Thanks Alex ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD20 for IHC on mouse FFPE tissue
Hello, Maybe I'm remembering wrong since I deleted the original message of Bretts looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so anti-murine CD20 that can be made to work on mouse FFPE tissues certainly exist. Have used them from BD (flow reagent), Santa Cruz and others. In Biocompare website, can see them plus new rabbit monoclonal anti-murine CD20 for paraffin, reactive to murine tissues. There are a few CD20+ murine B-cells that have no B220, and vice-versa so I would'nt look for CD45R if I wanted to find CD20+ cells. Would look for CD20+ cells. Maybe I'm remembering original post incorrectly. Ray Ray Koelling PhenoPath Labs Seattle, WA - Original Message - From: Amos Brooks amosbro...@gmail.com To: brett connolly brett_conno...@merck.com, histonet@lists.utsouthwestern.edu Sent: Thursday, November 25, 2010 3:31:27 AM Subject: [Histonet] CD20 for IHC on mouse FFPE tissue Hi Brett, CD20 is not going to work for mouse tissue. You need CD45R (clone B220). This will selectively label B cells just like CD20 in humans. I get this from BD Biosciences (Now Thermo ... again) Catalog # 347460. It is raised in Rats and works great as long as you have a good rat secondary. The one from Jackson is good. Happy Thanksgiving, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Wow Bryan, That's pretty slick. Obviously weighing it would do the trick I hadn't thought of that. I'll have to remember that the next time I mindlessly try to shake the excess liquid off my hydrometer and have it break in my hands. Gosh I hated that! That is definitely a great and probably more precise way of doing it. Amos On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote: Specific gravity is mass/volume. In this context that is grams/millilitre. It can easily be measured without a hydrometer. 1. Obtain a 10 ml beaker and weigh it to 2 decimal places. 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the beaker. 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places. 4. Subtract the weight of the beaker from the weight of the beaker and alcohol, giving the weight of the alcohol 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal places. Bryan Llewellyn - Original Message - From: Amos Brooks amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Sent: Thursday, November 25, 2010 3:14 AM Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Hi Jim, Hydrometers can get really expensive. I searched around for one with a good price and stumbled on this one from Cole Parmer (now Thermo like everyone else in the world): Thermo Scientific ERTCO® Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, Plain Form ... CAT# EW-08285-00 I picked it up for $29.50, but that was with my University discount. I'm not sure what regular price is or what discounts you might be able to get. It sure beat the heck out of some of these $200 ones out there. This one has both ETOH percentages as well as ETOH proofs. It works well for us. Happy Thanksgiving, Amos Message: 4 Date: Tue, 23 Nov 2010 13:02:24 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a55510fe3...@mmc- mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD20 for IHC on mouse FFPE tissue
Thanks Brett, Looks like I got a couple lines crossed there. I had been told that there wasn't any CD20 in mice. I have been using B220 for BCell detection. After you pointed out the error I looked it up at Biocompare and clearly there are some CD20 markers for mice. http://tinyurl.com/38wyh42 I was also apparently wrong about BD being a Thermo company. Thanks Peter. They do distribute it, but don't own it. My mistake ... again :-) With those goofs I'll shut up now and blame the turkey Enjoy your weekend guys, Amos On Friday 26 November 2010 04:08:06 pm koelli...@comcast.net wrote: Hello, Maybe I'm remembering wrong since I deleted the original message of Bretts looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so anti-murine CD20 that can be made to work on mouse FFPE tissues certainly exist. Have used them from BD (flow reagent), Santa Cruz and others. In Biocompare website, can see them plus new rabbit monoclonal anti-murine CD20 for paraffin, reactive to murine tissues. There are a few CD20+ murine B-cells that have no B220, and vice-versa so I would'nt look for CD45R if I wanted to find CD20+ cells. Would look for CD20+ cells. Maybe I'm remembering original post incorrectly. Ray Ray Koelling PhenoPath Labs Seattle, WA - Original Message - From: Amos Brooks amosbro...@gmail.com To: brett connolly brett_conno...@merck.com, histonet@lists.utsouthwestern.edu Sent: Thursday, November 25, 2010 3:31:27 AM Subject: [Histonet] CD20 for IHC on mouse FFPE tissue Hi Brett, CD20 is not going to work for mouse tissue. You need CD45R (clone B220). This will selectively label B cells just like CD20 in humans. I get this from BD Biosciences (Now Thermo ... again) Catalog # 347460. It is raised in Rats and works great as long as you have a good rat secondary. The one from Jackson is good. Happy Thanksgiving, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GU pathology laboratory looking for histotechs/ cytotechs in Columbus, Ohio
Looking for experienced histology technicians and cytotechnologists in Columbus, Ohio for new GU pathology laboratory. Excellent compensation with great benefits. Experience with IHC, FISH, and GU pathology a plus. Send resume or CV to care...@aksm.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
