[Histonet] Mohs HE QC
What is the easiest way to be compliant with the CLIA guideline of a daily HE positive and negative control slide in a Mohs lab? Would a slide with a known positive and a known negative precut and stored in the freezer, then one run with the first case be acceptable? Anyone performing Mohs, please let me know how you are handling this. Thanks, Kari CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] used equipment
Hello All, I'm an amateur microscopist, mostly interested in botanical specimens. I recently acquired an American Optical A820 microtome. It appears to be in good working condition but is missing the blade. I am considering purchasing a used blade or a used disposable blade holder from Ebay or a lab surplus website. I realise the condition of this material may not be optimal and unfortunately the sellers generally do not know the history of these items. My question is - should I be worried about safety? If there is a chance this material came from pathology labs, do I need worry about any potential prions/viruses etc? Thanks in advance, Matthew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mohs HE QC
Hi Kari, I worked doing Mohs for over 10 years and the way that we handled the issue of a quality control slide is that typically you can find some positive and negative areas within the first patient sample. We would take the very first sample and section for the control slide and run it down to ensure that the stainer was set-up correctly and that the solutions were still viable. If you have any other questions please feel free to contact me. Thanks!!! Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breal, Kari Sent: Wednesday, December 01, 2010 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mohs HE QC What is the easiest way to be compliant with the CLIA guideline of a daily HE positive and negative control slide in a Mohs lab? Would a slide with a known positive and a known negative precut and stored in the freezer, then one run with the first case be acceptable? Anyone performing Mohs, please let me know how you are handling this. Thanks, Kari CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sentinel nodes
Good morning all, I've been having a strange artifact on sentinel nodes, in only one lab of the two where I perform frozen sectioning services. This artifact does not show up any any other tissue type either. The node is usually almost totally covered with a bubbling, like the tissue is not laying flat. There can be intermittent unaffected areas. These aren't round bubbles, more rectangular and almost orderly in their pattern. I can do nodes in the other lab and they are fine. A colleague here was wondering if the tissue came in saline or some other liquid, which it hasn't. Any ideas? Laurie King HT (ASCP __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] sentinel nodes
Crystals maybe? How cold is the cryostat? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of King, Laurie Sent: Wednesday, December 01, 2010 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sentinel nodes Good morning all, I've been having a strange artifact on sentinel nodes, in only one lab of the two where I perform frozen sectioning services. This artifact does not show up any any other tissue type either. The node is usually almost totally covered with a bubbling, like the tissue is not laying flat. There can be intermittent unaffected areas. These aren't round bubbles, more rectangular and almost orderly in their pattern. I can do nodes in the other lab and they are fine. A colleague here was wondering if the tissue came in saline or some other liquid, which it hasn't. Any ideas? Laurie King HT (ASCP __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] The Milestone PATHOS
Has anyone used or is currently using The Milestone PATHOS for tissue processing? What are your results, pros and cons? Would this be good for breast biopsies? Thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] c-erbB2
Yes. What's up with that? Mark Cindy Bulmer cjbul...@sbcglobal.net wrote: Hello Histoland, Has anyone seen any B-9 ducts staining positive with the c-erbB2 Oncoprotein (Rabbit, conc.) # A0485 from Dako? Thank you, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special Stain Automation
We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. PYLORI STAIN
Hi, I was wondering if anyone has used the Poly Scientific Rapid Staining Method for H. Pylori in Gastric Biopsies staining kit. Is it easy to use? Is the stain crisp and easy to read? Any feedback would be appreciated. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Special Stain Automation
The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Special Stain Automation
I recommend the Artisan from DAKO. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 10:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special Stain Automation
Barbara, We are using the Dako Artisan Link for our special stains and love it. We are getting set to interface the Link to our LIS (SoftPath). This will give us the capability to use the barcodes printed on our slides to bring up the pathologist order directly on the Artisan. We also do a fair number of GMS stains on Non-gyn Thin Prep slides for respiratory specimens looking for Pneumocystis. I would recommend getting a demo from the Dako rep. Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question from one of our researchers
Can someone give me ideas to pass along to one of our researchers? Of course adhesion molecule antibodies are the first thought, but not for heterogeneous cell populations. So I was wondering maybe an antibody cocktail? Are there any lectins that might show this? Thanks! I am wondering what kind of cell membrane marker is recommended to stain tissue section for imaging (going to process the image with 3D Imaris software). Cells are mouse tissue section in paraffin and very heterogeneous. To see cell-cell boundary clearly. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question from one of our researchers
While not a cell membrane marker, you can often use differential interference contrast (DIC) microscopy. The microscope I use has a special setting that lets you add DIC without any additional staining. In the mouse bone marrow paraffin sections, it lets you see most edges of most cells. Adam On Wed, Dec 1, 2010 at 11:19 AM, Johnson, Teri t...@stowers.org wrote: Can someone give me ideas to pass along to one of our researchers? Of course adhesion molecule antibodies are the first thought, but not for heterogeneous cell populations. So I was wondering maybe an antibody cocktail? Are there any lectins that might show this? Thanks! I am wondering what kind of cell membrane marker is recommended to stain tissue section for imaging (going to process the image with 3D Imaris software). Cells are mouse tissue section in paraffin and very heterogeneous. To see cell-cell boundary clearly. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Small biopsy specimens
We have been using eosin to stain our small biopsies prior to tissue processing so that they are easier to see when embedding. Recently one of our PA's started using Hematoxylin instead of the eosin, because that is what they used where she trained. Upon further investigation the manufacturers of both of our tissue processors discourage the use of eosin because it is very hard on the working parts of the machines and requires more PM's to prevent damage. So we are considering switching to using Heme instead. Do any of you out there use Heme on your biopsy specimens? Do your embedders find it easier to see these tiny tissues when embedding? Any pros or cons greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Small biopsy specimens
We've always only used hematoxylin where ever I've worked. I haven't embedded in eons so can't tell you how visible those specks of tissue are. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, December 01, 2010 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Small biopsy specimens We have been using eosin to stain our small biopsies prior to tissue processing so that they are easier to see when embedding. Recently one of our PA's started using Hematoxylin instead of the eosin, because that is what they used where she trained. Upon further investigation the manufacturers of both of our tissue processors discourage the use of eosin because it is very hard on the working parts of the machines and requires more PM's to prevent damage. So we are considering switching to using Heme instead. Do any of you out there use Heme on your biopsy specimens? Do your embedders find it easier to see these tiny tissues when embedding? Any pros or cons greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Special Stain Automation
Just wondering how many people hate the Artisan's plastic well things that you have to clip on the slides?? Nails, Felton flna...@texaschildrens.org 12/1/2010 11:06 AM The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special stain Automation
Does anyone have experience with the Gemini ES from Thermo Shandon? - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] positive slides
Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the slides. Thank You Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Special Stain Automation
Don't have a problem with them at all Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, December 01, 2010 1:30 PM To: 'Barbara Richmond'; histonet@lists.utsouthwestern.edu; Felton Nails Subject: [Histonet] RE: Special Stain Automation Just wondering how many people hate the Artisan's plastic well things that you have to clip on the slides?? Nails, Felton flna...@texaschildrens.org 12/1/2010 11:06 AM The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] positive slides
We use VWR Superfrost Slides Cat # 48311-703 and they are great, no lifting sections. On 12/1/10 1:57 PM, zodia...@comcast.net zodia...@comcast.net wrote: Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the slides. Thank You Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shurwave Microwave
Histonetters , does anyone have a protocol for large polyps 0.5 cm and larger using the TBS Shurwave Microwave Processor. Thanks Histojoe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Position
Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mh...@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com mailto:mh...@carisls.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part time postition for histology lab assistant
We have a part time with benefits histology lab assistant positio= n open in Hillsboro, OR. Go to [1]www.Tuality.org for more information if interested. W= e are looking for someone to gross with the pathologists and handle the acc= essioning and closing of our department. The hours are M-F 12-5 pm. Cathy Crumpton HT(ASCP), Histology Lead Tu= ality Community Hospital Hillsboro, OR 97123 (503)681-1292 = /FONT References 1. 3Dhttp://www.Tuality.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] High temp/paraffin oven
Hello to all, Any idea why my middle paraffin oven would be out of range, but the othet two on opposite sides are not? Histopathy Sent on the Sprint® Now Network from my BlackBerry®___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 7200 VLC
Hello Histonetters! Hope everyone had a nice Thanksgiving. I am using Technovit 7200 VLC kit to embed undecalcified 5-8mm, (varies per sample), thick sections of vertebral samples. If anyone has experience with infiltration times and length of time under yellow and blue light and would not mind sharing that knowledge, I would greatly appreciate it! Thank you, Payal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hellerstrom Hellman anyone?
Hi, I just did a Hellerstrom Hellman alcoholic silver stain for pancreatic D- cells. I followed the method described by Bancroft Stevens. When the slides were placed into the reducing solution they instantly precipitated into a toasty mess, the solution turned milky brown and the slides (even around the sections) picked up a lot of silver precipitate. If anyone has done this of could offer suggestions about it, I'd really like to trouble shoot the stain a bit. A bit of background about the stain that I thought I should add. According to the published method I used a 10% silver solution in ETOH with 100ul of 1M nitric acid. The pH of the solution was 5.0. The reducing solution was alcoholic formalin with 5% pyrogallic acid. I thought the silver solution seemed really concentrated (It hardly even disolved). Incubating such a solution overnight at 37 deg C (as indicated in the method) seemed a bit much. Has anyone heard of modifications of this stain? There is no sense reinventing the wheel, but I certainly don't want to repeat it knowing that it will innevitably do the same thing again unless there is something glaringly obvious that I did wrong. Thanks, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question from one of our researchers
Hi, Teri, How about this? It also gives a brief blurb in the description about commonly used alternatives, if this isn't your thing. I've never tried it, so I can't vouch for it myself, but Invitrogen does make some pretty darn good stuff... http://probes.invitrogen.com/media/pis/mp10045.pdf Sincerely, Nicole Collette Lawrence Livermore National Lab On 12/1/10 9:19 AM, Johnson, Teri t...@stowers.org wrote: Can someone give me ideas to pass along to one of our researchers? Of course adhesion molecule antibodies are the first thought, but not for heterogeneous cell populations. So I was wondering maybe an antibody cocktail? Are there any lectins that might show this? Thanks! I am wondering what kind of cell membrane marker is recommended to stain tissue section for imaging (going to process the image with 3D Imaris software). Cells are mouse tissue section in paraffin and very heterogeneous. To see cell-cell boundary clearly. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] High temp/paraffin oven
Not as much room/air flow for vents to dissipate extra heat? Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of histopa...@hotmail.com Sent: Wed 12/1/2010 4:19 PM To: Histonet Subject: [Histonet] High temp/paraffin oven Hello to all, Any idea why my middle paraffin oven would be out of range, but the othet two on opposite sides are not? Histopathy Sent on the Sprint® Now Network from my BlackBerry® ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hellerstrom Hellman anyone?
Did you acid clean your glassware before hand? Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amos Brooks Sent: Wed 12/1/2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hellerstrom Hellman anyone? Hi, I just did a Hellerstrom Hellman alcoholic silver stain for pancreatic D- cells. I followed the method described by Bancroft Stevens. When the slides were placed into the reducing solution they instantly precipitated into a toasty mess, the solution turned milky brown and the slides (even around the sections) picked up a lot of silver precipitate. If anyone has done this of could offer suggestions about it, I'd really like to trouble shoot the stain a bit. A bit of background about the stain that I thought I should add. According to the published method I used a 10% silver solution in ETOH with 100ul of 1M nitric acid. The pH of the solution was 5.0. The reducing solution was alcoholic formalin with 5% pyrogallic acid. I thought the silver solution seemed really concentrated (It hardly even disolved). Incubating such a solution overnight at 37 deg C (as indicated in the method) seemed a bit much. Has anyone heard of modifications of this stain? There is no sense reinventing the wheel, but I certainly don't want to repeat it knowing that it will innevitably do the same thing again unless there is something glaringly obvious that I did wrong. Thanks, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
