[Histonet] 53BP1 and gamma-H2AX antibodies
Good morning Histonet, Does anyone know of 53BP1 and gamma-H2AX antibodies that work in FFPE rat tissue (lung, liver, spleen, brain). Would appreciate any suggestions anyone might have, Best wishes, Michele Dr Michele Ellender Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Health Protection Agency, Chilton, Didcot, Oxon. OX11 ORQ UK Phone: +44 (0)1235 822697 Fax: +44 (0)1235 833891 E-mail michele.ellen...@hpa.org.uk mailto:michele.ellen...@hpa.org.uk - ** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question
In power outages ( bumps) does the Sakura cryostat resent micron settings? ( factory setting? ) I figure that this would be the fastest way to get an answer. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Jobs in NY
Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on several open positions with a fast paced and expanding speciality laboratory in Westchester County. This company is provides transportation directly from the city to their lab. They are a speciality pathology company offering anatomic, molecular, digital, and local testing services. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus (1st or 3rd shift or Per Diem) * Experienced Histotech - NYS licensed histotech (2nd shift or Per Diem) * Cyto Prep Technician * Mycology Technician - Per Diem * PCR Tech - 1st shift They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for someone with muscle biopsy experience in NY
Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must * Muscle/Nerve Technician - Must have muscle biopsy experience They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: 53BP1 and gamma-H2AX antibodies
Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody #2577 from Cell Signaling works very well in FFPE(dilution 1:100 in immunofluorescence). Don't know good anti 53BP1. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Ellender Sent: Thursday, February 03, 2011 5:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 53BP1 and gamma-H2AX antibodies Good morning Histonet, Does anyone know of 53BP1 and gamma-H2AX antibodies that work in FFPE rat tissue (lung, liver, spleen, brain). Would appreciate any suggestions anyone might have, Best wishes, Michele Dr Michele Ellender Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Health Protection Agency, Chilton, Didcot, Oxon. OX11 ORQ UK Phone: +44 (0)1235 822697 Fax: +44 (0)1235 833891 E-mail michele.ellen...@hpa.org.uk mailto:michele.ellen...@hpa.org.uk - ** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] about histonet mailing list
could you pls delete my e-mail adress from mailing list thanks ozge -- *Mutlu Günler :) Özge KARADENİZ* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CLIA classes?
Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 Fixation Requirement Responses/Thanks
Your information and suggestions helped me formulate a plan, so thank you to those who took the time and responded to my inquiry yesterday. Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] CLIA classes?
I work at at derm office and this would be great to know about if there was one. Please please please include me in the list of responses. Thanks! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sands, Jenn Sent: Thursday, February 03, 2011 9:03 AM To: indytreeg...@sbcglobal.net; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA classes? I would be interested in this information as well. Thank you. Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of indytreeg...@sbcglobal.net Sent: Thursday, February 03, 2011 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA classes? Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Trace Gas companies
Thanks to all who gave the info. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Katelin Lester histol...@medsurgpath.com 02/02/2011 08:11 PM To kim.dona...@bhcpns.org cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Trace Gas companies We just did ours this week! We get them from Environmental Monitoring Technology. http://www.emt-online.com/ Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 Hi Histonetters Can you all share with me where you get and send in your trace gas analysis badges? Thanks a bunch! :-D Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] slide labeler advice
Hi Liz, We use Leica's IPC and IPS equipment and have found a simple solution to using them with our LIS. If you connect a computer (CPU) only, to each IPC or IPS you want to interface, you can use these units like any other printer that your LIS lists. We routinely print slides and cassettes automatically when a case is accessioned or on demand from any of our workstations. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, February 02, 2011 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labeler advice Hello again We are in the market for a slide labeler. We currently have the SlideMate from Thermo Scientific. We have had it for about 2 years now and we are just not happy with the quality and consistency of the printing. We are a contract research lab and not a clinical lab. Entering data for each slide is not an option. The programming that comes with the Slide Mate is nice, except that no one has really spent the time to train us on it, we have figured it out a bit. We would want a labeler that we could create formats for different sample types, so all we would have to do is put the animal ID in and the printer would print all of the slides needed. Would like barcoding capabilities just incase we move to that in the future. Any advice or suggestions are appreciated. Vendors welcome also. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formaldehyde monitor
Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Consult accessioning question
When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new Date-of-service? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Consult accessioning question
Richard, We use the date of accessioning as the DOS and we wouldn't accession it until we have received the specimen/materials. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/3/2011 10:43 AM, Richard Cartun wrote: When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new Date-of-service? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formaldehyde monitor
Lab Safety Supply has the option to order the badges for formaldehyde and Xylene and when you order, you pre-pay for the analysis. Formalin is $476.00 for 5 monitors. Xylene is $259.00 for 4 monitors. They email you a very nice report for your file, and it only takes about a week to 10 days. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formaldehyde monitor
Call Advanced Chemical Sensors Inc @ 561-338-3116 I think they are like $100 each. Last I looked anyway. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Bob Nienhuis bob.nienh...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 02/03/2011 12:29 PM Please respond to bob.nienh...@gmail.com To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Consult accessioning question
Same for us. You need both DOS from office as well as date received. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Thursday, February 03, 2011 1:06 PM To: rcar...@harthosp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Consult accessioning question In our LIS system we put in specimen date or DOS and we also have a Received date. My understanding, for insurance/billing purposes, you would need to include the original DOS, but you also need to be able to document when you actually received the specimen. Just my 2 cents!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 1:43 PM To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new Date-of-service? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Off Topic: researchers very funny
yes our lab received it last week! And yes we've been posting it to Facebook as well (as someone else on Histonet commented!) If the lady in the video never gets her PhD, she may want to consider the entertainment industry! --On Tuesday, February 01, 2011 10:29 PM -0500 Kimberly Tuttle ktut...@umm.edu wrote: If this is a repost I apologize :) http://www.youtube.com/watch?v=Fl4L4M8m4d0feature=player_embedded This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Consult accessioning question
For Medicare, my understanding would be to use the DOS if less than 30 days, and to use the date pulled from archives if more than 30 days. At least that is the regs for additional tests. I think it would be the same for consult as well. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 13:43 To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new Date-of-service? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TTF-1/background staining
How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing
Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Formaldehyde monitor
We use Advanced Chemical Sensors. Acsbadge.com is website. I also used to use Surgipath. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave processing
Dorothy: You should follow for MW processing the same procedure you follow for any traditional tissue processing. What you have to document is that when you started to use the MW processing there was a documented QC to compare the results between the method you used and the new with the MW processor. Having to sign a QC after each run is absolutely unnecessary if the initial QC was done. René J. --- On Thu, 2/3/11, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] Microwave processing To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Thursday, February 3, 2011, 3:38 PM Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temporary histology position in San Diego Ca.
GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications: Associate's or B.S. degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. American Society of Clinical Pathologist (ASCP) certification as a HT or HTL is required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. The employee must have 2-5 years of experience in the Histology lab performing animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions: 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs Microtomy on rotary microtome, cryostat, and be able to use a sliding microtome. 4. Prepares dyes and solutions in order to perform routine, special, and complex procedures. 5. Have experience in histochemical stains and enzymatic histochemical stains. Be able to trouble shoot and correct problems with histochemical stains. Have experience in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning Instrumentation. Interested candidates please send a copy of your CV with a summary of research experience, and three references. Job Code: JW10-013 Contact:E-mail: j...@gnf.orgmailto:j...@gnf.org James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel858-332-4647 Fax 858-812-1915mailto:858-812-1915jwat...@gnf.org jwat...@gnf.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TTF-1/background staining
Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Her2 Fixation Requirement
Does anyone have issues with inking the specimen after it has been placed in formalin? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, February 02, 2011 12:59 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document the cold ischmic time - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. The pathologists document all this time in the report. Best! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, February 02, 2011 12:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Karnovsky and Roots stain
I am looking into a project involving motor end plate staining. Literature that I've found continually references Karnovsky and Roots from the 60s. However the papers are not supplying all the details. Does anyone do AchE staining by this method on fresh frozen, unfixed tissue sections? If so, can I get a more detailed protocol (fixation steps, washes, etc)? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TTF-1/background staining
Hi Paula I do not believe that RTU Abs really exist. No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab. I have used many RTU Abs which I have had to dilute to get optimal staining with Cam 5.2 a good example. On the Bond I was using it at 1:25. try titreing your Ab and see if that will reduce/eliminate your background. regards Tony Debra Siena dsi...@statlab.com 4/02/2011 8:02 am Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TTF-1/background staining
Hi Paula, You mentioned the staining in a liver specimen using the cell marque antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is known to stain liver cells and liver cancer. Your pathologist might be interested in knowing that. Here is a reference you can give him: http://www.nordiqc.org/Run-23-B5/Assessment/Assessment-TTF-1.htm It also lists another clone that doesn't cross react with liver. Mark On Thu, Feb 3, 2011 at 12:38 PM, Paula Lucas plu...@biopath.org wrote: How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
