Re: [Histonet] Section position on slides
Hi Tanya, It's always best to train people to place the section in the middle of the slide. It depends if one has to put multiple sections on a slide. Learning how to do proper placement will help them when they have to cut controls for IHC that are often times placed on the top of the slide. Sections placed too close to the very bottom or top sometimes don't get stained or coverslipped. Too close to the edges and you can suffer wrap around or overlap, causing the section to be difficult for the pathologist to read. Boy howdy! They will let you know if they don't like the placement. Therefore, train for centering the section as best as possible. I hope this helps, Paula :-) On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem t...@hotmail.com wrote: I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. “x” mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Na K ATpase immunohistochemistry on rat FFPE tissue
Does any body have experienced on Anti Na K ATpase immunohistochemistry on FFPE rat kidney tissue? I am finding difficult to have any supportive lit regarding this, most of them support to frozen sectioning. Thanks a lot Amita ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Wage question for Wisconsin
At least you have a union!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 15, 2011 6:48 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Wage question for Wisconsin The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later this week. The techs at the UW hospital have fortunately signed their contract that is good until the reopener in 1013 and we are not strictly state employees. However, the way things are looking, it is not out of the question that he may also try to void our contracts. Our pay MAY go up, but we will no longer have much, if anything, for benefits. Don't count on sending your kids to the UW colleges if this goes through. Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this is HUGE for millions of Wisconsinites. GGR! Claire Were there any other Wisconsin techs at the rally today? Even the Police and Firefighters unions were down there! From: histonet-boun...@lists.utsouthwestern.edu on behalf of Michael Hillmer Sent: Tue 2/15/2011 5:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wage question for Wisconsin We are dermatology clinic in Northeast Wisconsin and we are trying to do gather accurate wage data. Can anybody offer wages for an HT, HtL and lab assistants? Any help would be greatly appreciated. Thank you- Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Section position on slides
I learned from an old school HT and studied using the Carson book. In this book there are several pages on where to put sections. If you don't have the Frieda Carson book (or the bible as I refer to it), get it. A new edition just came out about a year ago. If you're an ASCP member I think the price is reduced... For the most part, just put everything as close to the middle of the clear glass part of the slide as possible. This also does well for IHC automation. I think most automated machines you can adjust the drop range too if you want to put the sections on the bottom or top? Good Luck! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Tuesday, February 15, 2011 11:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. x mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: saliva for glycogen hydrolysis
I agree, gum sounds better not to mention the taste CG -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 15, 2011 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Mon 2/14/2011 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: saliva for glycogen hydrolysis Here's a truly hi-tech suggestion for doing PAS-diastase stains by the spit method (which by the way is still how it's done by the small pathology services I work on): You can produce copious quantities of saliva by the simple expedient of chewing on a rubber band for a couple of minutes. This used to be the technique used to obtain saliva samples for determination of ABH substance secretor status in the blood bank. (I belong to that elite 20% of donors who are non-secretors, and Lewis-a positive to prove it.) Bob Richmond Samurai pathologist and occasional sialogogue Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Wage question for Wisconsin
Michael, ASCP has a wage/salary survey that they distribute based upon region and certification. Some have said that this is biased as not everyone does participate. I tend to agree with the survey, but also recognize that dermatopathology is a 'specialty' type lab. I worked in Dermpath at NYU back in the 80's to early 90's and our salaries were not identical to the clinical labs as we were a part of the medical school and not the hospital. Yes the hospital was 1199 at that time, I cannot say if they are still. My recommendation is to go with what you can find from the survey, but also network with other dermpath or other specialty labs in your area that you may know or who you can connect with through others - either on the listserver or off - to possibly get a better sounding of salaries. HR at your hospital might be also able to help if you have someone there that you can work with and trust to obtain some of the data you're looking for and understands what your specific needs are within the lab. I would include your pathologists in this as much as possible as they will be needed to support your request(s) as they are essentially your clients. Vikki On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer mhill...@dermwisconsin.com wrote: We are dermatology clinic in Northeast Wisconsin and we are trying to do gather accurate wage data. Can anybody offer wages for an HT, HtL and lab assistants? Any help would be greatly appreciated. Thank you- Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 Fax: (920)686-9674 Cell: (920)860-6360 The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Correction, Correction on cutoff date for GSH meeting
Correction: There is a correction on the cutoff date for the hotel reservations. Cutoff date for discounted room rate is February 23rd, Not March 1st. There are several other events going on that weekend and the hotel is filling up fast. Please make your reservations and register soon so you will not miss out on the great room rate and a great meeting. Shirley Powell Hi Georgia, Alabama, ALL histotechs, The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is METAMORPHOSIS: Transforming Histotechs. The complete program can be downloaded from our website at this link: www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathco double-blade handle
Am looking for this item to replace one that was older than I am. I need the handle (perhaps Pathco #D2877) for the blades (Pathco #D2878-2C). The catch is that I want the SOLID BRASS handle - not the aluminum. I've googled all over the place - can anyone help me find these handles? Muchas gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pathco double-blade handle
I've found two brass handles on Ebay in the last few years. I don't know if you're aware of this but the exact same blades that fit these handles can be purchased at Home Depot in the carpet section. $10 for 50 of them. Jim ___ James E. Staruk HT(ASCP) http://www.masshistology.com www.masshistology.com www.nehorselabs.com From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 16, 2011 11:00 AM To: Histonet Subject: [Histonet] Pathco double-blade handle Am looking for this item to replace one that was older than I am. I need the handle (perhaps Pathco #D2877) for the blades (Pathco #D2878-2C). The catch is that I want the SOLID BRASS handle - not the aluminum. I've googled all over the place - can anyone help me find these handles? Muchas gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1204 / Virus Database: 1435/3447 - Release Date: 02/16/11 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Exam Prep
Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Exam Prep
I just used the Freida Carson Histotechnology A Self-Instructional Test. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of caymanfl...@gmail.com Sent: Wednesday, February 16, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Section position on slides
As a pathologist I have some preferences on how certain tissues are oriented on a slide and work with my histotechs so they can learn my prefs. I also like my slides in certain orientation in the slide tray. EG, for orientation of skin, I like the the dermis at the top when I am looking at the slide. Some other tissues can be so oriented, some cannot. We often put multiple levels on one slide and I would prefer them to be all oriented similarly and in a straight line. The most annoying thing I see beyond coverslipping the wrong side, is the label covering a part of the specimen. I don't care how close to the edge a section is as long as it is all on the slide and covered by the cover-slip or mounting medium. I have no idea is these preferences are weird. My point is the answer to you 1st question may depend on your particular pathologists' preferences. BIll Blank At 10:09 PM -0700 2/15/11, Tanya Ewing-Finchem wrote: 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. ³x² mm from the edge of the glass slide)? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Section position on slides
I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the top. The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. x mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Section position on slides
Since automation is becoming more and more a part of all Histology labs the demands of placement of the tissue on the slides varies for different instruments. Stainers, coverslippers and now with slide scanning as well. So I do not believe that there is a silver bullet answer. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 16, 2011 12:21 PM To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Section position on slides I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the top. The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. x mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Apologize for Spam
Someone has hacked into my email and it is sending nonsense emails to everyone in my address book. I have changed my password to prevent this from happening again. Again, I am truly sorry. Roxanne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Section position on slides
Automated coverslipping instruments are defined by the manufacturer, but modifications on some systems can be done. Most clinical labs who utilize automation use 24X50mm - as this coverslip will cover most of the slide. Tissue placement is pretty much determined by laboratory needs. Sheehan Hrapchak had a short segment about tissue placement, but it was in regards to the tissue orientation for microscopic assessment requirements. There is nothing in granite about tissue placement that I know of. Some tools that I worked with were using the Cytospin large rectangular slides to show residents/students/collegues where the tissue placement needed to be. Once they were able to get the tissue section in there we moved on. While to most of us who work in Histo sort of take putting sections on a slide for granted (yes we did work hard to get there) getting the mechanics of it takes patience, skill and practice. Keep it simple and build up from there. Vikki On Wed, Feb 16, 2011 at 12:27 PM, Helen Fedor hfe...@jhmi.edu wrote: Since automation is becoming more and more a part of all Histology labs the demands of placement of the tissue on the slides varies for different instruments. Stainers, coverslippers and now with slide scanning as well. So I do not believe that there is a silver bullet answer. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 16, 2011 12:21 PM To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Section position on slides I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the top. The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. x mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Information Please
Wanting to get information on anyone that has already implemented Epic and offered the Epic ambulatory piece to there clients. Are you also offering this technology with interface to competitive laboratories like, Quest, Lab Corp, etc? __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Rep.
Can a Ventana Representative for the Southern Virginia region, please give me a call at the number below. Please only a Ventana rep. I do not have authorization to deal with another company at this time. Thank you. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Exam Prep
I also used Freida Carson, Histotechnology A Self-Instructional Test, 3rd Edition. As well as, the Freida Carson, Companion Study Flash Cards that goes along with the 3rd Edition text book. Study the objectives and your golden. Rochan Moir-Dial, HTL(ASCP) Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 Phone: (303) 682-3949 Fax (303) 682-9060 roc...@premierlab.com www.premierlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Wednesday, February 16, 2011 9:24 AM To: caymanfl...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Exam Prep I just used the Freida Carson Histotechnology A Self-Instructional Test. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of caymanfl...@gmail.com Sent: Wednesday, February 16, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Colorado Sate Histo Meeting, April 29-30, 2011
Hi, All are welcome. It is a great program this year. Attached is the program for the 2011 CSH meeting, which will be held April 29th 30th at the Embassy Suites hotel in Loveland, CO. Online registration and credit card payment is available on the CSH website at http://www.coloradohisto.org/2011/meeting.htm Ciao Matt Lunetta HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NY lab permit
Hello all, Anyone out there have experience in applying for a NY state lab permit? How long did it take? How difficult was it? Could anyone recommend a lab consultant on this task? Thanks in advance, -- *Marian L. Powers, BSOM, HT(ASCP) * * * * * * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] neutrophil antibody on rat FFP section
Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. Could anybody recommend a good antibody? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CellSoft vs. Sodium Hydroxide
Greetings one all: Has anyone tried CellSoft from CellPath in the UK? I received a sample today and after reviewing the MSDS that came with it, I'm skeptical about the claim in their literature that it reduces the hazard risk for the user. I've got 10% sodium hydroxide on hand for softening nails so if anyone has already done a product comparison, I'd love to hear from you. Thanks. Dorothy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: saliva for glycogen hydrolysis
From: Ingles Claire cing...@uwhealth.org Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been! Claire Back in my early days in the lab, when I was in the US Navy (wooden ships and Folin-Wu glucose), I was involved in a project that required us to collect fairly large (10-15 mL) saliva samples from Navy recruits who were part of a research study. We used a wax gum, which I believe was manufactured by Wrigley's gum company. The sticks were the same size and shape as gum (back then gum looked like a microscope slide, not all the shapes that are currently available) and they were individually wrapped like gum. They tasted like paraffin, but they induced copious salivation, and served our purpose well. I don't know if this product is still available. Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Novolink polymer kit
Thanks Michelle and all histonetters for your suggestions. After your feed back, i am finalizing the Novolink Polymer-HRP detection kit for my work. I am going to use this kit for single labelling of the antibody. i will let u know about my result as it comes. Amita ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
