[Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Hi Diane, We have been happy with the Leica cryostats over the years. We have an 1800 that is about 15 years old, an 1850 that is about 5 years old with the UV light for decontamination. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Esophageal Brushings
I would make smears and a cell block(if enough specimen) and charge 88104 and 88305. Pap on the smear, HE on the cell block. Jan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, March 29, 2011 4:19 PM To: Cristi stephenson; Histo Net Subject: RE: [Histonet] Esophageal Brushings I would charge an 88104 also. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, March 29, 2011 4:10 PM To: Histo Net Subject: [Histonet] Esophageal Brushings Hello Histoland, I was wondering if anyone can help me with a CPT code question? I run a small GI lab. We have recently received esophageal brushings that were to rule out cancer, not Candida. The pathologist indicated these should be stained with the Papinicalaou stain. I was thinking per the archives, that this would be charged as an 88313 (non-organism stain) as we charge an 88312 for the fungal stain. However, the other facilities around my area say that it would be charged as an 88104. Can onyone offer me some guidance? Thank you all, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana's Basal Cell Cocktail
To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Peripheral smear
We also transport smears from the clinical lab to our Pathologist and transport them back. The Hematology techs put the Pathologist's comments into our computer system for review by the clinicians. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, March 29, 2011 4:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral smear Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laura Miller is Out of the Office.
I will be out of the office starting 03/25/2011 and will not return until 04/04/2011. I am on vacation until Monday, April 4th. Please contact David Archambault if you need immediate assistance. Thanks!!! __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breast Processing
Breast Processing: Looking to set up a breast protocol on our Sakura VIP processor. Looking for times/duration of specific solutions and types of solutions used (ie. PEN-FIX). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whiteplains, NY: Histology Tech, IHC Techs, Leads Manager Positions
Allied Search Partners has been retained for the following searches. We have openings in *Histology/IHC, for techs, leads, and managers. * - Please email a copy of updated resume tomeli...@alliedsearchpartners.com aly...@alliedsearchpartners.com for a full job description. - Please send availability for a phone screen with one of our recruiters. - Please indicate your salary expectations. - Referral bonuses are available for all positions so please pass this along to anyone you deem fit. We have the following positions available: *HISTOLOGY/IHC:* ** *IHC Manager* Whiteplains, NY area Monday-Friday, Day Shift, Full Time *NY license required *IHC Tech III (Lead)* Whiteplains, NY area Schedule, TBD *NY License Required *IHC Tech II * White Plains, NY area 7am-3:30pm Tuesday-Saturday 10am-6:30pm Monday-Friday *NY License Required *Histotech I* Whiteplains, NY area M-F 5am-1:30pm *NY license required **Ask us about our other histotech managerial positions in WI, NC, CA, LA, FL. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with “Remove.” * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana's Basal Cell Cocktail
Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Peripheral smear
Gary Martin (where?) asks: Our pathologist[s] review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. I take these VERY seriously. First, how NOT to do it: 1. Pathologist scribbles a tiny note in a space the size of a postage stamp, which then gets posted to the chart, or maybe doesn't. 2. Pathologist scribbles a note, which a technologist then types into the system (pathologist doesn't have access to the system). 3. Pathologist dictates a note, which is typed by a transcriptionist unfamiliar with the vocabulary, then put in the chart where the attending physician will never find it. Peripheral smear reviews are requests for consultation and should be treated as such. This consultation is billable (CPT 85060). There is a higher CPT code for more extensive consultation (80502) but I've never talked to anyone who'd heard of it. The pathologist should review the history to the extent necessary, and record this review in the note. (The patient doesn't live whose relevant history I can't summarize in one sprawling, badly written sentence.) The consult should include the basic data from the blood count that comes with the smear, since this information probably won't be simultaneously available to the physician reading the consult. The blood smear review should be focused in view of the information available. The pathologist should recommend such basic tests (reticulocyte count, vitamin B12, ferritin, direct antiglobulin test) as haven't been done. I prefer to type these consults myself, but most pathologists dictate them. A template would be worthwhile. The point here is - the pathologist is a consultant, not a technician, and should not regard themself as a technician nor allow themself to be treated as a technician. These reviews can be the best bargain in laboratory medicine, given that the pathologist would make better money flippin' burgers at McDonald's. I get enough feedback about them to know they're worthwhile. The opinions expressed are my own, and have not been vetted by anybody with an MBA. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Peripheral smear
To add to my earlier diatribe: I do not want a slide dripping with immersion oil to mess up my microscope. I expect you to mount the smear in resin. Many histotechnologists are uncomfortable doing this, so I often coverslip the slide myself. I'll use immersion oil on the coverslipped slide if I need to - depending on the quality of my high-dry lens and on my elderly eyes. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rita Humphrey
Hello Histoland! Does anyone know how to get in touch with Rita Humphrey, previously with Childrens Hospital, Birmingham, AL? I lost touch with her after she left Childrens and would like to re-connect. Thanks in advance, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 88, Issue 39
Thank to everyone who responded to my request for information concerning peripheral smear review. It seems that most respondents are doing this the way we presently do. I have to say that I agree totally with Dr. Richmond's method. I will be presenting the idea that these slides are consults not just a tehc issue. Many thanks Gary (in California) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, March 30, 2011 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Peripheral smear (wanda.sm...@hcahealthcare.com) 2. Laura Miller is Out of the Office. (laura.mil...@leica-microsystems.com) 3. Breast Processing (daystar0...@aol.com) 4. Whiteplains, NY: Histology Tech, IHC Techs, Leads Manager Positions (Alyssa Peterson) 5. RE: Ventana's Basal Cell Cocktail (Sebree Linda A) 6. Re: Peripheral smear (Robert Richmond) -- Message: 1 Date: Wed, 30 Mar 2011 09:45:29 -0500 From: wanda.sm...@hcahealthcare.com Subject: [Histonet] RE: Peripheral smear To: gmar...@marshallmedical.org, histonet@lists.utsouthwestern.edu Message-ID: 9e2d36ce2d7cba4a94d9b22e8328a3ba13a275f...@nadcwpmsgcms03.hca.corpad.ne t Content-Type: text/plain; charset=us-ascii We also transport smears from the clinical lab to our Pathologist and transport them back. The Hematology techs put the Pathologist's comments into our computer system for review by the clinicians. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, March 29, 2011 4:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral smear Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 30 Mar 2011 10:00:49 -0500 From: laura.mil...@leica-microsystems.com Subject: [Histonet] Laura Miller is Out of the Office. To: histonet@lists.utsouthwestern.edu Message-ID: OF69753508.1B5BFDDC-ON86257863.00527906-86257863.00527906@leica-microsy stems.com Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 03/25/2011 and will not return until 04/04/2011. I am on vacation until Monday, April 4th. Please contact David Archambault if you need immediate assistance. Thanks!!! __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ -- Message: 3 Date: Wed, 30 Mar 2011 11:56:07 -0400 (EDT) From: daystar0...@aol.com Subject: [Histonet] Breast Processing To: histonet@lists.utsouthwestern.edu Message-ID: 8cdbd0a1d7f79fe-dd4-5...@webmail-m104.sysops.aol.com Content-Type: text/plain; charset=us-ascii Breast Processing: Looking to set up a breast protocol on our Sakura VIP processor. Looking for times/duration of specific solutions and types of solutions used (ie. PEN-FIX).
Re: [Histonet] Peripheral smear
We accession the peripheral smear into our Clinical Microscopy module of the computer program. We stain the slide, coverslip it and give it to the pathologist with a hematology CBC print out. The pathologist interprets the slide and dictates the report. The transcriptionist types the report (much like any surgical report). The pathologist reviews the completed transcription and either makes corrections or electronically finalizes it. It then is available to all personnel to view in the computer system. We charge an 85060 for the professional fee. I am not aware of a technical fee we can charge. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Martin, Gary gmar...@marshallmedical.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/29/2011 04:39 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Peripheral smear Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Florida Law Question - again
Hi Histonetters! I posted this the other day, but I guess it nested itself in the April Fools email. So, I thought I would post it again under its own heading! Can anyone tell me (maybe even provide a reg) as to whether or not a lab aide can run the Histos 5 microwave tissue processor? It's semi-automatic, which involves the person (lab aide, tech etc) moving a carousel of cassettes from one beaker to another. Does anyone know if this is considered to be waived, moderate or high complexity testing? Any help is greatly appreciated! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Florida Law Question - again
Hi Michelle, This issue has been discussed at the clinical board meeting and it has been said that moving a carousel from one beaker to another is considered part of the specimen processing steps, making it part of a licensed task. The histology scope of practice includes as a licensed task specimen processing, see Rule 64B3-10.005 (11). This is the same issue with loading and unloading IHC instruments. The purpose of the specialty of histology is to process cellular and tissue components through methods of fixation, dehydration, embedding, microtomy, frozen sectioning, staining, and other related procedures and techniques employed in the preparation of smears, slides, and tissues. This specialty also encompasses methods for antigen detection and other molecular hybridization testing methods where the purpose is analysis and/or quantification of cellular and tissue components for interpretation by a qualified physician. Technicians licensed in histology are limited to the performance of specimen processing, embedding, cutting, routine and special histologic staining, frozen sectioning and mounting of preparations under the general supervision of a director, supervisor, or technologist. Hope this helps! Jerry Santiago, MSEd, HTL(ASCP)QIHC Florida State College Jacksonville Assistant Professor/HT Program Director 4501 Capper Road Jacksonville, FL 32218 Tel: 904-766-6528 Fax: 904-766-5573 e-mail:jsant...@fscj.edu From: histot...@imagesbyhopper.com histot...@imagesbyhopper.com To: histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 2:39:48 PM Subject: [Histonet] Florida Law Question - again Hi Histonetters! I posted this the other day, but I guess it nested itself in the April Fools email. So, I thought I would post it again under its own heading! Can anyone tell me (maybe even provide a reg) as to whether or not a lab aide can run the Histos 5 microwave tissue processor? It's semi-automatic, which involves the person (lab aide, tech etc) moving a carousel of cassettes from one beaker to another. Does anyone know if this is considered to be waived, moderate or high complexity testing? Any help is greatly appreciated! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] to microwave or not to microwave
Dr. Sendelbach, We had a microwave processor in the past, and switched back to a routine shortened biopsy run for several reasons. It is possible that the technology since then has improved to where you would not experience issues. However, microwave processing usually carries additional regulations (such as CAP for non-vented systems) and some machines require a lot of tech time to maintain. Most of the reagents suggested/sold for use with the microwave processors are alcohol or acetone based, which seemed to decrease the quality of our staining and sectioning. We have a shortened routine biopsy run on a vacuum processor that seems to do the job in the same amount of time with less additional maintenance and without the alcohol-fixed appearance in the tissue. Thank you, Ann Lowder, HT(ASCP) Message: 2 Date: Mon, 28 Mar 2011 12:44:02 -0500 From: Karla Sendelbach karla.sendelb...@thedacare.org Subject: [Histonet] to microwave or not to microwave To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: dc2665b881ef7a4b99275c654e4eec070fd32...@exmb4.ad.local Content-Type: text/plain; charset=us-ascii We are evaluating rapid tissue processing instruments. I would appreciate any comments about microwave tissue process and rapid non microwave tissue process. Peloris II, STP 420ES and Milestone Pathos come to mind. Also, is there anyone who switched from microwave processing to rapid non microwave tissue processing? Thank you, Dr. Sendelbach Appleton Medical Center Appleton Wisconsin 920.738.6294 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Weigert's Iron Hematoxylin
Hi Histonetters, I have a quick question regarding Weigert's Iron Hematoxylin and Gill's 3 Hematoxylin. One of the guys here thinks that we can interchange these 2 stains. I am attempting to perform a Masson Trichrome stain and it calls for Weigert's Iron Hematoxylin. This person in my lab says to just use Gill's Hematoxylin in place of the Weigert's stain. Is this right? Thanks for your help Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Weigert's Iron Hematoxylin
Iron hematoxylin is more resistant to decolorization than aluminum hematoxylin. Since there are so many acid steps that follow the hematoxylin, it is wise to use the Weigert's so you do not have to worry about decolorizing the other tissue elements too far. However, if you optimize your stain to the use of the Gill's and the pathologists agree that staining intensity is optimal then it should be fine. I wouldn't do it without optimizing first. So overall, the two are not exactly interchangeable since some of the subsequent staining steps may have to be altered. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blundon, Kimberly Sent: Wednesday, March 30, 2011 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Weigert's Iron Hematoxylin Hi Histonetters, I have a quick question regarding Weigert's Iron Hematoxylin and Gill's 3 Hematoxylin. One of the guys here thinks that we can interchange these 2 stains. I am attempting to perform a Masson Trichrome stain and it calls for Weigert's Iron Hematoxylin. This person in my lab says to just use Gill's Hematoxylin in place of the Weigert's stain. Is this right? Thanks for your help Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana's Basal Cell Cocktail
Sharon, I do a CK34BE12 P63 cocktail for basal cells charge for only one stain. That being said, you can absolutely charge for 2 as P63 in nuclear the CK34BE12 is cytoplasmic, so the pathologist can discern one from the other. I am considering setting it up as a double charge in the future if our volume for it continues to climb as it has been lately. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, March 30, 2011 11:10 AM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana's Basal Cell Cocktail Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Weigert's Iron Hematoxylin
Hi Kimberly, Imho because of the color, Weigert's Iron Hematoxylin is dark brown. Gill's 3 is blue. anilin blue is also blue. imho it would be better stain with Weigert's Iron Hematoxylin. On Wed, Mar 30, 2011 at 10:55 PM, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote: Hi Histonetters, I have a quick question regarding Weigert's Iron Hematoxylin and Gill's 3 Hematoxylin. One of the guys here thinks that we can interchange these 2 stains. I am attempting to perform a Masson Trichrome stain and it calls for Weigert's Iron Hematoxylin. This person in my lab says to just use Gill's Hematoxylin in place of the Weigert's stain. Is this right? Thanks for your help Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in regards to review of peripheral smears
In our lab, the hematology dept has a form that they present to the pathologist with a manual differential( if only an automated one was done originally) and CBC with abnormal results , and a list of check boxes indicating whether they agree with technologist interp or if a written consult is needed. At THAT time the case is then accessioned into the anatomic side, a report generated with pathologist's interp, recommendation etc and appropriate charges are made. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for a good anti-mouse CD3 for formalin fixed sections
Hi, Could anyone suggest a good anti-mouse CD3? I am looking at staining aortic root frozen sections fixed with formalin. Thanks! Delphine Delphine Eberlé PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121, USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Hi Diane, We just purchased a cryostat from Sakura, we love it. Great features. Date: Wed, 30 Mar 2011 06:38:41 -0700 From: dch...@yahoo.com To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) CC: Hi Diane, We have been happy with the Leica cryostats over the years. We have an 1800 that is about 15 years old, an 1850 that is about 5 years old with the UV light for decontamination. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
