[Histonet] Ordering Chemicals
Dear Histonet Members, I am trying to build a neuroscience laboratory from scratch in Turkey. As you can imagine, there are many different things, I have to deal with. I have some questions about ordering basic chemicals for histology. Since there are many different qualities of the same product (for example; 21 different NaOH in Sigma catalog), I am not sure which one is better for histology and immunohistochemistry. Could it be possible to write me the company name and the catalog number of the chemicals, that I have listed below, so I can order correct ones? Any other suggestions and advices, as well as donations would greatly be appreciated. Thank you very much in advance. Sincerely, Salim Yalcin Inan, PhD Department of Pharmacology Meram Faculty of Medicine University of Selcuk 42080 Meram, Konya, Turkey Phone: +90 507 206 9662 (cellular) Phone: +90 332 223 6624 (work) E-mail: salim.i...@gmail.com FOR PBS SOLUTION - Sodium Chloride - Potassium Chloride - Sodium Phosphate (monobasic) - Potassium Phosphate (monobasic) FOR SUCROSE SOLUTION - Sucrose - Sodium Azide FOR PFA SOLUTION - Paraformaldehyte - Sodium Hydroxide - Hydrogen Chloride FOR CRESYL VIOLET STAINING - Cresyl Violet - Glacial Acedic Acid - Absolute Ethanol - Xylene or Histoclear - Mounting media (Permount) - Ready to use microscope slides and cover slips ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Incubator sale
In an attempt to make room for new equipment, Sage colleges is selling a 4.5 cu ft Fisher Isotemp Forced-Air Incubator model 650F. Anyone interested please contact me by email or at the number below. Anna Schleifstein SCA Bio Lab Coordinator Sage Colleges Albany NY 12208 518- 292-1748 sch...@sage.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] National Average Salary
So apparently I'm underpaid =) Good Luck convincing employers of this! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, March 31, 2011 9:35 AM To: Lin Bustamante Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] National Average Salary
Oh I think you will find that 98% of us are underpaid. :) -Original Message- From: sgoe...@mirnarx.com [mailto:sgoe...@mirnarx.com] Sent: Thursday, March 31, 2011 10:48 AM To: Shirley A. Powell; lbustama...@cvm.tamu.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary So apparently I'm underpaid =) Good Luck convincing employers of this! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, March 31, 2011 9:35 AM To: Lin Bustamante Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peripheral smear review
Gary Martin, I'd be very interested in knowing what response you get to your presentation. I'm certainly available by e-mail if anybody wants to know more. Remember when you copy my note to include the comment about coverslipping which I posted later. I'd add further: The pathologist's task in reviewing the slides is to correlate the patient's history, the microscopic appearance of the blood smear, and the blood count data, in order to produce a consultation report useful to the attending physician in caring for the patient. Bob Richmond Samurai Pathologist Knoxville TN rsrichm...@gmail.com ** From: Martin, Gary gmar...@marshallmedical.org Subject: [Histonet] RE: Histonet Digest, Vol 88, Issue 39 To: histonet@lists.utsouthwestern.edu Thanks to everyone who responded to my request for information concerning peripheral smear review. It seems that most respondents are doing this the way we presently do. I have to say that I agree totally with Dr. Richmond's method. I will be presenting the idea that these slides are consults not just a tech issue. Many thanks Gary (in California) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] National Average Salary
Thank you very much for the information. Lin Bustamante. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 Shirley A. Powell 03/31/11 9:35 AM Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I
I will be out of the office starting 03/31/2011 and will not return until 04/01/2011. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Acriflavine staining of alginate for mucopolysaccharides
Hi All, I am new to histology. I came across the protocol below for staining GAGs (I plan to use that for alginate) in an online book 'Theory and practice of histological techniques', By John D. Bancroft, Marilyn Gamble. The protocol is by Hollander dated 1963. Would like to know if anyone has used this protocol before? Any tips/specific details might be helpful. The 20% HCl - does it mean 20% of 37.3% concentrated hydrochloric acid? Any details about acriflavine to be used would be helpful. Thank you. Regards, Sujata The protocol is as below: Protocol Acriflavine-DMAB method for sulfatide (Hollander 1963) Fixation and sections Post-fixed cryostat sections; formal calcium-fixed frozen sections Preparation of reagents 1.Acriflavine stock solution Acriflavine 100mg Distilled water at 80 deg C 20ml Store in dark at 4 deg C 2.Acriflavine working solution 0.1M citrate-HCl buffer pH 2.5 99ml Stock acriflavine solution 1ml 3.DMAB solution p-dimethylaminobenzaldehyde0.6g 20% HCl 30ml Isopropanol 70ml Method 1. Mount sections onto slides 2. Stain for 6 minutes in acriflavine solution 3. Differentiate for 1 minute in two changes of 70% isopropanol 4. Treat with DMAB reagent for 30-45 seconds 5. Rinse in distilled water for 2-3 minutes 6. Counterstain nuclei in Mayer’s or Carazzi’s hematoxylin 7. Blue in tap water, rinse in distilled water and mount sections in glycerin jelly. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GFP labelled cells after decalcification...are they still labelled?
Hi All A first time user of Histonet here -with a quick question for research histologists... Has anyone ever attempted to locate GFP expressing cells in bone-marrow sections after decalcification and paraffin embedding? (I guess my question really is: Does the decalcification process destroy or weaken the GFP signal) Any advice would be greatly appreciated! Regards James Dr James Hart BVSc MS Comparative Orthopaedics, Cornell University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat repair
Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GFP labelled cells after decalcification...are they still labelled?
Hi James, I do this all the time in mouse bones. I've found that EGFP is very robust, and it can survive fixation with PFA and Zn buffered formalin, as well as decalcification with EDTA or formic acid, followed by paraffin embedding. However, the endogenous fluorescence is highly quenched by this process and barely detectable over background if at all. In order to detect it post embedding, I use the chicken anti-GFP polyclonal from Abcam, and a fluorophore conjugated donkey anti-chicken IgY with very good results. If you want endogenous fluorescence, you can fix the bones in 4% PFA and decalcify using EDTA, and use frozen sections. I hope this helps, Adam On Thu, Mar 31, 2011 at 12:42 PM, James Hart jameshar...@gmail.com wrote: Hi All A first time user of Histonet here -with a quick question for research histologists... Has anyone ever attempted to locate GFP expressing cells in bone-marrow sections after decalcification and paraffin embedding? (I guess my question really is: Does the decalcification process destroy or weaken the GFP signal) Any advice would be greatly appreciated! Regards James Dr James Hart BVSc MS Comparative Orthopaedics, Cornell University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paula Wilder
Paula Wilder if you get this message please call Dianna Fetter at 443-552-2968. I know you have retired but I am trying to reach you. I would like to speak with you. Happy retirement Dianna P.S I'm writing this from Caula's computer...Hence where the message is from ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acriflavine staining of alginate for mucopolysaccharides
Greetings Sujata: On 3/31/2011 1:39 PM, Sujata Sovani wrote: Hi All, I am new to histology. I came across the protocol below for staining GAGs (I plan to use that for alginate) in an online book 'Theory and practice of histological techniques', By John D. Bancroft, Marilyn Gamble. A good book. The protocol is by Hollander dated 1963. A very old, but not necessarily bad, method. Why do you want to use this particular method? There are simpler ways to do this. Alcohol fixation might retain GAGs better ... how about staining with 1% Alcian Blue in 3% acetic acid? Would like to know if anyone has used this protocol before? Any tips/specific details might be helpful. The 20% HCl - does it mean 20% of 37.3% concentrated hydrochloric acid? Yes. Any details about acriflavine to be used would be helpful. Can't help you there. I'll bet DMAB is very toxic. Geoff Thank you. Regards, Sujata The protocol is as below: Protocol Acriflavine-DMAB method for sulfatide (Hollander 1963) Fixation and sections Post-fixed cryostat sections; formal calcium-fixed frozen sections Preparation of reagents 1.Acriflavine stock solution Acriflavine 100mg Distilled water at 80 deg C 20ml Store in dark at 4 deg C 2.Acriflavine working solution 0.1M citrate-HCl buffer pH 2.5 99ml Stock acriflavine solution 1ml 3.DMAB solution p-dimethylaminobenzaldehyde0.6g 20% HCl 30ml Isopropanol 70ml Method 1. Mount sections onto slides 2. Stain for 6 minutes in acriflavine solution 3. Differentiate for 1 minute in two changes of 70% isopropanol 4. Treat with DMAB reagent for 30-45 seconds 5. Rinse in distilled water for 2-3 minutes 6. Counterstain nuclei in Mayer’s or Carazzi’s hematoxylin 7. Blue in tap water, rinse in distilled water and mount sections in glycerin jelly. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat repair
Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CoPath Users in CT (Eastern)
Hi, I am looking for a hospital/lab which uses the CoPath system for daily operations within the Eastern parts of CT. Kimberly Brown Histology Lab Manager Lawrence and Memorial Hospital Phone # 860-442-0711 ext. 4383 This message (and any included attachments) is from Lawrence Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat repair
We use them too- Illinois office. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bouchal, Rena L Sent: Thursday, March 31, 2011 1:38 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair We are using Tech One Biomedical... service reps in Pghfor almost all of our routine instrument service really good and half the price of the vendors! Their number is 866-497-3033 or www.techoneweb.com Please note that my email address as of Jan 3, 2011 is bouch...@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, March 31, 2011 2:15 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] another money saving question
Is there any way to extend the life of PAP pens? Add a little xylene to the tip or soak it in something? I feel like they run out so fast. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Kappa/Lambda ISH on Ventana
Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Poll on B5
Hello all. I was asked by our director to try to come up with a list of places that do still use and do not still use B5. We are working hard on trying to get to be a mercury free facility and other than the B5 we use for bone marrows we are mercury free. So, if you all could be so kind as to send me a response that would be much appreciated. I would like to know what you currently use, if you used B5 in the past or if you are currently using B5 now, and hospital name. I would absolutely appreciate any and all comments and help you all can provide. Thank you! Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic, Rochester MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biopsy Run
Hi again Kim, From your previous emails, it seems to be something your tissue processors cannot handle. Review your equipment and consumables. Below, is (almost) everything I think about when I hear Some tissues are staining/processing irregularly: Formalin? Formalin-substitute? Recycled? Who makes your formalin? Some inconsistencies in vendor bulk formalin cubes have been noted. Processors? You said you have two. What types or brands? Dip-and-dunk (open) or infiltration chamber (closed)? If closed-type, when did your folks last clean or replace the tubing? Retort gasket? Cassette-type? Can your cassettes Float in the processor? Biopsy sponges used? http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-April/004782.html? Alcohol? Check with a Hydrometer? Who grades your alcohol? Recycled? Xylene or xylene-substitute? Recycled? Infiltrating Paraffin? Temperature? Confirm with a thermometer? Embedding - Temperature? Cassettes in holding tank for too long? Mictotomy - Soak your blocks in water/ice? Drying - How long in incubator? Temperature? As for your question about biopsy processing times, refer to Histotechnology A Self-Instructional Text, any edition, by Freida L. Carson, ASCP Press. In the 2nd edition pp 32-34 (note there is a troubleshooting portion too), Protocol #3 lists basically the processing run that I do overnight (and I think you do to), but I start in formalin. Everything is listed as 15 minutes, but it will also depend on what types of tissues are defined as Biopsies, and you should extend the times as needed. I use 30 minutes, with some longer as listed. Pressure and vacuum is up to you, but I only use it throughout the paraffin steps. 10% alcohol 95% alcohol 95% alcohol 100% alcohol 100% alcohol (45 minutes) Xylene Xylene (45 minutes) Paraffin Paraffin Paraffin (45 minutes) Investigate these options and respond if needed. Hope this helps, Hugh-Hawaii Date: Mon, 28 Mar 2011 12:32:32 -0800 From: kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Run Hello out there!!! Just a couple of quick questions...I wrote before with a artifact problem and I thank everyone for their input. Bad thing is, I am still having issues. Would my fellow Histo Techs please share with me the times you use on your Biopsy Run's as well maybe give me some advise on biopsy cassettes. I have used 2 different kind and just cant help but wonder if air getting caught in the cassette may be one of my problems. Thanks in advance for your help...I sure need it. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Poll on B5
We still use it but are in the process of evaluating alternatives in order to phase it out. So far it looks like B-plus is winning. Lisa Children's Memorial Hospital- Chicago -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Thursday, March 31, 2011 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Poll on B5 Hello all. I was asked by our director to try to come up with a list of places that do still use and do not still use B5. We are working hard on trying to get to be a mercury free facility and other than the B5 we use for bone marrows we are mercury free. So, if you all could be so kind as to send me a response that would be much appreciated. I would like to know what you currently use, if you used B5 in the past or if you are currently using B5 now, and hospital name. I would absolutely appreciate any and all comments and help you all can provide. Thank you! Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic, Rochester MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Kappa/Lambda ISH on Ventana
You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Common requisition for Histology and Cytology
Hi everyone, Does anyone use a common requisition for histology and cytology (and maybe flow too)? If yes would you be willing to share a copy of your requsition. If not do you know of any location who does? Thanks for your time, Andrea Beharry Technical Specialist Pathology Department William Osler Health System 2100 Bovaird Drive East Brampton, ON Canada L6R 3J7 * andrea.beha...@williamoslerhs.ca NEW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
