RE: [Histonet] How to keep paraffin sections from sticking toblade/blade holder.
Check the retraction on the back of the mictrotome. Switch to the other setting -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 21, 2011 3:26 PM To: Histonet list serv.; BretClough Subject: Re: [Histonet] How to keep paraffin sections from sticking toblade/blade holder. 1- change cutting angle 2- keep the blade holder clean (without paraffin) 3- make sure the blade is not blunt René J. --- On Thu, 4/21/11, Clough, Bret clo...@medicine.tamhsc.edu wrote: From: Clough, Bret clo...@medicine.tamhsc.edu Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. To: Histonet list serv. histonet@lists.utsouthwestern.edu Date: Thursday, April 21, 2011, 3:07 PM While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Input needed
You've picked a great profession for lots of vacancies. Of course, at any given moment, in any given location, there might not be an opening, but if you are willing/able to move, there are lots of openings. And many places are willing to pay moving expenses, or sign on bonuses. Just ask, if they don't advertise that they might be willing to pay some extra. ASCP LabMedicine just published their wage and vacancy survey results in March and April 2011 issues. (Thank you to everyone who is an ASCP member, whose dues help pay for this!) Vacancy: http://labmed.ascpjournals.org/content/42/4/199.full.pdf+html Wages: http://labmed.ascpjournals.org/content/42/3/141.full.pdf+html (Please note, the wages listed are the median wages of everyone. And the average number of years that people were working that were reporting there wages was usually 10-15 years. So the wages reported are NOT the beginning salary.) Here's my summary of the vacancy rates: Histology Tech Vacancy Rate = 8.5% (second highest in survey) Histology Supervisor Vacancy Rate = 17.8% (3rd highest in survey) Histotech Overtime/Double shifts = 20.9% (second highest in survey) Time to fill histotech position: - 26.7% took less than 3 months - 34.2% took 3-4 months - 25.3% took 6-12 months - 13.7% took more than a year (highest in the survey) (That's why asking for hiring bonus or moving expenses works sometimes. Almost 40% take more than half a year to find someone!) Time to fill histology supervisor positions: - 26.0% took less than 3 months - 26.7% took 3-6 months - 23.3% took 6-12 months - 24.0% took more than a year (highest in the survey) Percent histotechs who will retire in next 5 years - 15.3% (2nd highest in survey) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -- From: Mary mhunt...@earthlink.net Sent: Thursday, April 21, 2011 6:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What’s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histologist looking for work in San Diego
I have 8 years clinical, 3 years pharmaceutical research. Well rounded (embedding, microtomy/cryotomy, special stains, IHC, ISH, Digital Pathology/Image Analysis, double-staining. Looking for position close to San Diego. Please contact me for CV or any leads. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
We also use Neutralex. Regards, Ben. Benjamin Ahlijah, BS Pathology Lab Supervisor BioReliance Corporation 14920 Broschart Road Rockville, MD 20850 Office: 301-610-2602 Fax:301-610 2199 benjamin.ahli...@bioreliance.com Please consider the environment before printing this e-mail BioReliance Corporation, Celebrating 60 years of contract bioservices This message is intended for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or if you have reason to believe you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you received this communication in error, please notify us immediately. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Thursday, April 21, 2011 12:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi from Southern Cali, We neutralize our formalin with a product called Neutralex. It comes in powder form and after 30 mins we can dump it down the drain. We also keep records for QC purposes. Product is purchased from a company called American MasterTech. Hope this gives you a starting place. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Input needed
Age and gender should not play a role in your chances of getting a job. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Sent: Thursday, April 21, 2011 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What's the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Neutralizing Formalin
We bought materials for neutralizing formalin and started to use them. Two years later, the local EPA authorities told us to stop because we lacked the competence to neutralize hazardous materials. Our neutralizing solution became a hazardous material. We now pay a waste hauler to take our formalin. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Thursday, April 21, 2011 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutralizing Formalin Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A survey of IHC lab staffing
Good morning, I am sending out one of those tedious surveys for people to answer if they feel so compelled and have the interest/time. Ours is an automated lab with Ventana instruments, 2 XTs and 1 BMK but I encourage anyone who wishes, to answer. We are currently moving from a 2 permanent FTE IHC lab to a 1 permanent FTE plus 1 FTE from a pool of 6 rotating surgical pathology techs. As you may have guessed, I am the 1 permanent FTE and I'm having a difficult time envisioning how this rotation of techs is going to work. These are my questions off the top of my head for now: * What is your monthly/yearly IHC workload? * How many techs rotate through IHC? * Are there any permanent techs in IHC that don't rotate? * Do all the techs, permanent or rotating, perform all the duties of the IHC lab? * What is your schedule of rotation? Daily? Weekly? Monthly? Other? * What are the hours of your IHC lab? The same as for the histology lab? * How many people are working in the IHC lab at any given time? If it varies, how many hours are one, two, more people staffing the IHC lab during the day? * Do you have more than one shift working in IHC? * Do you feel that all the people working in IHC have about the same level of expertise? If not, how is that dealt with? * Who keeps track of things like antibody, detection, ancillary reagent and supply inventories? * Does this same person do the ordering or do all techs order? * Who does monthly/quarterly cleaning and decontamination of your instruments? * Who is responsible for maintenance of temperatures, pH meter(s), freezer defrosting, making up of bulk solutions i.e. reaction buffer, EZ-Prep, etc. * Do all people, some, or one person deal with QC of new lots of antibodies, detection, etc? * Who is responsible for trouble shooting instrument/staining failures or problems? * Who is responsible for finding and maintaining control tissue inventory? * Who is responsible for workload tallies/recording? * Who is responsible for bringing on new antibodies? * Who is the main contact person with Ventana or other instrument/antibody vendor? * Who does send outs to reference labs for antibodies you don't carry? * Are your requests mostly by panels of antibodies or not? * Are certain antibodies/panels always requested on certain tissue types/diagnoses? * Are some/all/none of your IHC requests cut at the time of the HE sectioning? * What is your TAT policy for IHC requests, i.e. deadline for same day TAT, from in when to out when? * If you are a VMS automated lab, how many and which VMS instruments do you have? * Do you use all predilute, all concentrate, or some of each kind of antibody? Thank you for your time and input. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A survey of IHC lab staffing
Good morning, I am sending out one of those tedious surveys regarding staffing of IHC labs. Ours is an automated lab with Ventana instruments; 2 XTs and 1 BMK but I encourage anyone with the interest/time to respond. We are currently moving from a 2 permanent FTE IHC lab to a 1 permanent FTE plus 1 FTE from a pool of 6 rotating surgical pathology techs. As you may have guessed, I am the 1 permanent FTE and I'm having a difficult time envisioning how this rotation of techs is going to work. These are my questions for now: * What is your monthly/yearly IHC workload? * How many techs rotate through IHC? * Are there any permanent techs in IHC that don't rotate? * Do all the techs, permanent or rotating, perform all the duties of the IHC lab? * What is your schedule of rotation? Daily? Weekly? Monthly? Other? * What are the hours of your IHC lab? The same as for the histology lab? * How many people are working in the IHC lab at any given time? If it varies, how many hours are one, two, more people staffing the IHC lab during the day? * Does your IHC lab operate more than one shift? * Do you feel that all the people working in IHC have about the same level of expertise? If not, how is that dealt with? * Who keeps track of things like antibody, detection, ancillary reagent and supply inventories? * Does this same person do the ordering or do all techs order? * Who does monthly/quarterly cleaning and decontamination of your instruments? * Who is responsible for things like maintenance of temperatures, pH meter(s), freezer defrosting, making up of bulk solutions and antibodies, etc.? * Do all people, some, or one person deal with QC of new lots of antibodies, detection, etc? * Who is responsible for trouble shooting instrument/staining failures or problems? * Who is responsible for finding and maintaining control tissue inventory? * Who is responsible for workload tallies/recording? * Who is responsible for bringing on new antibodies? * Do you use predilute, concentrate or some of each type of antibodies? * Who is the main contact person with Ventana or other antibody/instrument vendor? * Who does send outs to reference labs for antibodies you don't carry? * Are your requests mostly by panels of antibodies or not? * Are certain antibodies/panels always requested on certain tissue types/diagnoses? * Are some/all/none of your IHC requests cut at the time of the HE sectioning? * What is your TAT policy for IHC requests, i.e. deadline for same day TAT, from in when to out when? * For VMS users, how many and which VMS instruments do you have? * Any opinions on which model of staffing has worked best in your situation? I will compile any results received and send it out on Histonet. Thanks for everyone's time and input, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sorry about duplicate IHC Lab staffing survey...please respond to only one
Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Neutralizing Formalin
We recycle most of our formalin using the Creative Waste system but if we have an especially dirty batch (usually from the monthly dumping of specimens) we neutralize it with Hyde-Away (Fisher cat. # 04-355-78) we pay $269 for a case of 4 gallons (uses 8oz.for each gallon of formalin). My lab is in Vermont and as far as I know we comply with the state regs. Hope this helps. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, April 22, 2011 9:14 AM To: Evans, Andria B Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Neutralizing Formalin We bought materials for neutralizing formalin and started to use them. Two years later, the local EPA authorities told us to stop because we lacked the competence to neutralize hazardous materials. Our neutralizing solution became a hazardous material. We now pay a waste hauler to take our formalin. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Thursday, April 21, 2011 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutralizing Formalin Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unsubscribe
Hi, I am the Histonet administrator and I just tried to remove the people requesting to leave the Histonet list but both addresses were not on the list so I guess they were successful in removing themselves. Anyone wishing to remove their address from the list or change something about their subscription (ie. update the address, switch to the daily digest version, put on a temporary vacation hold on the mail etc), please go to the website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and change your subscription using the instructions at the bottom of the page. Please remember, if your email address changes at all from when you first subscribed, you must update it on the list, in order to be able to post messages. If you cannot for some reason unsubscribe or have other problems, please let me know. Thanks, Linda M Histonet administrator -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 21, 2011 9:35 AM To: Emily Sours; Mollie Hannon; spanish_fly1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unsubscribe **Listserv master: Some people are reporting problems with managing their subscription as they have submitted their unsubscription multiple times in the prescribed manner and are still getting Histonet emails. This was reported to me by one such (failed) unsubscriber.** --On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours talulahg...@gmail.com wrote: Jupiter's thunder two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love to unsubscribe you so I didn't have to get your ridiculous emails about how you can't do it your damn self. A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6118 Fx: (716) 829-2665 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Positive controls
Hello Histoland, Does anyone have a good source for purchasing HSV I II ( Cocktail) positive controls? Thanks, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Input needed
Hi Jason, It is against Federal law to discriminate based on age or gender. And one should not have too much of an issue finding a job, many places are hiring and looking for well-trained techs. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 (Office) 615-875-3311 (Lab) 615-343-9134 Message: 16 Date: Thu, 21 Apr 2011 18:06:19 -0400 From: Mary mhunt...@earthlink.net Subject: [Histonet] Input needed To: histonet@lists.utsouthwestern.edu Message-ID: A17271D060CC4B8D8342DB154F1E0F4D@maryPC Content-Type: text/plain; charset=utf-8 What???s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Input needed
Out of 6 recent hires, I hired two 50+ employees. One had been employed in another department for over 28 years. One was a fairly recent grad. of a Histology program. They were both good employees, though neither had much routine Histology experience. That being said, neither one of them are still here: one chose to retire, after 3 years in our department. The other one decided to return to the previous place of employment within 6 months, stating that the fast pace of our lab was a deciding factor. Bottom line - neither age or gender played a role in their getting hired. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, April 22, 2011 8:11 AM To: Mary; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Input needed Age and gender should not play a role in your chances of getting a job. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Sent: Thursday, April 21, 2011 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What's the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Input needed
Hi Mary and Jason, I am a recruiter who works exclusively in permanent placement of histology professionals nationwide. Here in Orlando Keiser University has a great Histology program and I work with the new grads on job placement. What I have found is that the biggest challenge for a new grad in this economy is the lack of entry level positions. Most of the employers want someone with a minimum of 2 years of experience. I blame it on the economy because 3 years ago I placed as many new grads as experienced techs and nowadays it is very difficult not only for me to place a new grad but for the new grads to find positions themselves. Most labs seem to be shortstaffed so they can't take the time to supervise a new grad. I suggest that you contact the career placement center of the school that has the histology program that you are thinking about attending ask them what their placement rate is for histotechs. Be sure and find out not only how many of their techs get jobs but how many get jobs in the local area and how many needed to relocate to find employment. If there is not a career placement center I suggest that you talk with the program director or one of the instructors. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: endogenous peroxidases
Hi Emily, As for when is better to block endogenous peroxidases, I have no direct experience but I have heard from some that it should be done post-AR because the retrieval can reactivate the endogenous peroxidase. So that's when we do it. Some folks worry about what effect that might have on their primary antibody binding with their target and will do their block after application of the primary antibody, prior to the secondary (or whichever step has the HRP label). I try to keep it simple and just do my H2O2 block prior to the protein blocking step. Also, we never use methanol as a solvent for diluting the H2O2. It is contraindicated in some CD marker staining, and it's cheaper and just as good to use aqueous 3% H2O2. Some make it up in buffer, and you can do that as well if you wish. For cryosection peroxidase quenching, we use 0.3% H2O2 for 30 minutes. Otherwise it's a 10 minute step right after the AR in our routine paraffin section IHCs. Hope this helps! Teri Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Positive controls
Hi Cindy, We get ours from American Master Tech: HSV1 Item # CSH0425P (Box of 25) HSV2 Item # CSH0525P (Box of 25) I use Cell Marque's antibodies and they cross-react with each other, so you should be able to get by with one or the other. (I would test this in your lab first, though.) Good luck. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 (Office) 615-875-3311 (Lab) 615-343-9134 Message: 13 Date: Fri, 22 Apr 2011 07:12:48 -0700 (PDT) From: Cindy Bulmer cjbul...@sbcglobal.net Subject: [Histonet] Positive controls To: Histonet histonet@lists.utsouthwestern.edu Message-ID: 988711.32712...@web82302.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hello Histoland, ? Does anyone have a good source for purchasing HSV I II ( Cocktail) positive controls? ? Thanks, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] endogenous peroxidases
Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: Gimme 5! Block before or after Ag retrieval. If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 in Dist.water. Block for 15mins. Methanol is not neccessary...imho. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus London SE1 1UL Tel: 020 78486813 Fax: 020 78486816 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] endogenous peroxidases
So I have been seeing from this post that people block prior to HIER? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker). Is this wrong? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, April 22, 2011 12:42 PM To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] endogenous peroxidases Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: Gimme 5! Block before or after Ag retrieval. If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 in Dist.water. Block for 15mins. Methanol is not neccessary...imho. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus London SE1 1UL Tel: 020 78486813 Fax: 020 78486816 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo lab consultants
Dear Histoneters, We are looking for histotechnologists or lab consultants to talk to who are currently working in labs that may have a need for anatomic pathology services. If so, we can make it worth your while. Please give me a call or send me an email: a.byr...@accelpath.com 732-312-8008 www.accelpath.com Thanks! Andrew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Howdy all
Happy Friday Histo peeps Would anyone know of any openings for Histo techs in the Southern Utah area? I have a tech working with me that is looking to relocate from Alaska to Utah to be closer to family. Any info would be great. Thanks Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] endogenous peroxidases
My sequence is: dewax (with dishwasher soap) → wash → HIER → wash → block endogenous peroxidase → go tthe IHC protocol. René J. --- On Fri, 4/22/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com sgoe...@mirnarx.com Subject: RE: [Histonet] endogenous peroxidases To: akemiat3...@yahoo.com, carl.ho...@kcl.ac.uk Cc: histonet@lists.utsouthwestern.edu Date: Friday, April 22, 2011, 1:50 PM So I have been seeing from this post that people block prior to HIER? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker). Is this wrong? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, April 22, 2011 12:42 PM To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] endogenous peroxidases Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: Gimme 5! Block before or after Ag retrieval. If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 in Dist.water. Block for 15mins. Methanol is not neccessary...imho. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus London SE1 1UL Tel: 020 78486813 Fax: 020 78486816 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL positions open at UNC Hospitals
Hi Histonetters!! UNC Hospitals is currently searching for: A Histotechnologists proficient in all aspects of routine Histology, including tissue processing, embedding, sectioning, and maintenance of equipment. In addition, advanced skills in Muscle processing and Histochemistry, Immunohistochemistry, special stains and frozen tissue sectioning are preferred. Requirements include a minimum of one (1) year of histology experience, with HTL (ASCP) certification or eligibility as defined by the ASCP. Uncertified candidates must obtain certification within 18 months of hire. Various shifts available. Scheduled work hours may vary for training purposes and departmental coverage needs. A Histotechnologist for Electron Microscopy and Muscle Pathology services. The HTL-EM/Muscles must be technically proficient in all aspects of clinical electron microscopy. Skills required include tissue collection, freezing, fixation, processing, plastic embedding, block trimming, ultra-microtomy, staining, EM scanning and micrography. Experience with muscle pathology or routine histology procedures is a plus. This individual must be able to work independently and function with minimal supervision. Work hours are Monday-Friday, with varying start times ranging from 6:00 AM to 8:00 AM depending on EM scope availability and case volume, with occasional rotating Saturdays. Scheduled work hours may vary for training purposes and departmental coverage needs. To apply or learn more about our career opportunities, please visit our website at www.unchealthcare.org/jobs. Sheila L Deloney Histology Laboratory Supervisor UNC Hospitals/ McLendon Clinical Labs 101 Manning Drive WC Hospitals RM CH30231 Chapel Hill, NC 27514 Ph: 919-843-1082 / 966-6288 fax Pager: 919-347-1677 Email: sdelo...@unch.unc.edu mailto:sdelo...@unch.unc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A PRN Histotech Needed in Charleston, SC
I have an open PRN HT position open at Trident Medical Center in Charleston, SC. The website to apply is TridentHealthSystem.com under careers/For Professionals/search jobs/apply online. The job # is 00062-8978. Ideal hours would be flexible if possible (mornings or afternoons), but priority is early morning to help with microtomy. Great crew to work with and good equipment (2 Shandon's and 2 Microm's) Special rate for PRN HT. Description states BS degree and NACCLS program, but it should read or NACCLS program. Call me below with question or apply on-line. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
