[Histonet] Histology Job Opportunity
Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Histotech position in Western GA (will relocate): I am currently working on an amazing opportunity with an award winning non-profit community hospital in western GA. This well-respected 400 bed hospital is looking to hire on a histotech. They are looking for either an experienced histotech or new graduate who is HT(ASCP) certified. The compensation package is fantastic and includes a competitive hourly rate comprehensive benefits package, tuition reimbursement, and relocation, if needed. If interested in learning more details, please email me at ali...@ka-recruiting.com. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Cytotech and Histotech 1. MI - Histology Manager 2. NY - Rochester - Histotech 3. FL - Jacksonville - Histotech 4. NH - Histology Supervisor 5. NC - Pathology Lab Manager 6. GA - Histology Supervisor 7. ME - Histotech 8. FL - Histotech 9. NY - Histotech 10. NY - Grossing Tech 11. NY - IHC Tech 12. NY - IHC Supervisor 13. NV - Histotech 14. IL - Chicago - Histotech 15. Texas If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C4d stain for IF
Patrick, or anyone else using the alpco product...can you give some details/feedback on how this anti human C4d (FITC-Conjugated) is working for you, and whether you were able to replace two steps with one for C4d IF staining? I checked that website, and the pdf on this product reads: The antibody detects human complement split product C4d and has been tested for use on paraffin sections and frozen sections of human Tissue. We've switched our goat anti-mouse currently used for C4d immunofluorescence, and would prefer to switch to a conjugated product. It sounds like something we'd like to try. Thanks in advance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada Try the C4d directly labeled antibody from alpco www.alpco.com anti human C4d (FITC-Conjugated) Cat #: 04-BI-RC4D-FITC Good luck. On Wed, Apr 27, 2011 at 2:54 PM, Butler, Roszetta rbut...@ameripath.com wrote: I'm having trouble with my C4d IF stain. I'm using AbDirect/Serotec, mouse monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec diluted 1:25 for ½ hour. This is not working and I've tried different dilutions with no success. Can anyone share their protocol? Rosie Butler, AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK 73112/405.842.7575 - Office 405.650.8921 - Cell/405.841.2002 - Fax -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opportunities
Does anyone know of Histology opening in the Kansas City area? I know of an individual who is looking. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cap new ANP.22760
ANTIBODY LOTS I have a Dako and I have a Bond too. For the Dako I use my positive control. I use 3 slides. One is treated as the negative control - no antibody One is treated with the current lot One is treated with the new lot. I keep all QC slides together. I keep the 3 slides together. For my Bond, I use 2 slides of my positive control When a new lot comes in I run the new lot and the negative control. Then I compare them to the previous lot that I ran in the past. I keep the slides in chronological order - the previous lot goes back with The slides from that date. The NEW Lot slides get filed with today's date. DETECTION SYSTEM LOTS For the Bond I QC the detection kit using an antibody like Ki-67, it works well and always shows good nuclear staining. I indicate the received date, and QC date. I run the QC on my Ki-67 positive control. I use 2 slides. One is treated as the negative control - no antibody. One is treated with Ki-67. I compare these to the previous Detection kit QC slides I created a written procedures for all of the above. Hope this helps. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Friday, April 29, 2011 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap new ANP.22760 hi everyone, just wondering how others were handling this checklist statement? thanks so much for your input. seems like a lot of extra work when running control with each show if each is working. anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pax-2 on FFPE human tissue
Hi All, Having a tough time looking for a reliable vendor for Pax-2. What vendors are you using? I need to use this on formalin fixed paraffin embedded human tissue. What control do you use? Thank you. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Could someone please tell me the requirements in work in Germany
Hi All, I would greatly appreciate if someone could tell me the requirements for working in Germany as a histologist? Is it hard to get work? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pax-2 on FFPE human tissue
Hi Dana, Invitrogen sells a rabbit polyclonal anti-pax-2 antibody that works well on FFPE tissues (Cat # 180483). We use a multi-tissue control block for a control. It has normal kidney to show the endothelial cells around glomeruli and renal tubules staining, clear cell renal cell Ca to show tumor staining and a small piece of tonsil to show that B-cells stain. Hope I helped, Mark On Fri, Apr 29, 2011 at 11:04 AM, Settembre, Dana sette...@umdnj.eduwrote: Hi All, Having a tough time looking for a reliable vendor for Pax-2. What vendors are you using? I need to use this on formalin fixed paraffin embedded human tissue. What control do you use? Thank you. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] special stain controls
Hi, I am in need of control blocks for special stains, H. Pylori, pneumocystis and AFB. I am attempting to grow our own AFB, but am having difficulty getting the other control blocks. Commercial vendors charge a LOT of money for these controls! I was hoping to find a way to swap controls, if I could. Does anyone have any suggestions for me? I don't mind buying them, but tend to cringe when the costs are around $10/slide!! Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tumors tumors everywhere
Did you check to ensure the secondary in the kit is anti-rabbit IgG, with minimal cross to mouse? Ask Biocare directly. What does the background look like? What does your isotype look like? How about your secondary only control? Sent from my Verizon Wireless BlackBerry -Original Message- From: sgoe...@mirnarx.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 28 Apr 2011 15:16:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tumors tumors everywhere So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] special stain controls
I can provide you with all the H. pylori you could ever want if you have some good fungus blocks. Let me know if you'd like to trade! Drew Sent from my iPhone On Apr 29, 2011, at 6:20 PM, histot...@imagesbyhopper.com wrote: Hi, I am in need of control blocks for special stains, H. Pylori, pneumocystis and AFB. I am attempting to grow our own AFB, but am having difficulty getting the other control blocks. Commercial vendors charge a LOT of money for these controls! I was hoping to find a way to swap controls, if I could. Does anyone have any suggestions for me? I don't mind buying them, but tend to cringe when the costs are around $10/slide!! Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
