[Histonet] (no subject)
http://www.xstreamwd.com/wp-content/themes/default/work.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] modified Gallyas stain for microglia
Hello, I'm using the modified silver stain of Gallyas (J. Neurosci. Methods 50:159--164, 1993) for microglia. It didn't work first time round so I'm doing some trouble shooting. My main concern is the shelf life of the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL H2SO4 per 100 mL). It is now a nice green color and looks a bit darker than yesterday. Anyone know for how long this solution is good? If anyone else has used this method I would appreciate if you could share your experience. If anyone can suggest another chemical staining protocol for microglia which works against tissues of various species I would be grateful. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
...Ive got something for you! http://www.sweep.co.il/page.php?jcyhotmailID=07o5 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] motorized cryostat
In spite of the apparent advantage, FS are so uniquely demanding while sectioning, that the manual touch could never be substituted by an automatic feeding mechanism. Buy a good manual cryostat. I always used Leica. Ask for a demo. René J. From: Ed Roy e...@illinois.edu To: histonet@lists.utsouthwestern.edu Sent: Friday, May 13, 2011 5:55 PM Subject: [Histonet] motorized cryostat I have a small research lab with an old (very old) Minot IEC microtome cryostat, which is on its last legs. In starting to shop for a new one, I was wondering how much difference a motorized one would make. We do mostly fresh frozen (unfixed) mouse brain and lymph node sections. Are the sections any better? Thanks for any opinions on this and any recommendations on a good cryostat at a reasonable cost. Ed Roy -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histology station for core lab
For teaching you are right that the path is manual. For a limited budget Google for used microtomes or try eBay. René J. From: Debra Baluch page.bal...@asu.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, May 13, 2011 7:16 PM Subject: [Histonet] Histology station for core lab Hi All, I manage a bioimaging core facility and teach a couple of courses related to cell and tissue culture research and sample prep for live and fixed cell imaging. We have expanded to train people on the use of a cryostat, that is part of our core facility, and are now looking into obtaining a microtome to teach histological techniques. Do you have recommendations of a good rotary microtome that would work well in a core facility? Most researchers in our department manually embed and stain so we are not as familiar with the more automated instruments for sample processing. Since much of the focus is in regards to learning the process we are leaning towards going with the more manual route. We are working with a limited budget so we will need to be somewhat conservative in the models we select. Thanks for your suggestions, Page ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re:Hi. Alice asked me to help you to stop smoking! Dont bother! I know how to help you!
...You will get all you need just sitting at home and eating popcorn! http://obermaneken.ru/page.php?iyahooID=16my7 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: modified Gallyas stain for microglia
Tyrone Genade asks: I'm using the modified silver stain of Gallyas (J. Neurosci. Methods 50:159--164, 1993) for microglia. It didn't work first time round so I'm doing some trouble shooting. My main concern is the shelf life of the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL H2SO4 per 100 mL). It is now a nice green color and looks a bit darker than yesterday. Anyone know for how long this solution is good? The spelling ferroscyanide doesn't exactly inspire confidence - do you mean ferricyanide? John Kiernan did a very nice review of Gallyas stains (of which there are apparently several) on Histonet about three years ago. See http://www.histosearch.com/histonet/Apr08A/Re.HistonetGallyasStain.html John Kiernan describes these methods as quite difficult. I have no experience with them. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Outdating- Embedding Wax and Mounting Media
To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their continued use beyond this date. I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outdating- Embedding Wax and Mounting Media
Now you guys have done it!!! All the QAQC gurus are thinking!!! Rae Staskiewicz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Saturday, May 14, 2011 4:56 PM To: John Smallwood; Histonet Subject: Re: [Histonet] Outdating- Embedding Wax and Mounting Media How funny! You are so right! What about the ink on all of those old reports and reference books? Isn't ink a dye in solvent? The best special stains I ever did were back in the day when I made my own solutions from dry chemicals that were already 20 to 30 years old. Paperwork, just a way to decrease productivity! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: John Smallwood jrsmallw...@bell.net To: Histonet Submissions histonet@lists.utsouthwestern.edu Sent: Sat, May 14, 2011 4:18:36 PM Subject: [Histonet] Outdating- Embedding Wax and Mounting Media To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their continued use beyond this date. I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
