Fw: [Histonet] CD79 alpha dilemma - ANSWER CORRECTION
Hello everyone - my apologies for posting a partially incorrect answer last week. I was looking at the wrong line on a spreadsheet for CD79a abbreviated protocol information and wrote down the wrong HIER information. What we do here currently with HIER for Biocare's CD79a (clone HM47/A9) on dogs, cats, pigs, cows, etc., is unmask antigens using: 0.1M Citrate buffer, pH 6.0 in a Biocare Decloaking Chamber. (High pH retrieval solution does expose more antigenic sites, but it damages the tissue more when using the pressure cooker method.) In the past we used high pH retrieval solution when doing HIER using a microwave oven (gentler method). Jan Shivers UMN Vet Diag Lab - Original Message - From: Jan Shivers shive...@umn.edu To: Farish, Craig cfar...@csu.edu.au; histonet@lists.utsouthwestern.edu Sent: Friday, May 13, 2011 9:14 AM Subject: Re: [Histonet] CD79 alpha dilemma Hi Craig, I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, horse, sheep, and mouse so far. Have not tried it on other species yet (in other words, I don't have any negative species results to report). I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to expose antigens (in a Biocare Decloaking Chamber/pressure cooker). Citrate buffer doesn't do the job well enough. Microwave oven antigen retrieval will also work, but it exposes fewer epitopes than the pressure cooker. If you have any more questions, feel free to contact me directly. Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Farish, Craig cfar...@csu.edu.au To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 8:35 PM Subject: [Histonet] CD79 alpha dilemma Hi folks - has anyone out there found a Cd79α clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] motorized cryostat
We have a motorized and rarely use the motorized function, Maybe 5 time in the last 5 years. It is faster to cut manually. Donna Reynolds, chief Histology research lab U.T. M.D. Anderson Cancer Center, Houston, TX 713-792-8106 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] chemical waste co
We work with Stericycle, and they are great! I've worked with them for years. They pick up all of our chemical and biohazard waste and also separate the formalin from the specimens for disposal. Their number is (323) 266-6448. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rmweber...@comcast.net Sent: Monday, May 16, 2011 5:32 PM To: histonet Subject: [Histonet] chemical waste co Hello, Does anyone have a name of a laboratory chemical waste company in Southern California? Thanks for your help. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Tissue-tek Glas Air bubble problems
Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Tissue-tek Glas Air bubble problems
Make sure the needle is clean - soak every night. And be sure your coverslips are clean. Look closely - there may be ground glass on the coverslips.. Then kiss it some more...:) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lees, Sara Sent: Tuesday, May 17, 2011 10:18 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Tissue-tek Glas Air bubble problems
If you are using one of the older film coverslippers there is a roller following the application of the film. Make sure that roller is still in the correct place or that one of the springs is not missing or in need of replacing. The job of that roller is to squeeze the air bubbles from under the film. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lees, Sara Sent: Tuesday, May 17, 2011 9:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems
Thin the mounting media with xylene or solvent you use. I use Permount thinned 60/40 with xylene. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Lees, Sara sara.l...@covance.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Freezing rodent brain
How is everyone freezing hippocampus sections? The current method being used seems to be causing a lot of artifact. Thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Freezing rodent brain
It must be completed in seconds. Buried in powdered dry ice is one way. Immersion in a slurry of dry ice and isopentane is another. Cordially, Charles W. Scouten, Ph.D Product Specialist, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com http://www.myneurolab.com/ www.leica-microsystems.com http://www.leica-microsystems.com/ IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone a...@buffalo.edu Sent: Tuesday, May 17, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing rodent brain How is everyone freezing hippocampus sections? The current method being used seems to be causing a lot of artifact. Thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems
Are you using the original Sakura film? René J. From: Lees, Sara sara.l...@covance.com To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, May 17, 2011 10:17 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems
Sara, The same problem with air bubbles developed for me as well. I hadn't changed anything but it slowly got worse. On a hunch I changed the xylene substitute and it corrected everything. Can't say that I know why, but I my guess was that the miscibility with the mounting media was becoming worse. The shelf life was still good on the mounting media but it had been in stock for some time. I don't know if it makes any difference but I cover slipping manually. Of course, it was our first guess that someone was being inattentive while cover slipping but it became clear that that wasn't the case. good luck, Jason On Tue, May 17, 2011 at 7:17 AM, Lees, Sara sara.l...@covance.com wrote: Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Filter Paper for Harris Hematoxylin
Good morning, What grade filter paper do you use for purchased Harris Hematoxylin filtering? I have used 3 different grades, coarse and still have precipitate on my slides. Any advice is greatly appreciated. Thank you Karie -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:krea...@vet.upenn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Tissue-tek Glas Air bubble problems
Try an extended prime when you start it in the am, it seemed to resolve the issue with our glass coverslipper at least for now. (We were having a horrible time with it, and that's what the repair man did. No bubbles for five days and running!) Erin G. Bertani, B.A. Clinical Laboratory Assistant Department of Pathology and Laboratory Medicine Roswell Park Cancer Institute Elm Carlton Sts Buffalo, NY 14263 Phone: 716-845-3145 Fax: 716-845-2370 erin.bert...@roswellpark.org -Original Message- From: Paula Pierce [mailto:cont...@excaliburpathology.com] Sent: Tuesday, May 17, 2011 10:37 AM To: Lees, Sara; Histonet Subject: Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems Thin the mounting media with xylene or solvent you use. I use Permount thinned 60/40 with xylene. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Lees, Sara sara.l...@covance.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Sakura Tissue-tek Glas Air bubble problems
Try an extended prime when you start it in the am, it seemed to resolve the issue with our glass coverslipper at least for now. (We were having a horrible time with it, and that's what the repair man did. No bubbles for five days and running!) Erin G. Bertani, B.A. Clinical Laboratory Assistant Department of Pathology and Laboratory Medicine Roswell Park Cancer Institute Elm Carlton Sts Buffalo, NY 14263 Phone: 716-845-3145 Fax: 716-845-2370 erin.bert...@roswellpark.org -Original Message- From: Paula Pierce [mailto:cont...@excaliburpathology.com] Sent: Tuesday, May 17, 2011 10:37 AM To: Lees, Sara; Histonet Subject: Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems Thin the mounting media with xylene or solvent you use. I use Permount thinned 60/40 with xylene. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Lees, Sara sara.l...@covance.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job Opening in Fort Myers, FL 3rd Shift
Allied Search Partners is currently looking for a qualified applicant for a Histotechnologist or Histotechnician willing to work the third shift within a Fort Myers, FL laboratory. Position: Histotechnologist or Histotechnician Schedule: Monday-Friday, Overnight hours/Third Shift Summary: Perform a variety of routine and specialized histology techniques and procedures. Embedding, Microtomy, Grossing, Processing, and HE staining Special Staining Equipment maintenance Requirements: FL Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science preferred but not required Benefits: Competitive salaries, Health, Dental, Life Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To Apply: Please send resume to aly...@alliedsearchpartners.com mailto:bran...@alliedsearchpartners.com Alyssa Peterson, Candidate Recruitment LinkedIn: http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ http://www.alliedsearchpartners.com/ This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Kim Tournear
Need to speak to Kim Tournear and I don't have her contact info. Kim, if you are reading this - call me. 520.626.4415 Andi Grantham ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin temps
Could someone please share with me their standard for setting an acceptable temperature range for the paraffin stations on the processors? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin temps
Each paraffin bag states the melting point temperature of the contained paraffin. Just use that value +1ºC René J. From: Artim, Kimberly art...@slhn.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, May 17, 2011 11:57 AM Subject: [Histonet] Paraffin temps Could someone please share with me their standard for setting an acceptable temperature range for the paraffin stations on the processors? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Filter Paper for Harris Hematoxylin
Hi Karie Whatman No: 1 Qualitative Grade should effectively remove all precipitate and leave you with a fresh clean staining solution. Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter Devon. EX1 3AJ Tel: +44(0)1392) 660132 On 17 May 2011 15:51, Karie Reaser krea...@vet.upenn.edu wrote: Good morning, What grade filter paper do you use for purchased Harris Hematoxylin filtering? I have used 3 different grades, coarse and still have precipitate on my slides. Any advice is greatly appreciated. Thank you Karie -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:krea...@vet.upenn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Hot Histology Job Alert. Managers, Experienced Techs and New Grads...Check it out!!
Hi Histonetters!! I hope everybody is having a great day. I have a couple of jobs I want to tell you about. These are permanent full time positions and my clients offer great compensation, benefits and relocation assistance. Here are the jobs I want to tell you about: Histology Management: **Asheville, NC **Boston, MA **Baton Rouge, LA Histology Technicians/Technologists: **Long Island, NY - day shift NY license or elig, NEW GRADS WELCOME! **Sarasota, FL - Mohs Tech **Ft. Myers, FL - Dermpath Tech **Portland, OR - Histotech **Portland, ME - Histotech I also need a PA in Charlotte, NC If you or anyone you know is interested in more information about any of these opportunities please contact me at mailto:rel...@earthlink.net rel...@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: mailto:rel...@earthlink.net rel...@earthlink.net http://www.facebook.com www.facebook.com search Pam Barker RELIA http://www.linkedin.com/reliasolutions www.linkedin.com/reliasolutions http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia http://www.twitter.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fixation and Processing of Mouse Lactating Mammary Gland
Hello to all, I have some lactating mouse mammary tissue that was fixed in 10% formalin for 24 hours and then stored in 70% alcohol for 1 day before processing. The processing is as follows: 80%-30min, 95%-30min, 95%-40 min, 100% X3-30 min. each, xylene X3-40min. each, and paraffin X3-45min. each. The tissue if very mushy and I am wondering if the tissue was fixed long enough? The tissue is approximately 3mm in thickness but because of the milk in the tissue does it need to fix more or should I process longer? I have embedded the tissue and am wondering how I should go about reprocessing the tissue. Should I go back through xylene to alcohol and then fix for longer and then reprocess or can I melt the blocks and put in fix and then reprocess? I have heard both ways but want to do the method that will not comprise the IHC that needs to be done. Thank you so very much for all of your input and help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edumailto:pat.b...@ucdenver.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ihc on mouse femour
hai histonetter, i am doing ihc on mouse femour(bone marrow),i am using nucleostemin antibody to locate cells in proliferation, can any one tried this if u tried please give me the idea about nucleostemin ihc on mouse femour, thank u. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] correct CPT code for anal smear
What would be the correct CPT code for an anal smear collected in a SurePath vial (thin layer technology) for cytological evaluation and subsequent HPV testing? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fixation and Processing of Mouse Lactating Mammary Gland
I think longer fixation AND processing. Fix for at least 48 hours in NBF and here is my processing schedule 70% for 45 min 70% for 45 min 95% for 45 min 95% for 45 min 100% for 45 min 100% for 45 min 100% for 45 min xylene for 45 min xylene for 45 min xylene for 45 min paraffin for 45 min paraffin for 45 min paraffin for 45 min paraffin for 45 min Good luck On Tue, May 17, 2011 at 1:14 PM, Bell, Pat pat.b...@ucdenver.edu wrote: Hello to all, I have some lactating mouse mammary tissue that was fixed in 10% formalin for 24 hours and then stored in 70% alcohol for 1 day before processing. The processing is as follows: 80%-30min, 95%-30min, 95%-40 min, 100% X3-30 min. each, xylene X3-40min. each, and paraffin X3-45min. each. The tissue if very mushy and I am wondering if the tissue was fixed long enough? The tissue is approximately 3mm in thickness but because of the milk in the tissue does it need to fix more or should I process longer? I have embedded the tissue and am wondering how I should go about reprocessing the tissue. Should I go back through xylene to alcohol and then fix for longer and then reprocess or can I melt the blocks and put in fix and then reprocess? I have heard both ways but want to do the method that will not comprise the IHC that needs to be done. Thank you so very much for all of your input and help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edumailto:pat.b...@ucdenver.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin blocks from cell line
Hi histonetters, Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. Thanks in advance for the help Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 100 micron paraffin sections?
Hi Everyone: A researcher in our building has done a Golgi-Kopse silver stain on some brain tissue. He says that the tissue has come out brittle with this particular Golgi method. His pieces are 3 mm thick and will easily fit inside of a processing cassette. He would like to embed the tissue in paraffin and cut 100 micron sections. Does anyone have a protocol for processing this kind of tissue into paraffin and then sectioning and mounting such thick 100 micron sections? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD169 for paraffin embedded tissue
Hi, I am trying to stain paraffin embedded tissue with CD169, once antigen retrieval has been performed(heat and pressure), but have not have much success. I was wondering if anyone had had any success on paraffin embedded tissue using any specific Ab? Regards, Dr. Otto Strauss University of Auckland ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Expiry date of NBF
Dear Histonetters, I require one clarification on declaration of expiry date of 4% nutral buffer formalin (NBF) prepared in our lab ( from 40% solution) ? Do you declare the expiry date of this prepared solution ? If so what criteria do you follow for this? Important to mention that we have to archive these tissue in NBF for 5 years as part of GLP toxicology. Your experience or view on these points are welcomed. Amita ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Filter Paper for Harris Hematoxylin
Just continuation of karie query .Do you suggest different filter paper for Lab prepared hematoxylin? What's your view on lab prepared versus ready made Harris hematoxylin? Dr. Amita Dubey PCSED,TRC From: Karie Reaser krea...@vet.upenn.edu To: histonet@lists.utsouthwestern.edu Date: 17/05/11 08:27 PM Subject:[Histonet] Filter Paper for Harris Hematoxylin Sent by:histonet-boun...@lists.utsouthwestern.edu Good morning, What grade filter paper do you use for purchased Harris Hematoxylin filtering? I have used 3 different grades, coarse and still have precipitate on my slides. Any advice is greatly appreciated. Thank you Karie -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:krea...@vet.upenn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] congo red staining method for abdominal fat aspirates
dear colleagues, I have a problem with congo red staining of abdominal fat aspiration slides.i perform the same way as for slides prepared from paraffin-embedded blocks, but the results are poor. thank you in advance for your help. sincerely, sabeti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
