RE: [Histonet] VENTANA ULTRA ER,PR,HER2
Hi Antoinette, We currently use Ventana's ER and PR on both XT and Ultra. From what I understand, the antibody dispenser is labeled IVD. We report all cases as being 'approved by the FDA for IVD use'. We do perform correlation/precision studies twice a year. For this study we run five cases on both the XT and an ultra and document that the result comes out the same. I have actually found that this is a compliance issue with Joint Commission. I can try to find the reference if you need it. It states that if you are running the same test on two different platforms, you must prove correlation. As far as the Her2 dual ISH, we are in the process of evaluating it now. I see it as a definite advantage that it is bright field, permanent, and has the potential to dramatically increase TAT. We are in the middle of a cost analysis. On the other hand, we currently run Pathvysion and I see that some literature calls FISH and pathvysion the 'gold standard'. This is the most highly scrutinized test we perform considering the CAP/ASCO guidelines. My gut also tells me...why take a chance with this when our current system is not 'broken'? I did find that CAP offers two mailings for profiency testing using bright field ISH. Let me know how you make out. Hope this helps. Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, June 28, 2011 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
Stella, Please keep us informed as to the outcome of your discussions with management. I hope it all works out in your favor. I have also been in the field over 35 yrs. and have never been known as one of the fastest sectioners, but I always focused on quality and thought about the patient behind the specimen. As we all know there are more skills to being a great technician than just the ability to section fast. I used to love doing special stains when I worked in clinical and my slides reflected that. I think we can all agree that working with a well rounded, conscientious tech who may not be the quickest in the field, but is a great team member, is a much more desired and valuable employee than someone who can section 60 on up blocks/hr. yet is not a very good team player. I once went from working in research to taking a position in a small hospital that employed two techs. Both had left, so I walked into an empty lab ( they got by by having someone come in at night) and had to do everything myself including cyto preps. I'm sure I never managed 60 blks/hr but I got all the surgical done in a reasonable time. So good luck, and keep your options open, you may still want to look around for a position where the management understands histology. Seems there are lots of those places from all of the responses. Now about retiring to New Mexicoor that histo retirement community..count me in! Dolores -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, June 28, 2011 9:21 PM To: Delossantos_Roseann Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per To All I Really Appreciate All The Great Info. I am fully armed and ready to present my case to management. The knowledge among everyone out there is incredible. Thanks. Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quenching
Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Quenching
Candice: Quenching is usually done in IHC (immuno-histo-chemical) procedures and is intended to eliminate the reaction of the peroxidase (enzyme) present in all cells. If this step is not done the background (noise) in the form of dark color present in the final section could mask the actual IHC reaction. Go to the DAKO website and download a free IHC manual that can explain you this and any other aspect of the IHC general procedure. René J. --- On Wed, 6/29/11, Candice Smoots candice_cami...@yahoo.com wrote: From: Candice Smoots candice_cami...@yahoo.com Subject: [Histonet] Quenching To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, June 29, 2011, 11:20 AM Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Could you please recommend?
Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your positive and negative experiences with the models that you own. Which ones we should stay away from and which ones that you did not regret that you purchased it. Also some pricing details would be helpful. There were some earlier postings on this topic earlier which I did not bother to save. Thank you all in advance for your anticipated feedback. Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramu...@nrc-cnrc.gc.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Could you please recommend? (Bascaramurty, Saro)
We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well and was the first one that the anti-roll plate would work correctly. It decontaminates well. No regrets, love it and would recommend. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Could you please recommend? (Bascaramurty, Saro)
I second the Leica! :o) Michelle Sent from my iPhone On Jun 29, 2011, at 1:14 PM, White, Lisa M. lisa.whi...@va.gov wrote: We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well and was the first one that the anti-roll plate would work correctly. It decontaminates well. No regrets, love it and would recommend. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtome blade donations
Hi, One of our pathologists is going on a volunteer mission to a pathology lab in Malawi, Africa in a few weeks, and he is looking for donations of low profile microtome blades for the histology lab there. He said they will need around 200 blades or so, but any amount would help. He leaves on July 21st, so we are under a bit of a time crunch. Thanks! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing for CD3/20
Hey all, We are looking at doing the double stain CD3/20 and wanted to know how everyone is billing for it. Can we bill for 2 immuno stains? Or do we have to bill for one? What is the proper CPT for doing double or even triple staining? We are a Medicare facility. Thanks Matt Lunetta BS HT(ASCP) Longmont United Hospital Longmont, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Quenching
Quenching is also a term used with depressing fluorescence activity. This can happen in quantitative realtime-PCR procedures. Here a quencher is situated close to a fluorochrom and therefore the activity is low. During incorporation of the nucleotides the quencher is released and the fluorochrom-activity is restored. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Candice Smoots Gesendet: Mittwoch, 29. Juni 2011 17:21 An: Histonet Betreff: [Histonet] Quenching Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: is out of the office. (returning Mon 07/11/2011)
I am out of the office from Wed 06/29/2011 until Mon 07/11/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mi...@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewe...@leica-microsystems.com, for customer service support. Note: This is an automated response to your message Histonet Digest, Vol 91, Issue 41 sent on 6/29/2011 11:55:41 AM. This is the only notification you will receive while this person is away. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Could you please recommend? (Bascaramurty, Saro)
Not sure about the UV thingy but I love the Leica 1850 or the 1510 works great. But, if I had to choose between the two I would go with the Leica 1850.. You can purchased a used cryo for about 12-15,000. Most times with a one year warrenty. I recommend calling Margaret at IMEB for a quote. She is wonderful and will find what you need. Nicole Tatum HT(ASCP) I second the Leica! :o) Michelle Sent from my iPhone On Jun 29, 2011, at 1:14 PM, White, Lisa M. lisa.whi...@va.gov wrote: We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well and was the first one that the anti-roll plate would work correctly. It decontaminates well. No regrets, love it and would recommend. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome blade donations
Do they take volunteer histos, :) I would love to do somethig like that. Nicole Hi, One of our pathologists is going on a volunteer mission to a pathology lab in Malawi, Africa in a few weeks, and he is looking for donations of low profile microtome blades for the histology lab there. He said they will need around 200 blades or so, but any amount would help. He leaves on July 21st, so we are under a bit of a time crunch. Thanks! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position Alert - Field Histology Tech Southern CA
Good Afternoon Histonet, One of our retained Cancer Diagnostics clients has opened up an Field Technical Support Opportunity based in Southern CA. This position is perfect for a Histotech looking to get out of the lab and into the field. Field Support Specialist - IHC The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Position offers: Competitive Base Salary + Bonus/Commission + $700/Month for a Car Allowance and Gas Card, Cell Phone, Laptop, 401k, Full Benefits Open due to Promotion!! Please send your resumes to m...@personifysearch.com, or call me at 800.875.6188 ext. 103 Also, if you know of anyone who may be interested please do not hesitate to forward this e-mail, and have them reach out to me. Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: The results of your email commands
Hi Histoland, Just wanted to give a quick update to a post I replied to a few weeks ago regarding floater contamination and it's relation to the Peloris processor. We've worked with Leica (thanks Cindy) and to reiterate what I stated in my response our source of contamination was most likely the grossing station or embedding center. Lisa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plastic processing for undecalcified mouse bones
Hello, All, I am looking into starting a plastic embedding/sectioning endeavor, and am trying to figure out my startup reagents and protocols. I am seeing a lot of protocols for the Technovit system, which uses a slide press to adhere the sections to the slides, and would prefer to avoid expensive startup equipment if possible. I found another protocol that uses silanized slides, water and heat to adhere the sections. Is this comparable to results seen with a slide press, or is there some obvious down-side? I have been mainly looking into MMA/PMMA protocols for my bone, and ideally would like to be able to use the sections for antibody stains in addition to histology stains and mineral assessment- although I will work with the limitations of the medium, I know I can’t always ask for the moon. I will be using adult mouse bones, primarily from appendicular skeleton (long bones). I am trying to start with a relatively do-it-yourself, low throughput option that minimizes startup cost for a system that I may only use short-term. Up until now I have been using either paraffin embedding (decalcified samples), or frozen Cryojane sections (unfixed, undecalcified), but there is potential for plastic to be the best option in some instances. I think I have the sectioning capabilities covered, but would be appreciative of embedding and sectioning protocols (and sage advice from the wise, experienced bone cutters out there, if I’m totally headed in the wrong direction!). A catalog# recommendation for molds/chucks/cassettes (to fit or otherwise adapt to a Leica RM2255 microtome) would be fantastic. Thanks in advance for your help and support. Sincerely, Nicole Collette Lawrence Livermore National Lab collet...@llnl.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Could you please recommend? Cryostats.
Hi, I am also in research, and as such I am at the bottom of the barrel when it comes to NEW equipment. (Yes even lower than normal histo labs, think on that!) I congratulate you on being able to wrangle new equipment out of management. Due to this I can't recommend specific equipment, but I can recommend specific features. I have used cryostats clinically as well as in research, and one thing I noticed absent from most cryostats is the ability to adjust your block. For the life of me I cannot conceive of a reason why a company would have the audacity to sell a cryostat that does not allow the block to tilt. If I was in the market for a cryostat, I would not even consider one without this feature. Good Hunting, Amos On Wed, Jun 29, 2011 at 1:02 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 8 Date: Wed, 29 Jun 2011 12:57:05 -0400 From: Bascaramurty, Saro saro.bascaramu...@nrc-cnrc.gc.ca Subject: [Histonet] Could you please recommend? To: 'Histonet' histonet@lists.utsouthwestern.edu Message-ID: af07ba1afa000448a60fb8ebaae338e7a1524...@nrccenmb2.nrc.ca Content-Type: text/plain; charset=us-ascii Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your positive and negative experiences with the models that you own. Which ones we should stay away from and which ones that you did not regret that you purchased it. Also some pricing details would be helpful. There were some earlier postings on this topic earlier which I did not bother to save. Thank you all in advance for your anticipated feedback. Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramu...@nrc-cnrc.gc.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Could you please recommend?
I have in my lab crytome FSE from shadon and it is working pretty well since last 5 years without any complain. Though i haven't experienced personally, but i heard this is better than leica one. also it has fumigation feature. Amita From: Bascaramurty, Saro saro.bascaramu...@nrc-cnrc.gc.ca To: 'Histonet' histonet@lists.utsouthwestern.edu Date: 29/06/11 10:31 PM Subject:[Histonet] Could you please recommend? Sent by:histonet-boun...@lists.utsouthwestern.edu Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your positive and negative experiences with the models that you own. Which ones we should stay away from and which ones that you did not regret that you purchased it. Also some pricing details would be helpful. There were some earlier postings on this topic earlier which I did not bother to save. Thank you all in advance for your anticipated feedback. Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramu...@nrc-cnrc.gc.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
