[Histonet] Re:
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[Histonet] ki67/mart 1 double stain
any sugestion on validating ki67/mart 1 on the Ventana XT or Ultra. I need a protocol to start. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Wax Waste Disposal
I always incinerated my used paraffin René J --- On Fri, 7/22/11, Joanne Clark jcl...@pcnm.com wrote: From: Joanne Clark jcl...@pcnm.com Subject: [Histonet] Paraffin Wax Waste Disposal To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, July 22, 2011, 4:22 PM Hi All, we had our CAP inspection yesterday and were cited for disposing of our waste wax from the processors in regular waste. In all my 20+ years of working in histology I have never disposed of the dirty wax in biohazard waste. Especially now with the newer processors that have very little carry over. I know this is probably state regulated by is anyone aware of a regulation or documentation that states what the amount of hazardous chemical in a substance must be before it is considered hazardous? And if so, does anyone know of a way to measure the amount of xylene in waste paraffin? Thanks in advance. Joanne Clark, HT Histology Supervisor PCNM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recycled alcohol
Message: 9 Date: Fri, 22 Jul 2011 07:06:19 -0400 From: histot...@imagesbyhopper.com histot...@imagesbyhopper.com Subject: [Histonet] Recycled alcohol To: Histonet@Lists. Utsouthwestern. Edu histonet@lists.utsouthwestern.edu Message-ID: a7b6c430-34ed-4666-86e5-8d2fa304f...@imagesbyhopper.com Content-Type: text/plain; charset=us-ascii Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic HE stainers? Are you having any troubles with proper, consistent HE staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene. After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this! Can anyone help me? Thanks! Michelle We test and use our recycled alcohol as 95% alcohol - we do not sub it for the 100%. We also use recycled xylene with no issues. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Tech seeks job in Florida
Received initial training in Denver, Co. and have been employed in Tallahassee for the past 6 yrs. Ideal position would ibe a firs t sh ift position with general bench work. Strong grosser if needed. My areas of interests are the east coast ( Jacksonville down to New Smyrna Beach ) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] ki67/mart 1 double stain
anyone using ki67/mart 1 double stain have a protocol to share on histonet? - Forwarded Message - From: Dorothy Glass techman...@yahoo.com To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Saturday, July 23, 2011 9:31 AM Subject: [Histonet] ki67/mart 1 double stain any sugestion on validating ki67/mart 1 on the Ventana XT or Ultra. I need a protocol to start. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
