[Histonet] picture of gross lab senior
No instructions sent to install a thin monitor/keyboard bracket on a gross lab senior.Can anyone send me a photo so we can match it up. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] picture of gross lab senior
gross lab senior = obese elderly technician? i don't think s/he would appreciate you installing a keyboard on said person. emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 9, 2011 at 9:10 AM, Vickroy, Jim vickroy@mhsil.com wrote: No instructions sent to install a thin monitor/keyboard bracket on a gross lab senior.Can anyone send me a photo so we can match it up. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing tech openings in Westchester Cty NY
Please contact me today for immediate consideration. Both day and night shift available. If you might know anyone who would be interested, that would be great as well! Thanks! POSITION SUMMARY Perform duties that relate to the histology specimen grossing of surgically removed tissue under the supervision of the Pathology Assistant / Pathologist and/or laboratory supervisor. ESSENTIAL FUNCTIONS * Ensure specimen integrity upon arrival and through out the entire process. * Dictate a gross description for each biopsy specimen, dissect and prepare tissue for cassettes. * Gross core needle biopsy and all anatomical pathology specimens (under the supervision of a Pathologist Assistant and /or pathologist). * Prepare dissected tissue specimens and place into cassettes. * Load prepared cassettes in Tissue Processors. * Operation of all tissue processors. * Familiar with safety procedures and standard operating procedures. * Compliance with all Federal and State regulations pertaining to Grossing: * Adhere to workload requirements. * Maintenance of accurate work records (i.e., Maintenance Logs, Filter Logs, Bench Excerpts etc.). * Able to evaluate new laboratory equipment and procedures. * Able to perform Quality Control and Quality Assurance Procedures. * Working knowledge and understanding of TPS / Lab Manager IS System. * Assist in the professional development of laboratory aides. * Daily housekeeping. POSITION REQUIREMENTS . Associated Degree in Biology and Chemistry; or successful completion of a NAACLS Accredited Histotechnology Program; or Associate Degree with at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university with a combination of 12 semester hours (18 quarter hours) of biology and chemistry. Must be NY State License for CLT or HT. . 3-4 Years as a grossing technician * Must be able to work with hazardous chemicals. DESIRED * Knowledgeable in the handling of specimens from accessioning to final specimen storage. * Working knowledge of laboratory organization, operational procedures, Quality Control, Quality Assurance, and laboratory safety. * Working knowledge and understanding of computers. * Cooperative working relationships with peers and management. * Dependable, flexible and adaptable in all aspects of work. * * * * * * * * * * Brian Feldman * Principal * Prometheus Healthcare * Office 301-693-9057 * Fax 301-368-2478 * br...@prometheushealthcare.com mailto:br...@prometheushealthcare.com * www.prometheushealthcare.com http://www.prometheushealthcare.com/ http://www.prometheushealthcare.com/ * *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** *http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Medite Equipment Histo Pro 3030 Robotic Slide Stainer
Please note that HistoPro® 3030 Robotic Slide Stainer is designed and manufacture in USA by RUSHABH Instruments in Pennsylvania. You can contact RUSHABH Instruments at 215-491-0081 for support. Thanks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Monday, August 08, 2011 9:19 PM To: Histonet Subject: [Histonet] Medite Equipment Does anyone have an opinion good or bad on Medite Equipment? Specifically a TPC 15 Tissue Processor or Histo Pro 3030 Robotic Slide Stainer. And if so, who does the servicing on the equipment. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1391 / Virus Database: 1520/3822 - Release Date: 08/08/11 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening KC, Missouri
Busy Derm practice looking for an independant, hard working histotech, for a dermatopathology lab. Must be proficient in all aspect of routine histology. Grossing, staining, accessioning, mircotonomy. Full time position. Please call Tammi for immediate consideration. (816)584-8100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] position opening
Due to an upcoming retirement, PhenoPath has a position opening in the contract research department. This position is as described below. I'm happy to answer any questions on this position. Contract Research Laboratory Supervisor PhenoPath Laboratories, PLLC, has an opportunity for a Laboratory Supervisor in its Contract Research Department. This is a supervisory position located in Seattle, WA and reports to the Director of Contract Research. Primary Responsibilities Responsibilities include, and are not limited to: Project Development – managing all aspects of client requests for RD projects to include project proposals, reporting project status, and resolution of questions; Project Execution – oversee and coordinate work of Research Technologists to include assay development, specimen tracking and testing, and quality review of slides; Supervision of Contract Research personnel – staff training and development, performance management, assist in recruiting efforts for new staff. Required Skills/Experience Requires 8-10 years of Immunohistochemistry experience and 3-5 years of supervisory and project management experience; preference for an HTL or an MT with experience working in a clinical laboratory or GLP laboratory setting. TO APPLY, PLEASE SEND COVER LETTER AND RESUME TO: PhenoPath Laboratories, PLLC 551 N. 34th St., Suite 100 Seattle, WA 98103 Phone: 206 374-9000 Fax: 206 374-9009 E-mail: j...@phenopath.com Patti Loykasek PhenoPath Laboratories Clinical Lab Manager Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Knife for trimming paraffin
I currently use a paratrimmer. However, in the past I used an extremely dulled metal scalpel with a non-disposable scalpel. I dulled it by scraping it on concrete then on cardboard boxes. It is perfect for popping blocks out of the molds and also the perfect size for quickly scraping the sides of the blocks. I find that it is a lot easier and safer to use than the paring knife that many histotechs use. Carrie L Dula, HTL, SLS(ASCP) --- On Tue, 8/9/11, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: From: histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 11 To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 9, 2011, 10:25 AM Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Re: Knife for trimming paraffin from blocks (histot...@imagesbyhopper.com) 2. RE: Histonet Digest, Vol 93, Issue 9 (Joanne Clark) 3. : [Histonet] re-cycled xylene in tissue processor (White, Lisa M.) -- Message: 1 Date: Mon, 08 Aug 2011 11:46:55 -0400 From: histot...@imagesbyhopper.com histot...@imagesbyhopper.com Subject: Re: [Histonet] Re: Knife for trimming paraffin from blocks To: John Shelley jshel...@sanfordburnham.org Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Cormier, Kathleen kathleen.corm...@crl.com Message-ID: 7ac5a3c6-0aad-434a-962d-5f44b523e...@imagesbyhopper.com Content-Type: text/plain; charset=us-ascii I agree, the Para-Trimmer is the next best thing since sliced bread! I'll never go back to the knife again. We use lid from one of the Ventana special stains boxes to catch the wax. I have also fashioned a drip tray out of the cardboard boxes that the coverslips come in. We're all McGuyver's at heart, aren't we? :o) Sent from my iPhone On Aug 8, 2011, at 11:14 AM, John Shelley jshel...@sanfordburnham.org wrote: Hi All, I likewise use the Para-trimmer and instead of a specimen cup I just use the top lid of a 1/2 gross microscope slide box. Once filled I just throw away in the trash. Kind Regards! John J Shelley Senior Research Associate, Histology Core -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Monday, August 08, 2011 11:04 AM To: gayle callis; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks We use the paratrimmer too. What we do for a paraffin catcher is to use a specimen cup (I call them urine cups, but whatever) to catch the paraffin. When it get fullish, we place in the slide oven to melt the paraffin, when melted pour off, and reuse the cup... :) Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.corm...@crl.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. * Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer
[Histonet] Old Green Staining Racks
Does anyone remember the old green staining racks that had an inverted L metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old Green Staining Racks
Market Lab cat#ML1212. I would check the cat# before ordering, the picture in the catalog show the right rack but the description is different. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Tuesday, August 09, 2011 11:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Green Staining Racks Does anyone remember the old green staining racks that had an inverted L metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Napsin-A
I am starting to work up Napsin-A (purchased from Biocare Medical) on our Ventana instruments using iView DAB. Does anyone have a recommended protocol to get me started? Amanda Steier, HT(ASCP) Sanford Health Fargo, ND --- Please note that My Email Address Has Changed! Please begin using the address in the From line above, immediately. Soon, email sent to my old address will no longer be delivered to me. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TFE-3
Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: TFE-3
We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Old Green Staining Racks
Mercedes Medical MER1040 - Slide Staining Dipper, with metal handle - took me awhile to find them too. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Tuesday, August 09, 2011 9:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Green Staining Racks Does anyone remember the old green staining racks that had an inverted L metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: TFE-3
Same here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, August 09, 2011 11:05 AM To: 'Houston, Ronald'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: TFE-3 We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Manual embedding
Hi, All, I just saw this question and the responses, thought I would add my own solution to the mix. I do manual embedding, I use a hyb oven for my infiltrating/embedding station (like for hybridizing Southern or Northern blots- who does that anymore?). I have taken out the rotating wheel for spinning bottles, and line the tray at the bottom with foil. Temp control works very well. I have several Wheaton staining boxes (the kind that come with glass inserts for staining 20 slides) that I use for my wax changes for infiltrating, and a couple of metal beakers I use for pouring into molds. I have a little real estate left inside the oven (it's probably around 18x 18 square area) to heat my molds for pouring, and I have a metal heat block in there that you would use for Eppendorf tubes as my forceps warmer. It works pretty well, but does take a long time to heat up those boxes of wax, so I need to plan ahead by about 3 hours. When I pour the molds, I have the oven in front of a drawer under the bench, I line an extra cabinet shelf with foil and lay it over the open drawer to give me some bench space, pour my molds inside the oven and transfer it to the foil-lined shelf to cool so I can move it without the specimen shifting. Then I transfer to a photo tray and cool in the fridge for a couple of hours before releasing the molds. With the door of the hyb oven open I have to embed in shifts and let the molds heat up again, but it works OK. At least it's all contained in one unit. It's hard to do histology in a molecular lab ;) Hope this helps to give a do-it-your-selfer some ideas. Sincerely, Nicole Collette LLNL On 8/2/11 7:53 PM, Scott Parker spar...@vt.edu wrote: Dear Histonetters: I am interested in acquiring a pitcher and heating jacket for melting and pouring paraffin during manual embedding. My work is relatively low volume and in a university research lab setting so I am trying to avoid purchasing an expensive embedding station. Can anyone recommend an honest supplier of used histology equipment that might be able to provide me with this item? Thank you for your expertise! Scott L. Parker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What's your favorite phospho-histone H3 antibody for IHC?
p(Ser10) or p(Ser28) ? And have you found pHH3 (Ser28) to be more M-phase specific? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fern tissue loss during in situ hybridization
Dear Histonet members, I’m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I’m not an expert at this process, but I’ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt’s coated slies), and I’ve had success with Arabidpsis ISH. I’ll outline the relevant and troubleshooting procedures that I’ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37°C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I’ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48°C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem!--Angie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fern tissue loss during in situ hybridization
I think you should try to find out about the cellulose contents of the fern you are working with, and compare it to that of Arabidopsis. If the fern (Ceratopteris) contains more cellulose, I think this is the cause of your problem. If that is the case I think you should try to use a paraffin wax of higher melting point (63-65ºC) to assure an infiltration more compatible with the cellulose. Also make sure that the infiltration is optimal. Finally if you can use thinner sections (7 µm) that could help also. René J. --- On Tue, 8/9/11, Cordle, Angela R angela-cor...@uiowa.edu wrote: From: Cordle, Angela R angela-cor...@uiowa.edu Subject: [Histonet] Fern tissue loss during in situ hybridization To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, August 9, 2011, 4:46 PM Dear Histonet members, I’m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I’m not an expert at this process, but I’ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt’s coated slies), and I’ve had success with Arabidpsis ISH. I’ll outline the relevant and troubleshooting procedures that I’ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37°C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I’ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48°C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem! --Angie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sterilized cassette
My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking out of the box on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
