[Histonet] heavy duty microtome

2011-08-26 Thread Jette Barlach
Does anyone have either a used Leica Polycut E  or SM 2500 for sale.
For the present we are using a very old (50 years) Reichert-Jung heavy duty 
microtome and looking for a replacement.

If you can help us please contact:

Jette Barlach
Research unit for Rheumatology an Bone biology
Dept. of Rheumatology
University hospital of Aarhus
Nørrebrogade 44
8000 Aarhus C
Denmark
j...@ana.au.dkmailto:j...@ana.au.dk
+45 89 42 29 92

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[Histonet] (no subject)

2011-08-26 Thread mohamed abd el razik
a href=http://hekkles.com/vffws.htm;http://hekkles.com/vffws.htm/a
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RE: [Histonet] RE: Embedding process improvementandcompetencyassessment

2011-08-26 Thread Heath, Nancy L.
If people cheated. Then people cheated. There will always be that
element. To discard that requirement (the practical) because of the
cheat factor was a silly (remember, this is my rantnot yours) excuse
that I have heard from some people who were part of the decision.  What
was lost was the incredable effort that was needed to do that practical
and the experience of being able to produce registry quality slides.
That is not cheating themselves, but robbing every pathologist and
patient that followed until that person started to produce quality
work.

I could not agree more with what Bill O'Donnell wrote!! Thank you Bill.
I have students in the clinical portion of there histo training and
though I am not former military my students sure do think I am!! I
strive for perfection from the time they section in their first piece of
tissue till their last stained slide. 

Nancy Heath, HT(ASCP)

-Original Message-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net] 
Sent: Thursday, August 25, 2011 4:48 PM
To: Podawiltz, Thomas; Heath, Nancy L.; Jennifer MacDonald
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process
improvementandcompetencyassessment

Tom, (and all those following this thread)

And we got that ;) perfection. 

(Warning, long rant ahead... Rene's might have been longer, but he
spread it out over a number of posts)

(Hi Rene, I enjoyed your rant.)

My biggest barrier in assimilating into the civilian workforce was
that perfection wasn't required or even at times expected. I still can't
get my head around that. Because of what was expected of me by my unit,
30 years later, my embedding is tight w nice, neat rows, cutting is neat
and aligned and staining is crisp. (However, I will not win any speed
contest) My point in this is not to blow my horn. Most of you out there
turn out the same or better quality. My point is this, when supervisors
and peers stop striving for perfection then there will always be a need
for skills assessments and re-training. There will be little or no
perfection.


Over the last thirty years of training and supervising, I have always
tried to instill the idea that we are dealing with a person's tissue.
Wrapped up in that is a lot of anxiety and stress that the patient is
having. The first thing a new trainee or tech had to do when they came
to me (in the civilian world)was spend a week shadowing phlebotomy so
that they got the chance to see some of the faces behind the samples. 

But in the last ten years or so, there has been too much emphasis on
speed as a standard of performance, and in general, there has been a
growing attitude that perfection isn't possible. (many factors to blame,
I suppose)

I once worked for a very demanding pathologist and because of his
expectations, the whole crew put out near perfection. Pathologists that
are OK with what they get, so long as they can make a diagnosis, are a
huge part of that problem.

But as supervisors, we only get what we expect of people and strive for
and example ourselves. 

(The rest of the rant is anecdotal and not very interesting, but since I
took the time to write it, here it is...)

This was also the period (Navy)when I had to do that monster practical
for the HT. Back then, it was not 7 or 8 slides, but many more with a
large number of special stains as well. I knew if it passed the guys in
our lab, it should be no problem passing the practical. The guy who took
the test at the same time I did, picked his tissues on Wednesday,
embedded them on Thursday cut cut and stained them on Saturday and
mailed them on Monday. He had no concern at all that his work wouldn't
be good enough. He passed the practical with a very respectable
percentile. I think that helps to bolster the idea that an expectation
that is demanded and fostered is one that can be confidently met. 

If people cheated. Then people cheated. There will always be that
element. To discard that requirement (the practical) because of the
cheat factor was a silly (remember, this is my rantnot yours) excuse
that I have heard from some people who were part of the decision.  What
was lost was the incredable effort that was needed to do that practical
and the experience of being able to produce registry quality slides.
That is not cheating themselves, but robbing every pathologist and
patient that followed until that person started to produce quality work.

(Rany over, thanks to those who hung out til the end I hope it was
worth it)

I hope every one has a great weekend, as I am off on Friday and will be
having a great weekend myself. Shalom - Bill

-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Thursday, August 25, 2011 1:21 PM
To: O'Donnell, Bill; Heath, Nancy L.; Jennifer MacDonald
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley
Subject: RE: [Histonet] RE: 

RE: [Histonet] heavy duty microtome

2011-08-26 Thread Marcum, Pamela A
I have used the Leica RM2255 and  RM 2155s for paraffin and non-decalcified 
bone in MMA and they are work horses.  
Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van 
Meter
Sent: Friday, August 26, 2011 7:20 AM
To: Jette Barlach
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] heavy duty microtome

Contact Leica to see if they have any demo models for sale.



Sent from my iPhone

On Aug 26, 2011, at 2:10 AM, Jette Barlach j...@ana.au.dk wrote:

 Does anyone have either a used Leica Polycut E  or SM 2500 for sale.
 For the present we are using a very old (50 years) Reichert-Jung heavy duty 
 microtome and looking for a replacement.
 
 If you can help us please contact:
 
 Jette Barlach
 Research unit for Rheumatology an Bone biology Dept. of Rheumatology 
 University hospital of Aarhus Nørrebrogade 44 8000 Aarhus C Denmark 
 j...@ana.au.dkmailto:j...@ana.au.dk
 +45 89 42 29 92
 
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Re: [Histonet] AMPHYL

2011-08-26 Thread Pam Marcum

We can't find Lysol IC here either so any supplier would help and to keep costs 
down a store would be better than a scientific supplier it possible.  Please id 
anyone knows let us all know as it is not easy to find. 



Pam Marcum 










- Original Message -

From: Rae Staskiewicz raest...@grics.net 
To: Sebree Linda A lseb...@uwhealth.org, Barbara Crill 
barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu 
Sent: Thursday, August 25, 2011 4:53:36 PM 
Subject: RE: [Histonet] AMPHYL 

Linda, 

Where do you get your Lysol IC? 

Rae Staskiewicz 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda 
A 
Sent: Thursday, August 25, 2011 1:07 PM 
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] AMPHYL 

We use Lysol I. C.; haven't be able to get Amphyl for years. 


Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
barbara.cr...@lpnt.net 
Sent: Thursday, August 25, 2011 12:56 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] AMPHYL 

My materials management department told me that AMPHYL has been 
discontinued. 
Ventana recommends that we clean/decontaminate the Benchmark and the 
Ultras with AMPHYL. 

Has anyone else ran across this?  Has AMPHYL really been discontinued? 
Is there a substitute we can use. 

Thanks everyone! 


ANTOINETTE CRILL 
ANATOMIC PATHOLOGY 
EXT 5451 

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RE: [Histonet] AMPHYL

2011-08-26 Thread Bouchal, Rena L
After Amphyl was discontinued, we switched to Steris Lph Se. Here are the 
comparisons of the two:
Both are germicidal, fungicidal, and virucidal; both contain active ingredients 
0-phenylophenol (amphyl at 2.80%and Steris at 7.7%) instead of the other active 
ingredient in amphyl (0-benzyl-p-chlorophenol at 7.2%) Steris contains 
p-tertiary-amphylphenol at 7.6%.  Amphyl is diluted 40cc/1gal for a 1:100 ratio 
and Steris is diluted 30cc/1gal for 1:100 ratio. The order number for the 
Steris LpH Se is 6466-08.  Hope this helps.

Please note that my email address  as of Jan 3, 2011 is 
bouch...@wvuhealthcare.com  .   Please make the appropriate changes in your 
address book.

Rena Bouchal, M.S.
Anatomic Pathology Manager
West Virginia University Hospitals
304-293-7765

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Friday, August 26, 2011 9:39 AM
To: Rae Staskiewicz
Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A
Subject: Re: [Histonet] AMPHYL


We can't find Lysol IC here either so any supplier would help and to keep costs 
down a store would be better than a scientific supplier it possible.  Please id 
anyone knows let us all know as it is not easy to find. 



Pam Marcum 










- Original Message -

From: Rae Staskiewicz raest...@grics.net 
To: Sebree Linda A lseb...@uwhealth.org, Barbara Crill 
barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu 
Sent: Thursday, August 25, 2011 4:53:36 PM 
Subject: RE: [Histonet] AMPHYL 

Linda, 

Where do you get your Lysol IC? 

Rae Staskiewicz 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda 
A 
Sent: Thursday, August 25, 2011 1:07 PM 
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] AMPHYL 

We use Lysol I. C.; haven't be able to get Amphyl for years. 


Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
barbara.cr...@lpnt.net 
Sent: Thursday, August 25, 2011 12:56 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] AMPHYL 

My materials management department told me that AMPHYL has been 
discontinued. 
Ventana recommends that we clean/decontaminate the Benchmark and the 
Ultras with AMPHYL. 

Has anyone else ran across this?  Has AMPHYL really been discontinued? 
Is there a substitute we can use. 

Thanks everyone! 


ANTOINETTE CRILL 
ANATOMIC PATHOLOGY 
EXT 5451 

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[Histonet] Embedding Center Questions

2011-08-26 Thread Tim Wheelock

Hi All:

My 23 year old Shandon Embedding Center may be ready to give up the ghost.
It has had two repairs of the coolant system for the freezing plate over 
the last year.
Now a thermo-resistor has burnt out, so that the wax reservoir with no 
long melt the wax.


Does anyone know of a company or companies that lease/rent histology 
equipment?

Are there companies who sell used equipment?
Also, what do people recommend as far as a new embedding center, in case 
we can afford it?

I am in the Boston area.

In the meantime, maybe I can jury-rig something, since it is only the 
wax reservoir that is down.
The cassette holding tank, hot plate, and freezing plate are OK for the 
moment.
Maybe I can melt a few jars of wax, and use a transfer pipet to fill the 
molds?

Any ideas?

Thanks,

Tim


Tim Wheelock
Assistant Director, Neuropathology
Instructor In Neuroanatomy
Harvard Brain Tissue Resource Center
203 Mailman Research Center
McLean Hospital
Belmont MA 02478
Phone: 617-855-3592
Fax: 617-855-3199

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[Histonet] Embedding competency and med techs running a Histo lab

2011-08-26 Thread D'Attilio, Shelley
Hi all,
Thanks so much for the advice I received following my request for help on our 
embedding competency improvement project.  I enjoyed the lively discussion that 
followed about non-histotechs managing a Histology lab and I appreciated all 
the kind comments and words of encouragement both on and off the listserv 
board.  

Regards,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas

 





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accepting new patients.  Call (785) 354-5225.

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RE: [Histonet] AMPHYL

2011-08-26 Thread Sebree Linda A
We've been getting our Lysol IC  from Cardinal Health but in the past
we've had to go through Amazon.com
 

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 

 



From: Pam Marcum [mailto:mucra...@comcast.net] 
Sent: Friday, August 26, 2011 8:39 AM
To: Rae Staskiewicz
Cc: Sebree Linda A; Barbara Crill; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] AMPHYL


We can't find Lysol IC here either so any supplier would help and to
keep costs down a store would be better than a scientific supplier it
possible.  Please id anyone knows let us all know as it is not easy to
find.

 

Pam Marcum








From: Rae Staskiewicz raest...@grics.net
To: Sebree Linda A lseb...@uwhealth.org, Barbara Crill
barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu
Sent: Thursday, August 25, 2011 4:53:36 PM
Subject: RE: [Histonet] AMPHYL

Linda, 

Where do you get your Lysol IC?

Rae Staskiewicz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree
Linda
A
Sent: Thursday, August 25, 2011 1:07 PM
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] AMPHYL

We use Lysol I. C.; haven't be able to get Amphyl for years. 


Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
barbara.cr...@lpnt.net
Sent: Thursday, August 25, 2011 12:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AMPHYL

My materials management department told me that AMPHYL has been
discontinued.
Ventana recommends that we clean/decontaminate the Benchmark and the
Ultras with AMPHYL.

Has anyone else ran across this?  Has AMPHYL really been discontinued?
Is there a substitute we can use.

Thanks everyone!


ANTOINETTE CRILL
ANATOMIC PATHOLOGY
EXT 5451

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RE: [Histonet] Embedding Center Questions

2011-08-26 Thread Rathborne, Toni
A couple of places that come to mind are Belair (800-783-9424), Medical 
Equipment Source (www.medequipsource.com), and  Mayflower (800-287-9801). I 
know that they sell used equipment, you could inquire about a rental.
Good luck.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Friday, August 26, 2011 9:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding Center Questions

Hi All:

My 23 year old Shandon Embedding Center may be ready to give up the ghost.
It has had two repairs of the coolant system for the freezing plate over the 
last year.
Now a thermo-resistor has burnt out, so that the wax reservoir with no long 
melt the wax.

Does anyone know of a company or companies that lease/rent histology equipment?
Are there companies who sell used equipment?
Also, what do people recommend as far as a new embedding center, in case we can 
afford it?
I am in the Boston area.

In the meantime, maybe I can jury-rig something, since it is only the wax 
reservoir that is down.
The cassette holding tank, hot plate, and freezing plate are OK for the moment.
Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds?
Any ideas?

Thanks,

Tim


Tim Wheelock
Assistant Director, Neuropathology
Instructor In Neuroanatomy
Harvard Brain Tissue Resource Center
203 Mailman Research Center
McLean Hospital
Belmont MA 02478
Phone: 617-855-3592
Fax: 617-855-3199

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[Histonet] Jill's MSDS question: a tech's perspective

2011-08-26 Thread Cheryl
Hi Jill-- Congrats on the job!
 
The MSDS in paper vs. eCopy copy will come down to the opinion of your 
inspector.  Like Wil says, he's passed before...and I've passed before but only 
because we had both eCopies and hard copies.  Most of the larger for-profit and 
major health system administrations require both by SOP regardless, for 
liability reasons usually but please read on!
 
Better yet, think of the practical uses and real-world applications 
of this rather than the 'do it because I have to' ones:
 
If you or one of yours gets splashed with something--how do you want the next 
response to go?  If you only have eCopies and the power is down, or you are 
having computer issues, or the PC is slow, or the printer on the network isn't 
responding, then what? If it's your eyes or your skin that's about to be 
irreversably burnt, wouldn't you want that information available in a TOTALLY 
reliable manner and a copy available to grab, follow AND send to the emergency 
room with you--RIGHT NOW?  Then add to it the liability for your company should 
this be the case...and we all know when you most need your PC to work is when 
it doesn't.
 
If I'm having trouble making a decision often exaggerating the potential 
circumstances makes the answer far easier to come to.
 
If I am working in a lab I want hard copies alphabetized by both the mfc. 
chemical name and the common name we use for it in the lab with an index with 
the binders (Print the covers black on yellow paper and insert these in clear 
view binders) on the benches in the space where we work.  Not on a computer or 
off in the transcription area.  It may not be required by your inspector or by 
your facility, but is that really why we have these in our labs in the first 
place--because we're required to do so?  
 
My four cents
 
Cheryl
 
Cheryl Kerry, HT(ASCP)
Full Staff Inc - Staffing the AP Lab one GREAT tech at a time.
281.852.9457
ad...@fullstaff.org
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RE: [Histonet] heavy duty microtome

2011-08-26 Thread Jack Ratliff

I know of two possible options for exactly what you seek. Please contact me 
directly so that we may point you in the right direction and discuss with you 
the details.
 
Best Regards,
 
 
Jack
 
PS I have heard a rumor that these units will no longer be for sale by Leica 
and no replacements will be available in the future. Additionally, I very 
seriously doubt that they have a demo unit for sale as they typically 
manufacture these units upon approved purchase order.
 
 
Jack L Ratliff
Senior Histologist, BioMimetic Therapeutics, Inc.
Chair, Hard Tissue Committee - National Society for Histotechnology
 

 

 From: j...@ana.au.dk
 To: histonet@lists.utsouthwestern.edu
 Date: Fri, 26 Aug 2011 09:08:29 +0200
 Subject: [Histonet] heavy duty microtome
 
 Does anyone have either a used Leica Polycut E or SM 2500 for sale.
 For the present we are using a very old (50 years) Reichert-Jung heavy duty 
 microtome and looking for a replacement.
 
 If you can help us please contact:
 
 Jette Barlach
 Research unit for Rheumatology an Bone biology
 Dept. of Rheumatology
 University hospital of Aarhus
 Nørrebrogade 44
 8000 Aarhus C
 Denmark
 j...@ana.au.dkmailto:j...@ana.au.dk
 +45 89 42 29 92
 
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RE: [Histonet] AMPHYL

2011-08-26 Thread McMahon, Loralee A
http://www.labsafety.com/lysol-ic-quaternary-cleaner-disinfectant_24530427/


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum 
[mucra...@comcast.net]
Sent: Friday, August 26, 2011 9:39 AM
To: Rae Staskiewicz
Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A
Subject: Re: [Histonet] AMPHYL

We can't find Lysol IC here either so any supplier would help and to keep costs 
down a store would be better than a scientific supplier it possible.  Please id 
anyone knows let us all know as it is not easy to find.



Pam Marcum










- Original Message -

From: Rae Staskiewicz raest...@grics.net
To: Sebree Linda A lseb...@uwhealth.org, Barbara Crill 
barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu
Sent: Thursday, August 25, 2011 4:53:36 PM
Subject: RE: [Histonet] AMPHYL

Linda,

Where do you get your Lysol IC?

Rae Staskiewicz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Thursday, August 25, 2011 1:07 PM
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] AMPHYL

We use Lysol I. C.; haven't be able to get Amphyl for years.


Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
barbara.cr...@lpnt.net
Sent: Thursday, August 25, 2011 12:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AMPHYL

My materials management department told me that AMPHYL has been
discontinued.
Ventana recommends that we clean/decontaminate the Benchmark and the
Ultras with AMPHYL.

Has anyone else ran across this?  Has AMPHYL really been discontinued?
Is there a substitute we can use.

Thanks everyone!


ANTOINETTE CRILL
ANATOMIC PATHOLOGY
EXT 5451

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[Histonet] AUTO: Gina Rodriguez is out of the office. (returning 09/06/2011)

2011-08-26 Thread Gina . Rodriguez

I am out of the office until 09/06/2011.

I will respond to your message when I return.


Note: This is an automated response to your message  Histonet Digest, Vol
93, Issue 35 sent on 8/26/2011 8:45:07 AM.

This is the only notification you will receive while this person is away.


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RE: [Histonet] AMPHYL

2011-08-26 Thread Shirley A. Powell
Google Lysol IC and you will get plenty of suppliers.
sp

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Friday, August 26, 2011 9:39 AM
To: Rae Staskiewicz
Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A
Subject: Re: [Histonet] AMPHYL


We can't find Lysol IC here either so any supplier would help and to keep costs 
down a store would be better than a scientific supplier it possible.  Please id 
anyone knows let us all know as it is not easy to find. 



Pam Marcum 










- Original Message -

From: Rae Staskiewicz raest...@grics.net 
To: Sebree Linda A lseb...@uwhealth.org, Barbara Crill 
barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu 
Sent: Thursday, August 25, 2011 4:53:36 PM 
Subject: RE: [Histonet] AMPHYL 

Linda, 

Where do you get your Lysol IC? 

Rae Staskiewicz 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda 
A 
Sent: Thursday, August 25, 2011 1:07 PM 
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] AMPHYL 

We use Lysol I. C.; haven't be able to get Amphyl for years. 


Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
barbara.cr...@lpnt.net 
Sent: Thursday, August 25, 2011 12:56 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] AMPHYL 

My materials management department told me that AMPHYL has been 
discontinued. 
Ventana recommends that we clean/decontaminate the Benchmark and the 
Ultras with AMPHYL. 

Has anyone else ran across this?  Has AMPHYL really been discontinued? 
Is there a substitute we can use. 

Thanks everyone! 


ANTOINETTE CRILL 
ANATOMIC PATHOLOGY 
EXT 5451 

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[Histonet] OT: Hurricane Irene

2011-08-26 Thread Pam Barker
Hi Histonetters!
Are you in the projected path of Irene?  I'm a long time florida
resident who has weathered hurricanesfalse alarms in both central
Floridathe panhandle. It's not the hurricane it's the aftermath... No
power which means no gasoline no atms no A.C. no lights, no water. At
least fill your car with gas have cash on handfill your bathtub with
water That way you are prepared and have not spent alot of money on
stuff you wouldn't use on the OFF chance that it doesn't affect you. Be
Safe!!
Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

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Re: [Histonet] Jill's MSDS question: a tech's perspective

2011-08-26 Thread Jill Cox
Hi Cheryl,
Thanks! I do have hard copies here, my main question was about the binder 
color. I ended up ordering a MSDS binder from LSS yesterday. I just wanted all 
manuals to match, lol. I haven't had hard copy in years, everywhere I have been 
has only had online and saved to desktop. We did pass all inspections with 
online only. It's great to hear from you!

Jill Cox, HT ASCP

 






 

From: Cheryl tkngfl...@yahoo.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Friday, August 26, 2011 7:17 AM
Subject: [Histonet] Jill's MSDS question: a tech's perspective

Hi Jill-- Congrats on the job!
 
The MSDS in paper vs. eCopy copy will come down to the opinion of your 
inspector.  Like Wil says, he's passed before...and I've passed before but only 
because we had both eCopies and hard copies.  Most of the larger for-profit and 
major health system administrations require both by SOP regardless, for 
liability reasons usually but please read on!
 
Better yet, think of the practical uses and real-world applications 
of this rather than the 'do it because I have to' ones:
 
If you or one of yours gets splashed with something--how do you want the next 
response to go?  If you only have eCopies and the power is down, or you are 
having computer issues, or the PC is slow, or the printer on the network isn't 
responding, then what? If it's your eyes or your skin that's about to be 
irreversably burnt, wouldn't you want that information available in a TOTALLY 
reliable manner and a copy available to grab, follow AND send to the emergency 
room with you--RIGHT NOW?  Then add to it the liability for your company should 
this be the case...and we all know when you most need your PC to work is when 
it doesn't.
 
If I'm having trouble making a decision often exaggerating the potential 
circumstances makes the answer far easier to come to.
 
If I am working in a lab I want hard copies alphabetized by both the mfc. 
chemical name and the common name we use for it in the lab with an index with 
the binders (Print the covers black on yellow paper and insert these in clear 
view binders) on the benches in the space where we work.  Not on a computer or 
off in the transcription area.  It may not be required by your inspector or by 
your facility, but is that really why we have these in our labs in the first 
place--because we're required to do so?  
 
My four cents
 
Cheryl
 
Cheryl Kerry, HT(ASCP)
Full Staff Inc - Staffing the AP Lab one GREAT tech at a time.
281.852.9457
ad...@fullstaff.org
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Re: [Histonet] Jill's MSDS question: a tech's perspective

2011-08-26 Thread Emily Sours
I would respond to this by saying, if I were working with something that
dangerous, I would know ahead of time what to do with it.  I'm not going to
spend any amount of time looking up an MSDS if something is on me RIGHT NOW,
digital or not.  Which is why I think it makes sense to have digital copies,
because you're going to know how to handle hazardous stuff and digital
copies save space and time.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Aug 26, 2011 at 10:17 AM, Cheryl tkngfl...@yahoo.com wrote:

 Hi Jill-- Congrats on the job!

 The MSDS in paper vs. eCopy copy will come down to the opinion of your
 inspector.  Like Wil says, he's passed before...and I've passed before but
 only because we had both eCopies and hard copies.  Most of the larger
 for-profit and major health system administrations require both by SOP
 regardless, for liability reasons usually but please read on!

 Better yet, think of the practical uses and real-world applications
 of this rather than the 'do it because I have to' ones:

 If you or one of yours gets splashed with something--how do you want the
 next response to go?  If you only have eCopies and the power is down, or
 you are having computer issues, or the PC is slow, or the printer on the
 network isn't responding, then what? If it's your eyes or your skin that's
 about to be irreversably burnt, wouldn't you want that information available
 in a TOTALLY reliable manner and a copy available to grab, follow AND send
 to the emergency room with you--RIGHT NOW?  Then add to it the liability for
 your company should this be the case...and we all know when you most need
 your PC to work is when it doesn't.

 If I'm having trouble making a decision often exaggerating the potential
 circumstances makes the answer far easier to come to.

 If I am working in a lab I want hard copies alphabetized by both the mfc.
 chemical name and the common name we use for it in the lab with an
 index with the binders (Print the covers black on yellow paper and insert
 these in clear view binders) on the benches in the space where we work.  Not
 on a computer or off in the transcription area.  It may not be required by
 your inspector or by your facility, but is that really why we have these in
 our labs in the first place--because we're required to do so?

 My four cents

 Cheryl

 Cheryl Kerry, HT(ASCP)
 Full Staff Inc - Staffing the AP Lab one GREAT tech at a time.
 281.852.9457
 ad...@fullstaff.org
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[Histonet] Re: Embedding process etc.

2011-08-26 Thread Bob Richmond
Bill O'Donnell observes:

I once worked for a very demanding pathologist and because of his 
expectations, the whole crew put out near perfection. Pathologists that are 
OK with what they get, so long as they can make a diagnosis, are a huge 
part of that problem.

I very much agree, though I've often been guilty as charged. Edwards
Deming - whom of course American MBA's learn to ridicule - was very
much concerned with constant feedback, and constant attention to
fixing little problems before they turn into big problems.

The histotechnologist who never looks at a slide and the pathologist
who is afraid to complain about unsatisfactory work are two sides of
the same problem.

This month's Journal of Histotechnology has an article about quality
assurance of GI biopsies that everyone concerned with this issue
should read. I'll try to get review of it onto Histonet.

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.

2011-08-26 Thread Diane . Tokugawa

I will be out of the office starting  08/25/2011 and will not return until
08/29/2011.

Note:   For Cytology issues, please call Molly  at 8-421-5487,  Eric at
8-421-5405, Barbara at 8-421-5033, or Wanda 8-421-5426   For Histology
issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408
or Wanda at 8-421-5426.
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RE: [Histonet] RE: Embedding process improvement and competencyassessment

2011-08-26 Thread joelle weaver

I recall being told that one reason for the practical discontinuation, was that 
there were not enough well qualified judges for the practical work available at 
the ASCP, mostly populated with MT trained people in these roles? ..not sure if 
that is true.I am not offering that this is valid for a reason, just that this 
was the information I was given when I questioned the wisdom of that decision.  
I do think that if you cheat, this will catch up with you,but unfortunately not 
immediately most of the time. Sadly I think it does serve to dilute the 
perception of, and respect for, those that had put forth the effort to acheive 
their best , and submit their best  slides(closest to perfection) by their own 
efforts. I can see it might be hard to prove cheaters, given the geographic 
area and number of people involved, but anyone who signed off on someone's 
misrepresented work, well they have to live with themselves and whatever the 
potential results to patients, colleagues et al.
Joelle

Joelle Weaver MAOM, BA, (HTL) ASCP
 

 From: joseph-galbra...@uiowa.edu
 To: bdebrosse-se...@isisph.com; jmacdon...@mtsac.edu; nhe...@lifespan.org
 Date: Thu, 25 Aug 2011 18:07:26 +
 Subject: RE: [Histonet] RE: Embedding process improvement and 
 competencyassessment
 CC: histonet@lists.utsouthwestern.edu; 
 histonet-boun...@lists.utsouthwestern.edu; sdatt...@stormontvail.org
 
 How disgusting to hear about cheating. I recall that someone was supposed to 
 sign off as a witness that the applicant had done the work themselves. I 
 spent months acquiring tissue, processing, embedding, cutting and staining a 
 set of blocks and slides and was rewarded with a high score for the effort. 
 It was something I could be proud of. As I recall we had to submit 25 or so 
 slides back then only some of which were graded and the grading was really 
 strict (but did vary with the grader).
 
 Joe Galbraith HTL (and also MT by the way)
 University of Iowa
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea 
 DeBrosse-Serra
 Sent: Thursday, August 25, 2011 12:14 PM
 To: 'Jennifer MacDonald'; Heath, Nancy L.
 Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu; 
 D'Attilio, Shelley
 Subject: RE: [Histonet] RE: Embedding process improvement and 
 competencyassessment
 
 I heard of a lot of cheating as well. People paid others to do the blocks and 
 staining. How good does it do? In the end, these people are cheating 
 themselves. Very sad!
 
 Beatrice DeBrosse-Serra HT(ASCP)QIHC
 Isis Pharmaceuticals
 Antisense Drug Discovery
 1896 Rutherford Road
 Carlsbad, CA 92008
 760-603-2371
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
 MacDonald
 Sent: Thursday, August 25, 2011 7:58 AM
 To: Heath, Nancy L.
 Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu; 
 D'Attilio, Shelley
 Subject: RE: [Histonet] RE: Embedding process improvement and 
 competencyassessment
 
 I fail to see the correlation of a non HT person supervising the Histology
 lab and the lack of a practical exam for HT/HTL staff. One of the issues
 that Shelley brought up was the staff lost or did not develop their
 embedding skills. Submission of a practical exam is not proof of highly
 developed embedding skills. For the HT exam there were 8 blocks that were
 submitted (9 slides). I know of cases where the blocks were not even
 embedded or cut by the applicant.
 
 
 
 
 Heath, Nancy L. nhe...@lifespan.org
 Sent by: histonet-boun...@lists.utsouthwestern.edu
 08/25/2011 07:11 AM
 
 To
 D'Attilio, Shelley sdatt...@stormontvail.org, Podawiltz, Thomas
 tpodawi...@lrgh.org, Histonet Listserv (E-mail)
 histonet@lists.utsouthwestern.edu
 cc
 
 Subject
 RE: [Histonet] RE: Embedding process improvement and competencyassessment
 
 
 
 
 
 
 This is exactly why the powers that be should have NEVER gotten rid of
 the practical portion of the HT/HTL board certification!
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 D'Attilio, Shelley
 Sent: Thursday, August 25, 2011 9:45 AM
 To: Podawiltz, Thomas; Histonet Listserv (E-mail)
 Subject: [Histonet] RE: Embedding process improvement and
 competencyassessment
 
 Hi Tom,
 Thank you for your kind words. I am off the bench almost completely. I
 can work in the gross room in a pinch and my counting skills are
 excellent, so I can always file slides and block if an emergency
 arises:) I occasionally cover a bench in Chemistry as well, but my
 staff is all pretty glad that I mostly stay in my office.
 
 Thanks so much for the embedding information. The main problem we are
 tackling at the moment is tissue orientation. I have written a pretty
 detailed embedding procedure that is being reviewed by the new 

[Histonet] Fwd: Re: Monday deployed changes to worklist processing

2011-08-26 Thread Maureen Griffo

All,

Jeff's email below is a message that they have successfully re-directed 
the ICC recut (automatic HE request) to the IP worklist now.  The 
unstained slides have also been re-directed there as the majority of 
these requests are for IHC and or FISH.  This lets all have the same 
visual around the unstained slides that were ordered and cut.


Both teams need to keep your eyes on this and get feedback to us 
promptly if you see any issues.  Thanks much!  Let me know of your 
questions.


Maureen

 Original Message 
Subject:Re: Monday deployed changes to worklist processing
Date:   Fri, 26 Aug 2011 10:52:59 -0700
From:   Jeff Cochran j...@pathology.washington.edu
To: Maureen Griffo mgri...@u.washington.edu



Yep. You coming here? Me go to your place?

On 8/26/2011 10:41 AM, Maureen Griffo wrote:

 Hi Jeff,

 Do you have a moment to chat about this in ~15 minutes?

 Thanks,

 Maureen

 On 8/25/2011 9:19 AM, Jeff Cochran wrote:

 Maureen,

 Monday morning (while the Bond is being restarted), we'll deploy the
 alterations to the Bond interface that allow:

 1) Non-staining orders to appear on a worklist monitored for the
 Bond. Orders ICC US and ICC Recut will appear on the IP Worksheet
 worklist with no messages directed to the Bond. Worklist modification
 to orders for tests on the Bond will have the same affect as current.
 For example, moving an order from IP Worksheet to another ICC
 worklist will send a cancel-order message to the Bond (common for
 managing research cases). Worklist modification to orders for tests
 not implemented on the Bond will behave correctly (e.g., moving an
 order for FISH to the IP Worksheet worklist will not cause a message
 to be sent to the Bond).

 2) Procedures configured both by worklist (in routing) and through
 declaration of a protocol name for the Bond. The protocol name is
 the Bond's public name for a test. The feature allows multiple LIS
 procedures mapped to a single Bond test, supporting the CAP-directed
 alterations in resulting for breast panel tests.

 As part of the rework to add these new features, significant work has
 been done to increase stability and scalability in the Bond
 interface, and to access more LIS data when auto-generating negative
 control and HE orders.

 Please let everyone know and please ask that they keep their eyes
 open for any problems on Monday. I'll be keeping the day open for any
 problems that may arise and the team should feel free to grab me or,
 preferably, open a high-priority tech request if they need to.

 Thanks,

 Jeff



--
Jeff Cochran
Senior Computer Specialist
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
(206) 598-1616
(206) 598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


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[Histonet] CMV IHC

2011-08-26 Thread McCabe, Sara J.
Happy Friday Histonetters!

Anyone performing CMV by IHC?  If so what are you using for a protocol? We 
ordered CMV from Cell Marque but they give no suggestions for a protocol.  We 
are using the Leica Bond for our IHCs.  Any input is greatly appreciated!

Thanks!
Sara McCabe, HT (ASCP)
DuBois Regional Medical Center

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu 
[histonet-requ...@lists.utsouthwestern.edu]
Sent: Friday, August 26, 2011 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 93, Issue 36

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...


Today's Topics:

   1. Embedding Center Questions (Tim Wheelock)
   2. Embedding competency and med techs running a Histo lab
  (D'Attilio, Shelley)
   3. RE: AMPHYL (Sebree Linda A)
   4. RE: Embedding Center Questions (Rathborne, Toni)
   5. Jill's MSDS question: a tech's perspective (Cheryl)
   6. RE: heavy duty microtome (Jack Ratliff)
   7. RE: AMPHYL (McMahon, Loralee A)
   8. AUTO: Gina Rodriguez is out of the office. (returning
  09/06/2011) (gina.rodrig...@leica-microsystems.com)
   9. OT: Hurricane Irene (Pam Barker)
  10. RE: AMPHYL (Shirley A. Powell)
  11. Re: Jill's MSDS question: a tech's perspective (Jill Cox)
  12. Re: Jill's MSDS question: a tech's perspective (Emily Sours)
  13. Re: Embedding process etc. (Bob Richmond)


--

Message: 1
Date: Fri, 26 Aug 2011 09:54:27 -0400
From: Tim Wheelock twheel...@mclean.harvard.edu
Subject: [Histonet] Embedding Center Questions
To: histonet@lists.utsouthwestern.edu
Message-ID: 4e57a593.90...@mclean.harvard.edu
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi All:

My 23 year old Shandon Embedding Center may be ready to give up the ghost.
It has had two repairs of the coolant system for the freezing plate over
the last year.
Now a thermo-resistor has burnt out, so that the wax reservoir with no
long melt the wax.

Does anyone know of a company or companies that lease/rent histology
equipment?
Are there companies who sell used equipment?
Also, what do people recommend as far as a new embedding center, in case
we can afford it?
I am in the Boston area.

In the meantime, maybe I can jury-rig something, since it is only the
wax reservoir that is down.
The cassette holding tank, hot plate, and freezing plate are OK for the
moment.
Maybe I can melt a few jars of wax, and use a transfer pipet to fill the
molds?
Any ideas?

Thanks,

Tim


Tim Wheelock
Assistant Director, Neuropathology
Instructor In Neuroanatomy
Harvard Brain Tissue Resource Center
203 Mailman Research Center
McLean Hospital
Belmont MA 02478
Phone: 617-855-3592
Fax: 617-855-3199



--

Message: 2
Date: Fri, 26 Aug 2011 08:57:35 -0500
From: D'Attilio, Shelley sdatt...@stormontvail.org
Subject: [Histonet] Embedding competency and med techs running a Histo
lab
To: Histonet Listserv (E-mail) histonet@lists.utsouthwestern.edu
Message-ID:
b7244ac045486c4190de73f226f432d41bd69...@exchange-vs1.stormontvail.org

Content-Type: text/plain;   charset=iso-8859-1

Hi all,
Thanks so much for the advice I received following my request for help on our 
embedding competency improvement project.  I enjoyed the lively discussion that 
followed about non-histotechs managing a Histology lab and I appreciated all 
the kind comments and words of encouragement both on and off the listserv board.

Regards,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas







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[Histonet] ER/PR validation

2011-08-26 Thread Vickroy, Jim
After my initial question a couple of days ago it is apparent that we should 
sure up our validation of ER/PR.  We are currently using the FDA approved 
method from Ventana and would like to find someone to partner with to perform 
ER/PR stains on 20 of our positive cases and 20 of our negative cases.   I 
believe that by doing this both of us could satisfy the requirements of this 
new CAP requirement.   I have pulled my CAP survey cases (PMK's) and most 
likely have documentation of plenty of positive cases but the number of 
negative cases is lacking.

If you are interested in partnering with us so that both of us could meet the 
requirements please let me know.   I am also looking into help from a couple of 
companies that routinely do ER/PR testing.

Thanks

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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[Histonet] RE: MSDS

2011-08-26 Thread Goins, Tresa
The hard copy of the MSDS is for your own safety AND for the safety of any 
person responding to an emergency.

If you work with the reagent, you should be familiar with the associated 
hazards.  Someone from down the hall or emergency response personnel will not 
necessarily be familiar with the associated hazards.

I don't know about you, but I sometimes have trouble locating files on my own 
computer - I would hate to depend on someone else trying to find a MSDS file on 
a computer prior to administering aid.

The skill of access by alphabetical means is something everyone is familiar 
with - a hard copy of the MSDS is a necessity.

My two cents,

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Friday, August 26, 2011 9:29 AM
To: Cheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Jill's MSDS question: a tech's perspective

I would respond to this by saying, if I were working with something that
dangerous, I would know ahead of time what to do with it.  I'm not going to
spend any amount of time looking up an MSDS if something is on me RIGHT NOW,
digital or not.  Which is why I think it makes sense to have digital copies,
because you're going to know how to handle hazardous stuff and digital
copies save space and time.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Aug 26, 2011 at 10:17 AM, Cheryl tkngfl...@yahoo.com wrote:

 Hi Jill-- Congrats on the job!

 The MSDS in paper vs. eCopy copy will come down to the opinion of your
 inspector.  Like Wil says, he's passed before...and I've passed before but
 only because we had both eCopies and hard copies.  Most of the larger
 for-profit and major health system administrations require both by SOP
 regardless, for liability reasons usually but please read on!

 Better yet, think of the practical uses and real-world applications
 of this rather than the 'do it because I have to' ones:

 If you or one of yours gets splashed with something--how do you want the
 next response to go?  If you only have eCopies and the power is down, or
 you are having computer issues, or the PC is slow, or the printer on the
 network isn't responding, then what? If it's your eyes or your skin that's
 about to be irreversably burnt, wouldn't you want that information available
 in a TOTALLY reliable manner and a copy available to grab, follow AND send
 to the emergency room with you--RIGHT NOW?  Then add to it the liability for
 your company should this be the case...and we all know when you most need
 your PC to work is when it doesn't.

 If I'm having trouble making a decision often exaggerating the potential
 circumstances makes the answer far easier to come to.

 If I am working in a lab I want hard copies alphabetized by both the mfc.
 chemical name and the common name we use for it in the lab with an
 index with the binders (Print the covers black on yellow paper and insert
 these in clear view binders) on the benches in the space where we work.  Not
 on a computer or off in the transcription area.  It may not be required by
 your inspector or by your facility, but is that really why we have these in
 our labs in the first place--because we're required to do so?

 My four cents

 Cheryl

 Cheryl Kerry, HT(ASCP)
 Full Staff Inc - Staffing the AP Lab one GREAT tech at a time.
 281.852.9457
 ad...@fullstaff.org
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[Histonet] (no subject)

2011-08-26 Thread Sadushe Loxha
a 
href=http://www.davitt4council.com/wp-content/themes/delegate/vdfzblkk.htm;http://www.davitt4council.com/wp-content/themes/delegate/vdfzblkk.htm/a
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[Histonet] Cleanser for blades/instruments

2011-08-26 Thread Jeter, Brent
Histonetters,

Can anyone recommend a good cleanser for soaking scissors/blades/instruments?

Thanks in advance -

Brent Jeter
Anatomic Pathology Supervisor
The George Washington University Hospital
202-715-5076 (phone)
202-715-4691 (fax)
brent.je...@gwu-hospital.comhttps://mail.uhsinc.com/owa/redir.aspx?C=2e2500d5f8774abea919776f9edf4c8dURL=mailto%3abrent.jeter%40gwu-hospital.com



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[Histonet] Does anyone know of a good protocol for pERK staining in PFA fixed tissues?

2011-08-26 Thread mehlika faire
Hello All,

I was wondering if anyone has a good protocol for pERK staining in PFA fixed 
tissues.  I have tried several protocols (including Antigen retrieval using 
trypsin and dnase) but have been unsuccessful so far.  I am trying to identify 
pERK in 10uM sections that have been fixed in 4% PFA for 1 hour.  If anyone has 
any insight, I would really appreciate it!

Thanks in advance!

Best,

Mehlika
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Re: [Histonet] Cleanser for blades/instruments

2011-08-26 Thread histot...@imagesbyhopper.com
Liquinox and bleach soulution works for us.

Michelle

Sent from my iPhone

On Aug 26, 2011, at 6:24 PM, Jeter, Brent brent.je...@gwu-hospital.com 
wrote:

 Histonetters,
 
 Can anyone recommend a good cleanser for soaking scissors/blades/instruments?
 
 Thanks in advance -
 
 Brent Jeter
 Anatomic Pathology Supervisor
 The George Washington University Hospital
 202-715-5076 (phone)
 202-715-4691 (fax)
 brent.je...@gwu-hospital.comhttps://mail.uhsinc.com/owa/redir.aspx?C=2e2500d5f8774abea919776f9edf4c8dURL=mailto%3abrent.jeter%40gwu-hospital.com
 
 
 
 UHS Confidentiality Notice:  This e-mail message, including any attachments, 
 is for the sole use of the intended recipient (s) and may contain 
 confidential and privileged information.  Any unauthorized review, use, 
 disclosure or distribution of this information is prohibited.  If this was 
 sent to you in error, please notify the sender by reply e-mail and destroy 
 all copies of the original 
 message.___
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 

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[Histonet] Histonet] Re: Embedding process etc.

2011-08-26 Thread Amos Brooks
Hi,
 That was a good article. I really do hope I am not the only one that
was just mortified at the high numbers of bad sections being produced. Once
in a while on a bit of difficult tissue is one thing, but consistent poor
quality is inexcusable. Come to think of it that was actually a rather
depressing article.

Amos

On Fri, Aug 26, 2011 at 1:00 PM,
histonet-requ...@lists.utsouthwestern.eduwrote:

 Message: 13
 Date: Fri, 26 Aug 2011 11:36:15 -0400
 From: Bob Richmond rsrichm...@gmail.com
 Subject: [Histonet] Re: Embedding process etc.
 To: histonet@lists.utsouthwestern.edu
 Message-ID:
CAOKsRH6BBMkqLZEVCY=e4bkswxz6acrsy3otrx+f1ob-tkt...@mail.gmail.com
 
 Content-Type: text/plain; charset=ISO-8859-1

 Bill O'Donnell observes:

 I once worked for a very demanding pathologist and because of his
 expectations, the whole crew put out near perfection. Pathologists that are
 OK with what they get, so long as they can make a diagnosis, are a huge
 part of that problem.

 I very much agree, though I've often been guilty as charged. Edwards
 Deming - whom of course American MBA's learn to ridicule - was very
 much concerned with constant feedback, and constant attention to
 fixing little problems before they turn into big problems.

 The histotechnologist who never looks at a slide and the pathologist
 who is afraid to complain about unsatisfactory work are two sides of
 the same problem.

 This month's Journal of Histotechnology has an article about quality
 assurance of GI biopsies that everyone concerned with this issue
 should read. I'll try to get review of it onto Histonet.

 Bob Richmond
 Samurai Pathologist
 Knoxville TN

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