[Histonet] Embedding Centre
We are looking at purchasing a Thermo Histostar. What are your thoughts and experience with this embedder. Cheers Grant Madill Laboratory Technician Animal Disease Surveillance Laboratory Biosecurity Queensland Department of Employment, Economic Development and Innovation 203 Tor Street, Toowoomba Qld 4350 We're behind the Bid! GOLD COAST 2018 - XXI COMMONWEALTH GAMES CANDIDATE CITY www.goldcoast2018bid.com DISCLAIMER The information contained in the above e-mail message or messages (which includes any attachments) is confidential and may be legally privileged. It is intended only for the use of the person or entity to which it is addressed. If you are not the addressee any form of disclosure, copying, modification, distribution or any action taken or omitted in reliance on the information is unauthorised. Opinions contained in the message(s) do not necessarily reflect the opinions of the Queensland Government and its authorities. If you received this communication in error, please notify the sender immediately and delete it from your computer system network. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Bouin's without the picric acid?
Thanks for all the replies. Some alternatives or Bouin's substitutes have been suggested. Whether they are OK with Bielschowsky’s and Gallyas Silver Staining and the like remains to be determined. Whatever fixative I end up using must be also OK with Thiolfavin-S T, FluoroJadeB and immunohistochemistry/fluorescence. My problem with PFA is that it can destroy some epitoptes and some of my antibodies are directed against sensitive epiptopes (glycoproteins). I need an alternative fixative which isn't going to cause trouble for a broaf range of staining techniques. I have, with my whole mounts brains, used PFA for a 10 minute fix and then 10 minute MeOH postfix without problems but 10 minutes is very different to overnight.. Thanks for the advice. (John Kiernan, if you still out there, I would love to hear from you!) -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE
This happened to me several months ago. Our lab always has high humidity. If you are going to continue to use clear rite with humidity in the air, I would change it everyday. Also, change out your 100% because it's inevitably getting water in it too. I just switched my whole lab back to xylene...at least you can see the water in it =) Good Luck Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anuradha shrivastava Sent: Wednesday, September 14, 2011 9:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Hello everybody, Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50 Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper’s bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE
We use ProPar on our stainer. We rotate our 4 100% alcohols that are after eosin after every 3rd rack and we have stopped the leaching of eosin in our last ProPars. Not a big fan of exposing my staff to xylene unless I absolutely have to. Jen Campbell On Thu, Sep 15, 2011 at 9:44 AM, sdys...@mirnarx.com wrote: This happened to me several months ago. Our lab always has high humidity. If you are going to continue to use clear rite with humidity in the air, I would change it everyday. Also, change out your 100% because it's inevitably getting water in it too. I just switched my whole lab back to xylene...at least you can see the water in it =) Good Luck Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anuradha shrivastava Sent: Wednesday, September 14, 2011 9:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Hello everybody, Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50 Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper’s bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lysol IC
I was told that the Lysol I.C. is being discontinued also. Is anyone using Virex? You can also use Lysol.I.C. or Virex to decontaminate the Ventana platforms -Original Message- Date: Thu, 25 Aug 2011 12:56:20 To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE
I don't know your specific protocol and what stains you are using (vendor), but we switched a long time ago to CitriSolv, another xylene substitute and have had not problems in our Leica Autostainer XL. We don't change the CitriSolv every time and sometimes filter it. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anuradha shrivastava Sent: Wednesday, September 14, 2011 10:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Hello everybody, Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50 Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper's bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lysol IC
I just received some Lysol IC from Lab Safety Supply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Thursday, September 15, 2011 7:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol IC I was told that the Lysol I.C. is being discontinued also. Is anyone using Virex? You can also use Lysol.I.C. or Virex to decontaminate the Ventana platforms -Original Message- Date: Thu, 25 Aug 2011 12:56:20 To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern. edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] warthin starry fading
Hi all! Today I stained Warthin Starry for Hp. This time I was lucky to have the first time purchased control tissue (Spirochetes control from Sigma) for comparison. In the package there was also a ready stained slide with really nice black spirochetes in yellow-brownish tissue/nuclei. This was stained with a Sigma Steiner Kit, but I thought my Warthin Starry should look the same. So, after staining, the patient tissue shows nice Hp in yellow/brownish background, but nuclei didn't stain. The control tissue had black-sprinkled nuclei and no spirochetes at all. I was a little bit disappointed, but at least the patient tissue was ok for diagnosis. A few hours later I looked at the control slide again and found, that the whole black staining had faded till a few speckles. (Patient slide was out of reach to look at) Does anybody know why this fading did happen? I have no information about the fixation and processing of the control slides, but thought it must fit for FFPET. The only point is, that we received the slides several months before. Is aging a factor? Staining protocol in brief: Everything diluted in pH 4 water, made with citric acid. 30 min 1% AgNO3 5-7 min developer (2% AgNO3+gelatine+Hydrochinon) Waterrinse Dehydration, coverslipping Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE
As I understand it, the Eosin is actually leached out by the ETOH that has been contaminated by water. The Propar that follows will show droplets of water in the bottom just as xylene does. Joyce Friedland We can't control the wind but we can adjust our sails. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] warthin starry fading
I think I've found something. Obviously the staining result is sensitive for special coverslipping medium. Patient slide was coverslipped with Pertex, control slide was coverslipped with Eukit. Interesting Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Donnerstag, 15. September 2011 17:17 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] warthin starry fading Hi all! Today I stained Warthin Starry for Hp. This time I was lucky to have the first time purchased control tissue (Spirochetes control from Sigma) for comparison. In the package there was also a ready stained slide with really nice black spirochetes in yellow-brownish tissue/nuclei. This was stained with a Sigma Steiner Kit, but I thought my Warthin Starry should look the same. So, after staining, the patient tissue shows nice Hp in yellow/brownish background, but nuclei didn't stain. The control tissue had black-sprinkled nuclei and no spirochetes at all. I was a little bit disappointed, but at least the patient tissue was ok for diagnosis. A few hours later I looked at the control slide again and found, that the whole black staining had faded till a few speckles. (Patient slide was out of reach to look at) Does anybody know why this fading did happen? I have no information about the fixation and processing of the control slides, but thought it must fit for FFPET. The only point is, that we received the slides several months before. Is aging a factor? Staining protocol in brief: Everything diluted in pH 4 water, made with citric acid. 30 min 1% AgNO3 5-7 min developer (2% AgNO3+gelatine+Hydrochinon) Waterrinse Dehydration, coverslipping Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Phosphogard/guard
Has anyone heard of or used this for protection of phosphorylation sites during IHC? My manager wants to try it. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Bouin's without picric acid
Davidson's fixative is made of three parts of tap water, three parts alcohol (you can use waste processing alcohol), two parts 37% formalin (not neutral buffered formalin) and one part glacial acetic acid. I have references if anyone wants them - they're pretty obscure. Bob Richmond Samurai Pathologist Knoxville TN Tyrone - I have been working with fish (whole and pieces and parts) for years. The fixative of choice for whole fish as been Davidson's. It contains formalin, alcohol, acetic acid and water. Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laura Miller is Out of the Office.
I will be out of the office starting 09/15/2011 and will not return until 09/22/2011. I am attending the National Society of Histotechnology meeting in Cincinnati, OH. I will have limited access to email. I will respond to your message as soon as possible. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC ?
Hello all, So I just realized I am out of my block after I have done HIER. Can I leave the slides in buffer in the fridge or something overnight. The block will be here at 10am tomorrow morning... She the slides be ok or do I need to re-cut all of them and start over (please tell me this is not the case) =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC ?
Excellent questions, I get it all the time. Overnight, in buffer, in the fridge will be ok. Do not leave in water as that will obscure morphology. Will On Sep 15, 2011, at 12:40 PM, sdys...@mirnarx.com wrote: Hello all, So I just realized I am out of my block after I have done HIER. Can I leave the slides in buffer in the fridge or something overnight. The block will be here at 10am tomorrow morning... She the slides be ok or do I need to re-cut all of them and start over (please tell me this is not the case) =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC ?
I have left them overnight in PBS in the refrigerator without any problem. It is always better to do that and maybe replace a few that the whole bunch of slides. René J. --- On Thu, 9/15/11, sdys...@mirnarx.com sdys...@mirnarx.com wrote: From: sdys...@mirnarx.com sdys...@mirnarx.com Subject: [Histonet] IHC ? To: histonet@lists.utsouthwestern.edu Date: Thursday, September 15, 2011, 3:40 PM Hello all, So I just realized I am out of my block after I have done HIER. Can I leave the slides in buffer in the fridge or something overnight. The block will be here at 10am tomorrow morning... She the slides be ok or do I need to re-cut all of them and start over (please tell me this is not the case) =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks
Hello Histogurus, Thank you very much for valuable advice. Anu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Warthin starry fading
Hi Gudrun I had this same problem some years back. I changed from a previous mounting medium to Merck Entellan New. The HE's were fine but when I did warthin-starry staining they were completely faded the next day. I thought it must be the new mounting medium so I bought some Pertex and ran 2 control slides using each mounting medium and it was obvious that the Entellen was at fault. Only use Pertex now and find it very good. Cheers Grant Madill Laboratory Technician Animal Disease Surveillance Laboratory Biosecurity Queensland Department of Employment, Economic Development and Innovation 203 Tor Street, Toowoomba Qld 4350 * PO Box 102, Toowoomba Qld 4350 * +61 7 4688 1363 * +61 7 4688 1195 *grant.mad...@deedi.qld.gov.au mailto:name.n...@deedi.qld.gov.au Business Information Centre 13 25 23 www.deedi.qld.gov.au http://www.deedi.qld.gov.au/ We're behind the Bid! GOLD COAST 2018 - XXI COMMONWEALTH GAMES CANDIDATE CITY www.goldcoast2018bid.com DISCLAIMER The information contained in the above e-mail message or messages (which includes any attachments) is confidential and may be legally privileged. It is intended only for the use of the person or entity to which it is addressed. If you are not the addressee any form of disclosure, copying, modification, distribution or any action taken or omitted in reliance on the information is unauthorised. Opinions contained in the message(s) do not necessarily reflect the opinions of the Queensland Government and its authorities. If you received this communication in error, please notify the sender immediately and delete it from your computer system network. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
