Re: [Histonet] Regarding cartilage and bone staining
Hi Pathik, perhaps you could give us some background on what it is you are researching? On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology kraniofacialbiol...@gmail.com wrote: Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be used in place of the whole mount cartilage and bone staining that would stain the sections on a slide and works on cartilage and bone. This would also save the whole mouse to be used and many slides can also be used for other experiments. Regards Pathik ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Methenamine
I have tried to order methenamine and was told they no longer make it. We have tried many different companies that have it listed in their products but when you order it you are told it is no longer available. Does anyone know where we can get it or what is being used in its place. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 Memorial Healthcare System To the world I'm one, to one I'm the world This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Methenamine
We get ours from Sigma-Aldrich (it is called Hexamethylenetetramine, ACS reagent) Catalog Number 398160-250G. We received it last May, so I assume that they still make it. Website is http://www.sigmaaldrich.com/united-states.html. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, October 12, 2011 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Methenamine I have tried to order methenamine and was told they no longer make it. We have tried many different companies that have it listed in their products but when you order it you are told it is no longer available. Does anyone know where we can get it or what is being used in its place. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 Memorial Healthcare System To the world I'm one, to one I'm the world This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cat Scratch fever
Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, Mauger, Joanne mau...@email.chop.edu wrote: Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC-P compatible Serum amyloid P antibody against rat
Hello everyone, I'm in need for an anti-rat-Serum amyloid P antibody which is IHC-P compatible. Suggestions, anyone? Thank you very much in advance! Best, Till GSI Helmholtzzentrum für Schwerionenforschung GmbH Planckstraße 1 64291 Darmstadt www.gsi.de Gesellschaft mit beschränkter Haftung Sitz der Gesellschaft: Darmstadt Handelsregister: Amtsgericht Darmstadt, HRB 1528 Geschäftsführung: Professor Dr. Dr. h.c. mult. Horst Stöcker, Peter Hassenbach, Dr. Hartmut Eickhoff Vorsitzende des Aufsichtsrates: Dr. Beatrix Vierkorn-Rudolph Stellvertreter: Ministerialdirigent Dr. Rolf Bernhardt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
No one under 30 would have a clue what that joke means. Lol Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, Mauger, Joanne mau...@email.chop.edu wrote: Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cat Scratch fever
Google it and Ted Nugent is on the 1st page, although not the top spot. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/12/2011 7:19 AM, Galbraith, Joe wrote: No one under 30 would have a clue what that joke means. Lol Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, Mauger, Joannemau...@email.chop.edu wrote: Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
Try Ted Nugent, Inc. ...or you can get control slides from American Master Tech. http://www.americanmastertech.com/histology_control_slides.htm Brett -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
I was just watching his show last night! That's entertainment! Galbraith, Joe joseph-galbra...@uiowa.edu 10/12/2011 10:19 AM No one under 30 would have a clue what that joke means. Lol Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, Mauger, Joanne mau...@email.chop.edu wrote: Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bright M3500 microtome
Hello everyone, Looking for opinion about Bright M3500 microtome - any advantages in comparison to Microm HM325 or Leica RM2235? Thanks Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabsmailto:AGleiberman@cbiolabs.com This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Artisan barcode reading issue
Good Morning Histonetters! Is anyone else having issues with reagent cartridges not reading on the Artisan staining system? We have had this instrument for quite some time and have started having problems with the barcodes not reading - a random cartridge from a kit - not always the same one - pretty much no rhyme or reason. The area of read has been cleaned (several times) but this continues to be an issue. Thanks in advance- Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Annexin V IHC staining in mouse xenograft
Jan, Thank you for the input but I did get a lot of suggestions yesterday. Thank you all. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatan...@tgen.org website: www.tgen.org On 10/12/11 8:09 AM, Jan Shivers shive...@umn.edu wrote: Aprill, If you send us a list of the exact reagents and incubation times/dilutions that you use, someone on Histonet may be able to answer your question. Jan Shivers Univ. of Minnesota - Original Message - From: awatan...@tgen.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, October 11, 2011 12:11 PM Subject: [Histonet] Annexin V IHC staining in mouse xenograft Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between. I'm looking to see if anyone knows anything else to try. I'm trying to rearrange the protocol to put steps in different places to see if that works. At this point I'm not sure what else to try so any ideas would be great. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatan...@tgen.org website: www.tgen.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Annexin V IHC in mouse xenograft
Hi Aprill, I am not sure if you are using an antibody against Annexin V or if you are trying to stain with Annexin V (use it as a detection agent). Annexin V is a protein that binds to phosphatidyl serine. It is often used as a tool to detect early stages of apoptosis when the plasma membrane flips and exposes phosphatidyl serine on the outside of cells, whereas in normal live cells it would be on the inside. It is used eg. in flow cytometry on unfixed cells. Dead cells or permeabilized cells would be all positive as the reagent (Annexin V) would have access to the inside of cells and react with phosphatidyl serine in the inner leaflet of the membrane. I don't think it could work on tissues in paraffin sections. But somebody may prove me wrong... Sarka Lhotak McMaster University, Hamilton, Ontario ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Drying oven tempsIn-Reply-To=
For HE slides, we use the Leica ST5020 autostainer. We have two ovens. The first oven is set for 40 C, 20 min. Then the slide rack is transferred to the second oven at 60 C, 15 min. WE have recently incorporated placing all of our slide racks for HE in front of a small table top fan for 10-15 minutes prior to going onto the autostainer. This combination works well for us and does a great job of cutting down/ eliminating on the amount of nuclear bubbling artifact. We are not so stringent with our special stain slides. And IHC slides also have their own protocol. amysue __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing and embedding mice lungs.
Hello everyone! I have a student that wants to paraffin embed mice lungs, and I’ve only worked with bone tissue. I was wondering if someone in histo-land would be willing to share with me their protocol or their thoughts on how to proceed. The tissue will arrive inflated in a formalin fixative. Thank you, Bret Clough Texas AM Health Science Center College of Medicine Temple, TX. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MethenamineIn-Reply-To=
It is also available through Fisher. Hexamethylenetetramine H290-500 for a 500 gram bottle. amysue __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Population Served compentency requirements
I was wondering if anyone else was having to come up with a histology department competency with a focus on population served. From what I understand it is a Joint Commission requirement for our hospital annual competencies. We are having a hard time coming up with something because we do not work with the public, just their specimens. Any ideas??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fixing live brain slices
Does anyone know of a good method of fixing live brain slices that have been incubated in fluorescein diacetate? Upon drop-fixing the slices with 4%PFA I lose most to all fluorescent signal. Thank you for your time. Dave ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Regarding cartilage and bone staining
Hello I would like to ask how many time do you put the mice head in xylene after series of Ethanol wash in the way of making paraffin block. I also did all the steps as well, but for embryonic day 17-18, even after observing clearing in xylene and moving to the several times in Gurr/paraplast I find the head to be soft and the initial part of head from which we start to cut is white and when I removed the flecks of wax and touched it was still soft. I am not getting what to do. Pathik On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology kraniofacialbiol...@gmail.com wrote: Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be used in place of the whole mount cartilage and bone staining that would stain the sections on a slide and works on cartilage and bone. This would also save the whole mouse to be used and many slides can also be used for other experiments. Regards Pathik ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Regarding cartilage and bone staining
I'm not sure the size of the mouse head, but first of all the samples needs to be fixed properly I would let it stay in fixative for at least 48 hours. Then the sample would need to be decaled and that could take days depending upon the type of decal you choose to use. If you are processing the heads whole your processing cycle (depending upon the size of the samples) could range from 2 to 4 hours per station similar to something like this. I like to use three absolutes and 3 xylenes on bone samples and find that this works well. If the samples are over processed you can just cut back on the times. Liz 70% 2 hours 80% 2 hours 95% 2 hours 100% 2 hours 100% 2 hours 100% 2 hours Xylene 2 hours Xylene 2 hours Xylene 2 hours Paraffin 2 hours Paraffin 2 hours Paraffin 2 hours Paraffin 2 hours Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kraniofacial Biology Sent: Wednesday, October 12, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Regarding cartilage and bone staining Hello I would like to ask how many time do you put the mice head in xylene after series of Ethanol wash in the way of making paraffin block. I also did all the steps as well, but for embryonic day 17-18, even after observing clearing in xylene and moving to the several times in Gurr/paraplast I find the head to be soft and the initial part of head from which we start to cut is white and when I removed the flecks of wax and touched it was still soft. I am not getting what to do. Pathik On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology kraniofacialbiol...@gmail.com wrote: Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be used in place of the whole mount cartilage and bone staining that would stain the sections on a slide and works on cartilage and bone. This would also save the whole mouse to be used and many slides can also be used for other experiments. Regards Pathik ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Annexin V IHC staining in mouse xenograft
Hi, Have you considered running the antibody through some antibody purification beads. It isn't really a common histolgy thing to do, but the process isn't terribly difficult. It really sounds like a dirty antibody is at fault. It will probably result in a cleaner and more concentrated antibody. There are some commercially available kits to do this. I believe Invitrogen carries one and I'm almost sure Chemicon does too. Amos Message: 1 Date: Tue, 11 Oct 2011 17:11:38 + From: awatan...@tgen.org Subject: [Histonet] Annexin V IHC staining in mouse xenograft To: histonet@lists.utsouthwestern.edu Message-ID: cab9c6e3.925e%awatan...@tgen.org Content-Type: text/plain; charset=us-ascii Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between. I'm looking to see if anyone knows anything else to try. I'm trying to rearrange the protocol to put steps in different places to see if that works. At this point I'm not sure what else to try so any ideas would be great. Aprill Watanabe, B.S. Research Associate II ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP COMMON CHECKLIST
Hello every body, need your input for cap common checklist for PT(PROFICIENCY TESTING) evaluation in histology. we participate in Histoquip through CAP. want to know what other labs r doing? thanks a lot . anu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
