RE: [Histonet] Suggestions please...
You could buy some beef liver from the grocery store. Works great. From: lpw...@sbcglobal.net To: kjg...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sun, 23 Oct 2011 08:18:12 -0400 Subject: Re: [Histonet] Suggestions please... CC: Is there a slaughter house nearby? Call them, and have some documentation that you are from a university - such as a memo on a letterhead. Is there animal research at your university? Can they spare a rat? Try to do this right before class, so there is less autolysis. Put tissue in a plastic bag, and store in refrig for a few hours, until ready. Peggy Wenk, HTL(ASCP)SLS -Original Message- From: Komal Gada Sent: Saturday, October 22, 2011 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Suggestions please... Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the HE staining procedure? Thanks! Komal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi all Is anyone of you familiar with the staining of CD4 cells in minipigs in formalin fixed and paraffin embedded tissues? Joost TNO.NLhttp://www.tno.nl/ Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijnt...@tno.triskelion.nlmailto:joost.bruijnt...@tno.triskelion.nl Disclaimerhttp://www.tno.nl/tno/email/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cochineal mentioned in comic strip
I concur Peggy, great book and great article. Jan Mahoney HT(ASCP) Omaha, NE From: lpw...@sbcglobal.net To: Histonet@lists.utsouthwestern.edu Date: Sun, 23 Oct 2011 19:04:34 -0400 CC: Subject: [Histonet] cochineal mentioned in comic strip Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today’s 10/23/11 strip. Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween – which is where histology gets carmine dye for the mucicarmine stain. http://www.gocomics.com/jumpstart/2011/10/23 If you want to read a fascinating book about the the role of carmine in the exploration of the America’s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the “unions” of the dyeing industry back then – find or buy a copy of “A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire” by Amy Butler Greenfield, 2006. If you want a more abbreviated version, Anatech’s newsletter ”The Innovator” had an article about carmine in their Winter 2007 issue – all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head’s up – this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf No – I don’t get any money talking about the comic strip, the book or Anatech. I just think it’s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are my own.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cochineal mentioned in comic strip
The history of logwood, the source of hematoxylin, was similar. People have always wanted colors and dyestuffs were very precious in the millennia before the advent of practical organic chemistry. It's also interesting to consider that Carmine made from Cochineal is a popular red food coloring. It's likely that we've all eaten this bug byproduct. On 10/23/2011 6:04 PM, Lee Peggy Wenk wrote: Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today’s 10/23/11 strip. Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween – which is where histology gets carmine dye for the mucicarmine stain. http://www.gocomics.com/jumpstart/2011/10/23 If you want to read a fascinating book about the the role of carmine in the exploration of the America’s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the “unions” of the dyeing industry back then – find or buy a copy of “A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire” by Amy Butler Greenfield, 2006. If you want a more abbreviated version, Anatech’s newsletter ”The Innovator” had an article about carmine in their Winter 2007 issue – all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head’s up – this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf No – I don’t get any money talking about the comic strip, the book or Anatech. I just think it’s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are my own.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cochineal mentioned in comic strip
Thanks for forwarding these links. I found the comic strip and Anatech article fascinating (in their own rights). Regards, Merced -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: Monday, October 24, 2011 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cochineal mentioned in comic strip The history of logwood, the source of hematoxylin, was similar. People have always wanted colors and dyestuffs were very precious in the millennia before the advent of practical organic chemistry. It's also interesting to consider that Carmine made from Cochineal is a popular red food coloring. It's likely that we've all eaten this bug byproduct. On 10/23/2011 6:04 PM, Lee Peggy Wenk wrote: Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today’s 10/23/11 strip. Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween – which is where histology gets carmine dye for the mucicarmine stain. http://www.gocomics.com/jumpstart/2011/10/23 If you want to read a fascinating book about the the role of carmine in the exploration of the America’s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the “unions” of the dyeing industry back then – find or buy a copy of “A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire” by Amy Butler Greenfield, 2006. If you want a more abbreviated version, Anatech’s newsletter ”The Innovator” had an article about carmine in their Winter 2007 issue – all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head’s up – this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf No – I don’t get any money talking about the comic strip, the book or Anatech. I just think it’s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are my own.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana's OptiView Detection
Hello All, Is anyone using Ventana's OptiView detection kit? If so please let me know what you impression is. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used block cabinets
Hello, I work for the Ohio Biorepository; we have about 1 1/2 million FFPE specimen in-house and are looking for better storage of our blocks. Does anyone know where I could buy reasonably priced cabinets, in large quantity (somewhere between 500-1000 cabinets)? Any and all advice would be appreciated. Thanks! Best, Adrienne Anderson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DISPOSAL OF SPECIMENS
Hi histonetters what does everyone currently do with the vials the Pathologists empty when doing gross? Ours goes in biohazard bags, but someone mentioned to me they the labels should be taken off and then the vials put in the biohazard bag. If we did this we would just be taking off labels all day long. I have worked at 2 hospitals in my 35 years of experience and we have never done this. If we started this look at the clinical labs with blood specimens. Isn't there some sort of confidential agreement between the hospitals and hazard material companies? I would love to know your thoughts. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job Opening in Southern California
A part-time entry level histotech position is available in our laboratory. Please email or fax resume to: Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 plu...@biopath.org 714-755-2984 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana's OptiView Detection
Hi Matt, I have worked a bit with this detection kit and my impression is that is extremely sensitive and should be able to boost signal considerably. I have also been able to shave off an average of 30 minutes off several stains. (Cyclin D1, which is one of our longer stains at 4 hours is down to 2.5) You can further enhance the stain (or shorten the time) with the amp kit, but I did not find it necessary. I thought it was particularly good on nuclear stains. There is also a good deal of flexibility with the kit and you should be able to get a great stain in less time. Hope that was helpful. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Content-Type: text/plain; charset=us-ascii Hello All, Is anyone using Ventana's OptiView detection kit? If so please let me know what you impression is. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking Beta-Amyloid not needing FA pretreatment
Hi everyone, I asked this question a long time ago but would like to see if anything new is available. We'd like to totally automate our Beta-Amyloid antibody but in order to do so, we need an antibody that does not require Formic Acid pretreatment. Anyone (especially vendors) no of any on the market? Thanks, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Seeking Beta-Amyloid not needing FA pretreatment
We use Dako's Beta Amyloid with high pH heat retrieval. Works very well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [lseb...@uwhealth.org] Sent: Monday, October 24, 2011 3:38 PM To: Histonet@lists.utsouthwestern.edu Cc: Normington Lacy Subject: [Histonet] Seeking Beta-Amyloid not needing FA pretreatment Hi everyone, I asked this question a long time ago but would like to see if anything new is available. We'd like to totally automate our Beta-Amyloid antibody but in order to do so, we need an antibody that does not require Formic Acid pretreatment. Anyone (especially vendors) no of any on the market? Thanks, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Seeking Beta-Amyloid not needing FA pretreatment
We have success with 6E10 and 4G8 clones using citrate retrieval. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, October 24, 2011 3:39 PM To: Histonet@lists.utsouthwestern.edu Cc: Normington Lacy Subject: [Histonet] Seeking Beta-Amyloid not needing FA pretreatment Hi everyone, I asked this question a long time ago but would like to see if anything new is available. We'd like to totally automate our Beta-Amyloid antibody but in order to do so, we need an antibody that does not require Formic Acid pretreatment. Anyone (especially vendors) no of any on the market? Thanks, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processor stopped - help!
Hi all, When I arrived the lab this morning I found the processor basket with my cassettes stuck between the two paraffin tanks. I suppose it went through the first cycle but not the second one, so its missing 1.5 hrs of paraffin infusion. Should I put them back in hot paraffin for 1.5 hours or what can I do to save the samples? Thanks for your advice, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] We need someone to test two antibodies.
Hello Histonetters We are in need of someone who can test two antibodies: CD71 and CD74 for us. --IHC Frozen Tissue (Methanol fixed tonsil) for both antibodies. --ImmunoFluorescence - Frozen tissue (Methanol fixed) We have FITC conjugated antibodies. Please contact us at : bade...@gmail.com If you need more information, please visit our web site: www.ImmunoBioScience.com and look for monoclonal antibodies catalog # MM-1027 and MM-1029 Thank you. Bader -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processor stopped - help!
I am glad to hear the samples will be fine. Thanks everyone! Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: processor stopped - help!
Yes, that should work fine. Best! j From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bode...@mcri.edu.au] Sent: Monday, October 24, 2011 6:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] processor stopped - help! Hi all, When I arrived the lab this morning I found the processor basket with my cassettes stuck between the two paraffin tanks. I suppose it went through the first cycle but not the second one, so its missing 1.5 hrs of paraffin infusion. Should I put them back in hot paraffin for 1.5 hours or what can I do to save the samples? Thanks for your advice, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Suggestions please...
Komal - I once found a lymph node in a roast from the market. A quick home version fixative with vinegar and isopropyl kept it good until I could get it into formalin the next day, it cut and demonstrated great. If by chance there is a university with an agriculture program, that could be a source of tissue also, and autolysis could be minimized. Rick T. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
