[Histonet] AUTO: Ramona Nelson is out of the office. (returning 11/01/2011)
I am out of the office until 11/01/20= 11. I = will respond to your message when I return. Note: This is an automated response to your message &quo t;Histonet Digest, Vol 95, Issue 33"= sent on = 10/28/2011 11:53:15 AM. This is the only notification you will receive wh= ile this person is away. _ = ** * IMPORTANT MESSAGE FOR RECIPIENTS INTHE U.S.A.: This me= ssage may constitute an advertisement of a BD group's products or services = or a solicitation of interest in them. If this is such a message and you = would like to opt out of receiving future advertisements or solicitations f= rom this BD group, pleaseforwardthise-mailto optoutbygr...@bd.com. *** Th= is message (which includes any attachments) is intended only for the design= ated recipient(s). It may contain confidential or proprietaryinformatio=nand may be subject to the attorney-client privilege or other confidenti= ality protections. If you are not a designated recipient, you may not re= view, use, copy or distribute this message. If you received this in error= , please notify the sender by reply e-mail and delete this message. Thank you. * = ** Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Comp= any) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. * ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fixation, tissue processing for paraffin embedding and sectiong of the mouse eye
Anyone has a good protocol to fix, process, embed and section mouse eye? Prefered fixative is formalin. Any suggestions are welcome Thank you! Mesru Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I have been wondering what a new certified HTL in the Denver area might expect to be paid, this person has a couple of years of exclusive histology experience heavy on IHC, bright field and IF, and frozen sectioning expertise along with all other areas of histology. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, October 28, 2011 1:53 PM To: Langenberg Stacey ; caroline.pr...@uphs.upenn.edu ; kcasti...@frii.com ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] SALARY That sounds good to me! I have never been fortunate enough to have education weighted. Of course I know it depends on market, location, and cost of living. Makes me want to research and pack up! Joelle Sent from my Verizon Wireless BlackBerry -Original Message- From: Langenberg Stacey Date: Fri, 28 Oct 2011 15:22:26 To: ; ; Subject: Re: [Histonet] SALARY What about certification? Sent from myTouch 4G - Reply message - From: "Pratt, Caroline" To: "kcasti...@frii.com" , "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] SALARY Date: Fri, Oct 28, 2011 7:24 am I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SCSHT FALL MEETING NOV 4-5, 2011
Good afternoon, My last post had too many words in the title so you could not see it was about our fall meeting!!! If anyone is planning to come and has not registered, please email me and plan to come on! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] SALARY
That sounds good to me! I have never been fortunate enough to have education weighted. Of course I know it depends on market, location, and cost of living. Makes me want to research and pack up! Joelle Sent from my Verizon Wireless BlackBerry -Original Message- From: Langenberg Stacey Date: Fri, 28 Oct 2011 15:22:26 To: ; ; Subject: Re: [Histonet] SALARY What about certification? Sent from myTouch 4G - Reply message - From: "Pratt, Caroline" To: "kcasti...@frii.com" , "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] SALARY Date: Fri, Oct 28, 2011 7:24 am I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] antibody who's who?
Jason Look at the images, clone, working conditions, sample concentration and size of the antibodies if all of these conditions are the same, especially if they are showing the same image, then its likely that the antibodies are the same. If any of those conditions are different then they could be different antibodies. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jason Madore Sent: Friday, October 28, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody who's who? Hello, We are in the process of testing multiple antibodies against the same target(s). I can't figure out though if two companies are selling the same antibody, or rather, as I know they are repackaging and selling the same antibodies, I can't tell who selling who's antibody. If anyone has any suggestions they would be greatly appreciated. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antibody who's who?
Hello, We are in the process of testing multiple antibodies against the same target(s). I can't figure out though if two companies are selling the same antibody, or rather, as I know they are repackaging and selling the same antibodies, I can't tell who selling who's antibody. If anyone has any suggestions they would be greatly appreciated. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
It's all relative to your area and absolute dollars aren't applicable outside a given region. For instance, here in the San Francisco Bay Area we have so many biotech companies and large medical centers sopping up histotechs that the going rate for even minimal experience is about $35/hr. Long term employees and supervisors can make over $50/hr. However, the cost of living is sky high, and just about everyone commutes quite a ways so recruiting from outside the area is extremely difficult - once people see how expensive it is and how far out they may have to live to afford housing we end up with very few applicants. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 9:52 AM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
With all that said what are your going rates in this area that you are speaking. Kind Regards! John J Shelley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology Immediate Evals
If you have 4 immediate evaluations during a FNA are you all charging 4 immed. eval. charges 88172 and an 88108 for each pass (cytospins)? Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 95, Issue 34
Richard, You looking to hire a free agent...oh I mean a lead or supervisor:) Joshua Wray HT(ASCP), QIHC Lead Technician, IHC Ameripath-Indianapolis > Message: 6 > Date: Fri, 28 Oct 2011 12:52:23 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] SALARY > To: ,, > "Caroline Pratt" > Message-ID: <4eaaa586.7400.007...@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I think that's low. If you find a good candidate with years of experience I > would pay them whatever it takes to get them in the door. It's like "Free > Agency" in baseball; if you want a good player, you need to put the "money on > table". Histotechnologists are the most valuable employees in the laboratory > today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > "Pratt, Caroline" 10/28/2011 9:21 AM >>> > I would say $28 to $32 dollars an hour depending on experience and education. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > kcasti...@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SALARY
Really good analogy Richard! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I like your world. I have seen more places that try to price Histology in the same range as CLA's not even close to MLT's. Tom -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PMS2 Mismatch Repair
Hello Histonetters, Wondering if anyone is using PMS2 Mismatch Repair Enzyme from Novocastra/Leica. Have tried varied dilutions and retrievals without any luck. Any suggestions would be great! Thanks! Sara McCabe, BS, HT (ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OK We're Counting Down for SCSHT Meeting
OK All you SC and close to SC Histotechs! The fall meeting of the SCSHT is next weekend at Marina Inn at Grande Dunes in Myrtle Beach, SC. We only have 10 registrants right now, so I need to know who's coming but you just haven't sent in your registration It's fine, we want you to attend, we are trying to get a head count for the food, etc Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] need help with TMA
We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Varicella Zoster
Hi Histonet! Cell Marque offers a Varicella Zoster antibody in both concentrate and pre-dilute. Please contact your rep or customer service if you have any questions! Thank you, Lauren Hopson Technical Consultant 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8929 (916) 996-2353 (mobile) (916) 746-8989 (fax) www.cellmarque.com From Passion to Product to Patient.(r) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Friday, October 28, 2011 9:31 AM To: Donna Liaros Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Varicella Zoster abcam has a few Varicella Zoster strains to choose from... I haven't used them before, so I can't speak of how well they work or provide much insight to dilutions and protocols for the Ventana, but the other antibodies I've used from them work well. Drew Sent from my iPhone On Oct 28, 2011, at 12:11 PM, Donna Liaros wrote: > Anyone have any recommendations for a vendor that supplies a concentrated > antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it > on the Ventana Ultra. > The information transmitted in this email and any of its attachments is > intended only for the person or entity to which it is addressed and may > contain BioReference Laboratories proprietary information, which is > privileged, confidential, or subject to copyright belonging to BioReference > Laboratories. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited and may be unlawful. > If you received this in error, please contact the sender immediately and > delete and destroy the communication and all of the attachments you have > received and all copies thereof. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to save dried antibody?
I personally would not use it; you don't know how much loss of immunologic reactivity has occurred, and that might affect your project data. Buy a fresh supply, if possible. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: "sarah Tabatabaei" To: Sent: Friday, October 28, 2011 10:27 AM Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Varicella Zoster
abcam has a few Varicella Zoster strains to choose from... I haven't used them before, so I can't speak of how well they work or provide much insight to dilutions and protocols for the Ventana, but the other antibodies I've used from them work well. Drew Sent from my iPhone On Oct 28, 2011, at 12:11 PM, Donna Liaros wrote: > Anyone have any recommendations for a vendor that supplies a concentrated > antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it > on the Ventana Ultra. > The information transmitted in this email and any of its attachments is > intended only for the person or entity to which it is addressed and may > contain BioReference Laboratories proprietary information, which is > privileged, confidential, or subject to copyright belonging to BioReference > Laboratories. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited and may be unlawful. > If you received this in error, please contact the sender immediately and > delete and destroy the communication and all of the attachments you have > received and all copies thereof. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Varicella Zoster
Anyone have any recommendations for a vendor that supplies a concentrated antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it on the Ventana Ultra. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How to save dried antibody?
If the antibody has precipitated, it may be impossible to restore activity. The glycerol is present to keep the suspension in liquid form - and it sounds like you no longer have a liquid. Try adding an appropriate suspension buffer and keep your fingers crossed. If you do not have previous experience with this antibody and know what to expect regarding performance, I would discard it and re-order. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah Tabatabaei Sent: Friday, October 28, 2011 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to save dried antibody?
Not sure if others would call this a "lyophilized" situation. Dont know the specifics of what you initially recieved. Here's my best shot as I recall from using lyophilized antibodies doing IF for renal bx's a while back. Back then I used a PBS based diluent. A very low reconstitution amount, 50-100um. I typically used the diluent in the pipett tip to swirl the freeze dried/lyophilized antibody back together while warming the tube with my thumb as I held it. It worked well in that situation for me. Hope this helps. I'm sure there are others out here who do this every day and can offer more specifics? Best of luck. Kim Donadio From: sarah Tabatabaei To: "histonet@lists.utsouthwestern.edu" Sent: Friday, October 28, 2011 11:27 AM Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How to save dried antibody?
Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] SALARY
What about certification? Sent from myTouch 4G - Reply message - From: "Pratt, Caroline" To: "kcasti...@frii.com" , "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] SALARY Date: Fri, Oct 28, 2011 7:24 am I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] used cryostat opinion
I've had great success with the Microms and you can get some really good pricing on used units. That being said I've also used the Leica's and like the 1850. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histostar Embedding Station
I've had mine for about a year and have had no problems either. Carlos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SALARY
HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
