Re: [Histonet] Stripping antibodies
With quantum dots and a modern fluorescence microscope with channels it should be possible to label 2 or 3 antigens simultaneously, if you have all the right primaries and labelled secondaries. The company that sells you the quantum dot labelled secondaries or other amplification reagents should be in a position to tell you exactly what to do. Do they refund your hard-earned grant money if their expensive product fails to perform as advertized? Immunohistochemistry with non-fluorescent colours (available in brown, black, red and various blues) has advantages: (a) You do not need a fluorescence microscope. Modern fluorescence microscopes are very expensive; the old ones from the 1960s are no longer be good enough to provide publication-quality pictures. (b) Immunostained slides can be kept for many years in boxes at room temperature. (c) The colours do not fade in permanently mounted preparations. (c) You are not bound to use a commercially sold kit, which may not be optimized for your research. If you go by the book (= any book with references that you can follow up), you will do your multiple immunostains intelligently. Never follow a set of instructions without knowing or questioning the reason for ever step. John Kiernan Anatomy, UWO London, Canada. = = = On 30/10/11, Claire Weston cwes...@valasciences.com wrote: Thank you everyone for your advice, I really appreciate it! I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies. The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned. I think I will try several of these techniques and evaluate which works the best. Thanks again for all your help, Claire --- jkier...@uwo.ca wrote: From: John Kiernan jkier...@uwo.ca To: histonet@lists.utsouthwestern.edu, cwes...@valasciences.com Cc: Amos Brooks amosbro...@gmail.com Subject: Re: [Histonet] Stripping antibodies Date: Sun, 30 Oct 2011 12:09:58 -0400 Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada = = = = On 29/10/11, Amos Brooks amosbro...@gmail.com wrote: Hi, Stripping sites are usually found in the more seedy areas of large cities ... Oh wait you meant ... nevermind :-) If you developed the reaction with DAB, you may be in a difficult position. DAB is a really strong reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is clear (usually a couple of minutes). Unfortunately, I am not sure what effect this will have on the epitopes you are looking for, but this is usually what is used to clean precipitated DAB from instruments it should work on the tissue too. If you use another chromogen such as AEC it is much easier (to many people's dismay) to remove this end product. Using alkaline phosphatase will be easy to remove the chromogens as well. It would also be very easy to do this with fluorescent tags too. Just remove the coverslip and dip the slide in ethanol and it will remove the colored end product. Then just start over for the new antigen. Good luck, Amos On Fri, Oct 28, 2011 at 11:50 AM,
RE: [Histonet] SALARY
Histotechnologists are the most valuable employees in the laboratory today! THANK YOU DR. CARTUN!! Nancy Heath, HT (ASCP) Neuropathology Technician Neuropathology Department Rhode Island Hospital President - Rhode Island Society for Histotechnology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husbandwould of had to pay that out of pocket. I'm in New England. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Friday, October 28, 2011 2:25 PM To: Pratt, Caroline; Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY With all that said what are your going rates in this area that you are speaking. Kind Regards! John J Shelley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I agree :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, October 28, 2011 3:22 PM To: Podawiltz, Thomas; Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Must be Monday...
That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Florida Medical Directorship
What are the true regulations' in Florida as far as time required for a Medical Director to be on site at the lab ? I am finding conflicting information and would like to know what is truly required. Thanks Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problems with Frozen tissues
I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do HE and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stripping antibodies
Amos and John, I do not agree with John saying that antibodies could be easily removed by acidity pH1-2. High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining. Chris van der Loos Academic Medical Center Dept. of Pathology M2-230 Amsterdam The Netherlands Date: Sun, 30 Oct 2011 12:09:58 -0400 From: John Kiernan jkier...@uwo.ca Subject: Re: [Histonet] Stripping antibodies To: histonet@lists.utsouthwestern.edu, cwes...@valasciences.com Cc: Amos Brooks amosbro...@gmail.com Message-ID: 7640acec48487.4ead3...@uwo.ca Content-Type: text/plain; CHARSET=US-ASCII Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Must be Monday...
Yes it was this past weekend back when. My alarm clock went to DST Sunday morning. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 7:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: SALARY
Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 6 Date: Fri, 28 Oct 2011 12:52:23 -0400 From: Richard Cartun rcar...@harthosp.org Subject: RE: [Histonet] SALARY To: kcasti...@frii.com,Histonet@lists.utsouthwestern.edu, Caroline Pratt caroline.pr...@uphs.upenn.edu Message-ID: 4eaaa586.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 34 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well Joelle Weaver MAOM, BA, (HTL) ASCP Date: Mon, 31 Oct 2011 06:29:12 -0400 From: nhe...@lifespan.org To: jshel...@sanfordburnham.org; caroline.pr...@uphs.upenn.edu; rcar...@harthosp.org; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY CC: Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husbandwould of had to pay that out of pocket. I'm in New England. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Friday, October 28, 2011 2:25 PM To: Pratt, Caroline; Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY With all that said what are your going rates in this area that you are speaking. Kind Regards! John J Shelley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this
[Histonet] RE: need help with TMA
Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply gentle pressure to center of block and slide complex, cool to RT and repeat 1 or two times. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patricia F Lott Sent: Friday, October 28, 2011 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need help with TMA We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: SALARY
Still sounds good to me, at my first supervisor's job ( the credentials I had at the time were HTL + Bachelor's), and I made less than $23 per hour and worked an average of 16 hours a day with no OT or comp time that was ever granted ( though promised). I guess I am just trying to say, count your blessings. Having a school near by really seems to impact the supply and demand and the general hiring environment. What I have noticed also is that when you go somewhere and the cost of living is less the tax burden is greater, so it has always seemed to come out in the wash. Anyhow, all these salary revelations have really made me think, thanks for the postings. Joelle Weaver MAOM, BA, (HTL) ASCP From: tnma...@mdanderson.org To: histonet@lists.utsouthwestern.edu Date: Mon, 31 Oct 2011 09:25:58 -0500 Subject: [Histonet] RE: SALARY Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 6 Date: Fri, 28 Oct 2011 12:52:23 -0400 From: Richard Cartun rcar...@harthosp.org Subject: RE: [Histonet] SALARY To: kcasti...@frii.com,Histonet@lists.utsouthwestern.edu, Caroline Pratt caroline.pr...@uphs.upenn.edu Message-ID: 4eaaa586.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 34 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] background checks required by AHCA
I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_WhoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employees personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Pr on Ventana?
Hi Friends, If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you please contact me separately? I'd like some advice. Thank you Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 -Original Message- Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problems with Frozen tissues
Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. Kim From: Igor Deyneko igor.deyn...@gmail.com To: Histonet@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:56 AM Subject: [Histonet] Problems with Frozen tissues I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do HE and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide labeling
Hi, Just curious because this questions has come up multiple times from non-histology people so I thought I'd post it here. When labeling slides at microtomy what info do you include on the slide? I know the automated slide labelers that are out there will include patient name, surgical number etc.; but when handwriting slides as you cut what do you write on them? Currently we write the case number, part number and level as well as the cutters initials. After staining we put the permanent label on that has patient name and case number etc (hence two identifiers). Is there anyone out there who hand writes the patient name on the slide or a second identifier on it at the time of microtomy? Thanks for your help, Lisa Lisa V. Chg., IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] stripping antibodies
If your antibodies are labeled with quantum dots and you strip the antibodies (e.g. with low pH) you will no longer have labeled targets in your tissue. Not clear if you understood this from your post. Message: 2 Date: Sun, 30 Oct 2011 11:58:19 -0700 From: Claire Weston cwes...@valasciences.com Subject: Re: [Histonet] Stripping antibodies ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Stripping antibodies
Thanks Chris. I didn't know there were antibodies with such strong adhesion. John. = = = On 31/10/11, C.M. van der Loos c.m.vanderl...@amc.uva.nl wrote: Amos and John, I do not agree with John saying that antibodies could be easily removed by acidity pH1-2. High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining. Chris van der Loos Academic Medical Center Dept. of Pathology M2-230 Amsterdam The Netherlands Date: Sun, 30 Oct 2011 12:09:58 -0400 From: John Kiernan jkier...@uwo.ca Subject: Re: [Histonet] Stripping antibodies To: histonet@lists.utsouthwestern.edu, cwes...@valasciences.com Cc: Amos Brooks amosbro...@gmail.com Message-ID: 7640acec48487.4ead3...@uwo.ca Content-Type: text/plain; CHARSET=US-ASCII Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: slide labeling
Hi Lisa - We hand write our labels, but do so prior to cutting sections. The accession numbers [unique to every slide] are read from a printed data sheet and written on the slides. This allows verification that the numbers match [slide and block] as the tissues are cut. If the numbers are written at microtomy I see it as a source of possible labeling errors. All blocks and slides are processed in numerical order so there is a number check at embedding, at cutting and at final labeling. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Monday, October 31, 2011 10:09 AM To: 'histonet@lists.utsouthwestern.edu'; 'histonet-requ...@lists.utsouthwestern.edu'; 'histonet-boun...@lists.utsouthwestern.edu' Subject: [Histonet] slide labeling Hi, Just curious because this questions has come up multiple times from non-histology people so I thought I'd post it here. When labeling slides at microtomy what info do you include on the slide? I know the automated slide labelers that are out there will include patient name, surgical number etc.; but when handwriting slides as you cut what do you write on them? Currently we write the case number, part number and level as well as the cutters initials. After staining we put the permanent label on that has patient name and case number etc (hence two identifiers). Is there anyone out there who hand writes the patient name on the slide or a second identifier on it at the time of microtomy? Thanks for your help, Lisa Lisa V. Chg., IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] background checks required by AHCA
Everyone employed in our hospital undergoes periodic background checks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Monday, October 31, 2011 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] background checks required by AHCA I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_W hoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employee's personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] The SC Histo Meeting in Myrtle Beach, SC
November 4-5, 2011 is the SC Society of Histotechnology meeting at Marina Inn at Grande Dunes in Myrtle Beach, SC. Everyone is still invited even if you have not pre-registered! Gayle Callis is presenting 2 workshops and we will have good food and good fellowship If you think you are coming, please email me back so we can get a head count!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Automated coverslipper with Ventana labels
Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated coverslipper with Ventana labels
Hi Teri, And welcome to San Diego :-) My experience is, that you cannot leave the slides with the Ventana labels sitting in xylene for too long before coverslipping. I believe it is just how these labels are and doesn't have much to do with the coverslipper itself. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Monday, October 31, 2011 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated coverslipper with Ventana labels Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Field Histology Tech FL/GA/AL
Good Afternoon, New Position Alert! Our client is a world leader in Cancer Diagnostics who is currently seeking Histotechs who have experience working with IHC analyzers that would be interested in Field Support roles. We currently have an opening covering Northern FL, AL, and part of GA. The Position Offers: - Outstanding Base Salary and Competitive Bonus! - Gold Standard Benefits including but not limited to Medical, Cell Phone, Laptop, Car Allowance, Expenses, 401k, Paid Vacation! - Opportunity for Career Advancement! If you are interested in learning more please contact me directly at 800.875.6188 ext. 103 or m...@personifysearch.com Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
Not so good when you fall into a higher tax bracket and uncle sam takes 25% of your salary and the insurance with dental weekly would have cost me around $232.55 a week...no thanks From: joelle weaver [mailto:joellewea...@hotmail.com] Sent: Monday, October 31, 2011 10:35 AM To: Heath, Nancy L.; jshel...@sanfordburnham.org; caroline.pr...@uphs.upenn.edu; rcar...@harthosp.org; kcasti...@frii.com; Histonet Subject: RE: [Histonet] SALARY I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well Joelle Weaver MAOM, BA, (HTL) ASCP Date: Mon, 31 Oct 2011 06:29:12 -0400 From: nhe...@lifespan.org To: jshel...@sanfordburnham.org; caroline.pr...@uphs.upenn.edu; rcar...@harthosp.org; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY CC: Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husbandwould of had to pay that out of pocket. I'm in New England. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Friday, October 28, 2011 2:25 PM To: Pratt, Caroline; Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY With all that said what are your going rates in this area that you are speaking. Kind Regards! John J Shelley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcasti...@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM I would say $28 to $32 dollars an hour depending on experience and education. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kcasti...@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only
RE: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus
allison, we do all of our alcian blue on the machine. we love it!! anita dudley providence hosp mobile, ala From: allison_sc...@hchd.tmc.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 21 Oct 2011 14:00:44 + Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus Hello to all in histo land. For those of you that have the ventana special stains machine, can the alcian blue 2.5 be done on the machine? I am not sure if they have kits for this stain. Also is anyone using the cryoembedding apparatus from pathology innovations? Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated coverslipper with Ventana labels
We have a 15 year old Sakura film coverslipper that has performed famously with Venatana labels. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson [tjohn...@gnf.org] Sent: Monday, October 31, 2011 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated coverslipper with Ventana labels Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
