RE: [Histonet] Question regarding cutting on microtome
Your waterbath is not hot enough! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Spencer Sent: Tuesday, November 29, 2011 10:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question regarding cutting on microtome I am wondering what causes a ribbon appearance in skin when viewed under the microscope, usually seen in the epidermis, but occasionally can be seen in the underlying tissue as well. I find this effect happens only in a few, 4 or 5 slides out of 100, but, I don't know how to correct it. I would describe it as the old fashion hard candy that was rippled like a ribbon could be. Under the scope, focusing up and down demonstrates that the section is complete, just not on the same plane. Any suggestions? Thank you for your time M. Spencer On 11/27/11, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. dako autostainer labeler (Patsy Ruegg) -- Message: 1 Date: Sun, 27 Nov 2011 08:55:58 -0700 From: Patsy Ruegg pru...@ihctech.net Subject: [Histonet] dako autostainer labeler To: histonet@lists.utsouthwestern.edu Message-ID: B29772909A06463CB04824EF6F2F4687@prueggihctechlt Content-Type: text/plain; charset=us-ascii Greetings, I am looking for another Seymour labeler like the one used on the Dako Autostainer, does anyone have one sitting around not being used I could purchase from you? It needs to have the software disc so I can install it on a computer. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 39 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chameleon tissue lifting off
I would like to ask anyone who sections chameleon tissue what type of slides they use. I was having too much lifting while using the superfrost plus slides. It looked like slides coated with Haupt's solution were highly recommended, so I tried that. It is so much better, but still there is some lifting. If someone is successful with sectioning and staining lizard tissue without lifting, please advise me on type of slides, drying times, or anything that might help. Thank you so much, Nancy Thomas Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello All, We are having a issue with our intercom system in the OR so the pathologist are having issues communicating frozen section diagnosis with the surgeon. Does anyone in Histoland use a written form to send frozen section diagnosis? Per CAP if it is verbal the pathologist must speak directly with the surgeon. Any suggestions would be much appreciated. Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
We switched from intercoms to phones some years back. The phone is put on speaker mode in the OR. I suppose problems can occur with the phone system too, but we haven't experienced this. Regarding the CAP part of the question, I would call them. Ask if you can send a written diagnosis, which you are to receive back with the physician's/designee's signature. You can then document the intercom problems, and how you worked around it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jthaw...@shorememorial.org Sent: Wednesday, November 30, 2011 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello All, We are having a issue with our intercom system in the OR so the pathologist are having issues communicating frozen section diagnosis with the surgeon. Does anyone in Histoland use a written form to send frozen section diagnosis? Per CAP if it is verbal the pathologist must speak directly with the surgeon. Any suggestions would be much appreciated. Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immunohistochemical staining of type IV collagen in Alport's Syndrome
We are looking into the feasability of doing this test in-house. Is anyone doing this test for kidney and if so, how did you run your validation? We have some positive patients but they are all skin so I guess we will need to purchase controls specifically for kidney. If anyone has any experience with this I would appreciate your comments. Thanks. Christie Gowan UAB University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part-time Job
Needed a PRN for a small GI Lab in Houston… The position is one week a month from 2-10pm depending on workload. Position includes grossing BX’s, processing, embedding and cutting. If interested in position please email your resume along with any questions. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fresh specimens after hours
for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours? Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7. As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fresh specimens after hours
RonnieFrom my experience when working in labs that are not staffed 24/7 with HT or grossing personnel, after hours fresh specimens are called to a pathologist on call by the OR staff, who may then make the judgment to instruct them on fixation needed, or come in for a FS or view the specimen. Most places have used a pager or page # system with an on call rotation. Sometimes a histology person has been on -call after hours too ( if they usually do the FS, accessioning etc at the bench in lieu of PA) or just be available certain hours if the pathologist needs them to assist with FS, other preparations for send outs etc.I think this is pretty much what you have suggested, and that seems to be in alignment with the arrangments and expectations I have worked for these circumstances in a few of my positions without 24 hr staff.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: ronald.hous...@nationwidechildrens.org To: histonet@lists.utsouthwestern.edu Date: Wed, 30 Nov 2011 17:10:12 + Subject: [Histonet] fresh specimens after hours for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours? Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7. As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASR FISH probes
Hi all, More questions on FISH. How does one go about validating an ASR probe on FFPE tissue? I have no FISH experience and therefore am starting from scratch. The specific probes that our pathologists are interested in are: t(14;18), t(17;22) and t(2;5). Also, does anyone know what educational and training background is required to be able to do such a workup? I know IVDs are no problems, I looked at the CAP checklist for molecular and it seems that this is usually done in cytogenetics and the tech needs to at least have a BS and experience with molecular. Any help would be greatly appreciated! Thanks, Erin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Position Available
UT MD Anderson Cancer Center in Houston, Texas has a vacant position for a Clinical Histology Technician The candidate should have experience sectioning paraffin embedded biopsies. The shift for this position is either 11:00 pm - 7:30 am or 10:30 pm - 6:30 am Sunday through Thursday. Apply online at www.mdanderson.orghttp://www.mdanderson.org or contact the recruiter Nikol Blackmon at 713-745-6349. Kaye Barr, HT(ASCP) Laboratory Manager, PLM Pathology Dept. UT M.D. Anderson Cancer Center Houston, Texas 713-792-5366 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fresh specimens after hours
After Surgical Pathology closes, consider having all specimens taken to the clinical lab (staffed 24/7) and have them check in all specimens. If a FS is needed, they can contact the on-call pathologists and tech. If no FS, they can have instructions, by tissue type, to add the correct fixative or just refrigerate. Surgical Path staff picks up specimens from the clin lab the next morning. William DeSalvo, B.S., HTL(ASCP) From: ronald.hous...@nationwidechildrens.org To: histonet@lists.utsouthwestern.edu Date: Wed, 30 Nov 2011 17:10:12 + Subject: [Histonet] fresh specimens after hours for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours? Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7. As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Jobs in Janesville, WI
All, Mercy Hospital and Trauma Center in Janesville, WI has openings for a full-time and a pool histotechnician. Please visit www.mercyhealthsystem.org for more details. Thank-you, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Mercy Hospital and Trauma Center Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Subject: [Histonet] nuclear fast red
Nuclear Fast Red needs to be heated during prep and it does take a little while to dissolve, it never really dissolves entirely, probably saturates the solution pretty fast. Tim Message: 1 Date: Tue, 29 Nov 2011 11:29:07 -0700 From: Patsy Ruegg pru...@ihctech.net Subject: [Histonet] nuclear fast red To: histonet@lists.utsouthwestern.edu Message-ID: 94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice Content-Type: text/plain; charset=us-ascii Is my nuclear fast red powder dead or does it take a long time with heat and stirring to turn red? It is pretty old, and one vial says it should be stored below 0 but has not been. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email mailto:pru...@ihctech.net pru...@ihctech.net web site http://www.ihctech.net www.ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASCP membership
Hello all, I was wondering if the ASCP membership is worth enrolling, especially since it is a little pricey but it can also keep track with regards too CMP's. Your input would be very beneficial. Thank you ahead for your time. Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongo...@ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MAP-2 Troubleshooting
Hi Histonet- I am posting this question for a colleague of mine who is having some problems performing a MAP-2 stain. Problem: Blotchy or wormy areas of light or absent staining of MAP-2 (Thermo Scientific MS-250-R7) in brain sections by immunohistochemistry. Microwave antigen retrieval was performed using 10 mM citric acid at pH 6. The stain worked very well historically. Charged slides were stained by immersion or individually using PAP barrier. The areas of stain loss are repetitive on neighboring slides, which suggests antigen retrieval as the problem step. Several attempts at modifying the retrieval process have not been unsuccessful. Has anyone else been running into these issues? Any advice or tips would be greatly appreciated! Thank you. Erin Sarricks, HT (ASCP) Histology Team Leader USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil Phone: 410-436-1967 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ASCP membership
I do not think it is worth enrolling, at least from the histology subject point of view. Check the contents of several issue and you will realize that articles about histology are very few, I think that less 1 in 25, if that many. I had subscribed and unsubscribed at least twice. René J. --- On Wed, 11/30/11, Diana Martinez-Longoria dmlongo...@ecrmc.org wrote: From: Diana Martinez-Longoria dmlongo...@ecrmc.org Subject: [Histonet] ASCP membership To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, November 30, 2011, 3:08 PM Hello all, I was wondering if the ASCP membership is worth enrolling, especially since it is a little pricey but it can also keep track with regards too CMP's. Your input would be very beneficial. Thank you ahead for your time. Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongo...@ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: fresh specimens after hours
The Pathologist on call handles these calls. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, November 30, 2011 12:10 PM To: 'histo net' Subject: [Histonet] fresh specimens after hours for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours? Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7. As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Case Distribution
Can anyone provide me with inforrmation regarding how you distribute cases to pathologists. I work for a region which has cases coming in from many sites and pathologists reading cases from three sites. Our caseload is approx 35,000 cases per year of varying specimen types. We are looking at changing how we distribute cases right now I am just fishing for ideas to make sure that we consider different ideas and hopefully come up with a process that meets our needs. Thanks Sharon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 96, Issue 42
@15
Wrap tissue in moist, not too wet!!, (0.9% salt or PBS) gauze and store
at 4 degrees C. No/hardly any harm done to morphology, antigenicity, RNA,
DNA and so on. Tissue is tougher than you think...
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027704
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...
Today's Topics:
1. nuclear fast red (Patsy Ruegg)
2. RE: nuclear fast red (Elizabeth Chlipala)
3. Histologist needed in Austin Texas (Esparza, Sandra)
4. validation (Amos Brooks)
5. autostainer vials (Thomas Pier)
6. Diane Tokugawa/CA/KAIPERM is out of the office.
(diane.tokug...@kp.org)
7. Question regarding cutting on microtome (Marcia Spencer)
8. RE: Question regarding cutting on microtome (Britton, Josette C)
9. Chameleon tissue lifting off (Thomas, Nancy)
10. (no subject) (jthaw...@shorememorial.org)
11. RE: (no subject) (Rathborne, Toni)
12. RE: Chameleon tissue lifting off (WILLIAM DESALVO)
13. Immunohistochemical staining of type IV collagen in Alport's
Syndrome (CHRISTIE GOWAN)
14. Part-time Job (Maria T)
15. fresh specimens after hours (Houston, Ronald)
16. RE: fresh specimens after hours (joelle weaver)
17. ASR FISH probes (Martin, Erin)
--
Message: 1
Date: Tue, 29 Nov 2011 11:29:07 -0700
From: Patsy Ruegg pru...@ihctech.net
Subject: [Histonet] nuclear fast red
To: histonet@lists.utsouthwestern.edu
Message-ID: 94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice
Content-Type: text/plain; charset=us-ascii
Is my nuclear fast red powder dead or does it take a long time with heat
and
stirring to turn red? It is pretty old, and one vial says it should be
stored below 0 but has not been.
Cheers,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that
is
privileged confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited.
If
you are NOT the intended recipient please contact the sender and dispose
of
this e-mail as soon as possible.
--
Message: 2
Date: Tue, 29 Nov 2011 13:22:21 -0700
From: Elizabeth Chlipala l...@premierlab.com
Subject: RE: [Histonet] nuclear fast red
To: Patsy Ruegg pru...@ihctech.net,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
14E2C6176416974295479C64A11CB9AE011380AD9C8D@SBS2K8.premierlab.local
Content-Type: text/plain; charset=us-ascii
Patsy
It does not go completely in solution we filter prior to staining
Liz
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
[pru...@ihctech.net]
Sent: Tuesday, November 29, 2011 11:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] nuclear fast red
Is my nuclear fast red powder dead or does it take a long time with heat
and
stirring to turn red? It is pretty old, and one vial says it should be
stored below 0 but has not been.
Cheers,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that
is
privileged confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or
[Histonet] RE: ASCP membership
I think it is benefital, I believe you have access to 3 CEU credits each year when you join. I would also look at the Remember program too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana Martinez-Longoria Sent: Wednesday, November 30, 2011 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP membership Hello all, I was wondering if the ASCP membership is worth enrolling, especially since it is a little pricey but it can also keep track with regards too CMP's. Your input would be very beneficial. Thank you ahead for your time. Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongo...@ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: EM questions
Can anyone help with this, I haven't got a clue?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-Original Message-
From: Suarez, Andrea Luisa [mailto:andrea.sua...@ucdenver.edu]
Sent: Wednesday, November 30, 2011 3:22 PM
To: pru...@ihctech.net
Cc: High, Whitney
Subject: EM questions
Hi Patsy,
Not sure if you remember me from my days as a graduate student in the van
Dyk lab. How are you?
I am working w/ Dr Whit High at CU dermpath on an EM project. I had some
questions that perhaps you might have some answers to. We are doing a
project where we will be doing QEMSCAN at school of mines, which has the
capacity to do a very sophisticated analysis of mineral composition. We
will be looking for volcanic ash deposition in human skin samples, and would
like to embedd the tissues in as pure a fashion as possible, with optimum
antigen preservation. We have some concerns about parrafin embedding, as we
plan to mount the sections onto carbon planchets. Our experience w/ this in
the past, is that adherence to the planchet is poor after deparafinizaiton.
Therefore, we would like to optimize adherence, if possible, or avoid the
need for deparafinization by embedding in a wax (such as carnuba) w/ a very
high melting temp.
1.) Do you have any experience/insight regarding embedding in carnuba wax?
Do you know of anyone who would be willing to do this for us?
2.) Do you have any recomendations as to how to improve adherence to the
carbon planchet in the event that we do end up having to de-parafinize? We
were thinking that adding albumin to the water bath may help things. Any
thoughts on this?
Many thanks and I look forward to hearing from you.
Sincerely,
Andrea Suarez, MD/PhD
Colorado Health Foundation Transitional Intern
andrea.sua...@ucdenver.edu
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Info for all Re: nuclear fast red
Nuclear fast red was added to the Biological Stain Commission's list of Certified Stains 4 or 5 years ago. http://biostain.com Buy from a vendor who sells NFR from a Certified batch. Think before buying anything else. John Kiernan Secretary, Biological Stain Commission = = = On 30/11/11, Tim Higgins thigg...@cddmedical.com wrote: Nuclear Fast Red needs to be heated during prep and it does take a little while to dissolve, it never really dissolves entirely, probably saturates the solution pretty fast. Tim Message: 1 Date: Tue, 29 Nov 2011 11:29:07 -0700 From: Patsy Ruegg pru...@ihctech.net Subject: [Histonet] nuclear fast red To: histonet@lists.utsouthwestern.edu Message-ID: 94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice Content-Type: text/plain; charset=us-ascii Is my nuclear fast red powder dead or does it take a long time with heat and stirring to turn red? It is pretty old, and one vial says it should be stored below 0 but has not been. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email mailto:pru...@ihctech.net pru...@ihctech.net pru...@ihctech.net web site http://www.ihctech.net(http://www.ihctech.net/) www.ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
