RE: [Histonet] medi tech users
We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] medi tech users
We order ours in lab. Dawn D. Schneider Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 12, 2012 5:09 AM To: 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] medi tech users We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] medi tech users
We order ours in Pathology also. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, January 12, 2012 10:25 AM To: 'Tom McNemar'; 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] medi tech users We order ours in lab. Dawn D. Schneider Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 12, 2012 5:09 AM To: 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] medi tech users We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechs needed in NY and CT Can you help?
Hi Histonetters!! I hope you are having a great day. I have several new positions and I need your help. I am presently on a search for several of my best clients that are in need of Histo Techs all of my clients are private labs and are located in CT and NY. My clients offer excellent compensation, benefits and environments that are great to work in. These are permanent full time position. NY License required in NY and ASCP certification is required in CT Here are the positions: Histology Lab Supervisor Suffern, NY Histology Tech Uniondale, NY Histotech Rochester, NY Grossing Histotech Wallingford, CT The help I need is do you know anyone that might be interested in hearing about either this opportunity? If so could you please forward my e-mail to them? If you are interested in the histo tech position please call me ASAP at 866-607-3542 or e-mail me at rel...@earthlink.net Thank you, Pam 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pms 2 antibody trouble
I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but nothing is consistent. We are looking to try another vendor's antibodies, but were hoping for some feedback as to which labs have found success in this area. I have never had trouble like this with an antibody. The severe problem here is that with MSI testing, we are looking for lack of staining with this antibody to show a positive result so light patchy staining leaves a lot of room for error. Any responses are appreciated. Thank you, Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.free...@utoledo.edu (419)383-5639 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] autopsy tables
Hi All, Anyone know of a company that repairs autopsy tables? Our engineering dept is trying to fix the hydrolics and the grinder, but can't seem to get it to work. Of course, we had an autopsy scheduled yesterday! We would also be interested in a refurbished model if any vendors are out there. Thanks, Michele M. Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Multiple IHC on Sentinel Nodes
Good Morning to All, We do cytokeratin IHC on all blocks from the sentinel node specimen. Sometimes we have had up to 12 blocks from the sentinel node specimen. SO, given the previous information on Histonet, can we bill 88342 x 12 on a sentinel node when all blocks from that specimen are being stained. Or is it 88342 x 1??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Multiple IHC on Sentinel Nodes
88342 x 1 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Thursday, January 12, 2012 11:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Multiple IHC on Sentinel Nodes Good Morning to All, We do cytokeratin IHC on all blocks from the sentinel node specimen. Sometimes we have had up to 12 blocks from the sentinel node specimen. SO, given the previous information on Histonet, can we bill 88342 x 12 on a sentinel node when all blocks from that specimen are being stained. Or is it 88342 x 1??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] out of office
I will be out of the office starting 01/12/2012 and will not return until 01/16/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin Neutralizing
Very good point Rene! Formalex now comes with a test kit for unreacted aldehydes. I used to run the simple test with every batch...sometimes, I had to add extra solution. http://www.dtsc.ca.gov/TechnologyDevelopment/TechCert/american-biosafety-formalex-techadv.cfm Tracy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 11, 2012 2:06 PM To: 'histonet@lists.utsouthwestern.edu'; Amy Self Subject: Re: [Histonet] Formalin Neutralizing Amy: During the years I had the same dream as yours. Because of that I did try Transfor-121, Vytak, Formalex, Aldex-AMS 140, Neutralex and a 1% solution of potasium permanganate. To test the effectiveness of the neutralization of formalin I used a residual reaction to PAS (Schiff) and ALL the results were positive to PAS, meaning that the neutralization was incomplete. I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with the same incomplete results. Those results prevented me to try to dispose of the neutralized formalin and I kept using my disposal company in spite of the costs. I could not convince myself to dispose of formalin neutralized with the products I mention. René J. --- On Wed, 1/11/12, Amy Self as...@georgetownhospitalsystem.org wrote: From: Amy Self as...@georgetownhospitalsystem.org Subject: [Histonet] Formalin Neutralizing To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Wednesday, January 11, 2012, 10:50 AM Hello Histonetters, I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Openings Nationwide
Prometheus Healthcare, the nation's # 1 Recruiter for Laboratory professionals, is currently seeking technician and technologists for numerous histology opportunities across the country. Shifts vary from day, evening to night and opportunities are available within grossing, embedding, cutting, staining, and IHC. Supervisor positions are also available. The facilities we work with include some of the most prestigious hospitals, reference labs and biotech companies. Please contact us today at 301-693-9057 to discuss our current openings. Requirements: ASCP preferred At least 1 year histology experience Steven Rdell Wagner National Account Manager Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 ste...@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Open FL Histology Position!!
I am currently working on a daytime histology position is in SE Florida with a top hospital. A beautiful and exciting work environment with a great team focused department. For more information, please feel free to reach me at 301.693.9057 or ste...@prometheushealthcare.com Steven Wagner National Account Manager Prometheus Healthcare Office 301-693-9057 Cell 301-698-8908 Fax 301-368-2478 ste...@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pms 2 antibody trouble
We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, Freeman, Carol carol.free...@utoledo.edu wrote: I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but nothing is consistent. We are looking to try another vendor's antibodies, but were hoping for some feedback as to which labs have found success in this area. I have never had trouble like this with an antibody. The severe problem here is that with MSI testing, we are looking for lack of staining with this antibody to show a positive result so light patchy staining leaves a lot of room for error. Any responses are appreciated. Thank you, Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.free...@utoledo.edu (419)383-5639 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pms 2 antibody trouble
I just bought the MSI panel antibodies (MLH-1, MSH2, MSH6 and PMS2) from Ventana/Cell Marque and I'm running them on the Ultra and XT: and I'm having trouble, as well, getting them to the desired staining for my pathologists. I'm staining them at CC1 standard and antibody incubation time is 40 min and I use the amplifier except the MSH2 which is the only one that has been signed off on at 32 min incubation time, no amplifier and mild CC1. What protocol are you using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Karen Lahti Sent: Thursday, January 12, 2012 3:05 PM To: Freeman, Carol Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pms 2 antibody trouble We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, Freeman, Carol carol.free...@utoledo.edu wrote: I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but nothing is consistent. We are looking to try another vendor's antibodies, but were hoping for some feedback as to which labs have found success in this area. I have never had trouble like this with an antibody. The severe problem here is that with MSI testing, we are looking for lack of staining with this antibody to show a positive result so light patchy staining leaves a lot of room for error. Any responses are appreciated. Thank you, Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.free...@utoledo.edu (419)383-5639 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin Neutralizing
Hi, To take it a step further, if you look at the MSDS of the Neutralex, it is very nebulous as to what the chemical is. We couldn't dump the Neutralex alone down the sink since we don't know what it is. So combining a hazardous chemical with an unknown chemical then dumping it down the sink... I think that would be enough to have any safety officer up in arms. Amos On Thu, Jan 12, 2012 at 10:41 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 2 Date: Wed, 11 Jan 2012 13:05:51 -0800 (PST) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Formalin Neutralizing To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu,Amy Self as...@georgetownhospitalsystem.org Message-ID: 1326315951.50865.yahoomailclas...@web65702.mail.ac4.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Amy: During the years I had the same dream as yours. Because of that I did try Transfor-121, Vytak, Formalex, Aldex-AMS 140, Neutralex and a 1% solution of potasium permanganate. To test the effectiveness of the neutralization of formalin I used a residual reaction to PAS (Schiff) and ALL the results were positive to PAS, meaning that the neutralization was incomplete. I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with the same incomplete results. Those results prevented me to try to dispose of the neutralized formalin and I kept using my disposal company in spite of the costs. I could not convince myself to dispose of formalin neutralized with the products I mention. René J. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin Neutralizing
Indeed! That is why I always advocate for safety. Safety for the personnel and the environment. Sometimes saving some money by neutralizing something is neither safe nor economic. Falling to a sales pitch is always unwise. René J. --- On Thu, 1/12/12, Amos Brooks amosbro...@gmail.com wrote: From: Amos Brooks amosbro...@gmail.com Subject: [Histonet] Formalin Neutralizing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 12, 2012, 4:38 PM Hi, To take it a step further, if you look at the MSDS of the Neutralex, it is very nebulous as to what the chemical is. We couldn't dump the Neutralex alone down the sink since we don't know what it is. So combining a hazardous chemical with an unknown chemical then dumping it down the sink... I think that would be enough to have any safety officer up in arms. Amos On Thu, Jan 12, 2012 at 10:41 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 2 Date: Wed, 11 Jan 2012 13:05:51 -0800 (PST) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Formalin Neutralizing To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu, Amy Self as...@georgetownhospitalsystem.org Message-ID: 1326315951.50865.yahoomailclas...@web65702.mail.ac4.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Amy: During the years I had the same dream as yours. Because of that I did try Transfor-121, Vytak, Formalex, Aldex-AMS 140, Neutralex and a 1% solution of potasium permanganate. To test the effectiveness of the neutralization of formalin I used a residual reaction to PAS (Schiff) and ALL the results were positive to PAS, meaning that the neutralization was incomplete. I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with the same incomplete results. Those results prevented me to try to dispose of the neutralized formalin and I kept using my disposal company in spite of the costs. I could not convince myself to dispose of formalin neutralized with the products I mention. René J. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin
I was just wondering what Paraffin most people think is the best for all around embedding, including bone samples. What cuts better, stretches better, etc. Thanks, nancy.lo...@med.va.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trimming question
Hello Histonetters, I'm wondering what solution you put your tissues/cassettes in while trimming fixed tissues. Formalin? 70%? Water? Something else? Thanks, Kate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nail Polish sealant
Amen Gayle, as always you hit the nail on the head. I never use nail polish sealant because of being burned so many times due to the isopropanol leaching into - and ruining - the sample. In addition if it gets onto pricey fluorescence lenses it's difficult to clean. It's not worth the risk, especially given the great hard set mountants available. I also detest the mountants with DAPI mixed in. Nothing better tan doing your own even counter stain with Hoechst 33342 for 10 minutes before mounting for crisp beautiful nuclei. Andrea On Jan 5, 2012, at 12:35 PM, gayle callis gayle.cal...@bresnan.net wrote: You wrote: We are currently starting up some IHC on frozen tissue sections. After staining with different fluorescent antibodies, we end with applying DAPI w/Prolong gold and then coverslipping. We would like to seal the coverslip so that we can keep the slides longer. Any suggestions on where and how best to apply the nail polish for a permanent fix on the coverslips? * Prolong Gold antifade reagent is a hard seal to begin with. We only seal ends but not the sides of cover glass. Our coverslips go right to edge of slides e.g. 25 X 30, 25 x 40, or 25 X 50.We don't like having nail polish slop over the edges onto back of slides but one could seal all sides of coverslip if careful. We buy the thinner top or base coat nail polish, but in general, prefer to use thinned mounting media rather than nail polish. There can be some issues here.If you are trying to view GFP or RFP (red fluorescence protein) labeled cells or tissue components, you should not seal the coverslip with nail polish since the alcohol in nail polish leaches under the coverslip and causes GFP/RFP to fade. This fact was published in Science. We found that dumping out cheap clear nail polish from bottle, rinsing away the residue with acetone, and then adding permanent mounting media and thinning that with toluene to the consistency of top coat nail polish works best. Toluene or xylene based sealants cannot leach under the cover glass since these solvents are NOT miscible with water in the PBS.Thinned mounting media is better sealant for GFP purposes (no fading) and also works for IF stained sections (perfect seal). We love the little brush in the nail polish bottle for application. Thicker clear nail polish (for non GFP studies) or IF stained sections is messy during application so we buy the cheapest top coat polish we can find at Walmart. DAPI in the Prolong Gold will cause an uneven staining gradient so that some of the nuclei in the center of a section are not stained as brightly as the nuclei on the outer edges of a stained section. The cause is not getting enough thicker Prolong Gold/DAPI over the section or not having just the right amount of buffer on the section to permit a good flow of this wonderful mounting media over the section.We now complete all IF staining then stain with a DAPI solution before cover slipping with Prolong Gold. You can buy ready to use DAPI solutions from Pierce or Biogenex, or make up the solution in house. You can find the recipe at IHCworld website or simply Google. We do NOT store our IF stained slides in the cold, but in a folder at RT in a dark drawer before viewing on the day after staining to reduce any movement/flow under the coverslip. Fluorophores can and will eventually fade. I do not recall any studies saying storing IF stained slides in the cold reduces fading but we never have space to do cold storage anyway and store slides at RT.The new fluorophores (Alexas and Dylights) remain stable over a longer time even for several weeks compared to fluorescein derivatives e.g. FITC TRITC. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 98, Issue 17
Carol, Are you doing the MSI by hand, or by autostainers (which platform?). Here's my protocols that I have optimized; Protocol A for Dako Plus (or manually by hand); 1) AR; Biocare's Diva with Pascal pressure cooker for 3-5 min 2) Quench End. Peroxidase; Dako's End.H202 for 5 min 3) Incubate 1st Ab; PMS2 (Cell Marque; RTU) antibody for 60 min 4) Incubate 2nd Ab; Dako Envision +/HRP for 30 min 5) Develop color; Dako Envision DAB+ for 5 min 6) Counterstain; Mayer Hematoxylin for 2 min 7) Dehydrate cover slip with permanent mounting Protocol B for Leica Bond; 1) Use Protocol F with Bond Refine detection kit 2) HIER; ER2 for 20 min My IHC staining are always strong and consistent (manually by hand, Dako Plus, or Leica Bond). Hope this help! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX Histology) El Camino Hospital madelein...@elcaminohospital.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
