[Histonet] EGFR ISH protocol for Ventana
--Original message Hi Can someone help me with the EGFR ISH protocol for Ventana? Thanks! Mei Zheng, HTL(ASCP) QIHC Clinical Supervisor Immunohistochemistry Lab. Department of Pathology Brigham and Women's Hospital Boston, MA 02115 Lab Tel: 617 732-7790 --Reply Hi Mei, Sorry for the delay. Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the Ventana Benchmark XT. First, the software that you will need to have installed is called XT Dual Color Open Probe. This protocol will use the UltraView Silver ISH DNP Detection for EGFR and UltraView Red ISH DIG Detection for Chromosome 7. The counterstain is Hematoxylin II with Bluing Reagent. Before I get to the protocol, I'll fill you in on the EGFR and Chromosome 7 probes. They do NOT arrive in a dispenser. They each arrive in pre-diluted vials, so you will need to fill a user-fillable dispenser. The pre-diluted vials are mixed together into one user-fillable dispenser to create a cocktail. Don't forget you will also need a button kit for ISH PROBE because the user-fillable dispenser will not have the proper commercial bar code. One more thing, UltraView Red ISH DIG Detection cannot be dehydrated in alcohol. I usually dry the slides at 65C for an hour before I coverslip. Finally, the following is a successful protocol summary. 1 Deparaffinization [Selected] 2 Extended [Selected] 3 Warmup Slide to [72 Deg C], and Incubate for 4 Minutes ( Deparaffinization ) 4 Cell Conditioning [Selected] 5 CC2 [Selected] 6 CC2 Cycle 1 [Selected] 7 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Cycle 1 ) 8 CC2 Cycle 2 [Selected] 9 Incubate for [12 Minutes] ( Cycle 2 ) 10 CC2 Cycle 3 [Selected] 11 Incubate for [12 Minutes] ( Cycle 3 ) 12 Enzyme [Selected] 13 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), Apply Coverslip, and Incubate for [32 Minutes] 14 Probe Auto Dispense [Selected] 15 Apply One Drop of [ISH Probe 3] ( ISH Probe ), No Coverslip and Incubate for 8 Minutes 16 Denature [Selected] 17 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Denaturation ) 18 Hybridization [Selected] 19 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 20 Incubate for [6 Hours] ( Hybridization ) 21 Stringency Washes [Selected] 22 Stringency Wash #1 [Selected] 23 Warmup Slide to [72 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 24 Stringency Wash #2 [Selected] 25 Incubate for [8 Minutes] ( Stringency Wash #2 ) 26 Stringency Wash #3 [Selected] 27 Incubate for [8 Minutes] ( Stringency Wash #3 ) 28 Silver Detection [Selected] 29 Apply One Drop of SIL ISH DNP RAB, Apply Coverslip, and Incubate for [20 Minutes] 30 Apply One Drop of SIL ISH DNP CHRC, and Incubate for [4 Minutes] 31 Red Detection [Selected] 32 Apply One Drop of RED ISH DIG MAB, Apply Coverslip, and Incubate for [20 Minutes] 33 Apply One Drop of RED ISH DIG FR, and Incubate for [4 Minutes] 34 Counterstain [Selected] 35 Apply One Drop of [HEMATOXYLIN II] ( Counterstain ), Apply Coverslip, and Incubate for [8 Minutes] 36 Post Counterstain [Selected] 37 Apply One Drop of [BLUING REAGENT] ( Post Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 38 Post Run LCS Application [Selected] Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 === Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Histology Positions - Northern IL - Retained Search
Good Morning, Personify has had 2 new permanent Histology opportunities become available in the Northern IL area. The positions offer competitive packages and the opportunity for career growth. Please contact me directly at mw@personifysearch to learn more. *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacture and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunities:* (1)QA Histologist: The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. (2)Histologist: The company currently has an opening for a Histologist working on the RD team to be based in Richmond IL. Salary: Based on Experience Other: Full benefits - 401k program/matching *Education and Experience Required:* - Experience with tissue grossing, tissue processing and embedding - Experience with sectioning paraffin embedded tissue as well as frozen tissue - Experience performing routine stains (H and E) as well as special stains - Experience with formulation and production of routine laboratory reagents and solutions - Experience performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Proficiency in basic computer skills and with software applications such as Microsoft Office Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flammable / explosion proof refrigerator
What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor? I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or rule to help justify getting an equipped refrigerator for flammable refrigerated reagents. Please let me know some facts! Thanks Angela ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flammable / explosion proof refrigerator
Angela, the only requirement I know of is your need to store solvents that could ignite if the thermostat switch sparks. We have one for special stains. We also have one that has one bottle of acetone in it. The vapor from that could ignite. They aren't that much more expensive than a regular refrigerator. They just put the thermostat switch on the outside rather than the inside. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Thursday, February 09, 2012 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable / explosion proof refrigerator What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor? I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or rule to help justify getting an equipped refrigerator for flammable refrigerated reagents. Please let me know some facts! Thanks Angela ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NCCI policy changes on IHC billing
My apologies, the document is too large for Histonet. If anyone needs to see it, email me directly. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Mandy Sent: Thursday, February 09, 2012 12:43 PM To: Histonet Subject: [Histonet] NCCI policy changes on IHC billing Histonetters, I had forwarded the thread to our pathologists regarding the changes to IHC billing policies. They could not find any documentation about any changes to billing for cocktailed antibodies and/or dual stains. We looked at the NCCI website and could not find any information referencing this. Does anyone know where I could find this information? Thanks, Mandy Bell HTL(ASCP) Community Hospital of the Monterey Peninsula 2 Harris Court Suite B3/B4 Monterey, CA 93940 (831) 647-4791 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet QIHC
Hi Sarah, I've been working on editing a QIHC Study Guide (sorry, it's not ready for the public yet!) and so I've been paying quite a bit of attention to what is on the QIHC exam now-a-days. I've found that most of the QIHC questions are complex analysis and problem solving type questions. It's not enough to know that facts, you must understand how to use the info you have. Since you have been doing hand-staining and troubleshooting, I expect you will have a good handle on that stuff. I recommend that you get the DAKO Immunohistochemical Staining Methods Educational Guide and Educational Guide to Demasking Antigens (both are free from DAKO, just call them!) and review those. The Dako books provide the most concise, complete info you can find. Review the basic immunology stuff for a refresher. Pay attention to all the various systems for IHC. Pay extra attention to antigen retrieval, there are quite a few questions relating to that. Also, understand how to do the calculations for dilutions and titrations. You can expect a couple oddball questions about IHC on cytology preps and frozen sections. Good Luck! Beth Cox, HTL/SCT(ASCP)QIHC ___ Message: 6 Date: Thu, 9 Feb 2012 15:35:29 + From: Sarah Dysartsdys...@mirnarx.com Subject: [Histonet] QIHC To: histonethistonet@lists.utsouthwestern.edu Message-ID: 8a70a9b2ecdd084dacfe6c59fcf86d5001cfc...@bl2prd0710mb363.namprd07.prod.outlook.com Content-Type: text/plain; charset=us-ascii Hey ya'll. I have been doing IHC for 10 years or so now. Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying. I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself. If you do think something would be worth going over what would you suggest? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 99, Issue 11
Hi Lucie, We use both rack systems in our Revcos but find the front sliding access are really useful and has significantly increased our freezer box organizationno sliding-sticking problems. My only issue is these racks come in a huge frame that occupies the complete shelf space.leaving no space for non-boxed stuff (bottles, specimen bags, etc). So, we have dedicated two shelves to these front sliding racks and other two have the side racks and oddball stuff. Hope this helps. Praveen Praveen Arany, HSEAS, Cambridge. On 2/9/2012 1:03 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Hi all, We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2 box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cryostats decontamination
Seems like overkill to us, too, especially since we have to scrub ours down between each patient (we are sectioning tissue that goes to Molecular for DNA/RNA isolation after us) and had only been taking them completely down quarterly, never having more than 2 of our 5 units down at any time. Our cryostats look showroom new even in mid-shift. Now, we'll take them down on Friday afternoon, clean them on Monday morning and then wait for them to cool down enough to be able to work. Barb Newton Morphology Core Lab/TCGA Project Nationwide Children's Hospital 614-355-2915 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, February 09, 2012 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 99, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Temporary Tech Agencies (Jennifer MacDonald) 2. cryostats decontamination (anita dudley) 3. Revco -80 Freezer Sliding Drawer Rack Reviews (Lucie Guernsey) 4. EGFR ISH protocol for Ventana (Lanigan, Christopher) 5. New Histology Positions - Northern IL - Retained Search (Matt Ward) 6. QIHC (Sarah Dysart) 7. RE: QIHC (Walter Benton) 8. exit strategy for staff (Edwards, Richard E.) 9. (no subject) (Freeman, Carol) 10. Flammable / explosion proof refrigerator (angela smith) 11. RE: Flammable / explosion proof refrigerator (Morken, Timothy) -- Message: 1 Date: Wed, 8 Feb 2012 09:40:10 -0800 From: Jennifer MacDonald jmacdon...@mtsac.edu Subject: [Histonet] Temporary Tech Agencies To: histonet@lists.utsouthwestern.edu Message-ID: of17bac925.14142466-on8825799e.0060f27d-8825799e.00611...@mtsac.edu Content-Type: text/plain; charset=US-ASCII Hi All, I have a lab that is in need of temporary help for a maternity leave. Does anyone know of a good temporary agency? Thanks, Jennifer MacDonald -- Message: 2 Date: Wed, 8 Feb 2012 16:49:29 -0600 From: anita dudley azdud...@hotmail.com Subject: [Histonet] cryostats decontamination To: histonet@lists.utsouthwestern.edu Message-ID: blu163-w55b7dea6e8729d2249fc95d1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley -- Message: 3 Date: Wed, 8 Feb 2012 15:07:09 -0800 From: Lucie Guernsey lguern...@ucsd.edu Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews To: histonet@lists.utsouthwestern.edu Message-ID: canqoiz3d1wvb-av5orfcj2ke8mraabdtnuzuaxgi8l5_1dm...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi all, We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2 box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu -- Message: 4 Date: Thu, 9 Feb 2012 07:02:05 -0500 From: Lanigan, Christopher lani...@ccf.org Subject: [Histonet] EGFR ISH protocol for Ventana To: histonet@lists.utsouthwestern.edu Message-ID: 9ddd0f026dc71b41b79d8e439d7951a30bab4...@cchsclexmb69.cc.ad.cchs.net Content-Type: text/plain; charset=us-ascii --Original message Hi Can someone help me with the EGFR ISH protocol for Ventana? Thanks! Mei Zheng, HTL(ASCP) QIHC Clinical Supervisor Immunohistochemistry Lab. Department of Pathology Brigham and Women's Hospital Boston, MA 02115 Lab Tel: 617 732-7790 --Reply Hi Mei, Sorry for the delay. Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the Ventana Benchmark XT. First, the
RE: [Histonet] PA area folks--company to NOT use
Surely, as healthcare professionals, we can derive a better example of American Freedom than obesity and gluttony. Am I the only one offended by Mr. Swanson's quote being used in a forum for medical professionals? Just curious. Sent from my Windows Phone From: Rene J Buesa Sent: 2/4/2012 9:12 AM To: histonet@lists.utsouthwestern.edu; Emily Sours Subject: Re: [Histonet] PA area folks--company to NOT use You should also file a complaint with the Better Business Bureau of your area. René J. --- On Fri, 2/3/12, Emily Sours talulahg...@gmail.com wrote: From: Emily Sours talulahg...@gmail.com Subject: [Histonet] PA area folks--company to NOT use To: histonet@lists.utsouthwestern.edu Date: Friday, February 3, 2012, 4:12 PM Hello histonetters! I am having a great deal of trouble getting a microscope cleaning bill paid. We were overcharged by an hour. I suggest if you ever need your instruments cleaned or repaired, DO NOT USE GEORGE NABLE INSTRUMENTS. He has consistently overcharged us for his work, even after writing down a certain price on a purchase order. Just a warning. He works in the Pittsburgh area. I wish there was a yelp for scientists where I could post this, because I know a lot of people use him. Emily Sours The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] reply to message 6
check .cqpath.com : all you wanted to know about IHC but were afraid to ask ;-) Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Temporary Tech Agencies (Jennifer MacDonald) 2. cryostats decontamination (anita dudley) 3. Revco -80 Freezer Sliding Drawer Rack Reviews (Lucie Guernsey) 4. EGFR ISH protocol for Ventana (Lanigan, Christopher) 5. New Histology Positions - Northern IL - Retained Search (Matt Ward) 6. QIHC (Sarah Dysart) 7. RE: QIHC (Walter Benton) 8. exit strategy for staff (Edwards, Richard E.) 9. (no subject) (Freeman, Carol) 10. Flammable / explosion proof refrigerator (angela smith) 11. RE: Flammable / explosion proof refrigerator (Morken, Timothy) -- Message: 1 Date: Wed, 8 Feb 2012 09:40:10 -0800 From: Jennifer MacDonald jmacdon...@mtsac.edu Subject: [Histonet] Temporary Tech Agencies To: histonet@lists.utsouthwestern.edu Message-ID: of17bac925.14142466-on8825799e.0060f27d-8825799e.00611...@mtsac.edu Content-Type: text/plain; charset=US-ASCII Hi All, I have a lab that is in need of temporary help for a maternity leave. Does anyone know of a good temporary agency? Thanks, Jennifer MacDonald -- Message: 2 Date: Wed, 8 Feb 2012 16:49:29 -0600 From: anita dudley azdud...@hotmail.com Subject: [Histonet] cryostats decontamination To: histonet@lists.utsouthwestern.edu Message-ID: blu163-w55b7dea6e8729d2249fc95d1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley -- Message: 3 Date: Wed, 8 Feb 2012 15:07:09 -0800 From: Lucie Guernsey lguern...@ucsd.edu Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews To: histonet@lists.utsouthwestern.edu Message-ID: canqoiz3d1wvb-av5orfcj2ke8mraabdtnuzuaxgi8l5_1dm...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi all, We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2 box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu -- Message: 4 Date: Thu, 9 Feb 2012 07:02:05 -0500 From: Lanigan, Christopher lani...@ccf.org Subject: [Histonet] EGFR ISH protocol for Ventana To: histonet@lists.utsouthwestern.edu Message-ID: 9ddd0f026dc71b41b79d8e439d7951a30bab4...@cchsclexmb69.cc.ad.cchs.net Content-Type: text/plain; charset=us-ascii --Original message Hi Can someone help me with the EGFR ISH protocol for Ventana? Thanks! Mei Zheng, HTL(ASCP) QIHC Clinical Supervisor Immunohistochemistry Lab. Department of Pathology Brigham and Women's Hospital Boston, MA 02115 Lab Tel: 617 732-7790 --Reply Hi Mei, Sorry for the delay. Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the Ventana Benchmark XT. First, the software that you will need to have installed is called XT Dual Color Open Probe. This protocol will use the UltraView Silver ISH DNP Detection for EGFR and UltraView Red ISH DIG Detection for Chromosome 7. The counterstain is Hematoxylin II with Bluing Reagent. Before I get to the protocol, I'll fill you in on the EGFR and Chromosome 7 probes. They do NOT arrive in a dispenser. They each arrive in pre-diluted vials, so you will need to fill a user-fillable dispenser. The pre-diluted vials are mixed together into one user-fillable dispenser to create a cocktail. Don't forget you will also need a button kit for ISH PROBE because the user-fillable dispenser will not have the proper commercial bar code. One more thing, UltraView Red ISH DIG Detection
[Histonet] RE: Histonet Digest, Vol 99, Issue 11
Anita- We started off with taking them down every other week and moved to once a month schedule and have had no problems, but you'd want to keep a complete defrost in mind if persistent sectioning problems ever arise. Martha ~ Martha Wester Translational Sciences MedImmune Message: 2 Date: Wed, 8 Feb 2012 16:49:29 -0600 From: anita dudley azdud...@hotmail.com Subject: [Histonet] cryostats decontamination To: histonet@lists.utsouthwestern.edu Message-ID: blu163-w55b7dea6e8729d2249fc95d1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re-send Darker /or granular hematoxylin staining (no photo)
Hello my colleagues, Any input will be much appreciated - what can cause darker and/or granular hematoxylin staining on the goblet cells (mucin areas) of certain GI biopsies while on other similar GIs in the very same rack of slides, they stain water clear which is the preferred outcome. I use regressive HE staining using Sigma Aldrich Harris hemtoxylin standard 1% Acid alcohol (hydrocholric acid). I tried stronger acid for differentiation, cut thinner, longer time in acid differentiation, less hematoxylin time, to try to get the mucin staining as minimal as possible but I can't seem to eliminate the darker /or more granular staining on some GIs. Thanks in advance for any thoughts you might have! Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. 503.443.2157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re-send Darker /or granular hematoxylin staining (no photo)
I think these granules are of insoluble hematoxylin or preceptated hematoxylin granules a had these condition 2 years ago and resolved it by prepairing new one. hope these could help Mohamed Abd el Razik Ass. Lec. of veterinary histology Cairo University- Egypt From: Rolly Perez rpe...@medsurgpath.com To: HISTONET histonet@lists.utsouthwestern.edu Sent: Thursday, February 9, 2012 11:34 PM Subject: [Histonet] Re-send Darker /or granular hematoxylin staining (no photo) Hello my colleagues, Any input will be much appreciated - what can cause darker and/or granular hematoxylin staining on the goblet cells (mucin areas) of certain GI biopsies while on other similar GIs in the very same rack of slides, they stain water clear which is the preferred outcome. I use regressive HE staining using Sigma Aldrich Harris hemtoxylin standard 1% Acid alcohol (hydrocholric acid). I tried stronger acid for differentiation, cut thinner, longer time in acid differentiation, less hematoxylin time, to try to get the mucin staining as minimal as possible but I can't seem to eliminate the darker /or more granular staining on some GIs. Thanks in advance for any thoughts you might have! Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. 503.443.2157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostats decontaminationIn-Reply-To=
We use an Isopropanol based spray called DisCide Ultra Disinfecting Spray, ColePalmer catolog # EW-86306-12. Or direct from the company that makes it, www.palmerohealth.com. The spray bottle that it comes in puts out a very fine, wide spay of the disinfectant which gets it into all those hard to reach spots of a cryostat. We do this weekly along with the UV light decontamination that one of our cryotstat is equiped with. We do a more extensive/take down decontamination monthly. amysue ruppert __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
