[Histonet] RE: Opinions on Leica stainer
Workhorse...has all the requirements we need. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giracello, Darlene Sent: Thursday, February 16, 2012 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on Leica stainer We would appreciate any opinions on the Leica Autostainer XL ST5010 and the ST5020. Any likes, dislikes, has it been reliable? Consumables? Service? We are a veterinary diagnostic lab, so flexibility is very important for us. I bow to the wisdom of Histonet!!! Thanks Darlene ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Opinions on Leica stainer
We love ours!! Works great!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Friday, February 17, 2012 3:12 AM To: darlene.girace...@okstate.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Opinions on Leica stainer Workhorse...has all the requirements we need. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giracello, Darlene Sent: Thursday, February 16, 2012 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on Leica stainer We would appreciate any opinions on the Leica Autostainer XL ST5010 and the ST5020. Any likes, dislikes, has it been reliable? Consumables? Service? We are a veterinary diagnostic lab, so flexibility is very important for us. I bow to the wisdom of Histonet!!! Thanks Darlene ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting Tendon
Dear listers, I am trying to get transversal sections of cow flexor digital tendons. I need sections of the whole piece, about 1cm diameter. Before paraffin embedding I have treated the samples with: -KOH 4% -4% phenol in 70% ethanol In both cases the results are very poor; the tissue is torn to pieces. I would very grateful for any input regarding this issue. Best Regards Pablo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana xt
I think the Ventana XT is the best instrument for adding slides as you go which makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is so easy to use it is virtually impossible to mess up with it's bar coding and visual control. Very user friendly. Jan Mahoney,Omaha, NE From: christiego...@msn.com To: dphill...@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 19:46:35 + Subject: RE: [Histonet] Ventana xt CC: Pros: Lends itself well to labs that do basic IHC staining Antibodies are pre-diluted so no guess work on dilutions Good for batching runs Consistent quality of slides 24 hour technical support Can run molecular probes protocols are easy to adjust Cons: Closed system Must use Ventana antibodies or purchase special dispensers if using non Ventana products Pre-dilute antibodies are pricey Stand alone instrument so must have space for it I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. Christie Gowan UAB Hospital From: dphill...@vetmed.lsu.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 12:29:37 -0600 Subject: [Histonet] Ventana xt Looking for any pros and cons on the Ventana XT. Thanks Del ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana xt
Side note: only the Ventana Ultra allows adding slides as you go, not the XT. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, February 17, 2012 7:52 AM To: Christie Gowan; dphill...@vetmed.lsu.edu; histonet Subject: RE: [Histonet] Ventana xt I think the Ventana XT is the best instrument for adding slides as you go which makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is so easy to use it is virtually impossible to mess up with it's bar coding and visual control. Very user friendly. Jan Mahoney,Omaha, NE From: christiego...@msn.com To: dphill...@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 19:46:35 + Subject: RE: [Histonet] Ventana xt CC: Pros: Lends itself well to labs that do basic IHC staining Antibodies are pre-diluted so no guess work on dilutions Good for batching runs Consistent quality of slides 24 hour technical support Can run molecular probes protocols are easy to adjust Cons: Closed system Must use Ventana antibodies or purchase special dispensers if using non Ventana products Pre-dilute antibodies are pricey Stand alone instrument so must have space for it I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. Christie Gowan UAB Hospital From: dphill...@vetmed.lsu.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 12:29:37 -0600 Subject: [Histonet] Ventana xt Looking for any pros and cons on the Ventana XT. Thanks Del ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue processors
For your requirements, Sakura VIP is the bast, at least for me. René J. --- On Thu, 2/16/12, Bales, Candy A candy.a.ba...@uth.tmc.edu wrote: From: Bales, Candy A candy.a.ba...@uth.tmc.edu Subject: [Histonet] tissue processors To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, February 16, 2012, 10:31 AM Good morning. These questions are on behalf of a friend who is in the market for a new tissue processor. She wants to know the pros and cons of the various processors in use. She mentioned the Excelsior, VIP Leica but is open to other suggestions. The workload is approximately 200 blocks or less per night. She is looking for something the doctors can easily stop and add cassettes to and restart. She is also looking to replace her old linear stainer, no longer being produced by Thermo-Shandon. Does anyone know of a company producing a linear stainer? Or for those who have auto stainers, if you could give her the pros and cons of the models you use. Thanking you in advance Candy Bales, HT Chief Histologist The University of Texas Health Science Center Houston- School of Dentistry Diagnostic Biomedical Sciences 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting Tendon
First of all fixation has to be perfect, if with NBF, at least 72 hours. Then increase twice your standard processing times, specially infiltration. Substitute your normal paraffin was to one of 63-65ºC and chill be blocks before sectioning. René J. --- On Fri, 2/17/12, Pablo Sanchez-Quinteiro pablo.sanc...@usc.es wrote: From: Pablo Sanchez-Quinteiro pablo.sanc...@usc.es Subject: [Histonet] Cutting Tendon To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 6:13 AM Dear listers, I am trying to get transversal sections of cow flexor digital tendons. I need sections of the whole piece, about 1cm diameter. Before paraffin embedding I have treated the samples with: - KOH 4% - 4% phenol in 70% ethanol In both cases the results are very poor; the tissue is torn to pieces. I would very grateful for any input regarding this issue. Best Regards Pablo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position
Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@carisls.com http://webmail.windstreamhosting.com/hwebmail/services/go.php?url=http% 3A%2F%2Fmailto%3Amhale%40carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Union positions?
Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC and negative controls?
We are not using negative controls. We only add positive controls at the bottom of our patient slides. Sometimes there are 3 different types of control tissue (a TMA), so that you can use the same control block for several antibodies. You could add a negative control in the same block as the positive control and add these at the bottom of your slides (or at the top, if you wish). Willem Hoekert OLVG The Netherlands Van: histonet-boun...@lists.utsouthwestern.edu namens Tom McNemar Verzonden: do 16-2-2012 16:27 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org file:///C:/Documents%20and%20Settings/TMCNEMAR/Application%20Data/Microsoft/Signatures/www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anti static gun
Available through VWR, but may be cheaper elsewhere. They work much better than I expected. ZEROSTAT ANTISTATIC GUN VWR cat #100496-120 Vendor cat #60610 Each$122.64 list Donna Harclerode, HTL, QIHC, SLS (ASCP) Associate Scientist Celgene 9393 Towne Center Road San Diego, CA 92121 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Union positions?
Union position? Only if I was starving. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Bakken Sent: Friday, February 17, 2012 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Union positions? Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Union positions?
We have 17 histotech over 1000 lab techs ( classified as Medical Technologists) and NO union Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of angela smith [we3smi...@yahoo.com] Sent: Friday, February 17, 2012 11:46 AM To: histonet@lists.utsouthwestern.edu; Pam Bakken Subject: Re: [Histonet] Union positions? I am a lab of 5 histotechs and no union. I would never accept a position that is union! --- On Fri, 2/17/12, Pam Bakken pam.bak...@childrensmn.org wrote: From: Pam Bakken pam.bak...@childrensmn.org Subject: [Histonet] Union positions? To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 10:53 AM Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana XT Closed System misnomer
Eric, I wouldn't say my mind is closed on the issue and I appreciate your thoughts and opinions. I agree wholeheartedly about the advantages of the XT and to say that the Benchmark XT is a closed system is simply to state that it is more closed regarding reagents than some systems (open) where the reagents are purchsed from various vendors in whatever form you choose. Some people who use all non-Ventana antibodies may find this as a con vs. a pro when it comes to considering purchasing an XT for their lab. Thanks. Christie Gowan HT(ASCP) Date: Thu, 16 Feb 2012 15:41:35 -0500 From: gagn...@kgh.kari.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana XT Closed System misnomer Responding to Del who asked about pros/cons of Ventana BenchMark XT: Ventana is often equated with 'closed system'. This is a misnomer. The only thing I regard as closed (besides some colleagues' minds on this issue) is the detection system kit and bulk reagents that are used on the instrument. While these are proprietary, they are also of extremely high quality, resiliency, consistency, and in my opinion, dependability. Preparing one's own reagents can lead to a lack of consistency of staining, and we have confidence in Ventana reagents. As others have correctly noted...other non-Ventana antibodies can be used in Ventana dispensers. We use an equal mix of ready-to-use antibodies with our own diluted antibodies from other suppliers. How is this a closed system? Pre-treatments can also be used in Ventana dispensers. There is a range of non-ab reagents such as hematoxylins and proteases. There are a range of detection system kits as well - at least four that I can think of. Perhaps this sounds like my personal crusade, as a Ventana user; to try to demolish the 'closed system' myth. In my professional interactions with non-Ventana users, this is often heard as a derogatory, negative reaction to Ventana, which I certainly speak truth to at every opportunity. Del, we have found the BenchMark XT's very reliable, with good support and service, plus excellent quality reagents including antibodies. Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingson, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cell Block
Happy Friday Histonetters, We have a pathologist that will be ordering all the latest test such as MMR - ALK Mutation and all of the other good ones that oncologist will be relying on to treat their patients. He wants to order these test on cell block material that he gets from FNA's. As is we get scant material from FNA's for cell block preparation. Question is: What are other facilities doing to maximize their yield on cell blocks? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Humidity levels and IHC staining
We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Humidity levels and IHC staining
A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
We use the Dako Autostainer. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -Original Message- From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Friday, February 17, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
Jason, We've experienced similar problems a few summers ago when the environmental conditions inside the Autostainers (dako) reach high temperatures and low humidity. We keep thermometers inside the stainers to monitor these levels. To remedy this problem, before every IHC run, I add a few damp paper towels in the stainer sink. As the stain progresses, liquid is added to the paper towels by the stainer so I never have to worry about it drying out. Since we've started doing this, the humidity ranges anywhere from 35-55% and an ending temperature of no more than 28C. With these conditions, we've yet to experience slides drying during a staining run. Another factor to consider is volume of reagent added to each slide, we do 100 ul per drop zone (3 drop zones total). The longest a reagent left on a slide is 1 hour max for a few of our primary antibodies. Hope this helps, Ross From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jason McGough [jmcgo...@clinlab.com] Sent: Friday, February 17, 2012 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels and IHC staining We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: [Histonet] Humidity levels and IHC staining
I've had 3 Dakos and have had the drying phenomenon in all of them to some extent. We put wet paper towels in the stain sink, as others have mentioned. Plus, we found that most of the problem came from having an air gap between the hood and the unit, which wasn't closed entirely by the gasket. (The circulating room air got through the gap and dried out the slides in that front row area.) We use a long piece of styrofoam padding, cut it to length, and insert that between the bottom of the closed hood and the metal base at the beginning of each staining run, and it blocks all unwanted airflow into the slide chamber. Jan Shivers UMN -- Forwarded message -- From: Cynthia Pyse cp...@x-celllab.com Date: Fri, Feb 17, 2012 at 1:39 PM Subject: RE: [Histonet] Humidity levels and IHC staining To: Morken, Timothy timothy.mor...@ucsfmedctr.org, Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu, Jason McGough jmcgo...@clinlab.com NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Fwd: [Histonet] Humidity levels and IHC staining
We have the Lab Vision 360 from Thermo Fisher and we have really bad problems with the slides drying out. We put wet paper towels and water boats in, but after 20 or 30 minutes, the slides have very little liquid on them. I have gone back a lot to manual staining except for stains that work at 30 minutes. By the way, we are in Ca. Has anyone else that has this instrument have the same problem? Nancy From: Jan Shivers shive...@umn.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Friday, February 17, 2012 11:56 AM Subject: Fwd: [Histonet] Humidity levels and IHC staining I've had 3 Dakos and have had the drying phenomenon in all of them to some extent. We put wet paper towels in the stain sink, as others have mentioned. Plus, we found that most of the problem came from having an air gap between the hood and the unit, which wasn't closed entirely by the gasket. (The circulating room air got through the gap and dried out the slides in that front row area.) We use a long piece of styrofoam padding, cut it to length, and insert that between the bottom of the closed hood and the metal base at the beginning of each staining run, and it blocks all unwanted airflow into the slide chamber. Jan Shivers UMN -- Forwarded message -- From: Cynthia Pyse cp...@x-celllab.com Date: Fri, Feb 17, 2012 at 1:39 PM Subject: RE: [Histonet] Humidity levels and IHC staining To: Morken, Timothy timothy.mor...@ucsfmedctr.org, Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu, Jason McGough jmcgo...@clinlab.com NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___
[Histonet] Union positions?
Pam, I believe only the city morgue on Oahu have the histotech-type positions in Hawaii that are union. There are about 48 histotechs or histotech-like postions in 14 centers across 4 of 8 Hawaiian islands (the other 4 islands are too small to support full medical facilities-and one is still under water). We have federal, state, city/county and Kaiser Permanante, positions that most think of being unionized. Most of us were offered to join, but union tactics and dues were not really what we got into health care for. Hugh Luk, HTL (ASCP) UH Cancer Center- PSR lab manager Honolulu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Bakken Sent: Friday, February 17, 2012 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Union positions? Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
Hi; We had the same problem, but are in the dry state of Colorado. I have a couple of suggestions. Make sure that the seal on your lid is adequate, as overtime they can rot. Also, make sure that your racks are not warped. Warped racks can cause some of the liquid to leak out of the sides. We also put a shallow pan of water during runs just to humidify the air a bit. Good luck, Tracy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, February 17, 2012 12:39 PM To: 'Morken, Timothy'; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; 'Jason McGough' Subject: RE: [Histonet] Humidity levels and IHC staining NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana xt
Thanks for correcting me.Jan Subject: RE: [Histonet] Ventana xt Date: Fri, 17 Feb 2012 08:23:33 -0600 From: lseb...@uwhealth.org To: mamaw...@hotmail.com; christiego...@msn.com; dphill...@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu Side note: only the Ventana Ultra allows adding slides as you go, not the XT. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, February 17, 2012 7:52 AM To: Christie Gowan; dphill...@vetmed.lsu.edu; histonet Subject: RE: [Histonet] Ventana xt I think the Ventana XT is the best instrument for adding slides as you go which makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is so easy to use it is virtually impossible to mess up with it's bar coding and visual control. Very user friendly. Jan Mahoney,Omaha, NE From: christiego...@msn.com To: dphill...@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 19:46:35 + Subject: RE: [Histonet] Ventana xt CC: Pros: Lends itself well to labs that do basic IHC staining Antibodies are pre-diluted so no guess work on dilutions Good for batching runs Consistent quality of slides 24 hour technical support Can run molecular probes protocols are easy to adjust Cons: Closed system Must use Ventana antibodies or purchase special dispensers if using non Ventana products Pre-dilute antibodies are pricey Stand alone instrument so must have space for it I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. Christie Gowan UAB Hospital From: dphill...@vetmed.lsu.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 12:29:37 -0600 Subject: [Histonet] Ventana xt Looking for any pros and cons on the Ventana XT. Thanks Del ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
