[Histonet] 101 steps to better histology
Hi all, Just thought I'd share this with y'all. I have just received a booklet from Leica Microssytems 101 steps to better histology, which i think is an excellent overview and ready reference for practical histology. It has great photos of common problems such as sections contaminated with squames, incomplete blueing, bubbles in sections etc. Disclaimer: This is my personal opinion. I have no interests in Leica other than purchasing their equipment now and then -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] human samples in research lab
Hi all, I would like to get some advice from the experts. We are a research lab currently working primarily with mouse and zebrafish tissues. One of our researchers has access to tissues from local hospitals which have been processed and embedded. Having been in research and away from the clinical field for over 20 years, I am unaware of the PPE and required safety training required for working with primary human tissues. We currently observe reasonable safety measures such as lab coats and glasses when handling chemicals, and of course no eating, drinking, or open-toed shoes in the lab. But should we be wearing lab coats, glasses and gloves while sectioning human samples? And what about the legal aspects such as material transfer agreements and HIPAA rules? I have been avoiding this issue for years by encouraging researchers to have the slides cut at the hospitals supplying the tissues, but I may not be able to do this much longer. Any advice? Thanks, Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pinning Specimen
Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Citrate Buffer pH2.0
Hi Eric Thanks for your input regarding the Citrate buffer pH2.0. Yep this pH came as a surprise to me too. The data sheet from the company specifies 0.01M citrate at pH2.0 and a publication which used the same antibody also indicates a modified citrate buffer but doesn't give the exact p. I will be checking the information with the company as suggested but just wanted to see if anyone else out in Histoland had come across this previously. So far the unexpected indication is No. I'll let you know how I get on if I do have to use pH 2.0 and if it is successful. Best regards Neil MacIntyre CSci FIBMS Laboratory Manager Veterinary Pathology Unit The Royal(Dick)School of Veterinary Studies The University Of Edinburgh Easter Bush Campus Midlothian EH25 9RG 0131 650 6403/8802 The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pinning Specimen
We used to make our own. We would get a large shallow cardboard box, fill it with paraffin. Let it cool. Then peel the cardboard away You can submerse that in formalin. And you can custom cut them to whatever size you need. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF [karen.heckf...@dignityhealth.org] Sent: Thursday, April 19, 2012 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pinning Specimen Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pinning Specimen
Hi Karen - Are yoru eferring to the boards that have the detachable mats? I know they still make them and the last one I heard of was through MOPEC. I'm probably aging myself here, but we also used to make paraffin blocks for large specimens and pin them flat for overnight fixation - it wasn't pretty but it worked. Hope this helps some. Vikki On Thu, Apr 19, 2012 at 8:03 AM, Heckford, Karen - SMMC-SF karen.heckf...@dignityhealth.org wrote: Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pinning Specimen
At previous institutions I purchased rolls or sheets of cork and that allowed us to pin and float samples like neck dissections, segments of colon etc I know Fisher sells the sheets and the roll of cork we purchased from an internet retailer. Just do a Google or Amazon search. Making the paraffin blocks is easy as well. You can take any plastic container and pour off fresh paraffin or use paraffin from a tissue processor when you are disposing of it to make molds. Hope this helps. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker [bakevicto...@gmail.com] Sent: Thursday, April 19, 2012 9:16 AM To: Heckford, Karen - SMMC-SF Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pinning Specimen Hi Karen - Are yoru eferring to the boards that have the detachable mats? I know they still make them and the last one I heard of was through MOPEC. I'm probably aging myself here, but we also used to make paraffin blocks for large specimens and pin them flat for overnight fixation - it wasn't pretty but it worked. Hope this helps some. Vikki On Thu, Apr 19, 2012 at 8:03 AM, Heckford, Karen - SMMC-SF karen.heckf...@dignityhealth.org wrote: Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Batch Controls
All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no gaurantee that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Batch Controls
I still use batch controls, but I am one of the few left that is not automated. I do everything by hand. I think you are right though, placing a control tissue on each slide is the only way to be sure that everything was dispensed correctly...especially if you are using a Ventana... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Thursday, April 19, 2012 9:20 AM To: histonet Subject: RE: [Histonet] Batch Controls All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no gaurantee that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Batch Controls
After spending 7 1/2 years as a technical specialist with Ventana, I would also like to say that putting the control on the bottom of the slide and the patient at the top is just added insurance that the patient will receive the bulk of the reagents vs. the control which will help ensure against false negatives should there be a staining issue. Just my 2 cents worth. thanks Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.436.1369 dsi...@statlab.com | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Thursday, April 19, 2012 9:20 AM To: histonet Subject: RE: [Histonet] Batch Controls All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no gaurantee that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shandon Hypercenter XP processor
Do any of you know of companies or individuals that can repair an old Shandon Hypercenter XP processor? They don't even make parts for it anymore. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Problem
Sounds like the specimens are not fixed well enough! Josie Britton HT(ASCP) Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Wednesday, April 18, 2012 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Problem OK netters, I've got a problem that's just cropped up over the last week or so. It's happening to our larger specimens, and only the ones containing tumor. When we cut a slide, the tissue looks processed well, but as the slide dries, the tissue area turns really white. Then when we try and stain it (especially Immunos) just that portion of the section falls off. Any clues to defeat this gremlin would be greatly appreciated. Dan Daniel R Peterson HT(ASCP) Histopathology Technical Specialist Meriter Laboratories (608) 417-6557 fax (608) 417-6343 1dpeter...@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leased tech to GI practice
I am wondering if any anatomic pathology labs out there have leased a FTE, histology tech, to a GI practice to run a histology lab in the Endoscopy clinic? How did you logistically accomplish this? You can contact me offline for more details. Thank you in advance for your responses. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] grossing tech
I have a grossing position open, I have a non certified. Non degreed awesome tech with 27 years. Anyway I can have her Gran-fathered in by CAP regulations? I'm pretty sure I know the answer I am hoping to get around this, Cheri Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Unregistered HT testing
I disagree with your assessment of complex staining. IHC staining is like cookie-cutter staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, April 17, 2012 1:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Unregistered HT testing
Tresa I do see your point. I guess to me setting up IHC and validation takes some insight and knowledge, but once it is running on a platform, it is pretty much the same as far as your hands on and gets pretty routine. Those that get to still do manual IHC get a little more challenge. Since I just do automated and I am not allowed to participate or alter protocols even when I think I should, it does get pretty repetitive, and of course you are right- its basically the same groups of reagents and reaction you carry out, albeit with different more specific targets. I do personally think that doing specials does require a lot more chemistry/ reaction knowledge, and tissue knowledge, since each stain has a unique chemistry, reaction, results etc. I especially believe that for manual special stains. Remember when you had to differeniate stains under the microscope? Nowadays I see many techs that never even look under the 'scope! Anyhow, that is why I liked doing them that way best, and I really kind of miss it. I wish that people still had to know why/what/how of doing those stains even if they use an instrument to carry them out, but that is opinion not necessarily shared by everyone. I think using automation for either specials or IHC can make you lazy in a way, though it helps standardization and TAT. But unfortunately, the powers that be don't seem to see it that way. As many have pointed out, CLIA does not recognize anything in histology as high complexity except for IHC/ISH. Of course it was put out in 1988 and probably no histology/legislators were involved in the categorization of testing. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tgo...@mt.gov To: nko...@chw.org; histonet@lists.utsouthwestern.edu Date: Thu, 19 Apr 2012 16:33:46 + CC: Subject: [Histonet] RE: Unregistered HT testing I disagree with your assessment of complex staining. IHC staining is like cookie-cutter staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, April 17, 2012 1:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Unregistered HT testing
As usual we all have our own opinion. High complexity acceding to CLIA is a defined measurement. In other words things like is the task heat, ph , time dependent, accurate measuring ? Those examples make a task high complexity. The above us exactly why grossing is considered high complexity by CLIA. Don't forget that CAP standards are equivalent or higher than CLIA And the important difference to remember is not that someone knows how to push a button. But they know what to do when the button won't work I hope all of us as professionals always seek to raise the standards and education of our task and not to always seek less of what is expected of us. Have a good one! Kim D Sent from my iPhone On Apr 19, 2012, at 1:20 PM, joelle weaver joellewea...@hotmail.com wrote: Tresa I do see your point. I guess to me setting up IHC and validation takes some insight and knowledge, but once it is running on a platform, it is pretty much the same as far as your hands on and gets pretty routine. Those that get to still do manual IHC get a little more challenge. Since I just do automated and I am not allowed to participate or alter protocols even when I think I should, it does get pretty repetitive, and of course you are right- its basically the same groups of reagents and reaction you carry out, albeit with different more specific targets. I do personally think that doing specials does require a lot more chemistry/ reaction knowledge, and tissue knowledge, since each stain has a unique chemistry, reaction, results etc. I especially believe that for manual special stains. Remember when you had to differeniate stains under the microscope? Nowadays I see many techs that never even look under the 'scope! Anyhow, that is why I liked doing them that way best, and I really kind of miss it. I wish that people still had to know why/what/how of doing those stains even if they use an instrument to carry them out, but that is opinion not necessarily shared by everyone. I think using automation for either specials or IHC can make you lazy in a way, though it helps standardization and TAT. But unfortunately, the powers that be don't seem to see it that way. As many have pointed out, CLIA does not recognize anything in histology as high complexity except for IHC/ISH. Of course it was put out in 1988 and probably no histology/legislators were involved in the categorization of testing. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tgo...@mt.gov To: nko...@chw.org; histonet@lists.utsouthwestern.edu Date: Thu, 19 Apr 2012 16:33:46 + CC: Subject: [Histonet] RE: Unregistered HT testing I disagree with your assessment of complex staining. IHC staining is like cookie-cutter staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, April 17, 2012 1:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Unregistered HT testing
Yes, I wish for that too. Joelle Weaver MAOM, HTL (ASCP) QIHC CC: tgo...@mt.gov; nko...@chw.org; histonet@lists.utsouthwestern.edu From: one_angel_sec...@yahoo.com Subject: Re: [Histonet] RE: Unregistered HT testing Date: Thu, 19 Apr 2012 13:31:07 -0400 To: joellewea...@hotmail.com As usual we all have our own opinion. High complexity acceding to CLIA is a defined measurement. In other words things like is the task heat, ph , time dependent, accurate measuring ? Those examples make a task high complexity. The above us exactly why grossing is considered high complexity by CLIA. Don't forget that CAP standards are equivalent or higher than CLIA And the important difference to remember is not that someone knows how to push a button. But they know what to do when the button won't work I hope all of us as professionals always seek to raise the standards and education of our task and not to always seek less of what is expected of us. Have a good one! Kim D Sent from my iPhone On Apr 19, 2012, at 1:20 PM, joelle weaver joellewea...@hotmail.com wrote: Tresa I do see your point. I guess to me setting up IHC and validation takes some insight and knowledge, but once it is running on a platform, it is pretty much the same as far as your hands on and gets pretty routine. Those that get to still do manual IHC get a little more challenge. Since I just do automated and I am not allowed to participate or alter protocols even when I think I should, it does get pretty repetitive, and of course you are right- its basically the same groups of reagents and reaction you carry out, albeit with different more specific targets. I do personally think that doing specials does require a lot more chemistry/ reaction knowledge, and tissue knowledge, since each stain has a unique chemistry, reaction, results etc. I especially believe that for manual special stains. Remember when you had to differeniate stains under the microscope? Nowadays I see many techs that never even look under the 'scope! Anyhow, that is why I liked doing them that way best, and I really kind of miss it. I wish that people still had to know why/what/how of doing those stains even if they use an instrument to carry them out, but that is opinion not necessarily shared by everyone. I think using automation for either specials or IHC can make you lazy in a way, though it helps standardization and TAT. But unfortunately, the powers that be don't seem to see it that way. As many have pointed out, CLIA does not recognize anything in histology as high complexity except for IHC/ISH. Of course it was put out in 1988 and probably no histology/legislators were involved in the categorization of testing. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tgo...@mt.gov To: nko...@chw.org; histonet@lists.utsouthwestern.edu Date: Thu, 19 Apr 2012 16:33:46 + CC: Subject: [Histonet] RE: Unregistered HT testing I disagree with your assessment of complex staining. IHC staining is like cookie-cutter staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, April 17, 2012 1:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Manager position with our clinical reference lab client in southern California
Our client is a leading clinical laboratory currently seeking a Histology/IHC Manager, Monday thru Friday, to oversee operation administration of several departments. Based in southern California, this individual will work closely with pathologists other medical professionals to effectively meet operation goals, work with tight timelines and ensure that histology staff adheres to these needs. while conducting high quality work. Specimens: slides, blocks, previously cutsome Grossing.Bone Marrow and Aspirate. Responsibilities will include: . Management of all day-to-day operations technical activities of IHC/Histology/Imaging Department, including staffing, planning, coordination and evaluation . Troubleshooting quality and production issues including stains, processes, systems, and equipment. . Developing and implementing improvements in systems and processes for enhanced efficiency and quality, against corporate and departmental and goals. Candidate must have BA/BS in Biology, Chemistry or related science, with 4+ years of experience in a clinical lab including at least 3 years of supervisory experience. HT (ASCP); and QIHC qualification helpful. Must meet CLIA requirements. In addition, superior organizational and operational skills and the ability to manage multiple priorities and timelines effectively are needed. Qualities in this individual will be: self-motivated, detail oriented, excellent communication skills, and thriving with high degree of responsibility. Offered for this position is compensation commensurate with experience to attract top talent, with bonus package, relocation assistance for California licensed individual. .Please contact David King at mailto:biolabcare...@aol.com biolabcare...@aol.com for more information about this opportunity. David King Career Studio national search mailto:biolabcare...@aol.com biolabcare...@aol.com 561-738-6363 Visit us on linkedin: http://www.linkedin.com/in/biotechnologyhires http://www.linkedin.com/in/biotechnologyhires ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura VIP 3000 Tissue Processor
Marston Technical from Cincinnati, Ohio has supplies. Their phone is 513-563-8100. On Wed, Apr 18, 2012 at 3:35 PM, Lyn Stadler lstad...@cbiolabs.com wrote: Anyone out there using a VIP 3000? I need some replacement gaskets that Sakuara no longer manufactures. Anyone know of an alternate vendor or supplier that carries them? Or, better yet, anyone have some that they are interested in giving away ;). Thanks in advance! This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Rhonda Ford, Histology Lab Henry County Hospital (765) 521-1148 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS on Toenail
Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: GMS on Toenail
After cutting, try putting the slide into a coplin jar of formalin. Introduce to heat for about 30 minutes or so, remove and let air dry before staining. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [allison_sc...@hchd.tmc.edu] Sent: Thursday, April 19, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Pinning Specimen
Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco asks: Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. I've solved this problem several ways. Probably the best, which has already been mentioned, is to have blocks cast from waste paraffin, in several sizes, and pin the specimen to them with those T-shaped steel map pins and put it face down in the fixative. You can also use cardboard or styrofoam. You may need to put a weight on top of the pinning board. Most specimens need fixing overnight. You can ink before or after fixation. Most of the pathology services I've worked in do not have such pinning arrangements and do not welcome them. Does anyone know what the Hospital Administrator and Lab Manager's Handy-Dandy Manual for Tying the Pathologist in Knots has to say about them? (The top-secret book they all have in their desk drawers.) Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] proliferation and apoptosis
Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hepes instead of cacodylate
Hi I'm trying to switch from cacodylate buffer to Hepes in my EM-fixative. PBS buffer is not an option. My fixative contains sucrose, CaCl2, 2,5 % formaldehyde and 2,5% GA in addition to cacodylate (later Hepes). Formaldehyde is homemade stock 25 % (no methanol) After storage for some time in Hepes fixative I see a turbidity/precipitate. Stored fixative with precipitate and newly mixed fixative gave the same pH. Another group who made this Hepes fixative after my recipe told me they got precipitate once they mixed in the GA. What have I missed here when it comes to chemicals and reactions? Any good protocols out there? Tora Bardal NTNU Sealab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] proliferation and apoptosis
Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. Jackie -Original Message- From: Margaryan, Naira nmargar...@childrensmemorial.org To: histonet-request histonet-requ...@lists.utsouthwestern.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thu, Apr 19, 2012 3:09 pm Subject: [Histonet] proliferation and apoptosis Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: GMS on Toenail
Are using any adhesive on the slide or a charged slide? We used to dip slides in a water/elmer glue solution and allow to dry. Then place a section on it and it seemed to work... Sent from my HTC on the Now Network from Sprint! - Reply message - From: Kienitz, Kari kkien...@orclinic.com Date: Thu, Apr 19, 2012 12:33 pm Subject: [Histonet] RE: GMS on Toenail To: Scott, Allison D allison_sc...@hchd.tmc.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu After cutting, try putting the slide into a coplin jar of formalin. Introduce to heat for about 30 minutes or so, remove and let air dry before staining. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [allison_sc...@hchd.tmc.edu] Sent: Thursday, April 19, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] proliferation and apoptosis
I agree with Jackie, we use Ki-67 all of the time and I have never seen it stain apoptotic cells, could you possibly be dealing with some background staining due to the detection system used? I do not know what type of samples you are staining? We have 4 different Ki-67 antibodies we use depending upon the type of tissue we are staining. For human xenograft samples in a mouse background we use the rabbit polyclonal from Santa Cruz. We also use cleaved caspase 3 as a marker of apoptosis routinely. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, April 19, 2012 3:23 PM To: nmargar...@childrensmemorial.org; histonet-requ...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] proliferation and apoptosis Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. Jackie -Original Message- From: Margaryan, Naira nmargar...@childrensmemorial.org To: histonet-request histonet-requ...@lists.utsouthwestern.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thu, Apr 19, 2012 3:09 pm Subject: [Histonet] proliferation and apoptosis Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LIS
Anyone know if there is or has used a good Anatomical Path LIS that is compatible or user friendly with Greenway. I really don't know a whole lot about this stuff , but could use some ideas. Thanks Histojoe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] proliferation and apoptosis
I've used pHH3 to mark proliferative cells and it works pretty well. Active-caspase-3 and cleaved-parp have worked well for staining apoptotic cells as well. -Mehlika From: l...@premierlab.com To: b427...@aol.com; nmargar...@childrensmemorial.org; histonet-requ...@lists.utsouthwestern.edu Date: Thu, 19 Apr 2012 15:37:26 -0600 Subject: RE: [Histonet] proliferation and apoptosis CC: histonet@lists.utsouthwestern.edu I agree with Jackie, we use Ki-67 all of the time and I have never seen it stain apoptotic cells, could you possibly be dealing with some background staining due to the detection system used? I do not know what type of samples you are staining? We have 4 different Ki-67 antibodies we use depending upon the type of tissue we are staining. For human xenograft samples in a mouse background we use the rabbit polyclonal from Santa Cruz. We also use cleaved caspase 3 as a marker of apoptosis routinely. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, April 19, 2012 3:23 PM To: nmargar...@childrensmemorial.org; histonet-requ...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] proliferation and apoptosis Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. Jackie -Original Message- From: Margaryan, Naira nmargar...@childrensmemorial.org To: histonet-request histonet-requ...@lists.utsouthwestern.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thu, Apr 19, 2012 3:09 pm Subject: [Histonet] proliferation and apoptosis Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
