[Histonet] Translucent patch
Hello, We have recently noticed strange translucent patches that can be seen within our 3 micron tissue sections when they are floated out. From the surface of the water bath they look like holes within the tissue but when the slides are viewed, the tissue is still present. This region of tissue however, creases and folds when mounted. The rest of the section looks and behaves perfectly - it is only this translucent region that is causing the problem. I have seen this region as a small blob but also as a thin streak that runs through the entire section. This region can only be seen for the first 7-10 sections taken. After this the translucent region gets smaller and disappears. This is making getting perfectly flat, artefact free sections of tissue (mouse / rat gut / liver) difficult. Does anyone have any idea what this could be? My thoughts are a possibly processing issue - currently process without vacuum wax infiltration or an embedding issue. Our metal moulds were cleaned with a methanol based para-release spray about 2 months ago by mistake - we have had subsequent tissue spreading issues as a result. Could traces of this affect the cutting surface of the tissue causing this patch? Any ideas would be great, Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Manual for Sorvall MT-2B Rotary Motor UltraMicrotome?
Does anyone have a manual for the Sorvall MT-2B rotary motor ultramicrotome? I have been asked to teach a student how to use it for their research, yet I have never used this ultramicrotome before. Thank you, Sara Landschoot HTL(ASCP) Medical College of Wisconsin Orthopaedic Surgery - Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help
Nancy, We've had similar issues with fatty tissue falling off of the slides while performing IHC. We use Superfrost + slides which we have found to really hold the tissue well. Also, I have learned through reading round on the Histonet that air drying doesn't completely remove the water from the middle area of a tissue section. For this reason, we no longer air dry at all unless it's a slide that was cut the day before and just happened to be air dried. Our protocol changed to cutting the slides and draining them well, then putting them in a 60 C oven for 15 minutes. Then the slides are run down to water on an automated stainer with another 15 minute time in the oven on the stainer. A specific instance when the tissue falls off, was during antigen retrieval in Trilogy in a pressure cooker. If the pressure was manually released, this would cause the Trilogy to boil and it would separate the tissue from the slide. Ourprotocol changed to 12 minutes in the pressure cooker with Trilogy, then around 8 minutes to wait for the pressure to release on it's own. We would then rinse softly in distilled water to remove the Trilogy. This also seemed to help with the issue. The combination of this has worked fairly well for us with some exceptionally stubborn tissue still attempting to fall off of the slides. I would love to hear of other's experiences and how they resolved this. I do wonder about the length of time in your oven. I had spoken with one of our Biocare reps about this when we encountered the problem and he felt that longer than 30 minutes in the oven would damage the specimen's IHC integrity. Brendal Finlay HT (ASCP) Original message- From: Cloughley-Gray, Nancy cloughl...@rvh.on.ca Date: Fri, 18 May 2012 14:02:35 -0500 To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Subject: [Histonet] Help I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histotech position
I know of a histotech position available in South Carolina. Are there any histonetters looking for work in this area? Melanie NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: CAP vs. CLIA
I usually do not comment on the histonet, however this topic is near and dear to my heart. We in Histology are support scientific staff to the Pathologist as much as the Laboratory director is to the Pathologist. Scientific duties, which a pathologist can perform (histological and cytological preparation has been delegated down) to our position. Interpretation of Controls and associated material is often left to us and the Pathologist assistant We determine what the Pathologist can interpret Our expertise directly determines patient outcome, if we are not professional in our decisions then the Pathologist is ineffective in his or hers. The Professional business of running the laboratory is performed by the medical laboratory director, and associate director. These are professional positions within the laboratory where a Pathologist has delegated the managerial responsibility to a subordinate. According to CLIA, this position does not exist, only the Pathologist can truly be the Medical laboratory director. Yet in hospitals across the country many Clinical laboratory Scientist's who don't know anything or have limited knowledge of AP are in charge of our AP labs.Their positions are listed as Laboratory Director. This subordinate is the most important person in the lab, yet they too do not turn out results. They are frequently the highest paid in the lab. Their pay is based on the designation of their scientific and managerial background. I believe we can do the same. The business of the pathology lab would be very difficult for the pathologist to handle without this symbiotic relationship of the scientific businessman to the Pathologist. Likewise a Pathologist can not perform their job without the symbiotic relationship to the histotechnologist.Thereby technically, making our jobs indispensable to the Pathologist much like the laboratory director. Our problem is history, where many pathologists trained their out of work brother in law to do the work. Many rural areas still run their labs this way. As long as there is a path to become a histotech by hiring whomsoever to do the job. We will always be left behind. So I believe if CLIA and CAP can recognize the laboratory Manager as an important professional position delegated by the Pathologist then we should be afforded the same courtesy. On Sun, May 20, 2012 at 10:37 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: I seemed to have missed something or it might have been all the fresh sea air I got in Tampa at the FSH, but I do not understand the outrage expressed towards CLIA and CAP because we are not listed as testing personnel. I applaud everyone's passion for Histotechnology and the outrage that we are not allowed to fully participate in the test system model, but I think we should be directing more of our outrage to the individuals working in Histotechnology that are not and will not take responsibility to increase the professionalism of our profession and our own acceptance of the current state of Histotechnology. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results. As good as we are and as complex parts of the Histotechnology process may be, Histotechnicians, Histotechnologists and Pathology Assistants do not meet the standard stated and do not participate in the post-analytic phase, produce and release patient results. We simply are not able to be credentialed as is the Medical Technologists and Cytotechnologist. I am not saying any one laboratory professional group is better than the other, just that to be considered testing personnel, we must be properly credentialed. Collectively, we as a discipline, science and group should be working to upgrade our education requirements and training so that we can become fully invested partners with the Pathologist. We, not CAP or CLIA, must greatly increase our professionalism before we can truly be considered competent to work in the post-analytical phase. I cannot today accept that every working Histotechnician, Histotechnologist and Pathologist Assistant is able to produce the result and release. I am quite sure that every Medical Technologist and Cytotechnologist is capable and competent to produce and release a patient result. As things stand today, Histotechnology and all of us the working in this discipline are a support function to the one person in our discipline, the Pathologist, that is educated, trained, credentialed and competent to produce and release a patient result. I also believe there are many opportunities within our process available now, such as histochemical staining for organisms, that could allow us to participate in the post-analytic step. There will be many more as personalized medicine continues to transform Histotechnology. That said, how can we honestly promote our participation in the post-analytic phase, when there are far too many
RE: [Histonet] RE: CAP vs. CLIA
Bill I have to agree with you on this, but then again we have always been looked as a step children within the lab. What I see is word play here, Cyto tech and Med Tech (CLS) are to be credited with release of a result. But because there Tech ID number is on the result they are accountable for this. As we move forward in the computer age within Anatomic Pathology we are going to be seeing the same shift, but we need our professional societies, to start to transform our profession. I am talking about algorithm analysis, special stains, IHC, bio banking, etc. There are many decisions that make us more than just a point and push tech, for lack there of a better term. I do agree education is a barrier, but once again how did the CLS (Med Tech), Cyto tech evelove? I do recall when they were taught on the job or through military training, so to say they are better because of a degree is far from the truth. Many MANY tech these days are assets to our profession and as we move forward in the future they we need to look for ways to have properly credentialed and EXPERIANCED staff. I my self am witness to the lack of basic lab knowledge a new grads have, but we are also responsible because our clinical rotation programs are scares and we do not have time to train. The future is full of opportunity for all histology tech, educated and experience, we just need to move forward and have the healthy discussion and make the changes needed in order to establish our profession. From: WILLIAM DESALVO [mailto:wdesalvo@hotmail.com] Sent: Sunday, May 20, 2012 8:38 PM To: Jesus Ellin; Timothy Morken; histonet Subject: RE: [Histonet] RE: CAP vs. CLIA I seemed to have missed something or it might have been all the fresh sea air I got in Tampa at the FSH, but I do not understand the outrage expressed towards CLIA and CAP because we are not listed as testing personnel. I applaud everyone's passion for Histotechnology and the outrage that we are not allowed to fully participate in the test system model, but I think we should be directing more of our outrage to the individuals working in Histotechnology that are not and will not take responsibility to increase the professionalism of our profession and our own acceptance of the current state of Histotechnology. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results. As good as we are and as complex parts of the Histotechnology process may be, Histotechnicians, Histotechnologists and Pathology Assistants do not meet the standard stated and do not participate in the post-analytic phase, produce and release patient results. We simply are not able to be credentialed as is the Medical Technologists and Cytotechnologist. I am not saying any one laboratory professional group is better than the other, just that to be considered testing personnel, we must be properly credentialed. Collectively, we as a discipline, science and group should be working to upgrade our education requirements and training so that we can become fully invested partners with the Pathologist. We, not CAP or CLIA, must greatly increase our professionalism before we can truly be considered competent to work in the post-analytical phase. I cannot today accept that every working Histotechnician, Histotechnologist and Pathologist Assistant is able to produce the result and release. I am quite sure that every Medical Technologist and Cytotechnologist is capable and competent to produce and release a patient result. As things stand today, Histotechnology and all of us the working in this discipline are a support function to the one person in our discipline, the Pathologist, that is educated, trained, credentialed and competent to produce and release a patient result. I also believe there are many opportunities within our process available now, such as histochemical staining for organisms, that could allow us to participate in the post-analytic step. There will be many more as personalized medicine continues to transform Histotechnology. That said, how can we honestly promote our participation in the post-analytic phase, when there are far too many individuals (good, decent and hard working) that work every day, in every type and complexity of lab, that do not have a formal secondary education, have participated in defined clinical trials or have completed a certification exam (required and necessary credentials). Just think how many practitioners of Histotechnology are out there working today that are not properly credentialed. Now think if you know of any Medical Technologist or Cytotechnologist are working that do not have the required credentials. We have many obstacles to increasing the professionalism of Histotechnology; wide and varied backgrounds, lack of standards, lack of automation, lack of certification, but I do not think
Re: [Histonet] RE: CAP vs. CLIA
William, I certainly agree that folks in both histology and pathology disciplines need to work harder at defining who they are as professionals. We, in both departments, are responsible for creating confusion. It is no wonder progress is slow in this area. If we all want to be treated more as professionals, and become better recognized, then we should start correcting the problems we allow to exist. The best place to start is with titles. - A person should not be called a histotech unless they are certified. Anyone practicing histology that is not certified should be called something else - perhaps lab assistant, or histology assistant. Regardless of experience, the tem HT should be reserved for those that are certified. If not, what is the value of certification? - Nobody should use the phrase PA or Pathologists' Assistant if they are not certified. I cannot tell you how many times I hear about the PA at this place, or that place and when I check the registry those folks are not PA's at all. This gets under the skin of each and every one of us that went through years of training, and received graduate degrees to claim that title, and is unfair to all the others that have thier degrees and completed all the requirements set forth by the ASCP and the AAPA. It seems that many like to self-promote themselves to titles they never earned. We cannot take a title simply because it is the closest description to what we do all day. I have a graduate degree in pathology, but I would never think of calling myself a pathologist. And I would be more accurate with that title than most that call themselves PA's. A graduate degree in microbiology? A Microbiologist. Graduate degree in psychology? A Psychologist. Graduate degree in biology? A Biologist. Graduate degree in Pathology? A PA. Why? Because the title Pathologist is reserved for a very specific person, with a very specific training and certification. So too should be the title HT and PA. A widespread problem in medicine is that of folks pretending to be what they are not. Some clarification is in order in all areas of medicine. Why don't we correct the problems in our own little corner and set an example? Everybody can be what they want to be, but today - you are what you are (generalization, not YOU) On Sun, May 20, 2012 at 8:37 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: I seemed to have missed something or it might have been all the fresh sea air I got in Tampa at the FSH, but I do not understand the outrage expressed towards CLIA and CAP because we are not listed as testing personnel. I applaud everyone's passion for Histotechnology and the outrage that we are not allowed to fully participate in the test system model, but I think we should be directing more of our outrage to the individuals working in Histotechnology that are not and will not take responsibility to increase the professionalism of our profession and our own acceptance of the current state of Histotechnology. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results. As good as we are and as complex parts of the Histotechnology process may be, Histotechnicians, Histotechnologists and Pathology Assistants do not meet the standard stated and do not participate in the post-analytic phase, produce and release patient results. We simply are not able to be credentialed as is the Medical Technologists and Cytotechnologist. I am not saying any one laboratory professional group is better than the other, just that to be considered testing personnel, we must be properly credentialed. Collectively, we as a discipline, science and group should be working to upgrade our education requirements and training so that we can become fully invested partners with the Pathologist. We, not CAP or CLIA, must greatly increase our professionalism before we can truly be considered competent to work in the post-analytical phase. I cannot today accept that every working Histotechnician, Histotechnologist and Pathologist Assistant is able to produce the result and release. I am quite sure that every Medical Technologist and Cytotechnologist is capable and competent to produce and release a patient result. As things stand today, Histotechnology and all of us the working in this discipline are a support function to the one person in our discipline, the Pathologist, that is educated, trained, credentialed and competent to produce and release a patient result. I also believe there are many opportunities within our process available now, such as histochemical staining for organisms, that could allow us to participate in the post-analytic step. There will be many more as personalized medicine continues to transform Histotechnology. That said, how can we honestly promote our participation in the post-analytic phase, when there are far too many
RE: [Histonet] Help
I realize that such + slides come with the instruction to completely dry slides at room temperature before placing in the drying oven. I have used these slides for many years, and have found this procedure to be not only unnecessary, but sometimes problematic. I believe sections are more likely to detach if dried at room temperature prior to oven drying. If the section is not lying perfectly flat against the glass - and some types of tissue never do initially - room temperature drying doesn't allow wrinkled areas or other problem areas to effectively spread flat. Points that are in contact with the glass bond electrostatically, but points that are separated from the glass, even by a few microns, do not. Then, when placed into the oven, such raised areas cannot spread flat because closely adjacent areas are already bonded to the slide, and cannot move. After picking up sections from the waterbath, I allow them to stand vertically and drain for no more than 5 minutes, then place them into the drying oven at 70 degrees C. for an hour. I very seldom have any detachment problems with this protocol. Paul M. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica CV5030 coverslipper issues
Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV5030 coverslipper issues
We have a refurbished CV5030 and Autostainer XL and have had no issues with them, or if so, minor ones that did not require contacting anyone. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV5030 coverslipper issues
Our CV5030 is 7 years old, and although it experiences problems occasionally, it is nothing that we felt it was necessary to have replaced. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Monday, May 21, 2012 1:57 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We have a refurbished CV5030 and Autostainer XL and have had no issues with them, or if so, minor ones that did not require contacting anyone. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV5030 coverslipper issues
We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 102, Issue 24
We used to have this problem too, till we switched to Tru-Bond slides from Tru-Scientific. Our contact is s...@tru-scientific.com . We also try to dry our slides for 1 hour in the 60 degree oven whenever possible and especially for really fatty tissues, like breast. We haven't had any problems with antigen integrity. I think greater than 60 could cook your tissue and affect things, but if you make sure it doesn't go any higher you should be OK. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 4 Date: Mon, 21 May 2012 09:25:21 -0500 From: Brendal Finlay brendal.fin...@medicalcenterclinic.com Subject: Re: [Histonet] Help To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: ea0c3755f29a151442a3fddc720e7...@medicalcenterclinic.com Content-Type: text/plain; charset=utf-8 Nancy, We've had similar issues with fatty tissue falling off of the slides while performing IHC.?? We use Superfrost + slides which we have found to really hold the tissue well.?? Also, I have learned through reading round on the??Histonet??that air drying doesn't completely remove the water from the middle area of a tissue section.?? For this reason, we no longer air dry at all unless it's a slide that was cut the day before and just happened to be air dried.?? Our protocol changed to cutting the slides and draining them well, then putting them in a 60 C oven for 15 minutes.Then??the slides are run??down to water on an automated stainer with another 15 minute time in the oven on the stainer.?? A specific instance??when the tissue falls off,??was during antigen retrieval in Trilogy in a pressure cooker.?? If the pressure was manually released, this would cause the Trilogy to boil??and??it would separate the tissue from the slide.?? Ourprotocol changed to 12 minutes in the pressure cooker with Trilogy, then around 8 minutes??to wait for the pressure to release on it's own.?? We would then rinse softly in distilled water to remove the??Trilogy.?? This also seemed to help with the issue. ?? The combination of this??has worked??fairly well for us with some exceptionally stubborn tissue still??attempting to fall off of the slides.I would love to??hear of other's experiences and??how they resolved this. I do wonder about the length of time in your oven.?? I had spoken with one of our Biocare reps about this when we encountered the problem and he felt that longer than 30 minutes in the oven would damage the specimen's IHC integrity.?? Brendal Finlay HT (ASCP) Original message- From: Cloughley-Gray, Nancy cloughl...@rvh.on.ca Date: Fri, 18 May 2012 14:02:35 -0500 To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Subject: [Histonet] Help I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV5030 coverslipper issues
We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you.
Re: [Histonet] Leica CV5030 coverslipper issues
We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken ears on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, Horn, Hazel V hor...@archildrens.org wrote: We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opportunity University of Florida
Hello Histonetters, The Anatomic Pathology Service within the University of Florida Veterinary Diagnostic Laboratories (College of Veterinary Medicine) is recruiting for Laboratory Technician for the Histology Laboratory. The work involves diagnostic veterinary histology and research histology. Full time, 40 hours per week, days, no weekends. The HT (ASCP) is not a requirement for employment. We also do not require the Florida State Laboratory License. A high school diploma and two years of appropriate experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience. Veterinary experience a plus. The University of Florida offers a generous benefits package, including one of the best pension plans in the State. Gainesville is a beautiful college town with large oaks and nearby spring fed rivers for canoeing or kayaking. Housing is very affordable. The job listing can be viewed at: https://jobs.ufl.edu/ Job title: Laboratory Technician job requisition number is 0900939 Sarahhttp://www.ufl.edu/ A. Jones, HT (ASCP) Histology Lab Manager sarahjo...@ufl.edumailto:sarahjo...@ufl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL exam
Hi, I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. Thanks! -Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P 40 Antibody
Hi, I am looking for informations on P 40 Antibody. I want informations like the vendor, procedure e.t.c. Thanks, Adesupo Adesuyi, HTL(ASCP)QIHC NRH, Norman, OK ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
