RE: [Histonet] Re: Histonet Digest, Vol 102, Issue 26
Hello, I also use Ventana Ultra instruments. We have had our air share of tissue adhesion issues as well. We currently have most of it under control by doing the following: 1. We switched from standard plus slides to Fisherbrand Excel Adhesion slides (cat #22-034-985). I believe they are meant for automated staining, solutions of varying temp and pH, etc. We did see an improvement!! 2. We at all time handle our slides with gloves on. We dip and dry controls upside-down. I was very clear to us that dipping and handling the slide while cutting the control section altered the charge of the rest of the slide. As a rule of thumb...if we need to repeat things due to tissue loss, we run it on a slide by itself and run a separate control. If a focus of tumor is very small or tissue is minimal to begin with, a pathologist may request separate controls right from the start. 3. We also altered some of our processing schedules. At first we were loosing a lot of small biopsies and FNAs. We shortened our processing and now they adhere fine!! 4. We discontinued the use of recycled Xylene in the processor as well. BTW, We routinely cut at four microns, dry vertical for 15 minutes and bake at 60 for 90-120 minutes We have this issue under such a microscope here that we know it will be impossible to get rid of this all together. Breast cases (and sometimes skin) remain to be the only culprits. These tissues just by nature may always be a problem. We now are contemplating running batched controls for ER/PR cases so all those cases are on their own slides. Hope this helps. Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Wednesday, May 23, 2012 12:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 26 I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT Nancy, Your problem is very common, due to fatty tissue is difficult to process. Here's my suggestion if you want to salvage your fatty tissue blocks (presumably the fatty tissues are well fixed); 1) melt down the fatty tissue block re-process 2) cut put on charged slides (Fisher Scientific Ultra Stick charged slides work the best) 3) bake slide for 30 min @ 60c 4) do antigen retrieval with Biocare's Diva for 3 min in Pascal or Biocare pressure cooker (very important; do NOT use Triology Cell Marque pressure cooker). 5) do your ihc as usual Three important elements; (1) well process tissue, (2) Biocare Diva AR buffer, (3) Pascal pressure cooker. Keep us posted what's your outcome. Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - Pathology (histology IPOX) madelein...@elcaminohospital.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health
[Histonet] Excellent opportunity: Histology Manager in Berkeley, CA
Regional Laboratory Section Manager – Histology Company Name: Kaiser Permanente Approximate Salary: Not Specified Location: Berkeley, California Country: United States Industry: Management Position type: Full Time Experience level: 5 years Education level: Bachelor's Degree ID 714 Short Description: Regional Laboratory Section Manager, Histology - Kaiser Permanente in Berkeley, California Long Description: Success is measured one step at a time I hold myself to the highest standards. And believe in my ability to make a difference—one day at a time. As an employee of Kaiser Permanente, I have the freedom to put these beliefs into practice. Here, I have the resources and support I need to realize my potential. If you believe professional empowerment leads to personal satisfaction, this is the place to put your beliefs into practice. We have the following full-time position in Berkeley, California. Regional Laboratory Section Manager – Histology In this role, you will direct and control the day-to-day Regional Laboratory Histology department operations including all personnel activities, technical oversight, and work flow. You will participate in the design of the Regional Laboratory and Northern California Region integrated laboratory quality system; design and implement effective risk control processes; and will ensure compliance with regulatory and accreditation agencies’ rules and regulations. You will also lead in the research of new and/or improved test development methodologies by performing experimental testing procedures; validating effectiveness/feasibility for implementation; cost of procedures; preparing and submitting recommendations for change to laboratory management and other stakeholders (such as Chiefs of Pathologists). You will also participate in department, inter-department, inter-facility, and inter-regional level projects which help the regional laboratory achieve its goal of providing quality service and client support in a cost effective manner. In addition, you will serve as a primary liaison to RILIS/ITS for ongoing and new issues. Qualifications include: • At least five years of experience in a high-volume histology laboratory • Previous supervisory/managerial experience (usually three years) • A bachelor’s degree in biological sciences or a related field; a master’s degree in science is preferred • Additional courses in business administration and/or management preferred • Certification by the American Society for Clinical Pathologists • IHC laboratory operations experience preferred • IHC method optimization/validation experience and IHC instrumentation experience preferred • The ability to work in a Labor/Management Partnership environment We offer a highly competitive salary and an exceptional benefits package. For immediate consideration, please e-mail your resume to charlene.schaef...@kp.org or visit http://jobs.kp.org for complete qualifications and job submission details, referencing job number 133637. Principals only. EOE/AA Employer. If you would like to hear the Kaiser Permanente story as told by our employees, watch the videos at kp.org/jobs/video. Follow us on twitter.com/KPCareers or visit the KP Careers tab on facebook.com/KPThrive. This position supports Kaiser Permanente’s code of conduct and compliance by adhering to all laws and regulations, accreditation and licensure requirements, and internal policies and procedures. jobs.kp.org KAISER PERMANENTE Contact: charlene.schaef...@kp.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
The last person I hired for a tech position, was just finishing her on-line program. I helped her complete that and then she was given two years to get her certification. This was all part of her hiring agreement, so she knew this coming in and completely understood that failing to achieve either one would affect her employment. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 22, 2012 1:43 PM To: sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 22 May 2012 12:39:39 -0500 From: sherrian.mc...@va.gov To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a histotech. I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PVK stain
We are in need of some help trouble shooting this stain. Is it normal for the tissue nuclei to stain red? If not what are we doing wrong? The Chlamydia are staining OK. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
TomI compliment your approach, and I personally really like to see when employers do this. I think it provides opportunity, yet incentive. Two years seems especially reasonable. Most places that I have known who use this method for new-grad hires, have allowed 1 year. It is even better when they provide additional training and support to the person as they are preparing. Having been involved in an online program and clinical curricula in the past, I feel from my observations that continuity with work in the environment is essential for most in connecting the theory with execution. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tpodawi...@lrgh.org To: joellewea...@hotmail.com; sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu Date: Wed, 23 May 2012 10:45:52 -0400 Subject: RE: [Histonet] (no subject) The last person I hired for a tech position, was just finishing her on-line program. I helped her complete that and then she was given two years to get her certification. This was all part of her hiring agreement, so she knew this coming in and completely understood that failing to achieve either one would affect her employment. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 22, 2012 1:43 PM To: sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 22 May 2012 12:39:39 -0500 From: sherrian.mc...@va.gov To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a histotech. I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody titer
When you titer a new antibody you go one below and one above the recommend dilution. If you find that the one above the dilution works do you go then out two more to make sure they don't work? This is a question in our lab right now. Courtney Pierce IHC Specialist Quintiles Translational RD - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pie...@quintiles.com clinical | commercial | consulting | capital ** IMPORTANT--PLEASE READ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HTL exam
We used to have our students take the BOC just before graduation in the summer, but found that the stress of finals coupled with the BOC was a bit much. Especially for those who worked. We do require that the students sign up for the exam (we do this in class as a group) and advise them to take it within about a month of graduation. Some of the students request extra tutoring for the exam and we try to accommodate that as well. Employers that are changing their requirements want registered or eligible; or completion of an accredited program, usually. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org -- Message: 2 Date: Tue, 22 May 2012 12:39:39 -0500 From: McAnn, Sherrian sherrian.mc...@va.gov Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: 61e2b58cecef384094a363989d47c090084e5...@vhav17msga2.v17.med.va.gov Content-Type: text/plain; charset=us-ascii I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a histotech. I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. -- Message: 3 Date: Tue, 22 May 2012 17:42:44 + From: joelle weaver joellewea...@hotmail.com Subject: RE: [Histonet] (no subject) To: sherrian.mc...@va.gov, histonet@lists.utsouthwestern.edu Message-ID: snt135-w565f62180596fc8cfb7f88d8...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 22 May 2012 12:39:39 -0500 From: sherrian.mc...@va.gov To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a histotech. I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 102, Issue 28
Hi, I have done this. It works quite well. It would probably be best to use an alkaline phosphatase detection since the brilliant red of the fast red precipitate is more distinguishable from the brown DAB. (I am assuming you used HRP-DAB detection of the Tunel.) You needn't worry about the left over HRP since you'll be doing an antigen retrieval step which should wash them all out. Amos On Wed, May 23, 2012 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 16 Date: Wed, 23 May 2012 08:03:17 -0700 From: Alicia R. Lange arla...@medicine.nevada.edu Subject: [Histonet] RESTAINING WITH KI67 To: histonet@lists.utsouthwestern.edu Message-ID: 9809822f80b1414c90eb4438713c896801345...@medx.medicine.nevada.edu Content-Type: text/plain; charset=us-ascii Hello Histoland! Has anyone restained tunel slides with ki67? Since tunel stains apoptosis and ki67 proliferation, I was just going to restain with ki67. I wanted to get some input before I did this. Thanks! Alicia Lange ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody titer
Yes, I always want to see the immunoreactivity drop-off (or even disappear) with a higher dilution(s). Otherwise, you might be wasting antibody by using a dilution that is too concentrated. Also, be careful with the recommended dilution. That dilution is only applicable if you are using the exact same staining conditions. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Courtney Pierce courtney.pie...@quintiles.com 5/23/2012 12:57 PM When you titer a new antibody you go one below and one above the recommend dilution. If you find that the one above the dilution works do you go then out two more to make sure they don't work? This is a question in our lab right now. Courtney Pierce IHC Specialist Quintiles Translational RD - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pie...@quintiles.com clinical | commercial | consulting | capital ** IMPORTANT--PLEASE READ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave verification
Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RNA extraction and amplification of LCM rice grain tissue
Dear HistoNet Community, Currently we are working on a procedure to dissect the grain of the rice plant via a PALM Laser Microdisection System. According to published work in the barley grain (Schiebold et al 2011) 2 kits for RNA extraction, purification and amplification were used (for mRNA extraction Dynabeads mRNA DIRECT MicroKit or for total RNA extractionRNeasy MicroKit and for RNA amplification CE Version ExpressArt mRNA amplification Nanokit). We will use the extracted and amplified RNA then for high-throughput RNA Sequencing. Do you have any experience with RNA amplification for very small amount of tissues? Would you use the classical trizol method or should I rely on a commercial kit for extraction? Thanks alot for sharing your knowledge! Best regards, Marlen -- ETH Zürich *Marlen Müller* PhD Student Group of Plantbiotechnology Universitätstrasse 2 8092 Zürich Switzerland ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microwave verification
Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ✓ Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: “Reproducibility” is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ✓ Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ✓ Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ✓ Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, May 23, 2012 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave verification Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
[Histonet] old equipment for parts
I have an old Tissue Tek III cryo console that only gets luke cool and a ThermoFisher slide dryer that died on me. If anyone wants these for parts or boat anchors you can have them, you pay shipping. I hate to trash them if the parts can be recycled. sp Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microwave verification
Ok - attachment didn't work. Whoever wants the procedure, just let me know it is ok to send separately! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 23, 2012 4:27 PM To: 'Bustamante, Lin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microwave verification Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ✓ Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: “Reproducibility” is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ✓ Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ✓ Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ✓ Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, May
[Histonet] RE: Microwave verification
Thank you so much. You are all great. Lin :-) From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Wednesday, May 23, 2012 3:33 PM To: Weems, Joyce K.; Bustamante, Lin; histonet@lists.utsouthwestern.edu Subject: RE: Microwave verification Ok - attachment didn't work. Whoever wants the procedure, just let me know it is ok to send separately! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 23, 2012 4:27 PM To: 'Bustamante, Lin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microwave verification Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ✓ Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: “Reproducibility” is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ✓ Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ✓ Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ✓ Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the
