RE: [Histonet] Slide distribution amongst Pathologists

2012-06-05 Thread Susan.Walzer
Boy, they sure like to put us in the middle of what should be their own 
problem. Thank heavens I only work now for one Dr at a time now but when I was 
at a larger place they rotated. They still used to tell us to give them 
particular cases when it was not their turn so that we got the flak when 
someone did not get what they thought was theirs. You can never win! :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Monday, June 04, 2012 2:34 PM
To: 'Sheila Adey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Slide distribution amongst Pathologists

Here the slides go through the hands of one pathologist, who distributes the
cases. 
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sheila
Adey
Gesendet: Montag, 04. Juni 2012 20:19
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Slide distribution amongst Pathologists


Hello Netters:
I am looking for some ideas regarding slide distribution amongst
pathologists.
Currently one Dr. reads all the surgicals and one other reads the cytology
and bone marrows.
We now have 5 Dr.s and they are looking for ways to disperse the work evenly
per day.
Thanks
:)
 
Sheila Adey
Charge Technologist
Laboratory - Histology Department
Bluewater Health
89 Norman Street
Sarnia, ON  N7T 6S3
519-464-4500 x 7063
 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] View Transit of Venus

2012-06-05 Thread Lee Peggy Wenk
Now for something totally non-histology related – For those of us in the 
basement, who hardly ever see the sun:

Literally a once-in-a-lifetime (about every 112 years) event – Venus will pass 
in front of our Sun tonight Tues June 5. Starting about 5:45 pm Eastern time, 
for about 3 hours. All of North American should be able to see it. (Those in 
other countries – check the NASA website below.)

Do NOT look at the sun directly. Do NOT look at it with regular sunglasses. Use 
welders glass, pin hole camera, or telescope with solar filter are OK. 
Demonstrations of how to do these:
http://www.transitofvenus.org/june2012/eye-safety/281-six-ways-to-see-the-transit-of-venus

Or watch on live NASA broadcast. 
http://sunearthday.nasa.gov/2012/transit/webcast.php

Have fun!

Peggy A. Wenk, HTL(ASCP)SLS
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Slide distribution amongst Pathologists

2012-06-05 Thread Podawiltz, Thomas
We have one simple way of doing it. He who Grosses is he who reads. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Tuesday, June 05, 2012 3:21 AM
To: gu.l...@gmx.at; sa...@hotmail.ca
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide distribution amongst Pathologists

Boy, they sure like to put us in the middle of what should be their own 
problem. Thank heavens I only work now for one Dr at a time now but when I was 
at a larger place they rotated. They still used to tell us to give them 
particular cases when it was not their turn so that we got the flak when 
someone did not get what they thought was theirs. You can never win! :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Monday, June 04, 2012 2:34 PM
To: 'Sheila Adey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Slide distribution amongst Pathologists

Here the slides go through the hands of one pathologist, who distributes the
cases. 
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sheila
Adey
Gesendet: Montag, 04. Juni 2012 20:19
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Slide distribution amongst Pathologists


Hello Netters:
I am looking for some ideas regarding slide distribution amongst
pathologists.
Currently one Dr. reads all the surgicals and one other reads the cytology
and bone marrows.
We now have 5 Dr.s and they are looking for ways to disperse the work evenly
per day.
Thanks
:)
 
Sheila Adey
Charge Technologist
Laboratory - Histology Department
Bluewater Health
89 Norman Street
Sarnia, ON  N7T 6S3
519-464-4500 x 7063
 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential 
information intended only for the use of the recipient(s) named above. If you 
are not the intended recipient, you may not print,distribute, or copy this 
message or any attachments.  If you have received this communication in error, 
please notify the sender by return e-mail and delete this message and any 
attachments from your computer. Any views or opinions expressed are solely 
those of the author and do not necessarily represent those of LRGHealthcare.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Slide distribution amongst Pathologists

2012-06-05 Thread Rathborne, Toni
We do the same.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Tuesday, June 05, 2012 6:06 AM
To: susan.wal...@hcahealthcare.com; gu.l...@gmx.at; sa...@hotmail.ca
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide distribution amongst Pathologists

We have one simple way of doing it. He who Grosses is he who reads. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Tuesday, June 05, 2012 3:21 AM
To: gu.l...@gmx.at; sa...@hotmail.ca
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide distribution amongst Pathologists

Boy, they sure like to put us in the middle of what should be their own 
problem. Thank heavens I only work now for one Dr at a time now but when I was 
at a larger place they rotated. They still used to tell us to give them 
particular cases when it was not their turn so that we got the flak when 
someone did not get what they thought was theirs. You can never win! :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Monday, June 04, 2012 2:34 PM
To: 'Sheila Adey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Slide distribution amongst Pathologists

Here the slides go through the hands of one pathologist, who distributes the 
cases. 
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey
Gesendet: Montag, 04. Juni 2012 20:19
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Slide distribution amongst Pathologists


Hello Netters:
I am looking for some ideas regarding slide distribution amongst pathologists.
Currently one Dr. reads all the surgicals and one other reads the cytology and 
bone marrows.
We now have 5 Dr.s and they are looking for ways to disperse the work evenly 
per day.
Thanks
:)
 
Sheila Adey
Charge Technologist
Laboratory - Histology Department
Bluewater Health
89 Norman Street
Sarnia, ON  N7T 6S3
519-464-4500 x 7063
 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential 
information intended only for the use of the recipient(s) named above. If you 
are not the intended recipient, you may not print,distribute, or copy this 
message or any attachments.  If you have received this communication in error, 
please notify the sender by return e-mail and delete this message and any 
attachments from your computer. Any views or opinions expressed are solely 
those of the author and do not necessarily represent those of LRGHealthcare.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] HPV control slides

2012-06-05 Thread Kelly Colpitts

Happy Tuesday,
We are currently looking for HPV positive control slides for both HPV high risk 
and HPV low risk probes.  Would anyone happen to have a half dozen or so of 
each that they could share?  Please contact me at my email address if you do.  
 
Thanks,
Kelly Colpitts
Nationwide Childrens Hospital
Columbus, OH
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


OT: [Histonet] View Transit of Venus

2012-06-05 Thread Emily Sours
Also, the ISS will be taking close-up pictures of Venus, the first time the
pictures will be very detailed as the cupola wasn't on the ISS before.
http://www.flickr.com/photos/nasa_jsc_photo/7164346718/in/set-72157629649730820/

Emily


You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh



On Tue, Jun 5, 2012 at 6:01 AM, Lee  Peggy Wenk lpw...@sbcglobal.netwrote:

 Now for something totally non-histology related – For those of us in the
 basement, who hardly ever see the sun:

 Literally a once-in-a-lifetime (about every 112 years) event – Venus will
 pass in front of our Sun tonight Tues June 5. Starting about 5:45 pm
 Eastern time, for about 3 hours. All of North American should be able to
 see it. (Those in other countries – check the NASA website below.)

 Do NOT look at the sun directly. Do NOT look at it with regular
 sunglasses. Use welders glass, pin hole camera, or telescope with solar
 filter are OK. Demonstrations of how to do these:

 http://www.transitofvenus.org/june2012/eye-safety/281-six-ways-to-see-the-transit-of-venus

 Or watch on live NASA broadcast.
 http://sunearthday.nasa.gov/2012/transit/webcast.php

 Have fun!

 Peggy A. Wenk, HTL(ASCP)SLS
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Fluorescence-filters

2012-06-05 Thread Gudrun Lang
Hi!

Filters for fluorescencemicroscopy tend to burn out after a certain
duration of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
are about 150 per year.

 

What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised
if the microscope is the culprit and not our protocol. 

 

Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but
tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.

 

Hoping for responses

Gudrun Lang

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] Fluorescence-filters

2012-06-05 Thread Rene J Buesa
Hi Gudrun:
What you wrote is news for me.
Dichroic filters with specific lambda transmission of good quality can be used 
for years.
The filters I used were Ernst Leitz, from the early 1950's and the transmission 
intensity remained strong.
We checked the life duration of the mercury lamp, but not of the filters.
If you are getting weaker signals perhaps is another cause.
René J.



From: Gudrun Lang gu.l...@gmx.at
To: histonet@lists.utsouthwestern.edu 
Sent: Tuesday, June 5, 2012 10:37 AM
Subject: [Histonet] Fluorescence-filters

Hi!

Filters for fluorescencemicroscopy tend to burn out after a certain
duration of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
are about 150 per year.



What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised
if the microscope is the culprit and not our protocol. 



Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but
tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.



Hoping for responses

Gudrun Lang

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Fluorescence-filters -2

2012-06-05 Thread Gudrun Lang

I forgot: the filters are now 10 years old.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Dienstag, 05. Juni 2012 16:38
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Fluorescence-filters

Hi!

Filters for fluorescencemicroscopy tend to burn out after a certain
duration of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
are about 150 per year.

 

What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised
if the microscope is the culprit and not our protocol. 

 

Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but
tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.

 

Hoping for responses

Gudrun Lang

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Fluorescence-filters

2012-06-05 Thread Anatoli Gleiberman
Filters are usually very stable. What is burning out are fiber optic cables 
that are present in some illumination systems such as X-Cite in Zeiss 
microscopes. These optical fiber cables should be replaced after 2000 hours.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, June 05, 2012 10:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fluorescence-filters

Hi!

Filters for fluorescencemicroscopy tend to burn out after a certain duration 
of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are 
about 150 per year.

 

What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised if 
the microscope is the culprit and not our protocol. 

 

Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but 
tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.

 

Hoping for responses

Gudrun Lang

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


This communication may contain privileged information.  It is intended solely 
for the use of the addressee.  If you are not the intended recipient, you are 
strictly prohibited from disclosing, copying, distributing or using any of this 
information.  If you received this communication in error, please contact the 
sender immediately and destroy the material in its entirety, whether electronic 
or hard copy.  This communication may contain nonpublic information about 
individuals and businesses subject to the restrictions of the 
Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose 
such information for any purpose other than to provide the services for which 
you are receiving the information.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Reagent rotation

2012-06-05 Thread Mark Perrin
What is the normal procedure for changing the reagents in the tissue processor? 
We have a Sakura VIP6. Should the schedule of changing be based on number of 
runs vs. number of blocks processed? About 80% of our blocks are small GI 
biospies.

Thanks in advance for your input.

 
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Reagent rotation

2012-06-05 Thread Goins, Tresa
We rotate by cassette number - our daily load can vary from 10 to more than 100 
cassettes, so an exchange schedule based on number of runs was not appropriate.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 12:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? 
We have a Sakura VIP6. Should the schedule of changing be based on number of 
runs vs. number of blocks processed? About 80% of our blocks are small GI 
biospies.

Thanks in advance for your input.

 
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Reagent rotation

2012-06-05 Thread Stephenson, Sheryl

We do it by the number of Runs.  About every 6 run for rotations and 12 runs 
for a full change out.   But we also have another processor as well.

Sheryl Stephenson | Histology Technician 

   Main 908.947.1100 Fax908.947.1085
 Direct:  908.947.1624 sstephen...@lifecell.com
 732. 939. 3037  Cell www.lifecell.com  

LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876


 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 2:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? 
We have a Sakura VIP6. Should the schedule of changing be based on number of 
runs vs. number of blocks processed? About 80% of our blocks are small GI 
biospies.

Thanks in advance for your input.

 
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Current Histology Openings

2012-06-05 Thread Brannon Owens
Allied Search Partners is seeking qualified candidates for the following
openings in Histology.
1)  Night time Histology Supervisor- North of Los Angeles, CA
2)  Histology Manager- Fort Myers, FL
3)  Part time Mohs Tech- Denver,  CO
4)  IHC Technologist- Port Chester, NY

Email/message me for full job descriptions!
-- 
Brannon Owens
Recruitment Manager
Allied Search Partners


T: 888.388.7571 ext. 106

F: 888.388.7572




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] Fluorescence-filters

2012-06-05 Thread Mark Tarango
Hi Gudrun,

i read about 15 ALK cases a week.  If you are seeing a lot of collagen
fibers around the tumor cells, I'd try increasing the digestion time of
your pepsin (especially if they were fixed for longer than usual).  Before
altering the pretreatment though, you would want to make sure that these
slides were not exposed to light for any period of time.  The discrete
signals of the probes can quickly fade (much faster than with IF stained
slides).  I'd also make sure the door is closed in your dark room.  If
there is light in the corner of your eye the signals can be hard to see.
 ALK FISH should be scored at high power under oil immersion (60-100x
objective).  If they are scoring at 40x, it could be a problem.

good luck!

Mark Tarango

On Tuesday, June 5, 2012, Eric Hoy wrote:

 We do a LOT of fluorescent microscopy in our immunology lab, so I have a
 bit
 of experience with fluorescence.

 The answer to your question depends on what type of filters you have in
 your
 microscope, and what type of light source is on the microscope.

 Older fluorescent systems used absorption filters, which were simply discs
 of coloured glass.  These filters had a fairly wide band-pass, so the
 fluorescence tended to be less than we see with interference filters.  The
 good news with these filters is that they are nearly indestructible (unless
 you drop and break them.)

 Interference filters are produced by vacuum deposition of a thin film of
 metal vapour on high-quality glass.  These filters usually have much
 sharper
 band pass characteristics than absorption filters.  They are also
 considerably more expensive.  If handled properly, these filters will last
 for decades, but improper cleaning and handling of the filters can shorten
 their lifespan.  I have also heard that prolonged exposure to solvent
 vapours (such as we find in a histology lab), can damage the filters,
 although I have not seen any filters that suffered this type of damage.

 I have seen interference filters that show delamination of the metal film
 over time.  In my experience these are older filters that were not produced
 with the current technologies, and filters that have been mishandled.
 Interference filters made in the past 20 years should last as long as the
 microscope, if they are properly handled.

 If you are seeing reduced fluorescence, I would suspect the light source as
 the most likely problem.  Halogen lamps have less intensity than mercury
 vapour lamps, which are less intense than metal halide lamps, which are
 less
 intense than LED sources.  We have converted all of our microscopes to LED
 sources.  If you are using an older HBO or halogen lamp, the age of the
 lamp, the initial wattage of the lamp, and the alignment can all affect the
 fluorescent output.

 As you identified, perhaps the most important aspect of immunofluorescence
 is the skill and experience of the person who reads the slides.

 Let me know if you have further questions.

 Eric Hoy

 ===
 Eric S. Hoy, Ph.D., SI(ASCP)
 Clinical Associate Professor
 Department of Medical Laboratory Sciences
 The University of Texas Southwestern Medical Center
 Dallas, Texas
 Email: eric@utsouthwestern.edu
 ===

 On 6/5/12 9:37 AM, Gudrun Lang gu.l...@gmx.at javascript:; wrote:

  Hi!
 
  Filters for fluorescencemicroscopy tend to burn out after a certain
  duration of usage. What duration?
 
  We have filters for FITC, TRITC, Dapi and a triplefilter. The
 working-hours
  are about 150 per year.
 
 
 
  What do you think? Is it time to change them.
 
  I have often bad feedback about weak signals, and I would not be
 surprised
  if the microscope is the culprit and not our protocol.
 
 
 
  Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed
 but
  tumourcells mixed within collagenfibers.
 
  - and unfortunately unexperienced doctors on reading of this special
 probe.



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu javascript:;
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] NEW Position Alert - Quality Assurance Histologist

2012-06-05 Thread Matt Ward
Good Evening Histonet,



We are currently searching for a Histotech that would be interested in a QA
role with a global leader in Histology based in Richmond IL. The company is
growing and offers a strong package.



Please respond to this e-mail with your resume if you would be interested
in learning more.



*Quality Assurance Histologist *





*The Company:*



Well-established provider of consumables and medical device accessories for
clinical histology and research laboratories.  The facility works closely
with our UK, German and Australian facilities in the development,
manufacturing and marketing of products including processing reagents,
storage and specimen transport devices, cytology accessories and safety
products.



This is a globally focused business with significant sales and operations
in the US, Europe and Asia Pacific as well as a direct presence in over 100
countries.



*The Opportunity:*



The company currently has an opening for a Quality Assurance Histologist to
be based in Richmond IL. This position reports to the Quality Assurance
Manager.  All applicants must not be adverse to travel, as this is a
position that may require domestic and international travel when necessary.



Salary: Commensurate with experience



Other: Full benefits - 401k program/matching



*Primary Responsibilities: *



The primary purpose for this position is to perform product specification
and functionality testing on incoming raw material and final products to
release for distribution.



Additional Responsibilities:

- Perform incoming, in process and final inspection on product for release

- Effectively communicate the status of product testing

- Assist in the maintenance of laboratory facilities, equipment and
consumables

- Perform additional testing and investigation related to customer
complaints

- Maintain laboratory equipment

- Good documentation practices

- Necessary computer skills, Microsoft Word, Excel, and Powerpoint. Use of
SAP is preferable

- A commitment to follow standard company financial procedures

- Achieve best practice safety performance levels

- Product training and quality systems training



*Education and Experience Required:*



- Ability to work independently and as part of a team

- Able to perform tissue grossing, tissue processing, embedding

- Sectioning paraffin embedded tissue as well as frozen tissue

- Performing routine stains (H and E) as well as special stains

- Formulation and production of routine laboratory reagents and solutions

- Performing and documenting routine laboratory procedures

- Familiarity with compliance requirements in the medical device industry

- Good communication skills both verbal and written

- Proficiency in basic computer skills and with software applications such
as Microsoft Office



Regards,



Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] Fluorescence-filters

2012-06-05 Thread Emily Sours
Wow, that was detailed and interesting!!
I didn't know microscopes could even use LED, does that require a different
setup, or just a different bulb?

Emily

You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] Fluorescence-filters

2012-06-05 Thread Emily Sours
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).

Emily

You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Reagent rotation

2012-06-05 Thread Ian R Bernard
We do it by number of blocks- At or after 350 blocks the processor is changed.

Ian

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephenson, 
Sheryl
Sent: Tuesday, June 05, 2012 2:03 PM
To: 'Mark Perrin'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation


We do it by the number of Runs.  About every 6 run for rotations and 12 runs 
for a full change out.   But we also have another processor as well.

Sheryl Stephenson | Histology Technician 

   Main 908.947.1100 Fax908.947.1085
 Direct:  908.947.1624 sstephen...@lifecell.com
 732. 939. 3037  Cell www.lifecell.com  

LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876


 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 2:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? 
We have a Sakura VIP6. Should the schedule of changing be based on number of 
runs vs. number of blocks processed? About 80% of our blocks are small GI 
biospies.

Thanks in advance for your input.

 
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet