[Histonet] Hologic Imaging System
Hello all. For anyone who is using the Hologic Imaging System for Cytology I was wondering about the pre-analytical steps involved. I would appreciate any thoughts you could share regarding the process of preparation and scanning. Equipment, space requirements, time, labeling/barcoding, etc. Thanks so much! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Derm lab
Hi everyone! I was wondering if anyone knows the typical protocol for skin that ask for a PAS staining procedure on the requisition form. Thanks! Hope everyone has a nice day! Danny Zapata Dr.Norman Dermatology Tampa, Fl Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for the thoughts on Sequenza units!
Hello All, I have compiled all of the responses to date regarding the question I posted on histonet about too much volume and reverse capillary action: (I've changed font styles to differentiate between responses) I have used them for quite some time. I was having problems with uneven staining and determined that it was from the being too full and was drawing up liquid from the bottom. There is a small opening on the end and I just empty the solution from it. I usually empty it after rinsing the antibody off and then usually before putting DAB or DAPI on depending on the number of slides stained. As for washing, I just wash in a solution of soapy water and then rinse in distilled. I use the coverplates for several staining times. I love the sequenza and prefer it to having an automatic stainer. I still use these and have 9 racks. The coverplates can be found on eBay, but I clean them in soapy bleach water, rinse well, and dry until the plastic gets brittle and they break. I use AEC 99% of the time so I do not generate the waste of DAB. If the racks are filing up with reagents and solutions, leave an empty slot and pipet it out. Otherwise, I dump it and and rinse after the run. For the antibodies and detection solutions, you only need 3-4 drops. I only fill the well to the top with rinses. We use these units, I have never had an issue with the waste reagents touching the bottom of the plates. We never use more than 3 drops of kit items, the wells are filled with buffer. It never seems to be alot of waste. After we finish staining we just rinse the holders out with running water for a few minutes and let air dry. We empty them before they get to that stage, as this would be bound to affect the efficient working of the Coverplates. This is Tyler Liebig with the Thermo Scientific IHC group. I'm responding to Keri's questions regarding the Sequenza manual staining. Keri you are correct that if the waste is not emptied and builds up too much in the bottom of the sequenza unit it will definitely stop the capillary action (Reagent can't flow both ways :-). However, this can be avoided by emptying the waste regularly between runs. You bring up a good question though that I didn't know the answer to: How many slides can be stained before the waste hits the slides? I did a quick calculation below that I hope is helpful. Total Waste Volume of Sequenza Rack: In a Sequenza rack the slides are held about 1.5 inches above the bottom of the tray. This allows about 270ml of waste before the level is close touching the slides. Reagent use per slide: If you stain a single slide with a long protocol then worst case scenario is about 7 steps. (H2O2 through Counterstain with a two step polymer) Volume of rinse buffer: This means you would rinse 8 times 2ml each (16ml total rinse buffer per slide worst case). For the staining reagents: The recommended volume is 0.1ml per reagent and 0.5ml for chromogen. So for 6 reagents plus chromogen that is 1.1ml and I will double it because I know some people add way more than needed. So 2.2ml per slide worst case. So to sum it up worst case scenario 18.2ml of waste is created per slide which means 14 slides (270/ml/18.2ml=14.8) can be stained before the waste risks touching the slides. The rack holds ten slides total. In the end I recommend users play is save and empty the waste after each batch of slides stained even though most user generate much less waste than the calculations above. I also included a link to a great description of Sequenza use on the Histonet posted by Gayle M Callis HTL/HT/MT (ASCP). I have referenced these details frequently myself (Thanks Gayle). http://www.histosearch.com/histonet/Jul02A/Longansweronusetechnicswi.html I have also read through Gayle's post on Histonet, it is worth reading through for some more insight on the use of these units. Keri ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Urate crystals
We have a piece of bone that the pathologist would like to process for urate crystals. The procedure we have is De Galantha's, which calls for decalcification by nitric acid, which we don't have. What other decalcification process can be used? Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing IHC on MOHS
I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorre...@kmcpa.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing IHC on MOHS
If each location is identified as a separate specimen, you can bill per specimen, is the way I understand it. e.g. Received separately - 88342 x 4 3:00 margin - A 6:00 margin - B 9:00 margin - C 12:00 margin - D If one specimen is received and divided into separate cassettes - 88342 x 1 A1 - 3:00 margin A2 - 6:00 margin A3 - 9:00 margin A4 - 12:00 margin Best, $1,783.00 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Torrence Sent: Thursday, June 14, 2012 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing IHC on MOHS I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorre...@kmcpa.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing IHC on MOHS
Is this per Specimen or per surgical site. Mohs specimens are usually cut into smaller pieces and inked after the excision is removed. I would think this constitutes x# of blocks from the same specimen. Same as an excision that is breadloafed into separate sections. I don't think it matters who does the cutting, although when we send these to path for permenents they are logged in a separate specimens but only measured, never breadloafed. Is it more dependent on how it is received in the lab even though the end result is still the same? Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thu 6/14/2012 2:20 PM To: 'Carol Torrence'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing IHC on MOHS If each location is identified as a separate specimen, you can bill per specimen, is the way I understand it. e.g. Received separately - 88342 x 4 3:00 margin - A 6:00 margin - B 9:00 margin - C 12:00 margin - D If one specimen is received and divided into separate cassettes - 88342 x 1 A1 - 3:00 margin A2 - 6:00 margin A3 - 9:00 margin A4 - 12:00 margin Best, $1,783.00 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Torrence Sent: Thursday, June 14, 2012 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing IHC on MOHS I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorre...@kmcpa.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASCP HT exam
Hello there histonetters. Some of you may remember some previous posts I've put up in regards to employment and taking the HT exam. I sat for my exam on Monday and..drum roll please I PASSED :):):):) I'm on the move to the bay area specifically Berkeley, and will be there by Sunday. So any info on jobs in that area would be greatly appreciated!!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bleach the carbons pigments
Hi All, One of our pathologists wants to bleach the carbons pigments from cytology smears.According to Bancroft (page 263) it may be confused with melanin deposition but treatment with bleaching agents will show carbon unaffected. Is there any procedure? Muhammad Tahseen Senior Supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
