[Histonet] FW: bone regrowth
From: Molinari, Betsy Sent: Thursday, June 28, 2012 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: bone regrowth Hi all, I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences. It involves bone regrowth. The sample will vary in size. From my reading, it seems that 5% formic or 10% EDTA is preferred. The researcher asked about acetic acid, but I have never heard as acetic alone as a decal, isn’t it just used to balance pH of the EDTA? It is my understanding that the decal step will be done somewhere else then the sample sent to me. That lab feels that HCL decal will not not affect the sample. I think otherwise. The staining they have talked about is the Goldners Trichrome mainly. The researcher mentioned Safranin O but from my conversations I think they have little knowledge of histology and I work in a cardiovascular lab with my only experience has been with mouse tibia and these samples will be from a larger source. Type 2 collagen was brought up but when I said that was an immuno stain they did not realize that. Si in a nutshell, what decal sol. would be better and what stain? Sorry for the rambling. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Veterinary histology job at UF
Hello Histonetters, The Anatomic Pathology Service within the University of Florida, Veterinary Diagnostic Laboratories (College of Veterinary Medicine) is recruiting for a Laboratory Technician in the Histology Laboratory. The work involves diagnostic veterinary histology and research histology. Full time, 40 hours per week, days, no weekends. The HT (ASCP) is not a requirement for employment. We also do not require the Florida State Laboratory License. A high school diploma and two years of appropriate experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience. Veterinary histology experience a plus. Willing to train the right person. The University of Florida offers a generous benefits package, including one of the best pension plans in the State. Gainesville is a beautiful college town, with large oaks and nearby spring fed rivers for canoeing or kayaking. Housing is very affordable. The job listing can be viewed at: https://jobs.ufl.edu/ Job title: Laboratory Technician job requisition number is 0900939 Sarahhttp://www.ufl.edu/ A. Jones, HT (ASCP) Histology Lab Manager sarahjo...@ufl.edumailto:sarahjo...@ufl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hydrometer questions
Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gross macro digital camera
We are using a Canon G12 for macros. This is actually our second one. We are very pleased with it. Images are really good. Our Pathology software is PowerPath. Good luck! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of b427...@aol.com Sent: Thursday, June 28, 2012 11:40 AM To: Marc DeCarlo Cc: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross macro digital camera Would appreciate suggestions for a good digital camera for taking macro photos during necropsy. Any takers? Thanks much. Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hydrometer questions
Tim: When I (and others) wrote you about the age of the LFB I at least was referring to the age of the solution. You write now that you are going to try a 1950 LFB and I am sure that you are referring to the powder. As to powdered stains they are quite stable, but it will be nice to find out. To your hydrometer question: they are very simple to use, you just place the instrument very delicately into the ethanol sol. contained on a cylinder and it will sink up to a mark on the stem corresponding to the ethanol concentration, BUT there are some precautins to take: 1- the density of ethanol (and of any liquid for that matter) varies with the temperature; to higher temperature less push of the liquid and if the temp. is high you will get a reading corresponding to a higher ethanol concentration that the real value. 2- the cylinder has to be wide enough, otherwise the stem may come close to the wall of the cylinder and give you also a false reading. Your best solution is to prepare fresh ethanol sol. every time you need it and to do that you just calculate how much dist. water you need to add to your ethanol of KNOWN concentration. Finally, if you get a hydrometer for ethanol it cannot be used for isopropyl alcohol, because each has a different density. Hydrometers can be bought from Fisher or Thomas Adams (I think they still sell them!). René J. From: Tim Wheelock twheel...@mclean.harvard.edu To: histonet@lists.utsouthwestern.edu Sent: Friday, June 29, 2012 10:08 AM Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hydrometer temperature correction
There is a chart [ http://www.defalcos.com/tutorials/hy.html ] for temperature correction on ethanol concentration. A lot of information can be found on brewery sites. So . . if you require more help, and since its Friday, visit a local brew house to ask questions. Tresa :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, June 29, 2012 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paperless Histology
I have done this in a previous lab by going to a LIS with bar coding throughout the lab. It is not a quick and easy thing to do, but well worth it. I don't know of another way to do it. We are currently going the same route where I'm working now. It can be a long and tedious project - but again, well worth the work to get there. Lee -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paperless Histology
Hello Stella, My cousin is an Account Executive for an EMR (Electronic Medical Records) Company, and I can gladly put you in touch with him if that is the sort of paperless option that your Director seeks. Please let me know if I can be of any assistance, Noah Noah Rogers Sr. Recruiter | Maxim Healthcare Services DC/MD/VA - Allied Staffing Division 9525 Georgia Ave | Suite 205 Silver Spring, MD 20910 301.755.2089 (P) 877.748.8793 (F) 703.597.9186 (C) Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. Confidential Health Information Attached. Health care information is personal and sensitive. It is being faxed/emailed to you after appropriate authorization from the patient/employee or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain this information in a safe, secure and confidential manner. Re-disclosure without additional patient consent or authorization or as permitted by law is prohibited. Unauthorized re-disclosure or failure to maintain the confidentiality of this information could subject you to penalties under Federal and/or State law. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Managerial Job Opening in Houston
Hi Everyone, I hope everyone out there is having a happy Friday. I wanted to let anyone know, who may be interested, that I have a permanent Histology Manager job in Houston, TX open right now. Please message me for the details and job description if you think you might be interested. Looking forward to hearing from some of you! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paperless Histology
StellaI have worked at a couple labs that were basically paperless. The papers that they did have were more or less just hard copies for records since some people were not entirely trusting of an entirely electronic system. This was a while ago, and the choices from an IT perspective are surely much better now. Overall, I think it worked pretty well and did save alot of time ( and trees!), and did not have any more glitches than any paper based process I have worked in once it got up and running. I am sure any/all vendors of workflow management and LIS applications could provide you with details and options. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Fri, 29 Jun 2012 10:25:27 -0500 From: estellamire...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Needing a control block please
Hi everyone - Does anyone have an appendicitis case where they can send me a couple of control blocks? I need a good fibrin control block. If you can help out, please let me know and I'll give you my mailing address. Thanks! Kristie Kristie This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT/HTL Opening Near Binghamton, NY
Allied Search Partners is currently looking for an outstanding Histotech to join a dynamic organization in New York. Location: Binghamton, NY Schedule: Full Time Day Shift To Apply: Please send resume to bran...@alliedsearchpartners.com or fax to 888 388 7572. Please note that no other information is given at this time about location or organization. Summary and Job Requirements: · Small Private Laboratory · HT or HTL ASCP Certification Required · New York Licensed · Must meet CLIA requirements to Gross · Grossing Experience · Full Healthcare Benefits Please submit resume via email or fax and if you meet requirements one of our recruiters will give you a call. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paperless Histology
And what about a paperless procedure manual? We have an electronic system, called Proquis, for our manuals. It sends an e-mail to all the appropriate people, when there is a new procedure to review or when old procedures are due for review. Pretty cool. The only drawback is, most of the time, people would rather grab a notebook to look up a procedure, rather than the cumbersome process of logging in, clicking thru a bunch of menus to view what you could have physically touched and seen in seconds. Plus, you better have a lot of terminals. Or better yet, let's issue I-Pads to all employees. ;-) We live in a brave new world! Sandy H. Histology Supervisor, VA Minneapolis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: bone regrowth
Betsy I would use formic acid rather than EDTA, especially if you need to run collagen II IHC, we ran into an issue with a study that was decaled in EDTA and the collagen II IHC was not as optimal with the EDTA decal, formic acid is much better for collagen II. We also get good results for safranin O in most samples (except mouse joints) decaled in formic acid. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, June 29, 2012 6:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: bone regrowth From: Molinari, Betsy Sent: Thursday, June 28, 2012 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: bone regrowth Hi all, I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences. It involves bone regrowth. The sample will vary in size. From my reading, it seems that 5% formic or 10% EDTA is preferred. The researcher asked about acetic acid, but I have never heard as acetic alone as a decal, isn't it just used to balance pH of the EDTA? It is my understanding that the decal step will be done somewhere else then the sample sent to me. That lab feels that HCL decal will not not affect the sample. I think otherwise. The staining they have talked about is the Goldners Trichrome mainly. The researcher mentioned Safranin O but from my conversations I think they have little knowledge of histology and I work in a cardiovascular lab with my only experience has been with mouse tibia and these samples will be from a larger source. Type 2 collagen was brought up but when I said that was an immuno stain they did not realize that. Si in a nutshell, what decal sol. would be better and what stain? Sorry for the rambling. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
