Re: [Histonet] What is this item called?
Hi Adam, The photo was helpful. I've used them before in an embryology lab, for dissecting out Drosophila imaginal discs. We had single-well and 3-well versions. The 3 well version was slightly longer than the one you showed, and the wells were smaller, but the general proportions were the same (gently sloping sides, not steep slides like a spot plate). I did some searching because I'd like to have a few for our lab too. The best I could come up with is: 1 - Corning and Fisher Scientific used to carry 3-well versions but both are now discontinued. Corning #7223. Fisher #21-379. 2 - Fisher also carried single well versions (referred to in a number of Drosophila dissection protocols), but no product # mentioned. 3 - these are similar, but the depression is not sloped: http://www.leaveonlybubbles.com/catalog/product_info.php?products_id=63 4 - Not sure if you are using glass or ceramic spot plates, but there are glass spot plates with more gradually sloping sides that might fit your needs: http://lab-crunch.ecrater.com/p/11132699/corning-pyrex-9-well-spot-plate http://www.mccronemicroscopes.com/store/catalog.asp?item=345category_id=0 You might try sending your photo to Fisher/Corning etc customer service and see what they come up with. Let me know if you find anything. Good luck! Andrea Andrea Marion Graduate Student University of Illinois at Chicago ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Methanol in H2O2 explanation
I thought that I answered the original Q? Imho, 3% H2O2 in distilled water is as good as any other recipe, given time and concentration. Sure, use a lower conc IF you achieve negative rbcs. I use water because it is cheaper. A lower concentration will take longerwhy bother?? Kill that enzyme. If you are concerned re frozen sectionswell, use Glucose oxidase system. Far more expensive. Good in some very messy bloody situations... Non-reversible ( irreversible) denaturation/inactivation of endogenous Pxs is achieved by feeding them a massive overdose of H2O2. This is not suicide...it's murder! Well, depends on your definition of Life ;-) NB: inactivation can be reversible/non-reversible so, imho, not a good term to use. Use irreversible inactivation? Them peroxidases have evolved over millions of years to survive and...we come along with hair bleach and kill them! Re methanol/H2O2 adversely affecting Pwax sections: I say no way. The tissue has been processed via alcohols for several Hrs. Bottom line: Water/Methanol/azide/H2O2.are your rbcs/neutrophils colourless ?? Have you got a good section? If yes, then your H2O2 blocking method is OK!! So...if you use an expensive commercial H2O2 blocking reagent...why? To be sure.I completely subscribe to the Skool that says that if you do get some non-specific staining but, get very good specific stainingignore the former. Eg: if you get rbc staining with a GFAP Abso what? It is only aesthetics. Understand what you are doing and...why. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Methanol in H2O2 explanation
If you have tissue that has more peroxidase than usual, then extra time is required. Depends on the concentration of H2O2 used ;-) A massive excess of substrate ( 3%) is good. Carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Training
Hi, I have a question about the training requirement for the HT Certification Test. My question is, will the ASCP allow a guy who works as a lab assistant in the histology lab andintends to train as an histotech for one and half hour a day for twelve months to sit for the test? Thanks, Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fo a small GI lab.... Question
How do you store your waste before been picked up by any company. I need to know what is acceptable by CAP Inspectors; temperature for the room. Our containers are outside lab, etc. Any information will be very much appreciated. Thank you. Lin Bustamante ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] fo a small GI lab.... Question
We have a metal drum, 55 gallon that is stored at rm temp. We have a weekly check that we document. Sent from my iPhone On Jul 14, 2012, at 4:14 PM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: How do you store your waste before been picked up by any company. I need to know what is acceptable by CAP Inspectors; temperature for the room. Our containers are outside lab, etc. Any information will be very much appreciated. Thank you. Lin Bustamante ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
