[Histonet] RE: Teabags
I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like them because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Monday, August 06, 2012 10:37 AM To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Teabags I don't know if this would work for everyone, but we process our hair with skin scraping samples (animal source) in lens paper. We form a small packet with all folds on one side of the packet and embed the entire thing with the one layer of lens paper down. Keeps everything corralled so to speak. Tresa Goins Montana Veterinary Diagnostic Lab Bozeman, Montana 59718 406-994-6353 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Monday, August 06, 2012 8:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Teabags (Contact HistoCare) You can use teabags to give specimens shape such as EMB and ECC. Those lose particles may not wash through a microcassette, but would just be loose pieces in there if not in a bag. You try to pick up those tiny pieces out of the corners. In a bag you can scrape them off, shape them and put them in the mold. Small individual samples such as GI biopsies can go in a biopsy cassette. Great question, I am going to add it to my exam for my students. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!
The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You wont want to miss out on this opportunity to learn about and witness first hand all current forms of resin/plastics histology, as well as see for the first time ever here in North America a newly developed non-contact laser microtome! 7:30am - Registration Opens 8:00am – Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique – Jack L Ratliff 9:00am – Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It – Daniel S Perrien, Ph.D. 10:30am – Refreshment Break 10:45am – Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome – Damien Laudier, BS, HTL(ASCP), QIHC 12:15pm – Lunch On Your Own 1:15pm – Ground Section Microtomy Techniques (Parts I II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens – (PART I) Joe Tabeling Jack L Ratliff, BA; (PART II) Linda Durbin Robert A Skinner 2:45pm – Refreshment Break 3:00pm – New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses – Heiko Richter, Ph.D. 4:00pm – Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy – Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack RatliffChairman, Hard Tissue Committee - National Society for Histotechnology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Portable Clean Air System
All ~ Just curious if anyone has any experience with the Airfiltronix portable fume extractor filter? I saw it in the Ted Pella catalog, item # 3120, http://www.tedpella.com/safety_html/3120.htm It is designed to be used with a microwave, but can be used anywhere, according to the catalog. We are thinking about using something like this in our processing lab due to space constraints. Any input is greatly appreciated. Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: opinion on heating slides prior to IHC
Dear J, I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process. I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it. As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to say they might have had to try multiple antibodies to find one that would work under those conditions? The only way to know if heat or other conditions will negatively affect your target protein is to test it empirically. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome?
Hi Guys Does anyone have a copy of the manual for the Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? Thanks in advance. Jennifer L. Johnson, Ph.D. Staff Scientist The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037 jjohn...@scripps.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Teabags
Susan Walzer notes I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Teabags
I use hair perm papers that I buy from a local beauty supply store. WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Teabags
For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5 x 4 sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclo...@wellspan.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decalcification of premolar teeth (dog)
Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!!
Greetings! The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You won't want to miss out on this opportunity to learn about and witness via LIVE demonstration all current forms of resin/plastics histology equipment. A special treat this year will be a first ever showing and demonstration in North America of a newly developed non-contact laser microtome (TissueSurgeon) to cut micron thin sections of a variety of tissue types! 7:30am - Registration Opens 8:00am – Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique – Jack L Ratliff 9:00am – Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It – Daniel S Perrien, Ph.D. 10:30am – Refreshment Break 10:45am – Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome – Damien Laudier, BS, HTL(ASCP), QIHC 12:15pm – Lunch On Your Own 1:15pm – Ground Section Microtomy Techniques (Parts I II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens – (PART I) Joe Tabeling Jack L Ratliff, BA; (PART II) Linda Durbin Robert A Skinner 2:45pm – Refreshment Break 3:00pm – New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses – Heiko Richter, Ph.D. 4:00pm – Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy – Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] help ! paraffin section
Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
