[Histonet] slide dryer
Looking for a small slide dryer, used preferably, for our School of Histotechnology. Not a lot of counter space. Only 6 students, so don’t have a lot of racks at any given time. We have an old Shandon-Lipshaw rectangle metal “box”slide dryer right now, but the insulation is going on it, so we need to get a new slide dryer. The drying chamber is about 10” x 7”, with the motor/heater towards the back after that. Do NOT have the money or space for one of the new “round” slide dryers with the see through top. Do NOT want a slide warmer that looks like a long hot plate, as we have too many slides all at once for that. Vendors, or anyone with an idea of where I can get a small rectangular slide dryer – please contact me at work at pw...@beaumont.edu Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Zinc-Tris mouse hearts
Hi, I am going to have some mouse hearts that have been fixed in zinc-Tris fixative. I have never worked with this before. My question is about processing. After doing some looking, I found an article that advised checking the solutions on the processor , the first couple of alcohols , to be sure there is no carry over of formalin. I understand that the object if this fixative is to avoid formalin but is this step necessary? Thank you in advance. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tossing old tissues...
Hello all, A question came up during our last JCAHO inspection regarding the dumping of old tissues. Do you just throw out the intact specimen containers with formalin and all into biohazard trash? Do you drain/collect the formalin for other disposal and just place the drained tissues into the biohazard trash? If you drain/collect, do you wear a respirator while doing it? We have always drained and only thrown the tissues into the bio trash and have not used a respirator. We monitor regularly and always make certain to include this task (along with the making of formalin) when monitoring for exposure and are always way below the acceptable limits. I don't think we have ever done and STEL just for the dumping of tissues though. Just wondered what everyone else did. I would like to stop draining and just dispose of container and all but we pay by the pound for disposal. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tossing old tissues...
We put everything in double red bags and add packets of Neutralex to equal approximate amount needed. We do empty large containers, but I'm thinking about stopping that as well. We have Chemclav on site and have no known problems for the past several years. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, September 11, 2012 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tossing old tissues... Hello all, A question came up during our last JCAHO inspection regarding the dumping of old tissues. Do you just throw out the intact specimen containers with formalin and all into biohazard trash? Do you drain/collect the formalin for other disposal and just place the drained tissues into the biohazard trash? If you drain/collect, do you wear a respirator while doing it? We have always drained and only thrown the tissues into the bio trash and have not used a respirator. We monitor regularly and always make certain to include this task (along with the making of formalin) when monitoring for exposure and are always way below the acceptable limits. I don't think we have ever done and STEL just for the dumping of tissues though. Just wondered what everyone else did. I would like to stop draining and just dispose of container and all but we pay by the pound for disposal. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hair mount
Dose anyone have a hair mount procedure they would like to share? This is unprocessed hair that is mounted on slides. After time we get some major airbubbles and thought of trying nairl polish around the edges, but thought I would ask out in histoworld to see what you all are doing. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] air drying special stain slides rather than dehydrate and clear
I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] blocks and slides
What is the number of years that you must keep blocks and slides for future reference according to CAP and/or Joint Commission? I have heard 5 years for blks, and 10 years for slides. Dorothy R. Glass, BS,HTL(ASCP),IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blocks and slides
Most states have their own regulations. I kept the blocks for 9 years and the slides for-ever but after 9 years they were sent to a company that kept them for us. René J. From: Dorothy Glass techman...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 11:09 AM Subject: [Histonet] blocks and slides What is the number of years that you must keep blocks and slides for future reference according to CAP and/or Joint Commission? I have heard 5 years for blks, and 10 years for slides. Dorothy R. Glass, BS,HTL(ASCP),IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] blocks and slides
Dorothy, We are required by CAP to keep both our blocks and slides for 10 years. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Tuesday, September 11, 2012 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blocks and slides What is the number of years that you must keep blocks and slides for future reference according to CAP and/or Joint Commission? I have heard 5 years for blks, and 10 years for slides. Dorothy R. Glass, BS,HTL(ASCP),IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] blocks and slides
CAP states 10 years for both slides and blocks. ANP.12500 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Tuesday, September 11, 2012 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blocks and slides What is the number of years that you must keep blocks and slides for future reference according to CAP and/or Joint Commission? I have heard 5 years for blks, and 10 years for slides. Dorothy R. Glass, BS,HTL(ASCP),IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] blocks and slides
Hey Dorothy, It's 10 years for CAP, but you need to check with your state as well. I know for New York you must hold them for 20 years. Thanks, Tim Message: 20 Date: Tue, 11 Sep 2012 08:09:24 -0700 (PDT) From: Dorothy Glass techman...@yahoo.com Subject: [Histonet] blocks and slides To: histonet@lists.utsouthwestern.edu Message-ID: 1347376164.71177.yahoomailclas...@web114504.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 What is the number of years that you must keep blocks and slides for future reference according to CAP and/or Joint Commission? I have heard 5 years for blks, and 10 years for slides. Dorothy R. Glass, BS,HTL(ASCP),IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: air drying special stain slides rather than
Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrate and clear To: histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain; charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana -- Message: 17 Date: Tue, 11 Sep 2012 07:52:05 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] air drying special stain slides rather than dehydrate and clear To: Diana McCaig dmcc...@ckha.on.ca, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1347375125.72189.yahoomail...@web121405.mail.ne1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Diana: The most simple answer to your question is: Because that is the way it has been done for more than 150 years. The second question would be: Is it necessary? and the short answer to this question is: NO!!! As a matter of fact, one of the steps I have developed to totally eliminate xylene from the histology lab refers to the clearing of stained sections, not only special stains (the so called HC and IHC) but the routine as well (the HE). Now, the secret to a successful drying of the stained slides is NOT to let them air dry because that will take not only too much time, but you can never be sure if the section is completely dry and if you add the mounting medium to a not completely dried section, you will have transparency problems. The correct way of doing that is by drying the stained sections during 5 minutes at 60?C in an oven. Under separate cover I am sending you something I published about your question and other aspects of how to completely eliminate xylene from ALL steps in the histology laboratory. Ren? J. From: Diana McCaig dmcc...@ckha.on.ca To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 10:32 AM Subject: [Histonet] air drying special stain slides rather than dehydrate and clear I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping.? Reason given is that the counterstain gets washed out.? Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: air drying special stain slides rather than
Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha N tnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrate and clear To: histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain; charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana -- Message: 17 Date: Tue, 11 Sep 2012 07:52:05 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] air drying special stain slides rather than dehydrate and clear To: Diana McCaig dmcc...@ckha.on.ca, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1347375125.72189.yahoomail...@web121405.mail.ne1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Diana: The most simple answer to your question is: Because that is the way it has been done for more than 150 years. The second question would be: Is it necessary? and the short answer to this question is: NO!!! As a matter of fact, one of the steps I have developed to totally eliminate xylene from the histology lab refers to the clearing of stained sections, not only special stains (the so called HC and IHC) but the routine as well (the HE). Now, the secret to a successful drying of the stained slides is NOT to let them air dry because that will take not only too much time, but you can never be sure if the section is completely dry and if you add the mounting medium to a not completely dried section, you will have
Re: [Histonet] RE: air drying special stain slides rather than
I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrateand clear To:histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain;charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana -- Message: 17 Date: Tue, 11 Sep 2012 07:52:05 -0700 (PDT) From: Rene J Buesarjbu...@yahoo.com Subject: Re: [Histonet] air drying special stain slides rather than
RE: [Histonet] RE: air drying special stain slides rather than
We have been using the oven dry method for special stains for about four years now and it works wonderfully. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: Tuesday, September 11, 2012 10:15 AM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrateand clear To:histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain;charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to
drying and coverslipping machines....RE: [Histonet] RE: air drying special stain slides rather than
If you dry the slides do you coverslip on a coverslipping machine - in which you have to put into xylene to run? Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Tuesday, September 11, 2012 10:23 AM To: E. Wayne Johnson; Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: RE: [Histonet] RE: air drying special stain slides rather than We have been using the oven dry method for special stains for about four years now and it works wonderfully. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: Tuesday, September 11, 2012 10:15 AM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana
[Histonet] Pronase antigen retrieval
Hello out there in Histoland, I have to do some bulk runs with Pronase antigen retrieval. Up until now the number of slides have been so few that I have been able to do the antigen retrieval by dropping pronase on each slide and incubating them in a humidified chamber at 37C for 20min. I now have so many slides that my manager has suggested that I make up large amounts of the Pronase (250ml) and submerge the slides. She suggested placing the container in the oven earlier to allow the Pronase to get to 37C before I add the slides. How long is the Pronase ok to be keep at 37C before I use it? How long in advance can I place it in the 37C before it looses its ability to be used as an antigen retrieval? Thanks for all your help, Eva Permaul HTSR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: air drying special stain slides rather than
Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: September-11-12 1:15 PM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrateand clear To:histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain;charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to
RE: [Histonet] RE: air drying special stain slides rather than
We use tape coverslipping. Some techs dip the slides in the xylene on stainer before adding them to the coverslipper and some just use the amount of xylene coming out of the drip on the coverslipper. Either works fine and we have had no problems. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, September 11, 2012 10:23 AM To: E. Wayne Johnson; Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Mayer, Toysha N Subject: RE: [Histonet] RE: air drying special stain slides rather than Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: September-11-12 1:15 PM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana
RE: [Histonet] RE: air drying special stain slides rather than
So you do not use as automated coverslipper? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Tuesday, September 11, 2012 1:23 PM To: E. Wayne Johnson; Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: RE: [Histonet] RE: air drying special stain slides rather than We have been using the oven dry method for special stains for about four years now and it works wonderfully. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: Tuesday, September 11, 2012 10:15 AM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message:
[Histonet] immunofluorescence on methyl methacrylate embedded human bone samples
Dear Histonetters, Would there be a problem with autofluorescence in bone when performing immunofluorescence on formalin-fixed methyl methacrylate-embedded human bone marrow trephines? Thanks, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX Website: http://mellanbycentre.dept.shef.ac.uk Tel: 00353114-2713337 (office) 00353114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechnician pay-max out
Hi, Thanks for your response and the others as well. I don't really think it's a lol situation here. I've seen uncertified professionals with good work ethic and quality results get mid 20s easy. While they may provide a guide for lab managers to refer to, thank God NSH doesn't set pay rates. This is interesting though, as it substantiates the feeling a lot of Histo professionals have that their work contribution is not only under appreciated but unappreciated considering the effect a quality slide has on diagnosing a serious disease. I believe a sharp individual who knows his/her skills and can demonstrate them effectively can negotiate higher pay. It seems hospitals pay less than a private lab would and over ten years successful experience should definitely garner well over 30. They question is whether they are strong enough to demand it via negotiation. Kim, what part of the country are you and what type of institution have you had the most exposure to? Again Thanks for your response. On Sep 11, 2012, at 12:36 PM, Kim Donadio one_angel_sec...@yahoo.com wrote: Low to mid 20's. Anything over mid 20' s should be 10 yrs and up in my opinion and from what I've seen. And that person that said 40$ a hour lol. I'd like to see that job Also I think nsh has a salary scale you might could look up. Good luck Sent from my iPhone On Sep 10, 2012, at 2:13 PM, Contact HistoCare cont...@histocare.com wrote: I hope I get some honest answers out there so here goes, Variables are: 5yr professionals both HT and non-cert with equal capabilities. And assuming routine histo duties (embedding, cutting, etc maybe light grossing) and for kicks lets say primarily working with animal tissue, Based on your personal experience, where can these individuals expect to max out as far as hourly pay? Sure, we know it could vary based on institution and location but there definitely is a ceiling. Or alternatively, what WON'T you pay this person regardless of experience. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dissecting pins
Does anyone know where I can find some large-head pins to use when pinning out large tissue specimens for fixation? We already use the large T-pins and the regular bulletin board push-pins are too small. We need something with a larger, easier to grip plastic head, and long (at least ½ inch) pin shaft. Thanks for your help (and hope it might be one of our 'contract vendors', or I won't be able to use them anyway... L) Kim Kimberly D. Kolman, HT, (ASCP) Diagnostics 115 VA Eastern Kansas Health Care System 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 ph: 913-682-2000 x 52537/52539 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: air drying special stain slides rather than
EWayne: All mounting media contains a so called solvent. Permount contains toluene (not as nasty as xylene) and will penetrate the section provided it is absolutely dehydrated (in an oven in this case). You just have to finish the HC (or IHC) protocol and pop-in the slides in the oven. Balsam of Canada (the resin) is dissolved in xylene (always) so the penetration is also assured. Under separate cover I am sending you my articles. Try this method, you will love it! René J. From: E. Wayne Johnson e...@pigsqq.org To: Rene J Buesa rjbu...@yahoo.com Cc: Mayer,Toysha N tnma...@mdanderson.org; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 1:14 PM Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrate and clear To:histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca
Re: drying and coverslipping machines....RE: [Histonet] RE: air drying special stain slides rather than
I have used Sakura film and Leica glass coverslippers (slides directly from the oven).. René J. From: Morken, Timothy timothy.mor...@ucsfmedctr.org To: Lori Harris lhar...@samhealth.org; E. Wayne Johnson e...@pigsqq.org; Rene J Buesa rjbu...@yahoo.com Cc: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu; Mayer, Toysha N tnma...@mdanderson.org Sent: Tuesday, September 11, 2012 1:30 PM Subject: drying and coverslipping machinesRE: [Histonet] RE: air drying special stain slides rather than If you dry the slides do you coverslip on a coverslipping machine - in which you have to put into xylene to run? Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Tuesday, September 11, 2012 10:23 AM To: E. Wayne Johnson; Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: RE: [Histonet] RE: air drying special stain slides rather than We have been using the oven dry method for special stains for about four years now and it works wonderfully. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: Tuesday, September 11, 2012 10:15 AM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would
Re: [Histonet] RE: air drying special stain slides rather than
You do not need to wet the dried slides in xylene before using the automted coverslipper. René J. From: Diana McCaig dmcc...@ckha.on.ca To: E. Wayne Johnson e...@pigsqq.org; Rene J Buesa rjbu...@yahoo.com Cc: histonet@lists.utsouthwestern.edu; Mayer, Toysha N tnma...@mdanderson.org Sent: Tuesday, September 11, 2012 1:23 PM Subject: RE: [Histonet] RE: air drying special stain slides rather than Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: September-11-12 1:15 PM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrate and clear To:histonet@lists.utsouthwestern.edu
[Histonet] Re: Histotechnician pay-max out
I think its safe to say I have never seen a 5 year tech make $40 a hour ( in a hospital or privatly ). And I think its rude to post my comment back on the forum when I sent it to you privatly. I would be interested if all the other managers out there could please direct us all to the $40 a hour jobs that only require 5 years of experiance. Everybody wants to raise our worth and pay rate. But lets be honest. Thanks From: Contact HistoCare cont...@histocare.com To: Kim Donadio one_angel_sec...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 2:13 PM Subject: Histotechnician pay-max out Hi, Thanks for your response and the others as well. I don't really think it's a lol situation here. I've seen uncertified professionals with good work ethic and quality results get mid 20s easy. While they may provide a guide for lab managers to refer to, thank God NSH doesn't set pay rates. This is interesting though, as it substantiates the feeling a lot of Histo professionals have that their work contribution is not only under appreciated but unappreciated considering the effect a quality slide has on diagnosing a serious disease. I believe a sharp individual who knows his/her skills and can demonstrate them effectively can negotiate higher pay. It seems hospitals pay less than a private lab would and over ten years successful experience should definitely garner well over 30. They question is whether they are strong enough to demand it via negotiation. Kim, what part of the country are you and what type of institution have you had the most exposure to? Again Thanks for your response. On Sep 11, 2012, at 12:36 PM, Kim Donadio one_angel_sec...@yahoo.com wrote: Low to mid 20's. Anything over mid 20' s should be 10 yrs and up in my opinion and from what I've seen. And that person that said 40$ a hour lol. I'd like to see that job Also I think nsh has a salary scale you might could look up. Good luck Sent from my iPhone On Sep 10, 2012, at 2:13 PM, Contact HistoCare cont...@histocare.com wrote: I hope I get some honest answers out there so here goes, Variables are: 5yr professionals both HT and non-cert with equal capabilities. And assuming routine histo duties (embedding, cutting, etc maybe light grossing) and for kicks lets say primarily working with animal tissue, Based on your personal experience, where can these individuals expect to max out as far as hourly pay? Sure, we know it could vary based on institution and location but there definitely is a ceiling. Or alternatively, what WON'T you pay this person regardless of experience. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 106, Issue 11
-- Well, actually that is something that I do not teach since that method is not on the BOC exam. I only teach what is on the exam and usual methods in laboratories. If the ASCP places it on the exam then I will cover it, but not until then, they go by what is in Carson's text. None of our past students have mentioned anything like that on the registry Rene, but not all of them have taken it this year. The Lean methodology that I was referring to is 'taking the waste out of procedures' to speed up the workflow, and adding the step of drying before coverslipping may slow things down. The number crunchers will look at it that way. Toysha Message: 6 Date: Tue, 11 Sep 2012 09:01:35 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] RE: air drying special stain slides rather than To: Mayer,Toysha N tnma...@mdanderson.org, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 1347379295.24904.yahoomail...@web121404.mail.ne1.yahoo.com Content-Type: text/plain; charset=utf-8 Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate ??? clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60??C for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed ??? processed ??? dried (usually at the same 60??C before staining) ??? stained and an additional step at 60??C to dry before cover-slipping is just that, an additional step at 60??C 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60??C cannot affect in a negative way to the work-flow 6- after staining you will oven??dry the sections. I think you should try the method instead. Ren?? J. From: Mayer,Toysha N tnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester.?? Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as?? the solvent of the counterstain. ?? Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved.?? The amount of time involved to blot and air dry the slides will affect the TAT for the specimen.?? 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5.?? Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than ?? dehydrate?? and clear To: histonet@lists.utsouthwestern.edu Message-ID: ?? dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain;?? charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping.?? Reason given is that the counterstain gets washed out.?? Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana -- Message: 17
Re: [Histonet] RE: Histonet Digest, Vol 106, Issue 11
Toysha: I think you have some confusions about what Lean is. The fundamental premise of Lean is that every step you do in the flow, will add value to your product, besides eliminating waste or muda as is called in Japanese. If you go from stained section to cover-slipped section you are adding value. Lets assume that the value is an arbitrary 2. If you dehydrate the sections and latter you clear them and use 3 alcohols and 2 xylenes, each of those steps will add 2/5 = 0.4 units of value per step. If, on the other hand, you oven dry your stained sections and cover-slip them after wards, meaning that you have just 1 step, that step has a value of 2/1 = 2. This means that oven drying the stained sections before cover-slipping (just 1 step) that step adds the same value (2) that your traditional 5 steps and that increases the Lean value by a factor of 5X Understand? If your crunchers look it that way, they do not understand Lean either! René J. From: Mayer,Toysha N tnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 3:38 PM Subject: [Histonet] RE: Histonet Digest, Vol 106, Issue 11 -- Well, actually that is something that I do not teach since that method is not on the BOC exam. I only teach what is on the exam and usual methods in laboratories. If the ASCP places it on the exam then I will cover it, but not until then, they go by what is in Carson's text. None of our past students have mentioned anything like that on the registry Rene, but not all of them have taken it this year. The Lean methodology that I was referring to is 'taking the waste out of procedures' to speed up the workflow, and adding the step of drying before coverslipping may slow things down. The number crunchers will look at it that way. Toysha Message: 6 Date: Tue, 11 Sep 2012 09:01:35 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] RE: air drying special stain slides rather than To: Mayer,Toysha N tnma...@mdanderson.org, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 1347379295.24904.yahoomail...@web121404.mail.ne1.yahoo.com Content-Type: text/plain; charset=utf-8 Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate ??? clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60??C for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed ??? processed ??? dried (usually at the same 60??C before staining) ??? stained and an additional step at 60??C to dry before cover-slipping is just that, an additional step at 60??C 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60??C cannot affect in a negative way to the work-flow 6- after staining you will oven??dry the sections. I think you should try the method instead. Ren?? J. From: Mayer,Toysha N tnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester.?? Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as?? the solvent of the counterstain. ?? Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved.?? The amount of time involved to blot and air dry the slides will affect the TAT for the specimen.?? 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5.?? Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor,
RE: [Histonet] Histotechnician pay-max out
Thanks for sharing. This is enlightening. I wish I was making what you suggest!Joelle Joelle Weaver MAOM, HTL (ASCP) QIHC From: cont...@histocare.com Date: Tue, 11 Sep 2012 13:13:40 -0500 To: one_angel_sec...@yahoo.com CC: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnician pay-max out Hi, Thanks for your response and the others as well. I don't really think it's a lol situation here. I've seen uncertified professionals with good work ethic and quality results get mid 20s easy. While they may provide a guide for lab managers to refer to, thank God NSH doesn't set pay rates. This is interesting though, as it substantiates the feeling a lot of Histo professionals have that their work contribution is not only under appreciated but unappreciated considering the effect a quality slide has on diagnosing a serious disease. I believe a sharp individual who knows his/her skills and can demonstrate them effectively can negotiate higher pay. It seems hospitals pay less than a private lab would and over ten years successful experience should definitely garner well over 30. They question is whether they are strong enough to demand it via negotiation. Kim, what part of the country are you and what type of institution have you had the most exposure to? Again Thanks for your response. On Sep 11, 2012, at 12:36 PM, Kim Donadio one_angel_sec...@yahoo.com wrote: Low to mid 20's. Anything over mid 20' s should be 10 yrs and up in my opinion and from what I've seen. And that person that said 40$ a hour lol. I'd like to see that job Also I think nsh has a salary scale you might could look up. Good luck Sent from my iPhone On Sep 10, 2012, at 2:13 PM, Contact HistoCare cont...@histocare.com wrote: I hope I get some honest answers out there so here goes, Variables are: 5yr professionals both HT and non-cert with equal capabilities. And assuming routine histo duties (embedding, cutting, etc maybe light grossing) and for kicks lets say primarily working with animal tissue, Based on your personal experience, where can these individuals expect to max out as far as hourly pay? Sure, we know it could vary based on institution and location but there definitely is a ceiling. Or alternatively, what WON'T you pay this person regardless of experience. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] air drying special stain slides rather than dehydrate and clear
Hi, My choice to air dry rather than dehydrate in ETOH xylene is based on the stain rather than the spooky xylene hazard boogyman. Yes, not using xylene if it is not really needed is not a bad idea, but the main reason I air dry some stains is the alcohols remove some of the stains. Ever have a beautiful Luxol Fast Blue bleach out on you? The most exasperating thing in the world! Generally stains that end in water can easily be air dried. Something alcoholic like eosin or Movat's Pentachrome ending in alcoholic saffron might as well be finished traditionally. I air dry any stain that is counterstained in Nuclear Fast Red, Light Green, Methyl Green. I have air dried IHCs with no ill effects too. Don't try it with fluorescents though, that would be bad ... and pointless. I don't put them in an oven. I set them at the front of the fume hood and go do something else for a few minutes. If I want to rush it I close the sash to increase the flow rate for a bit. (Of course it is opened back up right after so the draft works properly.) Amos On Tue, Sep 11, 2012 at 11:09 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrate and clear To: histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain; charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
