[Histonet] Tissue grossing
Hi all, How does one become qualified to gross tissue? A lot of the jobs I have been looking at call for being qualified to gross tissue, where I work right now the residents and Pathology assistants are the ones who gross the tissue. Thanks in advance for any help! Kristen Martin Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] murine endothelial makrers
&nb= sp;I have used Pharmingen anti cd31but make no mistake this can be finicky = and it would appear it should not be. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC On 09/06/12, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to [1]= histonet@lists.utsouthwestern.edu To subscribe or unsubscribe vi= a the World Wide Web, visit [2]http://lists.u= tsouthwestern.edu/mailman/listinfo/histonet or, via email, send a me= ssage with subject or body 'help' to [3]histonet-requ= e...@lists.utsouthwestern.edu You can reach the person managing t= he list at [4]histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specif= ic than "Re: Contents of Histonet digest..." Today's Topics:<= BR> 1. Re: Histonet Digest, Vol 106, Issue 4 (Madeleine Huey) 2. Ergo= nomics (Gagnon, Eric) 3. endothelial cell marker (Amos Brooks) 4. der= matologist volunteer needed - Belize Mission Project (Grantham, Andrea L= - (algranth)) 5. ImageJ : start help for muscle fiber count (Tora Barda= l) 6. RE: ImageJ : start help for muscle fiber count (Elizabeth Chlip= ala) 7. Canton, Ohio HT Position (Hale, Meredith) 8. Colorado HT (Hal= e, Meredith) --- --- Message: 1 Date: Wed, 5 Sep 2012 11:37:49 -07= 00 From: Madeleine Huey <[5]madeleineh...@gmail.com> Subje= ct: [Histonet] Re: Histonet Digest, Vol 106, Issue 4 To: [6]h= isto...@lists.utsouthwestern.edu Message-ID: < BR>Content-Type: text/plain; charset=ISO-8859-1 > Date: Wed, 5 = Sep 2012 10:04:58 + > From: "Bruijntjes, J.P. (Joost)" <[8]joost.bruijnt...@tno.triskelion.nl> > Subject: [Hist= onet] endothelial cell marker > To: "[9]histo...@lists.uts= outhwestern.edu" > <[10]Histonet@lists.utsouthwester= n.edu> > Message-ID: > > Con= tent-Type: text/plain; charset="us-ascii" > > Hi all ><= BR>> Is anyone of you familiar with an antibody directed against endothe= lial cells which can be applied on FFPE mouse tissues? > > Best= regards > Joost Bruijntjes Joost, AbCam sell Rabbit anti-CD31 = & will cross-react with Mouse FFPE tissues (Cat. # ab28364); [12]http://www.abcam.com/CD31-antibody-ab28364.html Hope= this help! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - El Camino= Hospital (Pathology) -- = Message: 2 Date: Wed, 5 Sep 2012 19:56:21 + From: "Gagnon, Eric" = <[13]gagn...@kgh.kari.net> Subject: [Histonet] Ergonomics To: = "[14]histonet@lists.utsouthwestern.edu" <[15]= histonet@lists.utsouthwestern.edu> Message-ID: <5F06C3AD0B2 7264CA20C[16]fa986c87882e31519...@exchangepv3.kgh.on.ca<= /A>> Content-Type: text/plain; charset="us-ascii" Hi Karen,<= BR> Here's a response to your question, as I haven't seen any others (?) While I haven't used the Newcomer handgrip device, I developed have DeQuer= vain's Tenosynovitis , a repetitive strain injury from turnin' the ol' micr= otome wheel for 28 or so years now. I would definitely recommend the foot p= edal, since you have a microtome equipped with one. Yes, there is some loss= of 'feeling' and it's a change from two hands on, but there is still the o= ption of taking the microtome 'off line' and turning the wheel manually for= difficult blocks. I can only speak for myself, but the more I used = the foot pedal, the more confident and at ease I became. Start off slowly, = working up towards more challenging blocks as soon as you can. Not that one= can ever let one's guard down with anything with a motor...car, microtome,= what have you. But in terms of ergonomics and saving your joints, I= would try anything and everything you have at your disposal. It's never to= o late to change for the better. Hope this helps, Eric Gagnon= MLT Histology Laboratory Kingston General Hospital, Kingston, Ont= ario, Canada -- Message: 3 Date: Wed, 5 Sep 2012 17:06:12 -0400 From: Amos Brooks <[17]amosbrooks@= gmail.com> Subject: [Histonet] endothelial cell marker To: [18]histonet@lists.utsouthwestern.edu, [19]joost.b= ruijnt...@tno.triskelion.nl Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, My favorite = by far is VonWillebrandt (spelling?) factor VIII. Dako has a really good= one (Cat# A0082) that works great in mouse tissue. CD31 is OK but it is= really finicky. CD34 works but F8 is easier and more reliable. Amos On Wed, Sep 5, 2012 at 1:01 PM, wrote: > Message: = 5 > Date: Wed, 5 Sep 2012 10:04:58 + > From: "Bruijntjes, J= .P. (Joost)" <[21]joost.bruijnt...@tno.triskelion.nl><= BR>> Subject: [Histonet] endothelial cell marker > To: "[22]Histonet@l
Re: [Histonet] RE: mouse testis in Bouins
From CDC - not quite a lab, but in Jan. 2002, this company was "melting" down the plastic from around capacitors, to regain the metals inside, by putting the capacitors in a heavy metal pot with acid, and leaving it overnight. The next day, the person went to remove the metal lid from the metal pot. Picric acid had formed, and a large explosion occurred. Look at the photos of the pot, and at the remains of the concrete building with a roof. 1 person killed, 1 severely injured, 5 others also injured. http://www.cdc.gov/niosh/face/stateface/nj/02nj003.html Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The views expressed are mine, and do not reflect on the hospital -Original Message- From: E. Wayne Johnson Sent: Friday, September 14, 2012 8:58 PM To: Jackie O'Connor Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: mouse testis in Bouins What danger of Picric Acid are you concerned with? Surely its not the hyped explosion hazards. We use picric acid and as inquisitive boys we have tried very hard to ignite it thinking it would be fun. We dried some down and wrapped it in aluminum foil and with appropriate protection outdoors beat it with a hammer. So very disappointing. We only made it flat. We tried heating some. It does burn pretty good but not really dramatically. We tried purifying and recrystallizing it and it still didnt do anything spectacular. Our conclusion that as fireworks, pure picric acid is pretty much a dud. I have done some reading about picric acid and it seems that in lab conditions a picric acid explosion is very unlikely maybe impossible even if the stuff is very dry indeed. We do keep our picric acid wet in a safe spot for storage. Some metal salts of picric acid are said to be much more sensitive. We havent made any lead picrate to play with since we are worried about aerosolizing the lead when it does explode or flash. There are some youtube movies about how to make explosive derivatives of picric acid. it seems that picric acid is just not a very good explosive, and that small amounts in free open air are unlikely to explode. I have been unable to find any reference to any lab accidents with picric acid. Does anyone have any information to the contrary? On 9/15/2012 7:55 AM, Jackie O'Connor wrote: As a GLP tox lab, we have done away with using Bouin's altogether - there is literature out there (somewhere - not handy now) that indicates Modified Davidson's fixative provides the same testicular detail of bouins, without the picric acid danger. We switched about 3-4 years ago, and our testicle experts are happy. I believe most labs are getting away from Bouins. Jackie O' -Original Message- From: Frances Elizabeth Barron To: histonet Sent: Fri, Sep 14, 2012 12:21 pm Subject: [Histonet] RE: mouse testis in Bouins Hi Margaret, Our protocol for whole mouse embryos E14.5-E18.5 was to fix in Bouin's for 5-7 days at room temp (I have gone longer, but it isn't exactly recommended). Most of the length of time, however, was to compensate for the large tissue size and need for good penetration. I'm not sure how that converts to your particular tissue of interest. For long term storage, John Shelton at UT Southwestern (who did our vacuum processing for large embryos) told me that it was preferred to put them in 1% neutral buffered formalin and store them at room temp. We had previously been storing them in 70% EtOH, but John said that the long exposure to EtOH leads to excessive drying of the tissue and ultimately brittleness if used later. I'm assuming this thought could be applied to any tissue piece, but I don't have enough experience to really know. We have successfully gotten beautiful paraffin sections from 3mo-1year samples that have been stored this way. I'm hoping this will be of some help to you, and perhaps others in the list can comment. Best of luck, ~Francie *** Francie Barron, Ph.D. Postdoctoral Fellow, Joseph Wu Lab Stanford University School of Medicine Lorry I. Lokey Stem Cell Research Building 265 Campus Drive, Room G1105 Stanford, CA 94305-5454 Phone: (650) 724-5564 or (650) 724-9240 Fax: (650) 736-0234 *** Message: 7 Date: Fri, 14 Sep 2012 10:06:33 -0300 From: "Margaret Horne" Subject: [Histonet] mouse testis in Bouins To: Message-ID:<505301a902d100018...@oes-grpwise.novell.upei.ca> Content-Type: text/plain; charset="us-ascii" Hello Everyone, I am asking this for a friend. How long can mouse testis be kept in Bouins without distortion of cell morphology? Days? weeks? months? years? I noticed in the Archives that many people fix in Bouins , rinse, then store in 70% EtOH. This is preferable I assume. Again, how long is ok? Thanks in advance for the sharing of your accumulated wisdom, Margaret
[Histonet] Cd31
Hi Which endothelial marker would you use for rat tissue? I am really interested to see what you all say. Thanks in advance Helen Sent from my BlackBerry® wireless device___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
